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WO1999050423A3 - Isochorismate synthase and its use for the induction of resistance in plants - Google Patents

Isochorismate synthase and its use for the induction of resistance in plants Download PDF

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Publication number
WO1999050423A3
WO1999050423A3 PCT/EP1999/002176 EP9902176W WO9950423A3 WO 1999050423 A3 WO1999050423 A3 WO 1999050423A3 EP 9902176 W EP9902176 W EP 9902176W WO 9950423 A3 WO9950423 A3 WO 9950423A3
Authority
WO
WIPO (PCT)
Prior art keywords
plants
isochorismate
gene
gene coding
isochorismate synthase
Prior art date
Application number
PCT/EP1999/002176
Other languages
French (fr)
Other versions
WO1999050423A2 (en
Inventor
Hubertus Josephus Ma Linthorst
Robert Verpoorte
Maria Catharina Verberne
Paulo R H Moreno
Tegelen Leonardus Johannes Van
George Joseph Wullems
Anton Felix Croes
Maarten Hendrik Stuiver
Jerome Hubertina Henri Custers
Lambertus Henricus Simons
Leo Sjoerd Melchers
John Ferdinand Bol
Original Assignee
Mogen Int
Univ Leiden
Univ Nijmegen
Linthorst Hubertus J M
Robert Verpoorte
Maria Catharina Verberne
Paulo R H Moreno
Tegelen Leonardus Johannes Pet
George Joseph Wullems
Anton Felix Croes
Maarten Hendrik Stuiver
Jerome Hubertina Henri Custers
Lambertus Henricus Simons
Leo Sjoerd Melchers
John Ferdinand Bol
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mogen Int, Univ Leiden, Univ Nijmegen, Linthorst Hubertus J M, Robert Verpoorte, Maria Catharina Verberne, Paulo R H Moreno, Tegelen Leonardus Johannes Pet, George Joseph Wullems, Anton Felix Croes, Maarten Hendrik Stuiver, Jerome Hubertina Henri Custers, Lambertus Henricus Simons, Leo Sjoerd Melchers, John Ferdinand Bol filed Critical Mogen Int
Priority to AU36025/99A priority Critical patent/AU746787B2/en
Priority to BR9909303-0A priority patent/BR9909303A/en
Priority to JP2000541311A priority patent/JP2003513608A/en
Priority to EP99917919A priority patent/EP1066389A2/en
Priority to MXPA00009573A priority patent/MXPA00009573A/en
Priority to CA002333433A priority patent/CA2333433A1/en
Publication of WO1999050423A2 publication Critical patent/WO1999050423A2/en
Publication of WO1999050423A3 publication Critical patent/WO1999050423A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237—Externally regulated expression systems
    • C12N15/8239—Externally regulated expression systems pathogen inducible
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8282—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for fungal resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8283—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for virus resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Communicable Diseases (AREA)
  • Virology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

This invention describes a method to induce pathogen resistance in plants, characterized in that plants are transformed with an expression cassette harboring a gene coding for an isochorismate synthase. More specifically, this method is characterized in that the gene coding for isochorismate synthase is selected from a group consisting of entC, orfA, pchA and ICS, this last gene preferably the ICS gene from Catharantus roseus. Another embodiment of the invention is a method according to the method described above, characterized in that plants are additionally transformed with an expression cassette harboring a gene coding for an isochorismate pyruvate lyase, preferably on the same expression cassette as the gene coding for isochorismate synthase. The gene coding for isochorismate pyruvate lyase is preferably selected from the group consisting of orfD and pchB. A further aspect of the invention is a protein having isochorismate synthase activity which is isolated from Catharantus roseus. Still a further aspect of the invention is the nucleotide sequence comprising the 5' regulatory region which is naturally found to regulate the expression of the ICS gene in Catharantus roseus.
PCT/EP1999/002176 1998-03-31 1999-03-25 Isochorismate synthase and its use for the induction of resistance in plants WO1999050423A2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
AU36025/99A AU746787B2 (en) 1998-03-31 1999-03-25 Salicylic acid pathway genes and their use for the induction of resistance in plants
BR9909303-0A BR9909303A (en) 1998-03-31 1999-03-25 Process to induce resistance to pathogen in plants, protein that has the activity of isocorismato synthase, nucleotide sequence, promoter inducible by pathogen, use of the same, vector, agrobacterium strain, plant cells, and, plants
JP2000541311A JP2003513608A (en) 1998-03-31 1999-03-25 Isochorismate synthase and its use for inducing tolerance in plants
EP99917919A EP1066389A2 (en) 1998-03-31 1999-03-25 Isochorismate synthase and its use for the induction of resistance in plants
MXPA00009573A MXPA00009573A (en) 1998-03-31 1999-03-25 Salicylic acid pathway genes and their use for the induction of resistance in plants.
CA002333433A CA2333433A1 (en) 1998-03-31 1999-03-25 Salicylic acid pathway genes and their use for the induction of resistance in plants

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US8020398P 1998-03-31 1998-03-31
US60/080,203 1998-03-31
US8062598P 1998-04-03 1998-04-03
US60/080,625 1998-04-03

Publications (2)

Publication Number Publication Date
WO1999050423A2 WO1999050423A2 (en) 1999-10-07
WO1999050423A3 true WO1999050423A3 (en) 1999-12-16

Family

ID=26763209

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1999/002176 WO1999050423A2 (en) 1998-03-31 1999-03-25 Isochorismate synthase and its use for the induction of resistance in plants

Country Status (13)

Country Link
EP (1) EP1066389A2 (en)
JP (1) JP2003513608A (en)
CN (1) CN1295619A (en)
AR (1) AR018172A1 (en)
AU (1) AU746787B2 (en)
BR (1) BR9909303A (en)
CA (1) CA2333433A1 (en)
CO (1) CO5050251A1 (en)
MA (1) MA24793A1 (en)
MX (1) MXPA00009573A (en)
PE (1) PE20000358A1 (en)
PL (1) PL343635A1 (en)
WO (1) WO1999050423A2 (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7070772B2 (en) 2000-07-18 2006-07-04 The General Hospital Corporation Salicylic acid biosynthetic genes and uses thereof
EP1363487A4 (en) 2001-01-29 2005-08-17 Cargill Inc Fungal resistant transgenic plants
CN100412199C (en) * 2006-04-29 2008-08-20 北京未名凯拓作物设计中心有限公司 Method for increasing salicylic acid content in plant, and its special carrier
WO2013176548A1 (en) 2012-05-25 2013-11-28 Wageningen Universiteit New plant resistance gene
CN103602693B (en) * 2013-11-22 2017-02-01 西南大学 Semen cassiae SoICS gene, primer for cloning semen cassiae SoICS gene and cloning method thereof
EP3303594A4 (en) * 2015-05-27 2018-12-05 Lanzatech New Zealand Limited Genetically engineered microorganisms for the production of chorismate-derived products
CN110150137B (en) * 2019-06-03 2020-12-29 中国农业科学院植物保护研究所 Breeding method and application of an Arabidopsis nbr1/atg8f double mutant
CN111440794B (en) * 2020-04-03 2022-09-09 河南科技学院 Application of cucumber gene in improving photosynthesis, promoting plant growth and autotoxicity resistance
WO2025027165A1 (en) * 2023-08-01 2025-02-06 Basf Plant Science Company Gmbh Increased resistance by expression of an ics protein

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0392225A2 (en) * 1989-03-24 1990-10-17 Ciba-Geigy Ag Disease-resistant transgenic plants
WO1995019443A2 (en) * 1994-01-13 1995-07-20 Ciba-Geigy Ag Chemically regulatable and anti-pathogenic dna sequences and uses thereof

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0392225A2 (en) * 1989-03-24 1990-10-17 Ciba-Geigy Ag Disease-resistant transgenic plants
WO1995019443A2 (en) * 1994-01-13 1995-07-20 Ciba-Geigy Ag Chemically regulatable and anti-pathogenic dna sequences and uses thereof

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
LODHI A.H. ET AL.: "Expression of bacterial isochorismate synthase in transgenic root cultures of Rubia peregrina", PLANT CELL REPORTS, vol. 16, no. 1-2, 1996, pages 54 - 57, XP002117784 *
MERCADO-BLANCO J.ET AL.: "P.fluorescence isochorismate synthase", EMBL SEQUENCE DATABASE, 8 January 1997 (1997-01-08), HEIDELBERG DE, XP002117786 *
MORENO P.R.H. ET AL.: "Elicitor-mediated induction of isochorismate synthase and accumulation of 2'3-dihodroxy benzoic acid in Catharanthus roseus cell suspension and shoot cultures", PLANT CELL REPORTS, vol. 14, no. 2-3, 1994, pages 188 - 191, XP002117787 *
SERINO L. ET AL.: "Structural genes for salicylate biosynthesis from chorismate in Pseudomonas aeruginosa", MOLECULAR AND GENERAL GENETICS., vol. 249, no. 2, 15 November 1995 (1995-11-15), BERLIN, DE, pages 217 - 228, XP002117785, ISSN: 0026-8925 *
VAN TEGELEN L.J.P. ET AL.: "Purification and cDNA cloning of isochorismate synthase from elicited cell cultures of Cantharanthus roseus", PLANT PHYSIOLOGY., vol. 119, no. 2, February 1999 (1999-02-01), ROCKVILLE US, pages 705 - 712, XP002117788, ISSN: 0032-0889 *
VERBERNE M.C. ET AL.: "Genetic modification of the biosynthesis of salicylic acid and its derivatives in plants, to increase the resistance against infection - tobacco transgenic plants construction with potential increased disease resistance", PHARM WORLD SCIENCE, vol. 17, no. 6, 1995, pages N12, XP002117783 *

Also Published As

Publication number Publication date
MA24793A1 (en) 1999-10-01
AU746787B2 (en) 2002-05-02
AU3602599A (en) 1999-10-18
PE20000358A1 (en) 2000-04-27
MXPA00009573A (en) 2003-04-22
WO1999050423A2 (en) 1999-10-07
CA2333433A1 (en) 1999-10-07
CO5050251A1 (en) 2001-06-27
EP1066389A2 (en) 2001-01-10
PL343635A1 (en) 2001-08-27
CN1295619A (en) 2001-05-16
AR018172A1 (en) 2001-10-31
JP2003513608A (en) 2003-04-15
BR9909303A (en) 2000-11-21

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