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WO2000065039A3 - Identification d'elements de controle d'adn reagissant a des stimuli specifiques - Google Patents

Identification d'elements de controle d'adn reagissant a des stimuli specifiques Download PDF

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Publication number
WO2000065039A3
WO2000065039A3 PCT/US2000/011460 US0011460W WO0065039A3 WO 2000065039 A3 WO2000065039 A3 WO 2000065039A3 US 0011460 W US0011460 W US 0011460W WO 0065039 A3 WO0065039 A3 WO 0065039A3
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sar
mutants
genes
expression
gene
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PCT/US2000/011460
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WO2000065039A2 (fr
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Klaus Maleck
Kay Ann Lawton
Robert Arthur Dietrich
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Novartis Ag
Klaus Maleck
Kay Ann Lawton
Robert Arthur Dietrich
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Application filed by Novartis Ag, Klaus Maleck, Kay Ann Lawton, Robert Arthur Dietrich filed Critical Novartis Ag
Priority to AU46754/00A priority Critical patent/AU4675400A/en
Publication of WO2000065039A2 publication Critical patent/WO2000065039A2/fr
Publication of WO2000065039A3 publication Critical patent/WO2000065039A3/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1072—Differential gene expression library synthesis, e.g. subtracted libraries, differential screening
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237—Externally regulated expression systems
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237—Externally regulated expression systems
    • C12N15/8239—Externally regulated expression systems pathogen inducible
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters

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  • Health & Medical Sciences (AREA)
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  • Life Sciences & Earth Sciences (AREA)
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  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
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  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Communicable Diseases (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Pour identifier les gènes dont les protéines interviennent dans la régulation de la résistance systémique acquise en aval de la mort cellulaire, on a utilisé un crible visant à isoler les mutants qui expriment cette résistance de manière constitutive. Le promoteur PR-1, qui est le marqueur le plus fiable pour une apparition de ladite résistance, a été cloné devant le gène rapporteur de luciférase de luciole (Photinus pyralis) et transformé en Arabidopsis. Une ligne transgénique à forme d'expression de luciférase parallèle au gène PR-1 endogène a été identifiée et soumise à une mutagenèse EMS pour isoler les mutants qui expriment de manière constitutive le gène de luciférase PR-1. Des techniques biochimiques, cytologiques, pathologiques et génétiques ont été employées pour caractériser plus avant les mutants et pour mettre en évidence l'isolation des mutants de la résistance systémique acquise en aval de la mort cellulaire. Cette caractérisation permet de faire la distinction entre les classes de mutants cim et de décrire les modifications physiologiques intervenant durant la phase de maintenance de la résistance systémique acquise. En outre, l'utilisation de puces à jeu ordonné de microéchantillons a permis d'examiner simultanément l'ensemble du génome végétal pour déceler les gènes dont l'expression se modifie en réponse à des facteurs biotiques ou abiotiques. La comparaison des modifications de l'expression génique sur différents traitements a permis d'identifier des groupes de gènes corégulés (régulons), et on a examiné les séquences génomiques de gènes dans un régulon pour identifier des motifs de séquences communes pouvant vraisemblablement tenir lieu d'éléments régulateurs. L'approche utilisée fait appel à une conception expérimentale fondée sur la biologie du système à l'étude, en combinaison avec la bioinformatique visant elle à analyser les résultats.
PCT/US2000/011460 1999-04-28 2000-04-28 Identification d'elements de controle d'adn reagissant a des stimuli specifiques WO2000065039A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU46754/00A AU4675400A (en) 1999-04-28 2000-04-28 Identification of dna control elements responsive to specific stimuli

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US13143399P 1999-04-28 1999-04-28
US60/131,433 1999-04-28
US14466999P 1999-07-19 1999-07-19
US60/144,669 1999-07-19

Publications (2)

Publication Number Publication Date
WO2000065039A2 WO2000065039A2 (fr) 2000-11-02
WO2000065039A3 true WO2000065039A3 (fr) 2001-02-08

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AU (1) AU4675400A (fr)
WO (1) WO2000065039A2 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109234284B (zh) * 2018-09-14 2021-04-09 昆明理工大学 一种三七类甜蛋白基因PnTLP5及应用

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU1179001A (en) * 1999-10-13 2001-04-23 Plant Research International B.V. Method for detecting differences in gene expression between wild type plants andnewly developed plants
AU2001262943A1 (en) 2000-04-14 2001-10-30 Metabolon, Inc. Methods for drug discovery, disease treatment, and diagnosis using metabolomics
US7329489B2 (en) 2000-04-14 2008-02-12 Matabolon, Inc. Methods for drug discovery, disease treatment, and diagnosis using metabolomics
US20040076954A1 (en) * 2001-03-12 2004-04-22 Irm, Llc Genomics-driven high speed cellular assays, development thereof, and collections of cellular reporters
US6943242B2 (en) * 2001-05-07 2005-09-13 Amersham Biosciences Corp. Design of artificial genes for use as controls in gene expression analysis systems
WO2008033575A2 (fr) 2006-09-15 2008-03-20 Metabolon, Inc. Procédés d'identification de cheminements biochimiques

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5851766A (en) * 1995-05-31 1998-12-22 Novartis Finance Corporation Process for isolating chemically regulatable DNA sequences
DE19860313A1 (de) * 1998-12-24 2000-06-29 Friedrich Felsenstein Verfahren zur Erkennung und Charakterisierung von Wirkstoffen gegen Pflanzen-Pathogene

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5851766A (en) * 1995-05-31 1998-12-22 Novartis Finance Corporation Process for isolating chemically regulatable DNA sequences
DE19860313A1 (de) * 1998-12-24 2000-06-29 Friedrich Felsenstein Verfahren zur Erkennung und Charakterisierung von Wirkstoffen gegen Pflanzen-Pathogene

Non-Patent Citations (10)

* Cited by examiner, † Cited by third party
Title
DESPREZ THIERRY ET AL: "Differential gene expression in Arabidopsis monitored using cDNA arrays.", PLANT JOURNAL, vol. 14, no. 5, June 1998 (1998-06-01), pages 643 - 652, XP000960485, ISSN: 0960-7412 *
MOLINA ANTONIO ET AL: "Impaired fungicide activity in plants blocked in disease resistance signal transduction.", PLANT CELL, vol. 10, no. 11, November 1998 (1998-11-01), pages 1903 - 1914, XP000885732, ISSN: 1040-4651 *
RAFALSKI J ANTONI ET AL: "New experimental and computational approaches to the analysis of gene expression.", ACTA BIOCHIMICA POLONICA, vol. 45, no. 4, 1998, pages 929 - 934, XP000952158, ISSN: 0001-527X *
RAMONELL K M ET AL: "Microarray dissection of systemic acquired resistance in Arabidopsis plants infected with powdery mildew.", PHYTOPATHOLOGY, vol. 90, no. 6 Supplement, June 2000 (2000-06-01), Annual Meeting of the American Phytopathological Society;New Orleans, Louisiana, USA; August 12-16, 2000, pages S63, XP000964562, ISSN: 0031-949X *
RUAN YIJUN ET AL: "Towards Arabidopsis genome analysis: Monitoring expression profiles of 1400 genes using cDNA microarrays.", PLANT JOURNAL, vol. 15, no. 6, 1998, pages 821 - 833, XP000960486, ISSN: 0960-7412 *
SCHENA M ET AL: "QUANTITATIVE MONITORING OF GENE EXPRESSION PATTERNS WITH A COMPLEMENTARY DNA MICROARRAY", SCIENCE,US,AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE,, vol. 270, no. 5235, 20 October 1995 (1995-10-20), pages 467 - 470, XP000644675, ISSN: 0036-8075 *
SCHENK P M ET AL: "Coordinated plant defense responses in Arabidopsis revealed by microarray analysis", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, (10 OCT 2000) VOL. 97, NO. 21, PP. 11655-11660. PUBLISHER: NATL ACAD SCIENCES, 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418. ISSN: 0027-8424., XP002153163 *
SOMERVILLE, S. C. ET AL: "Alternate methods of gene discovery - the candidate EST approach and DNA microarrays", CELLULAR INTEGRATION OF SIGNALLING PATHWAYS IN PLANT DEVELOPMENT. PROCEEDINGS OF THE NATO ADVANCED STUDY INSTITUTE, ACQUA FREDDA DI MARATEA, ITALY, 20-30 MAY, 1997, (1998) PP. 297-309. NATO ASI SERIES. SERIES H: CELL BIOLOGY VOL. 104., Department of Plant Biology, Carnegie Institution of Washington, Stanford, CA 94305, USA, XP000964578 *
SPELLMAN ET AL: "COMPREHENSIVE IDENTIFICATION OF CELL CYCLE-REGULATED GENES OF THE YEAST SACCHAROMYCES CEREVISIAE BY MICROARRAY HYBRIDIZATION", MOLECULAR BIOLOGY OF THE CELL,US,BETHESDA, MD, vol. 9, December 1998 (1998-12-01), pages 3273 - 3297, XP002143737, ISSN: 1059-1524 *
WARD E R ET AL: "COORDINATE GENE ACTIVITY IN RESPONSE TO AGENTS THAT INDUCE SYSTEMIC ACQUIRED RESISTANCE", PLANT CELL, vol. 3, no. 10, 1991, pages 1085 - 1094, XP002134008, ISSN: 1040-4651 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109234284B (zh) * 2018-09-14 2021-04-09 昆明理工大学 一种三七类甜蛋白基因PnTLP5及应用

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WO2000065039A2 (fr) 2000-11-02

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