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WO1999066055A3 - Plant pathogen inducible control sequences operably linked to cell cycle genes and the uses thereof - Google Patents

Plant pathogen inducible control sequences operably linked to cell cycle genes and the uses thereof Download PDF

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Publication number
WO1999066055A3
WO1999066055A3 PCT/EP1999/004139 EP9904139W WO9966055A3 WO 1999066055 A3 WO1999066055 A3 WO 1999066055A3 EP 9904139 W EP9904139 W EP 9904139W WO 9966055 A3 WO9966055 A3 WO 9966055A3
Authority
WO
WIPO (PCT)
Prior art keywords
cell cycle
operably linked
control sequences
plant pathogen
inducible control
Prior art date
Application number
PCT/EP1999/004139
Other languages
French (fr)
Other versions
WO1999066055A2 (en
Inventor
Godlieve Gheysen
Vladimir Mironov
Dirk Gustaaf Inze
Franky Raymond Gerard Terras
Camp Wim Van
Molinero Ana Isabel Sanz
Original Assignee
Cropdesign Nv
Godlieve Gheysen
Vladimir Mironov
Dirk Gustaaf Inze
Franky Raymond Gerard Terras
Camp Wim Van
Molinero Ana Isabel Sanz
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cropdesign Nv, Godlieve Gheysen, Vladimir Mironov, Dirk Gustaaf Inze, Franky Raymond Gerard Terras, Camp Wim Van, Molinero Ana Isabel Sanz filed Critical Cropdesign Nv
Priority to EP99931073A priority Critical patent/EP1088091A2/en
Priority to AU47725/99A priority patent/AU757829B2/en
Priority to CA002330550A priority patent/CA2330550A1/en
Publication of WO1999066055A2 publication Critical patent/WO1999066055A2/en
Publication of WO1999066055A3 publication Critical patent/WO1999066055A3/en

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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237—Externally regulated expression systems
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237—Externally regulated expression systems
    • C12N15/8239—Externally regulated expression systems pathogen inducible
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8282—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for fungal resistance
    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8283—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for virus resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8285—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for nematode resistance
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Communicable Diseases (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

Yield loss due to pathogenic, e.g., geminiviral or nematode, infection is a major problem in the cultivation of plants or, in particular, crops as such. Environmental concerns restrict the use of toxic compounds to combat said infectious agents. The current invention is generally directed to plant pathogen inducible control sequences such as promoters which are operably linked to cell cycle genes and which are - in combination - capable of modifying the cell cycle or cell division of a plant cell.
PCT/EP1999/004139 1998-06-15 1999-06-15 Plant pathogen inducible control sequences operably linked to cell cycle genes and the uses thereof WO1999066055A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP99931073A EP1088091A2 (en) 1998-06-15 1999-06-15 Plant pathogen inducible control sequences operably linked to cell cycle genes and the uses thereof
AU47725/99A AU757829B2 (en) 1998-06-15 1999-06-15 Plant pathogen inducible control sequences operably linked to cell cycle genes and the uses thereof
CA002330550A CA2330550A1 (en) 1998-06-15 1999-06-15 Plant pathogen inducible control sequences operably linked to cell cycle genes and the uses thereof

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP98202012.5 1998-06-15
EP98202012 1998-06-15

Publications (2)

Publication Number Publication Date
WO1999066055A2 WO1999066055A2 (en) 1999-12-23
WO1999066055A3 true WO1999066055A3 (en) 2000-03-02

Family

ID=8233816

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1999/004139 WO1999066055A2 (en) 1998-06-15 1999-06-15 Plant pathogen inducible control sequences operably linked to cell cycle genes and the uses thereof

Country Status (4)

Country Link
EP (1) EP1088091A2 (en)
AU (1) AU757829B2 (en)
CA (1) CA2330550A1 (en)
WO (1) WO1999066055A2 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6284947B1 (en) 1999-02-25 2001-09-04 Pioneer Hi-Bred International, Inc. Methods of using viral replicase polynucleotides and polypeptides
WO2000037645A2 (en) * 1998-12-23 2000-06-29 Pioneer Hi-Bred International, Inc. Cell cycle nucleic acids, polypeptides and uses thereof
CA2263067A1 (en) * 1999-02-26 2000-08-26 The Australian National University Method of modifying plant morphology, biochemistry and physiology
AU2001277459A1 (en) * 2000-06-09 2001-12-17 Institut Fur Pflanzengenetik Und Kulturpflanzen Forschung Hero resistance gene conferring resistance to nematodes
EP1301532B1 (en) 2000-07-14 2011-02-02 CropDesign N.V. Plant cyclin-dependent kinase inhibitors
MX373109B (en) * 2013-04-24 2020-03-25 Centro De Investig Y De Estudios Avanzados Del I P N METHODS FOR OBTAINING GENETICALLY MODIFIED PLANTS RESISTANT TO PHYTOPATHOGENIC BACTERIA GROWING IN THEIR VASCULAR TISSUES.

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992009685A1 (en) * 1990-11-29 1992-06-11 The Australian National University Control of plant cell proliferation and growth
WO1992021757A1 (en) * 1991-05-30 1992-12-10 Plant Genetic Systems, N.V. Nematode-responsive plant promoters
WO1994016077A1 (en) * 1993-01-08 1994-07-21 Ciba-Geigy Ag Method for breeding disease resistance into plants
WO1995003690A1 (en) * 1993-08-02 1995-02-09 Virginia Tech Intellectual Properties, Inc. Hmg2 promoter expression system and post-harvest production of gene products in plants and plant cell cultures
WO1995032288A1 (en) * 1994-05-25 1995-11-30 The Regents Of The University Of California Nematode-induced genes in tomato
WO1997047745A1 (en) * 1996-06-13 1997-12-18 Consejo Superior De Investigaciones Cientificas Plant retinoblastoma-associated proteins
WO1998003631A1 (en) * 1996-07-18 1998-01-29 The Salk Institute For Biological Studies Method of increasing growth and yield in plants

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992009685A1 (en) * 1990-11-29 1992-06-11 The Australian National University Control of plant cell proliferation and growth
WO1992021757A1 (en) * 1991-05-30 1992-12-10 Plant Genetic Systems, N.V. Nematode-responsive plant promoters
WO1994016077A1 (en) * 1993-01-08 1994-07-21 Ciba-Geigy Ag Method for breeding disease resistance into plants
WO1995003690A1 (en) * 1993-08-02 1995-02-09 Virginia Tech Intellectual Properties, Inc. Hmg2 promoter expression system and post-harvest production of gene products in plants and plant cell cultures
WO1995032288A1 (en) * 1994-05-25 1995-11-30 The Regents Of The University Of California Nematode-induced genes in tomato
WO1997047745A1 (en) * 1996-06-13 1997-12-18 Consejo Superior De Investigaciones Cientificas Plant retinoblastoma-associated proteins
WO1998003631A1 (en) * 1996-07-18 1998-01-29 The Salk Institute For Biological Studies Method of increasing growth and yield in plants

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
BARTHELS N ET AL: "REGULATORY SEQUENCES OF ARABIDOPSIS DRIVE REPORTER GENE EXPRESSION IN NEMATODE FEEDING STRUCTURES", PLANT CELL,US,AMERICAN SOCIETY OF PLANT PHYSIOLOGISTS, ROCKVILLE, MD, vol. 9, pages 2119-2134, XP002057587, ISSN: 1040-4651 *
DE ALMEIDA ENGLER, J., ET AL.: "molecular markers and cell cycle inhibitors show the importance of cell cycle progression in nematode-induced galls and syncytia", THE PLANT CELL, vol. 11, May 1999 (1999-05-01), pages 793 - 807, XP002126398 *
HEMERLY A ET AL: "DOMINANT NEGATIVE MUTANTS OF THE CDC2 KINASE UNCOUPLE CELL DIVISIONFROM ITERATIVE PLANT DEVELOPMENT", EMBO JOURNAL, vol. 14, no. 16, 1995, pages 3925 - 3936, XP002045514 *
NIEBEL,A., ET AL.: "induction of cdc2a and cyc1at expression in Arabidopsis thaliana during early phases of nematode-induced feeding cell formation", THE PLANT JOURNAL, vol. 10, no. 6, 1996, pages 1037 - 1043, XP002086054 *

Also Published As

Publication number Publication date
CA2330550A1 (en) 1999-12-23
AU4772599A (en) 2000-01-05
AU757829B2 (en) 2003-03-06
WO1999066055A2 (en) 1999-12-23
EP1088091A2 (en) 2001-04-04

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