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WO1999053037A3 - Optimized nucleotide sequence encoding organophosphorous hydrolase and methods of use for same - Google Patents

Optimized nucleotide sequence encoding organophosphorous hydrolase and methods of use for same Download PDF

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Publication number
WO1999053037A3
WO1999053037A3 PCT/US1999/008202 US9908202W WO9953037A3 WO 1999053037 A3 WO1999053037 A3 WO 1999053037A3 US 9908202 W US9908202 W US 9908202W WO 9953037 A3 WO9953037 A3 WO 9953037A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleotide sequence
methods
same
sequence encoding
expression
Prior art date
Application number
PCT/US1999/008202
Other languages
French (fr)
Other versions
WO1999053037A2 (en
Inventor
Joseph M Jilka
Original Assignee
Prodigene Inc
Joseph M Jilka
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Prodigene Inc, Joseph M Jilka filed Critical Prodigene Inc
Priority to AU40689/99A priority Critical patent/AU4068999A/en
Publication of WO1999053037A2 publication Critical patent/WO1999053037A2/en
Publication of WO1999053037A3 publication Critical patent/WO1999053037A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

A nucleotide sequence is disclosed which is optimized for plant expression and which encodes organophosphorous hydrolase. Expression constructs, transformation vectors as well as transformed cells are also disclosed which achieve expression levels superior to those demonstrated by expression in bacteria such as E. coli for high throughput production of the enzyme. Transgenic plants may themselves be used or the protein may be harvested from said plants for in situ or other environmental detoxification of organophosphorous neurotoxin contaminated areas.
PCT/US1999/008202 1998-04-15 1999-04-14 Optimized nucleotide sequence encoding organophosphorous hydrolase and methods of use for same WO1999053037A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU40689/99A AU4068999A (en) 1998-04-15 1999-04-14 Optimized nucleotide sequence encoding organophosphorous hydrolase and methods of use for same

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US8184698P 1998-04-15 1998-04-15
US60/081,846 1998-04-15

Publications (2)

Publication Number Publication Date
WO1999053037A2 WO1999053037A2 (en) 1999-10-21
WO1999053037A3 true WO1999053037A3 (en) 1999-12-09

Family

ID=22166780

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/008202 WO1999053037A2 (en) 1998-04-15 1999-04-14 Optimized nucleotide sequence encoding organophosphorous hydrolase and methods of use for same

Country Status (2)

Country Link
AU (1) AU4068999A (en)
WO (1) WO1999053037A2 (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001071014A2 (en) * 2000-03-17 2001-09-27 Boyce Thompson Institute For Plant Research, Inc. Expression of recombinant human acetylcholinesterase in transgenic plants
AUPR502301A0 (en) * 2001-05-15 2001-06-07 Commonwealth Scientific And Industrial Research Organisation Phosphotriesterase from Agrobacterium radiobacter P230
US20040109853A1 (en) 2002-09-09 2004-06-10 Reactive Surfaces, Ltd. Biological active coating components, coatings, and coated surfaces
WO2004070003A2 (en) * 2002-12-03 2004-08-19 Prodigene, Inc. Methods for selecting and screening for trasformants
CN1330760C (en) * 2005-10-11 2007-08-08 中国农业科学院生物技术研究所 Recombinant organophosphate degrading enzyme gene and its expression vector and prepn process
US8927244B2 (en) 2007-10-01 2015-01-06 Guild Associates, Inc. Organophosphorus hydrolase
US8388904B1 (en) 2008-12-22 2013-03-05 Reactive Surfaces, Ltd., Llp Equipment decontamination system and method
CN102145218A (en) * 2010-11-24 2011-08-10 高旌 Rapid degradation agent for pesticide in plant
AU2012286529A1 (en) 2011-07-20 2014-02-06 Commonwealth Scientific And Industrial Research Organisation Enzymes for degrading organophosphates

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990002177A1 (en) * 1988-08-26 1990-03-08 Amgen Inc. Parathion hydrolase analogs and methods for production and purification
EP0682115A1 (en) * 1988-09-09 1995-11-15 Mycogen Plant Science, Inc. Synthetic insecticidal crystal protein gene
US5474925A (en) * 1991-12-19 1995-12-12 Agracetus, Inc. Immobilized proteins in cotton fiber

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990002177A1 (en) * 1988-08-26 1990-03-08 Amgen Inc. Parathion hydrolase analogs and methods for production and purification
EP0682115A1 (en) * 1988-09-09 1995-11-15 Mycogen Plant Science, Inc. Synthetic insecticidal crystal protein gene
US5474925A (en) * 1991-12-19 1995-12-12 Agracetus, Inc. Immobilized proteins in cotton fiber

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
CUNNINGHAM S D ET AL: "PROMISES AND PROSPECTS OF PHYTOREMEDIATION", PLANT PHYSIOLOGY, vol. 110, 1 January 1996 (1996-01-01), pages 715 - 719, XP002051868, ISSN: 0032-0889 *
SALT D E ET AL: "PHYTOREMEDIATION: A NOVEL STRATEGY FOR THE REMOVAL OF TOXIC METALS FROM THE ENVIRONMENT USING PLANTS", BIO/TECHNOLOGY, vol. 13, no. 5, 1 May 1995 (1995-05-01), pages 468 - 474, XP000577130, ISSN: 0733-222X *
XU B ET AL.: "Enhanced Expression of a Bacterial Gene for Pesticide Degradation in a Common Soil Fungus", JOURNAL OF FERMENTATION AND BIOENGINEERING, vol. 81, no. 6, 1996, pages 473 - 481, XP002112416 *

Also Published As

Publication number Publication date
AU4068999A (en) 1999-11-01
WO1999053037A2 (en) 1999-10-21

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