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WO2000065039A3 - Identification of dna control elements responsive to specific stimuli - Google Patents

Identification of dna control elements responsive to specific stimuli Download PDF

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Publication number
WO2000065039A3
WO2000065039A3 PCT/US2000/011460 US0011460W WO0065039A3 WO 2000065039 A3 WO2000065039 A3 WO 2000065039A3 US 0011460 W US0011460 W US 0011460W WO 0065039 A3 WO0065039 A3 WO 0065039A3
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WO
WIPO (PCT)
Prior art keywords
sar
mutants
genes
expression
gene
Prior art date
Application number
PCT/US2000/011460
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French (fr)
Other versions
WO2000065039A2 (en
Inventor
Klaus Maleck
Kay Ann Lawton
Robert Arthur Dietrich
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Novartis Ag
Klaus Maleck
Kay Ann Lawton
Robert Arthur Dietrich
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novartis Ag, Klaus Maleck, Kay Ann Lawton, Robert Arthur Dietrich filed Critical Novartis Ag
Priority to AU46754/00A priority Critical patent/AU4675400A/en
Publication of WO2000065039A2 publication Critical patent/WO2000065039A2/en
Publication of WO2000065039A3 publication Critical patent/WO2000065039A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1072—Differential gene expression library synthesis, e.g. subtracted libraries, differential screening
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237—Externally regulated expression systems
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237—Externally regulated expression systems
    • C12N15/8239—Externally regulated expression systems pathogen inducible
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Communicable Diseases (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

In order to identify genes whose proteins are involved in the regulation of SAR downstream of cell death, a screen was carried out to isolate mutants that constitutively express SAR. The PR-1 promoter, the most reliable marker for the onset of SAR, was cloned in front of the firefly (Photinus pyralis) luciferase reporter gene and transformed into Arabidopsis. A transgenic line with a luciferase expression pattern that paralleled expression of the endogenous PR-1 gene was identified and subjected to EMS mutagenesis to isolate mutants that constitutively expressed the PR-1/luciferase gene. Biochemical, cytological, pathological, and genetic methods were employed to further characterize the mutants and to prove the isolation of SAR mutants downstream of cell death. This characterization enables one to distinguish between classes of cim mutants, and to describe physiological changes that occur during the maintenance phase of SAR. Furthermore, utilizing microarray chips, the entire plant genome was simultaneously surveyed for genes that change in expression in response to biotic and abiotic factors. By comparing gene expression changes across various treatments, groups of co-regulated genes (regulons) were identified and the genomic sequences of genes within a regulon were examined to identify common sequence motifs that are likely to act as regulatory elements. This approach used experimental design based upon the biology of the study system in combination with bioinformatics to analyze the results.
PCT/US2000/011460 1999-04-28 2000-04-28 Identification of dna control elements responsive to specific stimuli WO2000065039A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU46754/00A AU4675400A (en) 1999-04-28 2000-04-28 Identification of dna control elements responsive to specific stimuli

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US13143399P 1999-04-28 1999-04-28
US60/131,433 1999-04-28
US14466999P 1999-07-19 1999-07-19
US60/144,669 1999-07-19

Publications (2)

Publication Number Publication Date
WO2000065039A2 WO2000065039A2 (en) 2000-11-02
WO2000065039A3 true WO2000065039A3 (en) 2001-02-08

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/011460 WO2000065039A2 (en) 1999-04-28 2000-04-28 Identification of dna control elements responsive to specific stimuli

Country Status (2)

Country Link
AU (1) AU4675400A (en)
WO (1) WO2000065039A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109234284B (en) * 2018-09-14 2021-04-09 昆明理工大学 A kind of Panax notoginseng sweet protein gene PnTLP5 and its application

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU1179001A (en) * 1999-10-13 2001-04-23 Plant Research International B.V. Method for detecting differences in gene expression between wild type plants andnewly developed plants
AU2001262943A1 (en) 2000-04-14 2001-10-30 Metabolon, Inc. Methods for drug discovery, disease treatment, and diagnosis using metabolomics
US7329489B2 (en) 2000-04-14 2008-02-12 Matabolon, Inc. Methods for drug discovery, disease treatment, and diagnosis using metabolomics
US20040076954A1 (en) * 2001-03-12 2004-04-22 Irm, Llc Genomics-driven high speed cellular assays, development thereof, and collections of cellular reporters
US6943242B2 (en) * 2001-05-07 2005-09-13 Amersham Biosciences Corp. Design of artificial genes for use as controls in gene expression analysis systems
WO2008033575A2 (en) 2006-09-15 2008-03-20 Metabolon, Inc. Methods of identifying biochemical pathways

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5851766A (en) * 1995-05-31 1998-12-22 Novartis Finance Corporation Process for isolating chemically regulatable DNA sequences
DE19860313A1 (en) * 1998-12-24 2000-06-29 Friedrich Felsenstein Screening effectiveness of agents against plant pathogens at the molecular level by determining altered gene expression

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5851766A (en) * 1995-05-31 1998-12-22 Novartis Finance Corporation Process for isolating chemically regulatable DNA sequences
DE19860313A1 (en) * 1998-12-24 2000-06-29 Friedrich Felsenstein Screening effectiveness of agents against plant pathogens at the molecular level by determining altered gene expression

Non-Patent Citations (10)

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Title
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RAFALSKI J ANTONI ET AL: "New experimental and computational approaches to the analysis of gene expression.", ACTA BIOCHIMICA POLONICA, vol. 45, no. 4, 1998, pages 929 - 934, XP000952158, ISSN: 0001-527X *
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109234284B (en) * 2018-09-14 2021-04-09 昆明理工大学 A kind of Panax notoginseng sweet protein gene PnTLP5 and its application

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Publication number Publication date
AU4675400A (en) 2000-11-10
WO2000065039A2 (en) 2000-11-02

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