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WO1999066055A3 - Sequences de regulation inductibles par des pathogenes de vegetaux, liees de maniere operationnelle a des genes du cycle cellulaire, et utilisation desdites sequences - Google Patents

Sequences de regulation inductibles par des pathogenes de vegetaux, liees de maniere operationnelle a des genes du cycle cellulaire, et utilisation desdites sequences Download PDF

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Publication number
WO1999066055A3
WO1999066055A3 PCT/EP1999/004139 EP9904139W WO9966055A3 WO 1999066055 A3 WO1999066055 A3 WO 1999066055A3 EP 9904139 W EP9904139 W EP 9904139W WO 9966055 A3 WO9966055 A3 WO 9966055A3
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WO
WIPO (PCT)
Prior art keywords
cell cycle
operably linked
control sequences
plant pathogen
inducible control
Prior art date
Application number
PCT/EP1999/004139
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English (en)
Other versions
WO1999066055A2 (fr
Inventor
Godlieve Gheysen
Vladimir Mironov
Dirk Gustaaf Inze
Franky Raymond Gerard Terras
Camp Wim Van
Molinero Ana Isabel Sanz
Original Assignee
Cropdesign Nv
Godlieve Gheysen
Vladimir Mironov
Dirk Gustaaf Inze
Franky Raymond Gerard Terras
Camp Wim Van
Molinero Ana Isabel Sanz
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cropdesign Nv, Godlieve Gheysen, Vladimir Mironov, Dirk Gustaaf Inze, Franky Raymond Gerard Terras, Camp Wim Van, Molinero Ana Isabel Sanz filed Critical Cropdesign Nv
Priority to EP99931073A priority Critical patent/EP1088091A2/fr
Priority to AU47725/99A priority patent/AU757829B2/en
Priority to CA002330550A priority patent/CA2330550A1/fr
Publication of WO1999066055A2 publication Critical patent/WO1999066055A2/fr
Publication of WO1999066055A3 publication Critical patent/WO1999066055A3/fr

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    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/12Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237Externally regulated expression systems
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237Externally regulated expression systems
    • C12N15/8239Externally regulated expression systems pathogen inducible
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8282Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for fungal resistance
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    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8283Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for virus resistance
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8285Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for nematode resistance
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146Genetically Modified [GMO] plants, e.g. transgenic plants

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  • Health & Medical Sciences (AREA)
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  • Wood Science & Technology (AREA)
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  • Plant Pathology (AREA)
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  • Cell Biology (AREA)
  • Communicable Diseases (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

La perte de rendement due à une infestation par des pathogènes, par ex. des géminivirus ou des nématodes, constitue un problème majeur pour les plantes ou en particulier pour les cultures. Des impératifs en matière d'environnement restreignent l'utilisation de composés toxiques pour lutter contre lesdits agents infectieux. La présente invention concerne généralement des séquences de régulation inductibles par des pathogènes de plantes, telles que des promoteurs qui sont liés de manière opérationnelle à des gènes du cycle cellulaire et qui sont -en combinaison- capables de modifier le cycle cellulaire ou la division cellulaire d'une cellule végétale.
PCT/EP1999/004139 1998-06-15 1999-06-15 Sequences de regulation inductibles par des pathogenes de vegetaux, liees de maniere operationnelle a des genes du cycle cellulaire, et utilisation desdites sequences WO1999066055A2 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP99931073A EP1088091A2 (fr) 1998-06-15 1999-06-15 Sequences de regulation inductibles par des pathogenes de vegetaux, liees de maniere operationnelle a des genes du cycle cellulaire, et utilisation desdites sequences
AU47725/99A AU757829B2 (en) 1998-06-15 1999-06-15 Plant pathogen inducible control sequences operably linked to cell cycle genes and the uses thereof
CA002330550A CA2330550A1 (fr) 1998-06-15 1999-06-15 Sequences de regulation inductibles par des pathogenes de vegetaux, liees de maniere operationnelle a des genes du cycle cellulaire, et utilisation desdites sequences

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP98202012.5 1998-06-15
EP98202012 1998-06-15

Publications (2)

Publication Number Publication Date
WO1999066055A2 WO1999066055A2 (fr) 1999-12-23
WO1999066055A3 true WO1999066055A3 (fr) 2000-03-02

Family

ID=8233816

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1999/004139 WO1999066055A2 (fr) 1998-06-15 1999-06-15 Sequences de regulation inductibles par des pathogenes de vegetaux, liees de maniere operationnelle a des genes du cycle cellulaire, et utilisation desdites sequences

Country Status (4)

Country Link
EP (1) EP1088091A2 (fr)
AU (1) AU757829B2 (fr)
CA (1) CA2330550A1 (fr)
WO (1) WO1999066055A2 (fr)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6284947B1 (en) 1999-02-25 2001-09-04 Pioneer Hi-Bred International, Inc. Methods of using viral replicase polynucleotides and polypeptides
WO2000037645A2 (fr) * 1998-12-23 2000-06-29 Pioneer Hi-Bred International, Inc. Acides nucleiques intervenant dans le cycle cellulaire, polypeptides et leurs utilisations
CA2263067A1 (fr) * 1999-02-26 2000-08-26 The Australian National University Methode pour modifier la morphologie, la biochimie et la physiologie des plantes
AU2001277459A1 (en) * 2000-06-09 2001-12-17 Institut Fur Pflanzengenetik Und Kulturpflanzen Forschung Hero resistance gene conferring resistance to nematodes
EP1301532B1 (fr) 2000-07-14 2011-02-02 CropDesign N.V. Inhibiteurs de kinase a dependance de cycline dans des plantes
MX373109B (es) * 2013-04-24 2020-03-25 Centro De Investig Y De Estudios Avanzados Del I P N Métodos para obtener plantas genéticamente modificadas resistentes a bacterias fitopatógenas que crecen en sus tejidos vasculares.

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992009685A1 (fr) * 1990-11-29 1992-06-11 The Australian National University Procede de regulation de la proliferation et de la croissance des cellules vegetales
WO1992021757A1 (fr) * 1991-05-30 1992-12-10 Plant Genetic Systems, N.V. Promoteurs destines aux plantes et reagissant aux nematodes
WO1994016077A1 (fr) * 1993-01-08 1994-07-21 Ciba-Geigy Ag Procede pour induire une resistance aux maladies dans des plantes
WO1995003690A1 (fr) * 1993-08-02 1995-02-09 Virginia Tech Intellectual Properties, Inc. Systeme d'expression du promoteur hmg2 et production post-recolte de produits geniques chez des plantes et dans des cultures vegetales
WO1995032288A1 (fr) * 1994-05-25 1995-11-30 The Regents Of The University Of California Genes induits par des nematodes dans la tomate
WO1997047745A1 (fr) * 1996-06-13 1997-12-18 Consejo Superior De Investigaciones Cientificas Proteines vegetales associees au retinoblastome
WO1998003631A1 (fr) * 1996-07-18 1998-01-29 The Salk Institute For Biological Studies Procede d'accroissement de la croissance et du rendement de plantes

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992009685A1 (fr) * 1990-11-29 1992-06-11 The Australian National University Procede de regulation de la proliferation et de la croissance des cellules vegetales
WO1992021757A1 (fr) * 1991-05-30 1992-12-10 Plant Genetic Systems, N.V. Promoteurs destines aux plantes et reagissant aux nematodes
WO1994016077A1 (fr) * 1993-01-08 1994-07-21 Ciba-Geigy Ag Procede pour induire une resistance aux maladies dans des plantes
WO1995003690A1 (fr) * 1993-08-02 1995-02-09 Virginia Tech Intellectual Properties, Inc. Systeme d'expression du promoteur hmg2 et production post-recolte de produits geniques chez des plantes et dans des cultures vegetales
WO1995032288A1 (fr) * 1994-05-25 1995-11-30 The Regents Of The University Of California Genes induits par des nematodes dans la tomate
WO1997047745A1 (fr) * 1996-06-13 1997-12-18 Consejo Superior De Investigaciones Cientificas Proteines vegetales associees au retinoblastome
WO1998003631A1 (fr) * 1996-07-18 1998-01-29 The Salk Institute For Biological Studies Procede d'accroissement de la croissance et du rendement de plantes

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
BARTHELS N ET AL: "REGULATORY SEQUENCES OF ARABIDOPSIS DRIVE REPORTER GENE EXPRESSION IN NEMATODE FEEDING STRUCTURES", PLANT CELL,US,AMERICAN SOCIETY OF PLANT PHYSIOLOGISTS, ROCKVILLE, MD, vol. 9, pages 2119-2134, XP002057587, ISSN: 1040-4651 *
DE ALMEIDA ENGLER, J., ET AL.: "molecular markers and cell cycle inhibitors show the importance of cell cycle progression in nematode-induced galls and syncytia", THE PLANT CELL, vol. 11, May 1999 (1999-05-01), pages 793 - 807, XP002126398 *
HEMERLY A ET AL: "DOMINANT NEGATIVE MUTANTS OF THE CDC2 KINASE UNCOUPLE CELL DIVISIONFROM ITERATIVE PLANT DEVELOPMENT", EMBO JOURNAL, vol. 14, no. 16, 1995, pages 3925 - 3936, XP002045514 *
NIEBEL,A., ET AL.: "induction of cdc2a and cyc1at expression in Arabidopsis thaliana during early phases of nematode-induced feeding cell formation", THE PLANT JOURNAL, vol. 10, no. 6, 1996, pages 1037 - 1043, XP002086054 *

Also Published As

Publication number Publication date
CA2330550A1 (fr) 1999-12-23
AU4772599A (en) 2000-01-05
AU757829B2 (en) 2003-03-06
WO1999066055A2 (fr) 1999-12-23
EP1088091A2 (fr) 2001-04-04

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