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WO2000012704A3 - Enhanced functional expression of g protein-coupled receptors - Google Patents

Enhanced functional expression of g protein-coupled receptors Download PDF

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Publication number
WO2000012704A3
WO2000012704A3 PCT/US1999/020011 US9920011W WO0012704A3 WO 2000012704 A3 WO2000012704 A3 WO 2000012704A3 US 9920011 W US9920011 W US 9920011W WO 0012704 A3 WO0012704 A3 WO 0012704A3
Authority
WO
WIPO (PCT)
Prior art keywords
protein
coupled receptors
functional expression
enhanced functional
receptors
Prior art date
Application number
PCT/US1999/020011
Other languages
French (fr)
Other versions
WO2000012704A9 (en
WO2000012704A2 (en
Inventor
Mark Henry Pausch
Margaret Lai
Sanford Silverman
Camelia Birsan
William Baumbauch
Eugene Tseng
Eileen Marie Kajkowski
Bradley Alton Ozenberger
Original Assignee
Mark Henry Pausch
Margaret Lai
Sanford Silverman
Camelia Birsan
William Baumbauch
Eugene Tseng
Eileen Marie Kajkowski
Bradley Alton Ozenberger
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mark Henry Pausch, Margaret Lai, Sanford Silverman, Camelia Birsan, William Baumbauch, Eugene Tseng, Eileen Marie Kajkowski, Bradley Alton Ozenberger filed Critical Mark Henry Pausch
Priority to JP2000567691A priority Critical patent/JP2002523090A/en
Priority to AU57009/99A priority patent/AU5700999A/en
Priority to EP99944033A priority patent/EP1112359A2/en
Priority to CA002343046A priority patent/CA2343046A1/en
Publication of WO2000012704A2 publication Critical patent/WO2000012704A2/en
Publication of WO2000012704A9 publication Critical patent/WO2000012704A9/en
Publication of WO2000012704A3 publication Critical patent/WO2000012704A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C07K14/395—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts from Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4722—G-proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70571—Receptors; Cell surface antigens; Cell surface determinants for neuromediators, e.g. serotonin receptor, dopamine receptor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/72—Receptors; Cell surface antigens; Cell surface determinants for hormones
    • C07K14/723—G protein coupled receptor, e.g. TSHR-thyrotropin-receptor, LH/hCG receptor, FSH receptor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Immunology (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)
  • Neurology (AREA)
  • Engineering & Computer Science (AREA)
  • Endocrinology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

This invention relates to constitutively active mutant G protein-coupled receptors, yeast cell expressing such receptors, vectors useful for making such cells, and methods of making and using same.
PCT/US1999/020011 1998-09-01 1999-09-01 Enhanced functional expression of g protein-coupled receptors WO2000012704A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP2000567691A JP2002523090A (en) 1998-09-01 1999-09-01 Enhanced functional expression of heterologous G protein-coupled receptors
AU57009/99A AU5700999A (en) 1998-09-01 1999-09-01 Enhanced functional expression of g protein-coupled receptors
EP99944033A EP1112359A2 (en) 1998-09-01 1999-09-01 Enhanced functional expression of g protein-coupled receptors
CA002343046A CA2343046A1 (en) 1998-09-01 1999-09-01 Enhanced functional expression of g protein-coupled receptors

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US9870498P 1998-09-01 1998-09-01
US60/098,704 1998-09-01

Publications (3)

Publication Number Publication Date
WO2000012704A2 WO2000012704A2 (en) 2000-03-09
WO2000012704A9 WO2000012704A9 (en) 2000-06-08
WO2000012704A3 true WO2000012704A3 (en) 2000-11-30

Family

ID=22270543

Family Applications (2)

Application Number Title Priority Date Filing Date
PCT/US1999/020013 WO2000012705A2 (en) 1998-09-01 1999-09-01 Methods for improving the function of heterologous g protein-coupled receptors
PCT/US1999/020011 WO2000012704A2 (en) 1998-09-01 1999-09-01 Enhanced functional expression of g protein-coupled receptors

Family Applications Before (1)

Application Number Title Priority Date Filing Date
PCT/US1999/020013 WO2000012705A2 (en) 1998-09-01 1999-09-01 Methods for improving the function of heterologous g protein-coupled receptors

Country Status (5)

Country Link
EP (2) EP1112359A2 (en)
JP (2) JP2002523090A (en)
AU (2) AU756244B2 (en)
CA (2) CA2343046A1 (en)
WO (2) WO2000012705A2 (en)

Families Citing this family (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6528271B1 (en) 1997-06-05 2003-03-04 Duke University Inhibition of βarrestin mediated effects prolongs and potentiates opioid receptor-mediated analgesia
US5891646A (en) 1997-06-05 1999-04-06 Duke University Methods of assaying receptor activity and constructs useful in such methods
US7541151B2 (en) 1997-06-05 2009-06-02 Duke University Single-cell biosensor for the measurement of GPCR ligands in a test sample
EP1276371A2 (en) 2000-04-24 2003-01-22 Wyeth Transgenic animal
CA2426859A1 (en) * 2000-10-26 2002-05-02 The University Of British Columbia A functional assay for g-protein-coupled receptors based on insect cells
EP1331228B1 (en) * 2000-11-01 2007-03-21 Astellas Pharma Inc. Method of screening antiplatelet
US7163800B2 (en) 2000-11-03 2007-01-16 Molecular Devices Corporation Methods of screening compositions for G protein-coupled receptor desensitization inhibitory activity
US7018812B2 (en) 2000-11-03 2006-03-28 Duke University Modified G-protein coupled receptors
DE10056899A1 (en) 2000-11-16 2002-05-29 Aventis Pharma Gmbh New promoter from Saccharomyces cerevisiae YNL279W gene, useful e.g. for identifying modulators of G protein-coupled receptors, is responsive to pheromone alpha
WO2002045503A2 (en) * 2000-12-06 2002-06-13 Deltagen, Inc. Transgenic mice containing targeted gene disruptions
GB0030038D0 (en) * 2000-12-08 2001-01-24 Univ Warwick Yeast-based assay
US7279324B2 (en) 2001-01-23 2007-10-09 Duke University Nucleic acid encoding G-protein coupled receptor with modified DRY motif
EP1336655A1 (en) * 2002-01-12 2003-08-20 Aventis Pharma Deutschland GmbH Method of identifying protein CAMS (constitutively active mutants)
DE10233516A1 (en) * 2002-07-23 2004-02-12 Aventis Pharma Deutschland Gmbh Cellular method for identifying modulators of target molecules, useful in screening for pharmaceuticals, from effects of test compounds on both cell growth and reporter expression
JP4977012B2 (en) * 2005-03-29 2012-07-18 Ｂｉｏ−ｅｎｅｒｇｙ株式会社 Screening method for receptor binding substance
EP1871362A2 (en) 2005-04-27 2008-01-02 Arena Pharmaceuticals, Inc. Combination therapy for the treatment of obesity and diabetes and conditions related thereto and for the treatment of conditions ameliorated by increasing a blood glp-1 level
NZ564759A (en) 2005-06-30 2011-08-26 Prosidion Ltd GPCR agonists
JP4521838B2 (en) 2006-04-11 2010-08-11 アリーナ ファーマシューティカルズ， インコーポレイテッド Methods of using the GPR119 receptor to identify compounds that are useful for increasing bone mass in an individual
PL2114933T3 (en) 2007-01-04 2012-02-29 Prosidion Ltd Piperidine gpcr agonists
AR064735A1 (en) 2007-01-04 2009-04-22 Prosidion Ltd GPCR AGONISTS AND PHARMACEUTICAL COMPOSITION BASED ON THE COMPOUND
AR064736A1 (en) 2007-01-04 2009-04-22 Prosidion Ltd GPCR AGONISTS
AU2008228085B2 (en) * 2007-03-22 2011-12-08 Nxera Pharma Uk Limited Mutant G-protein coupled receptors and methods for selecting them
US20100286112A1 (en) 2007-09-10 2010-11-11 Oscar Barba Compounds for the treatment of metabolic disorders
GB0720389D0 (en) 2007-10-18 2008-11-12 Prosidion Ltd G-Protein Coupled Receptor Agonists
GB0720390D0 (en) 2007-10-18 2007-11-28 Prosidion Ltd G-Protein coupled receptor agonists
EP2146210A1 (en) 2008-04-07 2010-01-20 Arena Pharmaceuticals, Inc. Methods of using A G protein-coupled receptor to identify peptide YY (PYY) secretagogues and compounds useful in the treatment of conditions modulated by PYY
GB0904284D0 (en) 2009-03-12 2009-04-22 Prosidion Ltd Compounds for the treatment of metabolic disorders
GB0904285D0 (en) 2009-03-12 2009-04-22 Prosidion Ltd Compounds for the treatment of metabolic disorders
GB0904287D0 (en) 2009-03-12 2009-04-22 Prosidion Ltd Compounds for the treatment of metabolic disorders
JP7422498B2 (en) * 2019-07-25 2024-01-26 芳武 村山 Peptides, cell migration inhibitors, cell invasion inhibitors, cancer cell metastasis inhibitors, and pharmaceuticals

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995021925A1 (en) * 1994-02-14 1995-08-17 American Cyanamid Company Heterologous g protein coupled receptors expressed in yeast, their fusion with g proteins and use thereof in bioassay
WO1996000739A1 (en) * 1994-06-29 1996-01-11 Merck & Co., Inc. Modified g-protein coupled receptors
WO1998038217A1 (en) * 1997-02-27 1998-09-03 Milt Teitler Constitutively activated serotonin receptors

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995021925A1 (en) * 1994-02-14 1995-08-17 American Cyanamid Company Heterologous g protein coupled receptors expressed in yeast, their fusion with g proteins and use thereof in bioassay
WO1996000739A1 (en) * 1994-06-29 1996-01-11 Merck & Co., Inc. Modified g-protein coupled receptors
WO1998038217A1 (en) * 1997-02-27 1998-09-03 Milt Teitler Constitutively activated serotonin receptors

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
A. SCHEER ET AL: "Constitutively active G-protein-coupled receptors: Potential mechanisms of receptor activation", JOURNAL OF RECEPTOR & SIGNAL TRANSDUCTION RESEARCH, vol. 17, no. 1-3, 1997, pages 57 - 73, XP000867531 *
EGGERICKX D ET AL: "MOLECULAR CLONING OF AN ORPHAN G-PROTEIN-COUPLED RECEPTOR THAT CONSTITUTIVELY ACTIVATES ADENYLATE CYCLASE", BIOCHEMICAL JOURNAL,GB,PORTLAND PRESS, LONDON, vol. 309, 1995, pages 837 - 843, XP002912497, ISSN: 0264-6021 *
M.A. KJELSBERG ET AL: "Constitutive activation of the alpha1B-adrenergic receptor by all amino acid substitutions at a single site", JOURNAL OF BIOLOGICAL CHEMISTRY., vol. 267, no. 3, 25 January 1992 (1992-01-25), AMERICAN SOCIETY OF BIOLOGICAL CHEMISTS, BALTIMORE, MD., US, pages 1430 - 1433, XP002135768, ISSN: 0021-9258 *
P. SAMAMA ET AL: "A mutation-induced activated state of the beta2-adrenergic receptor", JOURNAL OF BIOLOGICAL CHEMISTRY., vol. 268, no. 7, 5 March 1993 (1993-03-05), AMERICAN SOCIETY OF BIOLOGICAL CHEMISTS, BALTIMORE, MD., US, pages 4625 - 4636, XP002135769, ISSN: 0021-9258 *
PAUSCH M H ET AL: "HETEROLOGOUS G PROTEIN-COUPLED RECEPTORS EXPRESSED IN SACCHAROMYCES CEREVISIAE: METHODS FOR GENETIC ANALYSIS AND LIGAND IDENTIFICATION", IDENTIFICATION AND EXPRESSION OF G-PROTEIN-COUPLED RECEPTORS. CHAPTER II, 26 May 1999 (1999-05-26), Editedby Kevin R. Lynch, Wley-Liss, Inc., pages 196 - 212, XP000869632 *
PAUSCH M H: "G-protein-coupled receptors in Saccharomyces cerevisiae: high-throughput screening assays for drug discovery", TRENDS IN BIOTECHNOLOGY,GB,ELSEVIER PUBLICATIONS, CAMBRIDGE, vol. 15, no. 12, 1 December 1997 (1997-12-01), pages 487 - 494, XP004097426, ISSN: 0167-7799 *
PAUWELS P J ET AL: "REVIEW:AMINO ACID DOMAINS INVOLVED IN CONSTITUTIVE ACTIVATION OF G-PROTEIN-COUPLED RECEPTORS", MOLECULAR NEUROBIOLOGY,US,HUMANA PRESS, vol. 17, no. 1/03, 29 December 1998 (1998-12-29), pages 109 - 135, XP000866477, ISSN: 0893-7648 *

Also Published As

Publication number Publication date
WO2000012705A2 (en) 2000-03-09
JP2002523091A (en) 2002-07-30
CA2343046A1 (en) 2000-03-09
WO2000012705A9 (en) 2000-07-13
CA2344591A1 (en) 2000-03-09
EP1123391A2 (en) 2001-08-16
EP1112359A2 (en) 2001-07-04
AU756244B2 (en) 2003-01-09
WO2000012705A3 (en) 2000-10-05
WO2000012704A9 (en) 2000-06-08
AU5700999A (en) 2000-03-21
WO2000012704A2 (en) 2000-03-09
JP2002523090A (en) 2002-07-30
AU5701199A (en) 2000-03-21

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