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WO1999059810A2 - Films microstructures - Google Patents

Films microstructures Download PDF

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Publication number
WO1999059810A2
WO1999059810A2 PCT/EP1999/002971 EP9902971W WO9959810A2 WO 1999059810 A2 WO1999059810 A2 WO 1999059810A2 EP 9902971 W EP9902971 W EP 9902971W WO 9959810 A2 WO9959810 A2 WO 9959810A2
Authority
WO
WIPO (PCT)
Prior art keywords
films
film
cover layers
foils
channel
Prior art date
Application number
PCT/EP1999/002971
Other languages
German (de)
English (en)
Other versions
WO1999059810A3 (fr
Inventor
Frank Krause
Heinz Linder
Franz Schmitt
Georg Wiessmeier
Hansjörg Dürr
Karsten Dierksen
Simone Greven
Klaus Heidenreich
Peter Jähn
Jürgen Klein
Original Assignee
Bayer Aktiengesellschaft
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to AU40358/99A priority Critical patent/AU4035899A/en
Application filed by Bayer Aktiengesellschaft filed Critical Bayer Aktiengesellschaft
Priority to JP2000549457A priority patent/JP2002515351A/ja
Priority to EP99923506A priority patent/EP1079928A2/fr
Publication of WO1999059810A2 publication Critical patent/WO1999059810A2/fr
Publication of WO1999059810A3 publication Critical patent/WO1999059810A3/fr

Links

Classifications

    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F28—HEAT EXCHANGE IN GENERAL
    • F28D—HEAT-EXCHANGE APPARATUS, NOT PROVIDED FOR IN ANOTHER SUBCLASS, IN WHICH THE HEAT-EXCHANGE MEDIA DO NOT COME INTO DIRECT CONTACT
    • F28D9/00—Heat-exchange apparatus having stationary plate-like or laminated conduit assemblies for both heat-exchange media, the media being in contact with different sides of a conduit wall
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B32—LAYERED PRODUCTS
    • B32B—LAYERED PRODUCTS, i.e. PRODUCTS BUILT-UP OF STRATA OF FLAT OR NON-FLAT, e.g. CELLULAR OR HONEYCOMB, FORM
    • B32B27/00—Layered products comprising a layer of synthetic resin
    • B32B27/06—Layered products comprising a layer of synthetic resin as the main or only constituent of a layer, which is next to another layer of the same or of a different material
    • B32B27/08—Layered products comprising a layer of synthetic resin as the main or only constituent of a layer, which is next to another layer of the same or of a different material of synthetic resin
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0689—Sealing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/12—Specific details about manufacturing devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0887—Laminated structure
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F28—HEAT EXCHANGE IN GENERAL
    • F28F—DETAILS OF HEAT-EXCHANGE AND HEAT-TRANSFER APPARATUS, OF GENERAL APPLICATION
    • F28F2260/00—Heat exchangers or heat exchange elements having special size, e.g. microstructures
    • F28F2260/02—Heat exchangers or heat exchange elements having special size, e.g. microstructures having microchannels
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F28—HEAT EXCHANGE IN GENERAL
    • F28F—DETAILS OF HEAT-EXCHANGE AND HEAT-TRANSFER APPARATUS, OF GENERAL APPLICATION
    • F28F2275/00—Fastening; Joining
    • F28F2275/06—Fastening; Joining by welding
    • F28F2275/065—Fastening; Joining by welding by ultrasonic or vibration welding

Definitions

  • the invention relates to a module made of polymer materials which contains any microchannel systems and a method for producing such a module.
  • a microchannel is a channel that has dimensions of less than 1 mm in one or two spatial directions (width and depth) (micrometer range). In another spatial direction (length) it has a dimension that corresponds to a multiple of the dimension in the spatial direction with the smallest dimension.
  • a module is a carrier that contains one or more microchannel systems that can be filled and emptied through access and, if necessary, other elements such as Contains membranes.
  • Modules with micro-channel systems are used in a variety of technical areas. They are used as mixers, heat exchangers, reactors, microsensors in process equipment and as miniaturized analysis systems, e.g. used in molecular diagnostics.
  • the miniaturization of assemblies is advantageous because processes can run very quickly due to the small distances in the microchannel systems.
  • the large ratio of surface to volume promotes efficient heat exchange and enables targeted, fast temperature control. Due to their small size, a large number of modules with microchannel systems can be used in parallel and lead to a high throughput.
  • the mass transport through the channels can be controlled in a targeted manner by means of electrical voltages which are applied to the channels.
  • the pressure must be set so that the channels in the silicone are sealed on the one hand and not closed on the other.
  • microstructures by laser ablation of polymeric or ceramic substrates e.g. proposed with an excimer laser
  • Microstructured stack structures are preferably produced from metallic materials as highly efficient heat exchangers.
  • the structures are either machined or formed using chemical etching processes (US Pat. No. 4,516,632).
  • the individual microchannels are not used as individual process units, but as integral parts of a macroscopic module.
  • Microchannels primarily serve to enlarge the surface (B. Sunden et al. Adv. Eng. Heat Transf., Proc. Balt. Heat Transf. Conf., 1995).
  • the requirements for a module with microchannels are varied and vary depending on the area of application.
  • the general rule is that the channels in the modules should be 10-100 ⁇ m deep and 10 ⁇ m to 5 mm wide. They can be of any length.
  • the channels must continue to be connected to sample, reagent and buffer reservoirs in order to fill and empty them.
  • the channel structures must be able to be sealed and they must ensure efficient heat supply and heat dissipation. Efficient heat dissipation is particularly necessary to dissipate the Joule heat that arises when electrical voltages are applied to the microchannels and electrical currents flow.
  • the channel structures must be electrically insulated so that electrolytic current transport can take place in the channels.
  • Microchannels be accessible for optical detection.
  • Biocompatibility means that, for example due to the corresponding surface properties, no proteins accumulate in the channels.
  • the microstructured modules should be able to be produced in large numbers at low cost and should be easy to dispose of.
  • the module with microchannels consists of a polymer film, the thickness of which corresponds to the desired channel depth with cutouts in the form of the desired microchannel structure.
  • An upper and a lower cover layer seal this microstructured film on both sides.
  • the cover layers are planar on the side facing the structured film and form the top and bottom of the channels. At least one of the two cover layers has at least two accesses to at least one microchannel in the microstructured film.
  • the modules can also contain a plurality of micro-structured films stacked one above the other between the two cover layers.
  • the microchannels are then in different planes and have overlap areas seen perpendicular to the film plane. These allow the transition from one level to the next. In this way, arbitrarily linked, three-dimensional channel structures can be built.
  • Crystalline or semi-crystalline plastics are suitable as the polymer material for the films.
  • Transparent plastics such as polycarbonate or polymethyl methacrylate are particularly suitable.
  • Films can also be used which have a strong absorption in certain optical or infrared wavelength ranges.
  • a module can contain foils made of different materials.
  • Each film can be made up of different layers.
  • the individual layers can perform various functions, such as adhesive function or sealing functions through elastic deformability. Individual layers can also have different absorption spectra.
  • a film e.g. a non-absorbent core can be framed by absorbent cover layers.
  • the surface properties of the channels with regard to adsorption or surface potential can be specifically adapted to an application by coating the cover layers forming the channel walls or the films.
  • Coatings are polyacrylamide, polyvinyl alcohol, oligomeric alkylamines, nonionic detergents such as polyoxyethylene ether and polymeric sugars such as hydroxypropylmethyl cellulose or methyl cellulose.
  • the depth of the channels corresponds to the film thickness or its multiple if several films of the same structure are stacked on top of one another. Deeper channels allow higher throughput.
  • the microstructured films have a thickness of 10 to 1000 ⁇ m, preferably 10 to 100 ⁇ m. Film thicknesses of 25 to 75 ⁇ m are particularly suitable.
  • the length of the channels in the microstructured film is preferably 1 mm to 100 mm, particularly preferably 5 to 50 mm.
  • the channel width can at most correspond to the width of the film minus a sealing edge. It is preferably 1 ⁇ m to 5 mm and can be constant or vary over the channel length.
  • the channels can assume any orientation to one another and to limit the film.
  • channels with a rectangular basic shape channels with curved sides can be realized.
  • vertical channels in which the cutouts lie one above the other or diagonal channels in which the cutouts are offset one above the other can also be produced.
  • the upper and lower cover layers serve to seal the channel structure of the film in between.
  • the cover layers can be formed by foils with a thickness of 50 to 500 ⁇ m or by plates with a thickness greater than 500 ⁇ m. At least one of the cover layers has at least two accesses to at least one microchannel. Corresponding openings are present in one or both cover layers.
  • the cover layers can be transparent.
  • Sample, reagent and / or buffer vessels are attached to the outside of at least one cover layer above the accesses to the microchannels below.
  • the channels are filled via the sample, reagent and buffer vessels. Pressure differences or electrical potentials can also be generated via the sample, reagent and buffer vessels in order to effect mass transport in the channels.
  • the sample, reagent and buffer vessels can also be incorporated into the outer surface of the plates as a macro structure when using planar plates.
  • Membranes can be installed in the module.
  • a membrane that works according to the size exclusion principle is often suitable for the separation of macromolecules, particles, bacteria, cells or viruses.
  • the range of membranes ranges from a molecular weight of 3000 daltons for small proteins or nucleotides, to size exclusion ranges in the lower nm range for large nucleic acids and viruses, to 0.45 ⁇ m for cells.
  • the membranes are microstructured polymers, preferably polyether sulfone (PES), polyester, fleece-based acrylic polymer, polytetrafluoroethylene (PTFE), polysulfone, polypropylene (PP), glass fiber, nylon or polycarbonate.
  • PTFE polytetrafluoroethylene
  • PP polypropylene
  • glass fiber nylon or polycarbonate.
  • ion exchange membranes and adsorption phases can be used.
  • the choice of Membrane depends on the type of molecule desired and is micro-structure independent.
  • the membranes can be inserted between a cover layer and a microstructured film and / or between two films. They can also be attached to the outside of the cover layers between the cover layer and the sample, reagent or buffer vessel.
  • the membranes can be introduced into the module as an additional layer. If the membrane covers the entire surface, its membrane properties only function in the areas through which it flows. However, it can also have membrane properties only at the overlap areas of the channels.
  • Membranes can also be in the form of pieces which only cover the channel area through which flow occurs at the overlap areas of the channel structures between two foils or in the
  • Transitional area between the entrances of the cover layer and the underlying channels is Another possibility.
  • the membranes are located directly in the accesses to the microchannels in the cover layer.
  • the supply or removal of heat on the module can take place via the cover layers by contact, air or liquid cooling of the cover layers.
  • a particularly intensive heat exchange can be achieved using a film that has many parallel microchannels through which a liquid or gas flow can be conducted for heat exchange.
  • a method for producing a module from polymer materials with a microchannel system comprises structuring one or more films with the microchannels and connecting the one film with the two cover layers or connecting several stacked films with one another and with the two cover layers so that the microchannels are sealed.
  • the foils can be coated before processing or before assembling the module.
  • Membranes can also be inserted into the module.
  • the foils can be microstructured by etching, laser ablation or punching.
  • the film to be structured is fixed on a dimensionally stable and elastic base.
  • the cutting tool and the holding unit of the film to be cut can be moved against each other in all three spatial directions.
  • the film is fastened on a rotatable roller in a pretensioned state.
  • elastic films are pretensioned parallel to the cutting line or across the cutting line in order to optimize the cutting process.
  • the degree of preload depends on the modulus of elasticity of the film to be cut.
  • the cutting tool can be fixed or arranged as a rotating circular knife.
  • the circular knife exerts a cutting rolling movement, whereby the film is fixed under the knife and is not deformed. This is advantageous for plastic films with a low modulus of elasticity.
  • a fixed cutting tool is advantageous for cutting exact contours from one material.
  • a channel can advantageously be cut along its edges with two knives arranged in parallel, the distance between the knives corresponding to the channel width. This procedure provides exact cut surfaces and should be selected in particular if the required channel widths are less than 500 ⁇ m or if the cut is not made at a 90 ° angle to the film surface, so that the channel cross section of deviates from the rectangular shape.
  • the parallel cut stabilizes the film to be cut and prevents the film from moving.
  • cover layers are solid plates, they can be produced by injection molding or with other common manufacturing processes for structures made of polymer materials. Sample and buffer vessels can be made in one piece with the cover plate.
  • microstructured foils can be connected to one another and to the cover layers to form a module by printing, gluing or welding.
  • a tight connection by pressure can only be achieved with solid panels as cover layers.
  • the pressure is exerted on the two cover plates from the outside. They are pressed against each other and on the foils in between. This seals the channels of the microstructured foils.
  • Cover plates show microscopic bumps on the surfaces, which are embossed in the intervening foils and can lead to leakage currents.
  • the use of particularly elastic films such as films made of thermoplastic polyurethane (TPU) or films coated with an adhesive-like leveling compound can prevent the leaks.
  • TPU thermoplastic polyurethane
  • the elastic film and the leveling compound adapt to the unevenness of the surface and seal it.
  • the strength of the pressure on the film in between depends on the elastic properties of the film material used. A pressure of 5 to 50%, a particularly advantageous 5 to 25% of the pre-tensioning of the film is favorable O 99/59810. ⁇ Q. PCT / EP99 / 02971
  • Film thickness causes.
  • the preload is measured by changing the length of the film compared to the untensioned state.
  • the pressure on the cover plates of the module is maintained by detachable or non-detachable connections.
  • Detachable connections are screws, clips or clamping elements.
  • Inseparable connections are made by riveting or welding the two cover plates on their outer edges. When welding, the one or more foils are passively fixed between the cover plates.
  • the foils and cover layers can be tightly connected to one another by gluing.
  • the adhesive can be self-curing, thermosetting or photo-curing.
  • Unreleasable connections are particularly advantageous, which are created by welding the foils and cover layers directly at the edges of the channels.
  • cover layers do not have to be dimensionally stable. They can also be slides.
  • the desired area is melted by one-sided heating with direct contact.
  • heating element welding involves thermal contact on both sides.
  • Infrared beam welding can be carried out on one side or on both sides.
  • at least one of the foils must have an infrared-absorbing layer.
  • At least one of the foils must have a layer that absorbs the laser light.
  • radiographic welding the entire sorbent layer melted. With butt welding, only the surface is melted. Butt welding can be carried out on one side or on both sides.
  • the energy density required for welding is generated by ultrasonic waves.
  • the use of energy directors can be dispensed with using conventional ultrasonic welding devices, since the amount of energy per material volume is sufficient even without a measure for bundling the energy.
  • an alternating electric field of high frequency is radiated. Part of the energy of the alternating electrical field is converted into heat in the plastic.
  • the module made of polymer materials is welded when the dielectric loss factor tan ⁇ of the polymer is greater than 0.01, that is to say a sufficiently high proportion of the energy in the field is drawn off and converted into heat.
  • the film By using a microstructured film that absorbs all or in individual layers and transparent cover layers, the film can be selectively melted and permanently connected to the cover layers.
  • microchannel structures are not absorbent, so that the energy can be radiated in over a large area and the contour of the channels does not have to be followed exactly.
  • the surfaces of the films that contribute to the formation of a channel structure and also the channel-side surfaces of the cover layer can be coated before cutting or before assembly in order to change surface properties such as biocompatibility and surface potential.
  • Membranes are fixed together with the connection of the foils and cover layers by pressing, gluing or welding. If individual membrane pieces are introduced in the area of an access to a channel between a cover layer and a film or between two films, the membrane must face the direction in which the channel continues with the cover layer or with the film that does not support the channel • * 0- ⁇ t ",) can be connected.
  • the modules according to the invention with microchannels are distinguished on the one hand by the construction from simple standard materials (polymer films).
  • Polymer materials such as polycarbonate or polymethyl methacrylate can be used for a large number of applications because they combine the properties of inertness, biocompatibility, electrical insulation and transparency.
  • the manufacturing process for the modules according to the invention is simple and flexible. In comparison to the known silicone casting processes and micro injection molding processes, no original molds are produced, which then only result in one embodiment of the microchannel structure. Instead, the foils can be simply structured using the known methods of etching, laser ablation, punching or particularly advantageously using the cutting method according to the invention. At the
  • Cutting is done with simple cutting tools such as Razors cut any structure.
  • connection of the foils to the module in particular by means of the proposed laser and infrared welding processes, can also be carried out quickly and easily.
  • the modules according to the invention are therefore suitable for automated mass production and can also be produced inexpensively because of the inexpensive materials. Polymer materials are also easy to dispose of. It is therefore worthwhile to manufacture them as single-use modules for single use. Single-use modules have the advantage, particularly in the medical field, that they do not carry germs and contaminants can come, and their use therefore allows more precise analyzes and increases the safety of staff and patients.
  • the heat exchange is even more efficient than with conventional systems with microchannels due to the thin walls of the foils.
  • a module of polymeric materials containing any microchannel system is suitable for many types of applications for microchannel structures. Examples of applications are the chemical laboratory, polymerase chain reaction (PCR), immunodiagnostics, virus analysis and DNA analysis on the chip, implants, dosing devices and analysis with miniaturized total analysis systems or with micro-preparative sample preparation modules for DNA extraction.
  • PCR polymerase chain reaction
  • immunodiagnostics virus analysis and DNA analysis on the chip
  • implants dosing devices and analysis with miniaturized total analysis systems or with micro-preparative sample preparation modules for DNA extraction.
  • the substances in the microchannels can be detected using optical methods. Absorption and fluorescence methods can be used. Because of its high sensitivity, laser-induced fluorescence detection is often used.
  • Fig. 1 layer structure of a module with a microstructured film.
  • Fig. 2 layer structure of a module with several microstructured films.
  • Fig. 4 layer structure of a module with vessels on a cover layer.
  • Fig. 5 arrangement for cutting the films.
  • Fig. 6 module that is connected by pressure.
  • Fig. 7 Manual cutting apparatus for microstructuring polymer films.
  • Fig. 8 photocopy of the laser-welded layer structure of a module.
  • Fig. 9 electrophoretic transport in a microchannel structure.
  • the thickness of the film 2 corresponds to the channel depth.
  • the film 2 is enclosed by the lower cover layer 3 and the upper cover layer 4.
  • the cover layers form the upper and lower channel walls.
  • the cover layer 4 has the accesses 5 to the microchannel structure 1 in the film 2.
  • FIG. 3 Various possibilities for introducing the membranes 6a, 6b and 6c into the layer structure are shown in FIG. 3.
  • the membrane 6b is located above the access 5 above the cover layer 4.
  • the membrane 6a is located between the film 2 and the cover layer 4 in the area of an access 5 to the microchannels 1 the film 2.
  • the membrane 6c forms the passage in the covering layer 4 to the microchannels 1 in the film 2.
  • FIG. 4 shows a module consisting of a film 2 with microchannels 1 and the covering layers 3 and 4. On the covering layer 4 above the accesses 5 there are
  • Fig. 5 shows an arrangement for cutting the films.
  • the film 9 to be structured is located under the cutting knife 8 on the cutting table 10.
  • the film 9 to be structured is preloaded by a force 81.
  • FIG. 6 shows a module in which the structured film 13 is fixed between two dimensionally stable cover plates 14 and 15.
  • the pressure by which the microchannels in the film are sealed off by the cover plates is exerted by the screw 12 and / or the rivet 16.
  • a plurality of foils could also be stacked one above the other between the cover plates 14 and 15.
  • FIG. 7a shows a cutting apparatus for microstructuring polymer films.
  • FIG. 7b shows a microstructured film as can be produced with a cutting apparatus according to FIG. 7a.
  • the cutting apparatus consists of a rotatable roller 18 for receiving the film, a handwheel with latching positions 19, a pair of cutting knives 20 for cutting the lateral boundary of the microstructure film.
  • the two pairs of cutting blades 21 are used for cutting the longitudinal channels a and b of the microstructure.
  • the punching knife receptacle 22 can hold various punching knives for punching the transverse channel c, for punching the structure borders e and for punching the film borders f.
  • the guide 23 is used to execute the position bores designated by g.
  • Figure 8 is a photocopy of a module. In a 100 ⁇ m thick, black pigmented
  • Film 2 made of polycarbonate was cut with the manual cutting apparatus from FIG. 7 microchannels 1, 3 and 4.
  • the structured film was between two O 99/59810 _ ig _ PCT / EP99 / 02971
  • the permanently connected module has intact channel structures.
  • FIG. 9 a shows how the electrophoretic transport of fluorescence-labeled DNA takes place in the laser-welded layer module from FIG. 8.
  • 9b shows the fluorescence signal at a wavelength of 520 nm, as it is detected in the thin channel on the detector 24. Under the influence of the voltage 25a, the DNA is transported electrokinetically into the wide channel (Fig. 9a- (a)). Then the
  • a suitable piece of 100 ⁇ m thick film is first fixed on the rotatable roller 18 by means of adhesive tape.
  • the cutting length is limited to 55 mm by two locking positions of the handwheel.
  • the cut limitation after 91 mm is also done by two locking positions of the handwheel.
  • the cross-sections are punched after inserting the respective punching knives into the punching knife receptacle 22 by folding the punching knives onto the roller 18 and the film located thereon. In this way, the transverse channel c, the upper and lower limits of the microchannels d and e and the upper and lower limits of the film f are punched.
  • the adjustment bores g are produced by means of a punch 23 and two locking positions of the handwheel.
  • the microstructured film thus produced with defined external dimensions contains a narrow channel b of 100 ⁇ m in width and 55 mm in length, a wide channel a of 2 mm in width and 55 mm in length and a narrow transverse channel c of 100 ⁇ m in width and 4 mm in length.
  • the outer dimensions of the film are 20 mm wide and 91 mm long.
  • thermoplastic polyurethane TPU
  • the microchannel structure was produced in accordance with Example 1.
  • the ratio of the measured currents corresponds to the ratio of the channel cross sections of narrow and wide channels. This shows that the electrical line runs exclusively through the buffer-filled channels and the module is otherwise electrically insulating.
  • a module with a black pigmented infrared absorbing microstructured polycarbonate film of 100 ⁇ m thickness was produced.
  • the module was sealed by laser welding.
  • microchannel structure was produced in the polycarbonate film essentially in accordance with Example 1. The only difference to the method from Example 1 was that the narrow channel was cut with a width of 200 ⁇ m. The microstructured film was between two more transparent
  • the films were pressed together on a solid surface with a glass plate and scanned over a large area around the microstructure with an Nd: YAG laser beam with an effective power of 8 W.
  • the laser beam had a diameter of 2.2 mm at the location of the foils and the scanning speed was 18 mm / s.
  • the irradiated areas can be seen in FIG. 8 by the more intense black. Although only one side was irradiated, the three foils are permanently and firmly fused. As a result of the melting process, the narrow channel narrowed from 200 ⁇ m to 120 ⁇ m.
  • the module from Example 3 was glued with the side on which the hole-carrying film is located to a machined polycarbonate plate using fast-curing adhesive.
  • the plate has four buffer vessels with holes of 2 mm in diameter, which are located above the holes in the cover film of the module.
  • Tris / borate buffer which was 0.1 molar, contained 0.1% methyl cellulose and had a pH of 8.5, was introduced into the buffer reservoirs and the entire module was deaerated in vacuo. Electrodes were inserted into the buffer reservoirs. The entire module was cooled to 10 ° C using contact cooling with thermostatted metal surfaces. A voltage of 1 kV was first applied across the electrodes along the wide channel and a current of 270 ⁇ A was measured. A voltage of 1 kV was also applied along the narrow channel and a current of 25 ⁇ A was measured. This shows that the electrical line runs exclusively through the buffer-filled channels and the module is otherwise electrically insulating.
  • the module prepared in this way was tested with the experimental set-up according to FIG. 9.
  • a laser-induced fluorescence detector 24 was additionally positioned above the microstructure.
  • the laser light from an argon-ion laser with a wavelength of 488 nm was irradiated onto the module surface at an angle of 45 ° using a light guide.
  • a second light guide was positioned such that it directed light emitted at an angle of 90 ° to the module surface to the detector 24.
  • DNA that was intercalated with a fluorescence marker that was 10 micromolar was filled into a wide channel buffer reservoir. The DNA was transported along the wide channel by applying a voltage of 700 V (FIG. 9a (a)).

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  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Dispersion Chemistry (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
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  • General Health & Medical Sciences (AREA)
  • Mechanical Engineering (AREA)
  • General Engineering & Computer Science (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Lining Or Joining Of Plastics Or The Like (AREA)
  • Rigid Pipes And Flexible Pipes (AREA)
  • Heat-Exchange Devices With Radiators And Conduit Assemblies (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)
  • Laminated Bodies (AREA)

Abstract

L'invention concerne un module à base de matériaux polymères, contenant des systèmes de microcanaux de n'importe quel type, ainsi qu'un procédé permettant de produire ledit module. Le module obtenu selon l'invention avec les microcanaux, comprend un film polymère (2) dont l'épaisseur correspond à la profondeur voulue des canaux, avec des évidements (1) prenant la forme de la structure de microcanaux voulue. Une couche de protection supérieure (4) et une couche de protection inférieure (3) rendent le film microstructuré étanche sur ses deux faces. Sur le côté qui fait face au film structuré, les couches de protection sont planes et forment la face supérieure et la face inférieure des canaux. Au moins une des deux couches dispose d'au moins deux accès (5) au système de microcanaux dans le film microstructuré. L'invention concerne également un procédé de production d'un module à base de matériaux polymères avec un système de microcanaux. Ce procédé comprend la structuration d'au moins un ou plusieurs films avec des microcanaux et l'assemblage d'un film avec les deux couches de protection, de manière à rendre les microcanaux étanches.
PCT/EP1999/002971 1998-05-14 1999-05-03 Films microstructures WO1999059810A2 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
AU40358/99A AU4035899A (en) 1998-05-14 1999-04-21 Microstructured films
JP2000549457A JP2002515351A (ja) 1998-05-14 1999-05-03 ミクロ構造化されたフィルム
EP99923506A EP1079928A2 (fr) 1998-05-14 1999-05-03 Films microstructures

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE1998121627 DE19821627A1 (de) 1998-05-14 1998-05-14 Mikrostrukturierte Folien
DE19821627.0 1998-05-14

Publications (2)

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WO1999059810A2 true WO1999059810A2 (fr) 1999-11-25
WO1999059810A3 WO1999059810A3 (fr) 2000-03-16

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PCT/EP1999/002971 WO1999059810A2 (fr) 1998-05-14 1999-05-03 Films microstructures

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EP (1) EP1079928A2 (fr)
JP (1) JP2002515351A (fr)
AU (1) AU4035899A (fr)
DE (1) DE19821627A1 (fr)
WO (1) WO1999059810A2 (fr)

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JP2009063597A (ja) * 2002-04-30 2009-03-26 Arkray Inc 分析用具に対する開口形成方法
US9118042B2 (en) 2007-03-21 2015-08-25 Intelligent Energy Limited Fluidic distribution system and related methods

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DE60123817T2 (de) * 2000-07-28 2007-05-16 Honda Giken Kogyo K.K. Mehrzweck-mikrobauteil mit mikrokanälen
JP2002174610A (ja) * 2000-12-08 2002-06-21 Nec Corp バイオセンサ及びバイオセンサを用いた液体試料の測定方法
GB0126281D0 (en) * 2001-11-01 2002-01-02 Astrazeneca Ab A chemical reactor
US7279134B2 (en) * 2002-09-17 2007-10-09 Intel Corporation Microfluidic devices with porous membranes for molecular sieving, metering, and separations
JP4683538B2 (ja) * 2004-05-06 2011-05-18 セイコーインスツル株式会社 分析用マイクロチップを含む分析システムと分析方法
DE102005007707A1 (de) * 2004-09-27 2006-03-30 Powerfluid Gmbh Rekuperator, Mikrokanal-Rekuperator, Folie, Verwendung einer Folie und Verfahren zum Herstellen sowie zum Betreiben eines Rekuperators
EP1926678B1 (fr) * 2005-09-09 2013-03-20 Koninklijke Philips Electronics N.V. Procéde de fabrication de microsystéme
DE102006010956A1 (de) * 2006-03-06 2007-09-13 Directif Gmbh Ventil
KR101522418B1 (ko) * 2007-03-21 2015-05-21 소시에떼 비아이씨 유체 다기관 및 그 방법
DE102008051091B3 (de) * 2008-10-09 2010-06-10 Bleckmann Gmbh & Co. Kg Plattenwärmeübertrager und eine Anordnung aus einem solchen und einer Fluidheizung
CN103003702B (zh) * 2010-06-30 2015-04-15 美特宝思科润株式会社 微型化学芯片、其制造方法及其使用方法
JP6057166B2 (ja) * 2013-01-18 2017-01-11 大日本印刷株式会社 構造物、構造物の製造方法、及び成形品の製造方法
JP5685630B2 (ja) * 2013-07-19 2015-03-18 Ｊｆｅエンジニアリング株式会社 マイクロチップ
WO2016128843A2 (fr) 2015-01-14 2016-08-18 Pixcell Medical Technologies Ltd. Cartouche jetable pour analyse de liquide d'essai
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JP2004537033A (ja) * 2001-02-22 2004-12-09 プラント・バイオサイエンス・リミテッド 荷電化合物の単離方法
JP2009063597A (ja) * 2002-04-30 2009-03-26 Arkray Inc 分析用具に対する開口形成方法
US9118042B2 (en) 2007-03-21 2015-08-25 Intelligent Energy Limited Fluidic distribution system and related methods
US9728796B2 (en) 2007-03-21 2017-08-08 Intelligent Energy Limited Fluidic distribution system and related methods

Also Published As

Publication number Publication date
AU4035899A (en) 1999-12-06
WO1999059810A3 (fr) 2000-03-16
DE19821627A1 (de) 1999-11-18
EP1079928A2 (fr) 2001-03-07
JP2002515351A (ja) 2002-05-28

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