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WO1999053037A3 - Sequences optimisees de nucleosides codant pour des hydrolases organophosphorees, et leurs methodes d'utilisation - Google Patents

Sequences optimisees de nucleosides codant pour des hydrolases organophosphorees, et leurs methodes d'utilisation Download PDF

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Publication number
WO1999053037A3
WO1999053037A3 PCT/US1999/008202 US9908202W WO9953037A3 WO 1999053037 A3 WO1999053037 A3 WO 1999053037A3 US 9908202 W US9908202 W US 9908202W WO 9953037 A3 WO9953037 A3 WO 9953037A3
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WO
WIPO (PCT)
Prior art keywords
nucleotide sequence
methods
same
sequence encoding
expression
Prior art date
Application number
PCT/US1999/008202
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English (en)
Other versions
WO1999053037A2 (fr
Inventor
Joseph M Jilka
Original Assignee
Prodigene Inc
Joseph M Jilka
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Prodigene Inc, Joseph M Jilka filed Critical Prodigene Inc
Priority to AU40689/99A priority Critical patent/AU4068999A/en
Publication of WO1999053037A2 publication Critical patent/WO1999053037A2/fr
Publication of WO1999053037A3 publication Critical patent/WO1999053037A3/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

L'invention porte sur: une séquence de nucléotides optimisée en vue de l'expression de plantes et codant pour des hydrolases organophosphorées. L'invention porte également sur des produits d'assemblage d'expression, sur des vecteurs de transformation et sur des cellules transformées atteignant des niveaux d'expression supérieurs à ceux obtenus par expression dans des bactéries telles que le E. Coli en vue de la production à fort rendement de l'enzyme. On peut utiliser les plantes transgéniques elles-mêmes ou récolter la protéine dans lesdites plantes pour la détoxication in situ ou autre de l'environnement dans des zones contaminées par des neurotoxines organophosphorées.
PCT/US1999/008202 1998-04-15 1999-04-14 Sequences optimisees de nucleosides codant pour des hydrolases organophosphorees, et leurs methodes d'utilisation WO1999053037A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU40689/99A AU4068999A (en) 1998-04-15 1999-04-14 Optimized nucleotide sequence encoding organophosphorous hydrolase and methods of use for same

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US8184698P 1998-04-15 1998-04-15
US60/081,846 1998-04-15

Publications (2)

Publication Number Publication Date
WO1999053037A2 WO1999053037A2 (fr) 1999-10-21
WO1999053037A3 true WO1999053037A3 (fr) 1999-12-09

Family

ID=22166780

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/008202 WO1999053037A2 (fr) 1998-04-15 1999-04-14 Sequences optimisees de nucleosides codant pour des hydrolases organophosphorees, et leurs methodes d'utilisation

Country Status (2)

Country Link
AU (1) AU4068999A (fr)
WO (1) WO1999053037A2 (fr)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001071014A2 (fr) * 2000-03-17 2001-09-27 Boyce Thompson Institute For Plant Research, Inc. Expression d'acetylcholinesterase de recombinaison humaine dans des plantes transgeniques
AUPR502301A0 (en) * 2001-05-15 2001-06-07 Commonwealth Scientific And Industrial Research Organisation Phosphotriesterase from Agrobacterium radiobacter P230
US20040109853A1 (en) 2002-09-09 2004-06-10 Reactive Surfaces, Ltd. Biological active coating components, coatings, and coated surfaces
US7060501B2 (en) * 2002-12-03 2006-06-13 Applied Biotechnology Institute Methods for selecting and screening for plant cells transformed with a polynucleotide encoding an organophosphate hydrolase
CN1330760C (zh) * 2005-10-11 2007-08-08 中国农业科学院生物技术研究所 重组有机磷降解酶基因、其表达载体及重组有机磷降解酶的制备方法
EP2441834A1 (fr) * 2007-10-01 2012-04-18 Guild Associates, Inc. Biocapteurs de levure différentiellement fluorescente pour la détection et la biodégradation d'agents chimiques
US8388904B1 (en) 2008-12-22 2013-03-05 Reactive Surfaces, Ltd., Llp Equipment decontamination system and method
CN102145218A (zh) * 2010-11-24 2011-08-10 高旌 一种植物体中农药快速降解剂
AU2012286529A1 (en) 2011-07-20 2014-02-06 Commonwealth Scientific And Industrial Research Organisation Enzymes for degrading organophosphates

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990002177A1 (fr) * 1988-08-26 1990-03-08 Amgen Inc. Analogues d'hydrolase de parathion et procedes de production et de purification
EP0682115A1 (fr) * 1988-09-09 1995-11-15 Mycogen Plant Science, Inc. Gène synthétique pour une protéine-cristal insecticide
US5474925A (en) * 1991-12-19 1995-12-12 Agracetus, Inc. Immobilized proteins in cotton fiber

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990002177A1 (fr) * 1988-08-26 1990-03-08 Amgen Inc. Analogues d'hydrolase de parathion et procedes de production et de purification
EP0682115A1 (fr) * 1988-09-09 1995-11-15 Mycogen Plant Science, Inc. Gène synthétique pour une protéine-cristal insecticide
US5474925A (en) * 1991-12-19 1995-12-12 Agracetus, Inc. Immobilized proteins in cotton fiber

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
CUNNINGHAM S D ET AL: "PROMISES AND PROSPECTS OF PHYTOREMEDIATION", PLANT PHYSIOLOGY, vol. 110, 1 January 1996 (1996-01-01), pages 715 - 719, XP002051868, ISSN: 0032-0889 *
SALT D E ET AL: "PHYTOREMEDIATION: A NOVEL STRATEGY FOR THE REMOVAL OF TOXIC METALS FROM THE ENVIRONMENT USING PLANTS", BIO/TECHNOLOGY, vol. 13, no. 5, 1 May 1995 (1995-05-01), pages 468 - 474, XP000577130, ISSN: 0733-222X *
XU B ET AL.: "Enhanced Expression of a Bacterial Gene for Pesticide Degradation in a Common Soil Fungus", JOURNAL OF FERMENTATION AND BIOENGINEERING, vol. 81, no. 6, 1996, pages 473 - 481, XP002112416 *

Also Published As

Publication number Publication date
WO1999053037A2 (fr) 1999-10-21
AU4068999A (en) 1999-11-01

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