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WO1999011818A8 - Method for detecting highly functional polypeptides or nucleic acids - Google Patents

Method for detecting highly functional polypeptides or nucleic acids

Info

Publication number
WO1999011818A8
WO1999011818A8 PCT/JP1998/003854 JP9803854W WO9911818A8 WO 1999011818 A8 WO1999011818 A8 WO 1999011818A8 JP 9803854 W JP9803854 W JP 9803854W WO 9911818 A8 WO9911818 A8 WO 9911818A8
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acids
polypeptides
fitness
synthesizing
synthesized
Prior art date
Application number
PCT/JP1998/003854
Other languages
French (fr)
Japanese (ja)
Other versions
WO1999011818A1 (en
Inventor
Isao Karube
Yoichi Okabe
Koichi Sumikura
Original Assignee
Isao Karube
Yoichi Okabe
Koichi Sumikura
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Isao Karube, Yoichi Okabe, Koichi Sumikura filed Critical Isao Karube
Priority to AU88867/98A priority Critical patent/AU8886798A/en
Publication of WO1999011818A1 publication Critical patent/WO1999011818A1/en
Publication of WO1999011818A8 publication Critical patent/WO1999011818A8/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Ecology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

A molecular design method which fundamentally comprises artificially reconstructing genes by means of exon shuffle among a number of individuals. More particularly, this method comprises the steps of (a) synthesizing a number of polypeptides or nucleic acids differing in sequence from each other; (b) measuring the fitness of the synthesized polypeptides or nucleic acids on the laboratory level, (c) ranking the polypeptides or nucleic acids synthesized in step (a) depending on the fitness; (d) preparing a 'shuffling library' of the sequences obtained by shuffling the partial structures among individuals selected depending on the rank; (e) synthesizing polypeptides or nucleic acids belonging to the above library; and (f) repeating the procedures of steps (b) to (e) arbitrary times with the use of the polypeptides or nucleic acids obtained in step (e). By using this method, molecules can be evolved drastically and quickly as compared with the conventional genetic algorithm, which makes it possible to efficiently detect functional polymers, in particular, polypeptides or nucleic acids having specific functions and having novel structures which never occur in nature.
PCT/JP1998/003854 1997-08-28 1998-08-28 Method for detecting highly functional polypeptides or nucleic acids WO1999011818A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU88867/98A AU8886798A (en) 1997-08-28 1998-08-28 Method for detecting highly functional polypeptides or nucleic acids

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP24967997 1997-08-28
JP9/249679 1997-08-28

Publications (2)

Publication Number Publication Date
WO1999011818A1 WO1999011818A1 (en) 1999-03-11
WO1999011818A8 true WO1999011818A8 (en) 1999-04-15

Family

ID=17196601

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP1998/003854 WO1999011818A1 (en) 1997-08-28 1998-08-28 Method for detecting highly functional polypeptides or nucleic acids

Country Status (2)

Country Link
AU (1) AU8886798A (en)
WO (1) WO1999011818A1 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG121902A1 (en) * 2000-01-11 2006-05-26 Maxygen Inc Integrated systems for diversity generation and screening
EP1255826B1 (en) * 2000-02-10 2005-09-14 Xencor Protein design automation for protein libraries
SG95640A1 (en) * 2001-02-12 2003-04-23 Council Scient Ind Res Safe, eco-friendly, health protective herbal colours and aroma useful for cosmaceutical applications
JP4932444B2 (en) * 2006-11-13 2012-05-16 Ｎｅｃソフト株式会社 Nucleic acid higher order structure prediction apparatus, nucleic acid higher order structure prediction method, program, and recording medium
JP5445354B2 (en) * 2010-06-28 2014-03-19 Ｔｄｋ株式会社 Aptamer selection method

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5605793A (en) * 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
JPH1014578A (en) * 1996-07-01 1998-01-20 Mitsubishi Chem Corp Construction of transgenic mutant gene DNA
CA2262116A1 (en) * 1996-07-26 1998-02-05 Yamanouchi Pharmaceutical Co., Ltd. Method for searching for peptides and process for producing compounds
JPH1066577A (en) * 1996-08-07 1998-03-10 Novo Nordisk As Nucleic acid pool and its production

Also Published As

Publication number Publication date
AU8886798A (en) 1999-03-22
WO1999011818A1 (en) 1999-03-11

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