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WO1998050570A3 - Methods and compositions for use of (iso)eugenol methyltransferase - Google Patents

Methods and compositions for use of (iso)eugenol methyltransferase Download PDF

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Publication number
WO1998050570A3
WO1998050570A3 PCT/US1998/009522 US9809522W WO9850570A3 WO 1998050570 A3 WO1998050570 A3 WO 1998050570A3 US 9809522 W US9809522 W US 9809522W WO 9850570 A3 WO9850570 A3 WO 9850570A3
Authority
WO
WIPO (PCT)
Prior art keywords
compositions
methyltransferase
eugenol
iso
methods
Prior art date
Application number
PCT/US1998/009522
Other languages
French (fr)
Other versions
WO1998050570A2 (en
Inventor
Eran Pichersky
Jihong Wang
Original Assignee
Univ Michigan
Eran Pichersky
Jihong Wang
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Michigan, Eran Pichersky, Jihong Wang filed Critical Univ Michigan
Priority to AU73772/98A priority Critical patent/AU7377298A/en
Publication of WO1998050570A2 publication Critical patent/WO1998050570A2/en
Publication of WO1998050570A3 publication Critical patent/WO1998050570A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1003—Transferases (2.) transferring one-carbon groups (2.1)
    • C12N9/1007—Methyltransferases (general) (2.1.1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8255—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving lignin biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Nutrition Science (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention concerns DNA compositions and their use in manipulating the biosynthesis of compound in plants. More specifically, the present invention has identified a novel gene and protein involved in the phenylpropanoid pathways. S-adenosyl-L-Met:(iso)eugenol O-methyltransferase (IEMT) catalyzes a methyl transfer to the C4 hydroxyl position of compounds of the phenylpropanoid biosynthetic pathway. Methods and compositions for the use thereof are disclosed herein.
PCT/US1998/009522 1997-05-08 1998-05-08 Methods and compositions for use of (iso)eugenol methyltransferase WO1998050570A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU73772/98A AU7377298A (en) 1997-05-08 1998-05-08 Methods and compositions for use of (iso)eugenol methyltransferase

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US4685797P 1997-05-08 1997-05-08
US60/046,857 1997-05-08

Publications (2)

Publication Number Publication Date
WO1998050570A2 WO1998050570A2 (en) 1998-11-12
WO1998050570A3 true WO1998050570A3 (en) 1999-02-04

Family

ID=21945772

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1998/009522 WO1998050570A2 (en) 1997-05-08 1998-05-08 Methods and compositions for use of (iso)eugenol methyltransferase

Country Status (2)

Country Link
AU (1) AU7377298A (en)
WO (1) WO1998050570A2 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000050613A2 (en) * 1999-02-22 2000-08-31 Yissum Research And Development Company Of The Hebrew University Of Jerusalem Transgenic plants and method for transforming carnations
US7129393B1 (en) 1999-02-22 2006-10-31 Yissum Research Development Company Of The Hebrew University Transgenic plants and method for transforming carnations
NZ532712A (en) 2001-11-07 2006-12-22 Genesis Res & Dev Corp Ltd Polynucleotides isolated from forage grass tissues, specifically Lolium perenne (perennial ryegrass) and Festuca arundinacea (tall fescue)
NL2004624C2 (en) * 2010-04-28 2011-11-01 Stichting Dienst Landbouwkundi A new glycosyltransferase protein and its role in the metabolism of phenylpropanoid volatiles in tomato.
US8889392B2 (en) * 2010-10-28 2014-11-18 Brookhaven Science Associates, Llc Monolignol 4-O-methyltransferases and uses thereof
US9556420B2 (en) * 2013-06-18 2017-01-31 Brookhaven Science Associates, Llc Specialized (iso)eugenol-4-O-methyltransferases (s-IEMTs) and methods of making and using the same

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993005160A1 (en) * 1991-09-10 1993-03-18 Zeneca Limited Modification of lignin synthesis in plants
WO1997015584A2 (en) * 1995-10-12 1997-05-01 The Regents Of The University Of Michigan Use of linalool synthase in genetic engineering of scent production

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993005160A1 (en) * 1991-09-10 1993-03-18 Zeneca Limited Modification of lignin synthesis in plants
WO1997015584A2 (en) * 1995-10-12 1997-05-01 The Regents Of The University Of Michigan Use of linalool synthase in genetic engineering of scent production

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
WANG,J. AND PICHERSKY, E.: "characterization of S-adenosyl-L-methionine:(iso)eugenol O-methyltransferase involved in floral scent production in Clarkia breweri", ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, vol. 349, no. 1, 1998, pages 153 - 160, XP002083952 *
WANG,J., ET AL.: "expression of genes involved in floral scent production in Clarkia", PLANT PHYSIOLOGY, SUPPLEMENT, vol. 114, no. 3, July 1997 (1997-07-01), pages 232, XP002083953 *
WANG,J., ET AL.: "floral scent production in Clarkia breweri (Onagraceae)", PLANT PHYSIOLOGY, vol. 114, May 1997 (1997-05-01), pages 213 - 221, XP002083951 *
WEITING, N.L., ET AL: "REDUCED LIGNIN IN TRANSGENIC PLANTS CONTAINING A CAFFEIC ACID O-METHYLTRANSFERASE ANTISENSE GENE", TRANSGENIC RESEARCH, vol. 3, no. 2, March 1994 (1994-03-01), pages 120 - 126, XP002054140 *

Also Published As

Publication number Publication date
WO1998050570A2 (en) 1998-11-12
AU7377298A (en) 1998-11-27

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