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WO1997039326A3 - Dosage in vitro a polarisation de fluorescence - Google Patents

Dosage in vitro a polarisation de fluorescence Download PDF

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Publication number
WO1997039326A3
WO1997039326A3 PCT/US1997/006746 US9706746W WO9739326A3 WO 1997039326 A3 WO1997039326 A3 WO 1997039326A3 US 9706746 W US9706746 W US 9706746W WO 9739326 A3 WO9739326 A3 WO 9739326A3
Authority
WO
WIPO (PCT)
Prior art keywords
proteins
test substance
emission
mixture
pair
Prior art date
Application number
PCT/US1997/006746
Other languages
English (en)
Other versions
WO1997039326A2 (fr
Inventor
Berkley A Lynch
Ian A Macneil
Mark J Zoller
Original Assignee
Ariad Pharma Inc
Berkley A Lynch
Ian A Macneil
Mark J Zoller
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ariad Pharma Inc, Berkley A Lynch, Ian A Macneil, Mark J Zoller filed Critical Ariad Pharma Inc
Priority to AU28076/97A priority Critical patent/AU727108B2/en
Priority to JP09530451A priority patent/JP2000512737A/ja
Priority to EP97922393A priority patent/EP0898709A2/fr
Publication of WO1997039326A2 publication Critical patent/WO1997039326A2/fr
Publication of WO1997039326A3 publication Critical patent/WO1997039326A3/fr

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Peptides Or Proteins (AREA)

Abstract

L'invention concerne un procédé de dosage in vitro permettant d'identifier une substance test qui inhibe l'association mutuelle d'une paire de protéines. Ce procédé consiste à produire une paire de protéines aptes à s'associer, l'une des protéins étant porteuse d'une substande fluorescente liée de manière covalente; à préparer un mélange contenant les deux protéins et au moins une substance test; à irradier le mélange avec de la lumière polarisée d'une longueur d'onde appropriée permettant l'excitation de la substance fluorescente telle que l'indique l'émission de lumière polarisée; à mesurer le degré de polarisation de l'émission et à déterminer l'effet dû à la présence ou à la concentration de la substance test en réduisant la polarisation de l'émission observée d'un mélange de deux protéines seules. L'activité inhibitrice de la substance test est en corrélation avec les valeurs réduites de dépolarisation.
PCT/US1997/006746 1996-04-18 1997-04-18 Dosage in vitro a polarisation de fluorescence WO1997039326A2 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
AU28076/97A AU727108B2 (en) 1996-04-18 1997-04-18 In vitro fluorescence polarization assay
JP09530451A JP2000512737A (ja) 1996-04-18 1997-04-18 試験管内蛍光偏光検定法
EP97922393A EP0898709A2 (fr) 1996-04-18 1997-04-18 DOSAGE $i(IN VITRO) A POLARISATION DE FLUORESCENCE

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US1559096P 1996-04-18 1996-04-18
US60/015,590 1996-04-18
US2987096P 1996-11-06 1996-11-06
US60/029,870 1996-11-06

Publications (2)

Publication Number Publication Date
WO1997039326A2 WO1997039326A2 (fr) 1997-10-23
WO1997039326A3 true WO1997039326A3 (fr) 1997-12-24

Family

ID=26687596

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1997/006746 WO1997039326A2 (fr) 1996-04-18 1997-04-18 Dosage in vitro a polarisation de fluorescence

Country Status (5)

Country Link
EP (1) EP0898709A2 (fr)
JP (1) JP2000512737A (fr)
AU (1) AU727108B2 (fr)
CA (1) CA2250067A1 (fr)
WO (1) WO1997039326A2 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7619059B2 (en) 2003-07-29 2009-11-17 Life Technologies Corporation Bimolecular optical probes

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0938586A4 (fr) * 1996-10-28 2002-01-30 Panvera Corp Mesure de l'activite de la kinase par polarisation de fluorescence
US7105307B2 (en) 1997-08-30 2006-09-12 Cyclacel, Ltd. Compositions and methods for screening for modulators of enzymatic activity
KR20000068915A (ko) * 1997-09-05 2000-11-25 마쓰시다 일렉트릭 인더스트리얼 컴패니 리미티드 형광 편광법
GB9923208D0 (en) * 1999-10-01 1999-12-08 Cambridge Drug Discovery Ltd Assay
UY27304A1 (es) 2001-05-24 2002-12-31 Avanir Pharmaceuticals Inhibidores del factor inhibidor de la migración de los macrófagos y métodos para su identificación
US7763439B2 (en) 2001-07-02 2010-07-27 Arctic Diagnostics Oy Two-photon absorbing dipyrrometheneboron difluoride dyes and their applications
JP4382474B2 (ja) 2001-07-02 2009-12-16 アークティク ダイアグノスティックス オサケユイチア 蛍光ラベル化合物の親水性を増加させる方法
TW200418829A (en) 2003-02-14 2004-10-01 Avanir Pharmaceutics Inhibitors of macrophage migration inhibitory factor and methods for identifying the same
US7727752B2 (en) 2003-07-29 2010-06-01 Life Technologies Corporation Kinase and phosphatase assays
CA2531506A1 (fr) 2003-08-22 2005-03-10 Avanir Pharmaceuticals Derives de naphtyridine substitues servant d'inhibiteurs de facteur d'inhibition de la migration des macrophages et leur utilisation pour traiter des maladies humaines
JP4607950B2 (ja) * 2004-03-18 2011-01-05 トランス テック ファーマ，インコーポレイテッド 蛍光偏光アッセイ
US7608407B2 (en) * 2004-11-16 2009-10-27 Boehringer Ingelheim Pharmaceuticals, Inc. Fluorescence polarization assay for determining histidine decarboxylase activity
CA2600175A1 (fr) 2005-03-24 2006-03-20 Avanir Pharmaceuticals Derives de thienopyridinone utilises en tant qu'inhibiteurs du facteur d'inhibition de la migration des macrophages
US9164086B2 (en) 2011-10-18 2015-10-20 Max-Planck-Gesellschaft Zur Foerderung Der Wissenschaften E.V. Fluorescent dyes with phosphorylated hydroxymethyl groups and their use in light microscopy and imaging techniques
EP2944958A1 (fr) 2014-04-04 2015-11-18 Techno-Path (Distribution) Procédé de prédiction d'instabilité phénotypique dans une cellule
EP3795999A1 (fr) * 2014-07-15 2021-03-24 Valitacell Limited Procédé permettant de déterminer l'abondance d'une molécule cible dans un échantillon
EP3075844A1 (fr) 2015-04-01 2016-10-05 Valitacell Limited Procédé de détermination d'une caractéristique fonctionnelle ou de composition d'un milieu de culture cellulaire

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4257774A (en) * 1979-07-16 1981-03-24 Meloy Laboratories, Inc. Intercalation inhibition assay for compounds that interact with DNA or RNA
EP0449269A1 (fr) * 1990-03-27 1991-10-02 Roche Diagnostics GmbH Méthode pour la détection des protéines contenant tyrosine phosphorylée
WO1993003377A1 (fr) * 1991-07-31 1993-02-18 Ziltener Hermann J Procede de detection de restes de phosphotyrosine
US5352660A (en) * 1991-10-31 1994-10-04 Mount Sinai Hospital Corporation Method for assaying for a substance that affects a SH2-phosphorylated ligand regulatory system
WO1997010253A1 (fr) * 1995-09-15 1997-03-20 Merck & Co., Inc. Dosage a rendement eleve utilisant des proteines hybrides

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4257774A (en) * 1979-07-16 1981-03-24 Meloy Laboratories, Inc. Intercalation inhibition assay for compounds that interact with DNA or RNA
EP0449269A1 (fr) * 1990-03-27 1991-10-02 Roche Diagnostics GmbH Méthode pour la détection des protéines contenant tyrosine phosphorylée
WO1993003377A1 (fr) * 1991-07-31 1993-02-18 Ziltener Hermann J Procede de detection de restes de phosphotyrosine
US5352660A (en) * 1991-10-31 1994-10-04 Mount Sinai Hospital Corporation Method for assaying for a substance that affects a SH2-phosphorylated ligand regulatory system
WO1997010253A1 (fr) * 1995-09-15 1997-03-20 Merck & Co., Inc. Dosage a rendement eleve utilisant des proteines hybrides

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
B. A. LYNCH ET AL: "A fluorescence polarisation based Src-SH2 binding assay.", ANALYTICAL BIOCHEMISTRY., vol. 247, no. 1, 1997, NEW YORK US, pages 77 - 82, XP002045666 *
P. WU ET AL: "A high-throughput STAT binding assay using fluorescence polatization.", ANALYTICAL BIOCHEMISTRY., vol. 249, no. 1, 1997, NEW YORK US, pages 29 - 36, XP002045667 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7619059B2 (en) 2003-07-29 2009-11-17 Life Technologies Corporation Bimolecular optical probes

Also Published As

Publication number Publication date
CA2250067A1 (fr) 1997-10-23
JP2000512737A (ja) 2000-09-26
AU2807697A (en) 1997-11-07
AU727108B2 (en) 2000-11-30
WO1997039326A2 (fr) 1997-10-23
EP0898709A2 (fr) 1999-03-03

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