+

WO1999050423A3 - Genes de la voie de l'acide salicylique et leur utilisation pour induire la resistance chez les vegetaux - Google Patents

Genes de la voie de l'acide salicylique et leur utilisation pour induire la resistance chez les vegetaux Download PDF

Info

Publication number
WO1999050423A3
WO1999050423A3 PCT/EP1999/002176 EP9902176W WO9950423A3 WO 1999050423 A3 WO1999050423 A3 WO 1999050423A3 EP 9902176 W EP9902176 W EP 9902176W WO 9950423 A3 WO9950423 A3 WO 9950423A3
Authority
WO
WIPO (PCT)
Prior art keywords
plants
isochorismate
gene
gene coding
isochorismate synthase
Prior art date
Application number
PCT/EP1999/002176
Other languages
English (en)
Other versions
WO1999050423A2 (fr
Inventor
Hubertus Josephus Ma Linthorst
Robert Verpoorte
Maria Catharina Verberne
Paulo R H Moreno
Tegelen Leonardus Johannes Van
George Joseph Wullems
Anton Felix Croes
Maarten Hendrik Stuiver
Jerome Hubertina Henri Custers
Lambertus Henricus Simons
Leo Sjoerd Melchers
John Ferdinand Bol
Original Assignee
Mogen Int
Univ Leiden
Univ Nijmegen
Linthorst Hubertus J M
Robert Verpoorte
Maria Catharina Verberne
Paulo R H Moreno
Tegelen Leonardus Johannes Pet
George Joseph Wullems
Anton Felix Croes
Maarten Hendrik Stuiver
Jerome Hubertina Henri Custers
Lambertus Henricus Simons
Leo Sjoerd Melchers
John Ferdinand Bol
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mogen Int, Univ Leiden, Univ Nijmegen, Linthorst Hubertus J M, Robert Verpoorte, Maria Catharina Verberne, Paulo R H Moreno, Tegelen Leonardus Johannes Pet, George Joseph Wullems, Anton Felix Croes, Maarten Hendrik Stuiver, Jerome Hubertina Henri Custers, Lambertus Henricus Simons, Leo Sjoerd Melchers, John Ferdinand Bol filed Critical Mogen Int
Priority to CA002333433A priority Critical patent/CA2333433A1/fr
Priority to EP99917919A priority patent/EP1066389A2/fr
Priority to JP2000541311A priority patent/JP2003513608A/ja
Priority to MXPA00009573A priority patent/MXPA00009573A/es
Priority to BR9909303-0A priority patent/BR9909303A/pt
Priority to AU36025/99A priority patent/AU746787B2/en
Publication of WO1999050423A2 publication Critical patent/WO1999050423A2/fr
Publication of WO1999050423A3 publication Critical patent/WO1999050423A3/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237—Externally regulated expression systems
    • C12N15/8239—Externally regulated expression systems pathogen inducible
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8282—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for fungal resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8283—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for virus resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Communicable Diseases (AREA)
  • Virology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

L'invention concerne une technique qui permet d'induire la résistance aux pathogènes chez les végétaux, caractérisée par le fait que lesdits végétaux sont transformés avec une cassette d'expression hébergeant un gène codant pour une isochorismate synthase. Plus spécifiquement, cette technique est caractérisée par le fait que le gène codant pour ladite synthase est choisi dans un groupe comprenant entC, orfA, pchA et ICS, ce dernier étant de préférence le gène ICS de Catharantus roseus. Dans un autre mode de réalisation, l'invention concerne une technique conforme à la technique ci-dessus, caractérisée par le fait que les végétaux sont en outre transformés avec une cassette d'expression hébergeant un gène codant pour un isochorismate pyruvate lyase, de préférence situé sur la même cassette d'expression que le gène codant pour l'isochorismate synthase. Le gène codant pour l'isochorismate pyruvate lyase est de préférence choisi dans le groupe comprenant orfD et pchB. Un autre aspect de l'invention est une protéine présentant une activité isochorismate synthase, isolée à partir de Catharantus roseus. Un autre aspect de l'invention est la séquence nucléotidique comprenant la région régulatrice 5' qui naturellement régule l'expression du gène ICS chez Catharantus roseus.
PCT/EP1999/002176 1998-03-31 1999-03-25 Genes de la voie de l'acide salicylique et leur utilisation pour induire la resistance chez les vegetaux WO1999050423A2 (fr)

Priority Applications (6)

Application Number Priority Date Filing Date Title
CA002333433A CA2333433A1 (fr) 1998-03-31 1999-03-25 Genes de la voie de l'acide salicylique et leur utilisation pour induire la resistance chez les vegetaux
EP99917919A EP1066389A2 (fr) 1998-03-31 1999-03-25 Genes de la voie de l'acide salicylique et leur utilisation pour induire la resistance chez les vegetaux
JP2000541311A JP2003513608A (ja) 1998-03-31 1999-03-25 イソコリスミ酸シンターゼおよび植物における耐性の誘導のためのその使用
MXPA00009573A MXPA00009573A (es) 1998-03-31 1999-03-25 Isocorismato sintasa y su uso para la induccion de resistencia en las plantas.
BR9909303-0A BR9909303A (pt) 1998-03-31 1999-03-25 Processo para induzir resistência a patógeno em plantas, proteìna que tem a atividade de isocorismato sintase, sequência de nucleotìdeo, promotor indutìvel por patógeno, uso do mesmo, vetor, cepa de agrobacterium, células de plantas, e, plantas
AU36025/99A AU746787B2 (en) 1998-03-31 1999-03-25 Salicylic acid pathway genes and their use for the induction of resistance in plants

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US8020398P 1998-03-31 1998-03-31
US60/080,203 1998-03-31
US8062598P 1998-04-03 1998-04-03
US60/080,625 1998-04-03

Publications (2)

Publication Number Publication Date
WO1999050423A2 WO1999050423A2 (fr) 1999-10-07
WO1999050423A3 true WO1999050423A3 (fr) 1999-12-16

Family

ID=26763209

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1999/002176 WO1999050423A2 (fr) 1998-03-31 1999-03-25 Genes de la voie de l'acide salicylique et leur utilisation pour induire la resistance chez les vegetaux

Country Status (13)

Country Link
EP (1) EP1066389A2 (fr)
JP (1) JP2003513608A (fr)
CN (1) CN1295619A (fr)
AR (1) AR018172A1 (fr)
AU (1) AU746787B2 (fr)
BR (1) BR9909303A (fr)
CA (1) CA2333433A1 (fr)
CO (1) CO5050251A1 (fr)
MA (1) MA24793A1 (fr)
MX (1) MXPA00009573A (fr)
PE (1) PE20000358A1 (fr)
PL (1) PL343635A1 (fr)
WO (1) WO1999050423A2 (fr)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7070772B2 (en) 2000-07-18 2006-07-04 The General Hospital Corporation Salicylic acid biosynthetic genes and uses thereof
JP2004520048A (ja) 2001-01-29 2004-07-08 カーギル，インコーポレーテッド 真菌耐性トランスジェニック植物
CN100412199C (zh) * 2006-04-29 2008-08-20 北京未名凯拓作物设计中心有限公司 一种提高植物中水杨酸含量的方法及其专用载体
AU2013264459A1 (en) 2012-05-25 2014-11-20 Stichting Voor De Technische Wetenschappen New plant resistance gene
CN103602693B (zh) * 2013-11-22 2017-02-01 西南大学 决明SoICS基因、用于克隆决明SoICS基因的引物及其克隆方法
BR112017025309A2 (pt) * 2015-05-27 2018-07-31 Lanzatech New Zealand Ltd micro-organismos geneticamente modificados para a fabricação de produtos derivados de corismato
CN110150137B (zh) * 2019-06-03 2020-12-29 中国农业科学院植物保护研究所 一种拟南芥nbr1/atg8f双突变体的培育方法及应用
CN111440794B (zh) * 2020-04-03 2022-09-09 河南科技学院 黄瓜基因在提高光合作用、促进植株生长和自毒作用抗性中的应用
WO2025027165A1 (fr) * 2023-08-01 2025-02-06 Basf Plant Science Company Gmbh Résistance accrue par expression d'une protéine ics

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0392225A2 (fr) * 1989-03-24 1990-10-17 Ciba-Geigy Ag Plantes transgéniques résistantes aux maladies
WO1995019443A2 (fr) * 1994-01-13 1995-07-20 Ciba-Geigy Ag Sequences d'adn anti-pathogenes et a regulation chimique, et leurs utilisations

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0392225A2 (fr) * 1989-03-24 1990-10-17 Ciba-Geigy Ag Plantes transgéniques résistantes aux maladies
WO1995019443A2 (fr) * 1994-01-13 1995-07-20 Ciba-Geigy Ag Sequences d'adn anti-pathogenes et a regulation chimique, et leurs utilisations

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
LODHI A.H. ET AL.: "Expression of bacterial isochorismate synthase in transgenic root cultures of Rubia peregrina", PLANT CELL REPORTS, vol. 16, no. 1-2, 1996, pages 54 - 57, XP002117784 *
MERCADO-BLANCO J.ET AL.: "P.fluorescence isochorismate synthase", EMBL SEQUENCE DATABASE, 8 January 1997 (1997-01-08), HEIDELBERG DE, XP002117786 *
MORENO P.R.H. ET AL.: "Elicitor-mediated induction of isochorismate synthase and accumulation of 2'3-dihodroxy benzoic acid in Catharanthus roseus cell suspension and shoot cultures", PLANT CELL REPORTS, vol. 14, no. 2-3, 1994, pages 188 - 191, XP002117787 *
SERINO L. ET AL.: "Structural genes for salicylate biosynthesis from chorismate in Pseudomonas aeruginosa", MOLECULAR AND GENERAL GENETICS., vol. 249, no. 2, 15 November 1995 (1995-11-15), BERLIN, DE, pages 217 - 228, XP002117785, ISSN: 0026-8925 *
VAN TEGELEN L.J.P. ET AL.: "Purification and cDNA cloning of isochorismate synthase from elicited cell cultures of Cantharanthus roseus", PLANT PHYSIOLOGY., vol. 119, no. 2, February 1999 (1999-02-01), ROCKVILLE US, pages 705 - 712, XP002117788, ISSN: 0032-0889 *
VERBERNE M.C. ET AL.: "Genetic modification of the biosynthesis of salicylic acid and its derivatives in plants, to increase the resistance against infection - tobacco transgenic plants construction with potential increased disease resistance", PHARM WORLD SCIENCE, vol. 17, no. 6, 1995, pages N12, XP002117783 *

Also Published As

Publication number Publication date
EP1066389A2 (fr) 2001-01-10
CA2333433A1 (fr) 1999-10-07
PE20000358A1 (es) 2000-04-27
CO5050251A1 (es) 2001-06-27
CN1295619A (zh) 2001-05-16
JP2003513608A (ja) 2003-04-15
PL343635A1 (en) 2001-08-27
MXPA00009573A (es) 2003-04-22
WO1999050423A2 (fr) 1999-10-07
AR018172A1 (es) 2001-10-31
BR9909303A (pt) 2000-11-21
AU746787B2 (en) 2002-05-02
MA24793A1 (fr) 1999-10-01
AU3602599A (en) 1999-10-18

Similar Documents

Publication Publication Date Title
WO2003012052A3 (fr) Inhibition specifique d'expression de gene par de petits arns double brin
NO345029B1 (no) Isolert nukleinsyresekvens som koder for et polypeptid med 6-desaturaseaktivitet, genkonstrukjon omfattende den isolerte nukleinsyre, vektor omfattende nukleinsyren eller genkonstruksjonen, transgen oljefrøplante omfattende samme samt fremgangsmåte for fremstilling av slike forbindelser.
WO2001059128A3 (fr) Nouveau gene d'elongase et procede de preparation d'acides gras a insaturation multiple
CY1109161T1 (el) Dna που κωδικοποιει συνθαση δεοξυ-υπουσινης φυτου, διαγονιδιακα φυτα και μεθοδος ελεγχου γηρασμου και προγραμματισμενου θανατου κυτταρουσε φυτα
WO1999020799A3 (fr) And genomique de micro-organismes, compositions et procedes associes
WO1999050423A3 (fr) Genes de la voie de l'acide salicylique et leur utilisation pour induire la resistance chez les vegetaux
EP1571219A3 (fr) Séquences régulatrices végétales pour le contrôle sélectif de l'expression génique
WO1999049050A3 (fr) Genes d'huile de limnanthes
WO2001019976A3 (fr) Sequences regulatrices utiles pour commander l'expression genique dans des plantes
DE60040497D1 (de) Gene, welche für Flavon-Synthasen kodieren
WO2003020016A3 (fr) Promoteur 7s $g(a) specifique de la semence pour expression de genes chez la plante
WO2000004154A3 (fr) Genes codant pour des proteines d'assimilation des sulfates
WO2003100074A3 (fr) Methode de genotypage d'animaux
WO2005048692A3 (fr) Tomates a activite desoxyhypusine synthase reduite par suite d'alterations non transgeniques du gene de la desoxyhypusine synthase
WO2002077158A3 (fr) Promoteur inductible stimulant specifique des racines et son utilisation
WO2001007590A3 (fr) Nouveaux gènes chimères
WO2000022145A3 (fr) Gene d'hydroperoxyde-lyase tire du mais et procedes d'utilisation
WO1999055883A3 (fr) Triacylglycerol lipases
SE9502259D0 (sv) Oligonucleotides to inhibit the expression of isoprenyl protein transferases
WO2004056848A3 (fr) Augmentation des niveaux d'huile dans des plantes de l'espece brassica
WO2004013304A3 (fr) Regulateurs du metabolisme des glucides et du metabolisme lipidique chez les plantes iv
WO2000005353A3 (fr) Chorismate synthase issue de plantes
ATE296835T1 (de) Orotidin-5'-phosphatdecarboxylase-gen, genkonstrukt enthaltend dieses gen und seine verwendung
WO2002006495A3 (fr) Activateurs specifiques du muscle, et mutants
WO2002000915A3 (fr) Fragments d'acides nucleiques cyanobacteriens codant pour des proteines utiles dans la regulation de caracteristiques vegetales par la transformation nucleaire ou plastomere

Legal Events

Date Code Title Description
WWE Wipo information: entry into national phase

Ref document number: 99804666.3

Country of ref document: CN

AK Designated states

Kind code of ref document: A2

Designated state(s): AE AL AM AT AU AZ BA BB BG BR BY CA CH CN CU CZ DE DK EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW SD SL SZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
AK Designated states

Kind code of ref document: A3

Designated state(s): AE AL AM AT AU AZ BA BB BG BR BY CA CH CN CU CZ DE DK EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): GH GM KE LS MW SD SL SZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
WWE Wipo information: entry into national phase

Ref document number: 1999917919

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 2000/05115

Country of ref document: ZA

Ref document number: 200005115

Country of ref document: ZA

ENP Entry into the national phase

Ref document number: 2333433

Country of ref document: CA

WWE Wipo information: entry into national phase

Ref document number: IN/PCT/2000/457/CHE

Country of ref document: IN

Ref document number: 36025/99

Country of ref document: AU

WWE Wipo information: entry into national phase

Ref document number: PA/a/2000/009573

Country of ref document: MX

NENP Non-entry into the national phase

Ref country code: KR

WWP Wipo information: published in national office

Ref document number: 1999917919

Country of ref document: EP

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

NENP Non-entry into the national phase

Ref country code: CA

WWG Wipo information: grant in national office

Ref document number: 36025/99

Country of ref document: AU

WWW Wipo information: withdrawn in national office

Ref document number: 1999917919

Country of ref document: EP

点击 这是indexloc提供的php浏览器服务，不要输入任何密码和下载