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WO1997006268A3 - Constructions d'adn - Google Patents

Constructions d'adn Download PDF

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Publication number
WO1997006268A3
WO1997006268A3 PCT/GB1996/001846 GB9601846W WO9706268A3 WO 1997006268 A3 WO1997006268 A3 WO 1997006268A3 GB 9601846 W GB9601846 W GB 9601846W WO 9706268 A3 WO9706268 A3 WO 9706268A3
Authority
WO
WIPO (PCT)
Prior art keywords
protein
gene
insects
encodes
damaging
Prior art date
Application number
PCT/GB1996/001846
Other languages
English (en)
Other versions
WO1997006268A2 (fr
Inventor
Ian Jepson
Jacqueline Ann Mary Paine
Original Assignee
Zeneca Ltd
Ian Jepson
Jacqueline Ann Mary Paine
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Zeneca Ltd, Ian Jepson, Jacqueline Ann Mary Paine filed Critical Zeneca Ltd
Priority to PL96324880A priority Critical patent/PL324880A1/xx
Priority to CA002227445A priority patent/CA2227445A1/fr
Priority to APAP/P/1998/001194A priority patent/AP863A/en
Priority to IL12317296A priority patent/IL123172A0/xx
Priority to AU66252/96A priority patent/AU704172B2/en
Priority to BR9609873A priority patent/BR9609873A/pt
Priority to SK169-98A priority patent/SK16998A3/sk
Priority to EP96925889A priority patent/EP0846179A2/fr
Priority to MX9801008A priority patent/MX9801008A/es
Priority to JP9508208A priority patent/JPH11510694A/ja
Priority to NZ313724A priority patent/NZ313724A/en
Publication of WO1997006268A2 publication Critical patent/WO1997006268A2/fr
Publication of WO1997006268A3 publication Critical patent/WO1997006268A3/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237—Externally regulated expression systems
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237—Externally regulated expression systems
    • C12N15/8238—Externally regulated expression systems chemically inducible, e.g. tetracycline
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Insects & Arthropods (AREA)
  • Pest Control & Pesticides (AREA)
  • General Chemical & Material Sciences (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Peptides Or Proteins (AREA)

Abstract

On décrit une cassette d'expression de gènes de plantes pouvant être induite chimiquement, qui comprend un premier promoteur lié de manière fonctionnelle à une séquence de régulation, laquelle est dérivée du gène alcR et code une protéine régulatrice, ainsi qu'un promoteur pouvant être induit et qui est lié de manière fonctionnelle à un gène cible codant une protéine, laquelle est préjudiciable aux insectes, ou dont l'expression induit un processus métabolique produisant un métabolite, lequel est préjudiciable aux insectes. Le promoteur pouvant être induit est activé par la protéine régulatrice en présence d'un inducteur exogène efficace, une application de cet inducteur provoquant l'expression du gène cible.
PCT/GB1996/001846 1995-08-08 1996-07-29 Constructions d'adn WO1997006268A2 (fr)

Priority Applications (11)

Application Number Priority Date Filing Date Title
PL96324880A PL324880A1 (en) 1995-08-08 1996-07-29 Dna constructs
CA002227445A CA2227445A1 (fr) 1995-08-08 1996-07-29 Constructions d'adn
APAP/P/1998/001194A AP863A (en) 1995-08-08 1996-07-29 Dna constructs.
IL12317296A IL123172A0 (en) 1995-08-08 1996-07-29 Dna constructs
AU66252/96A AU704172B2 (en) 1995-08-08 1996-07-29 DNA constructs
BR9609873A BR9609873A (pt) 1995-08-08 1996-07-29 Cassete de expressão de gene de planta quimicamente indutível célula de planta tecido de planta planta semente e processo par controlar insetos
SK169-98A SK16998A3 (en) 1995-08-08 1996-07-29 Chemically-inducible plant gene expression cassette
EP96925889A EP0846179A2 (fr) 1995-08-08 1996-07-29 Constructions d'adn
MX9801008A MX9801008A (es) 1995-08-08 1996-07-29 Constructos de adn.
JP9508208A JPH11510694A (ja) 1995-08-08 1996-07-29 Ｄｎａ構築体
NZ313724A NZ313724A (en) 1995-08-08 1996-07-29 Dna constructs and promoter sequences and their use in the expression of genes which confer insecticidal activity on plants

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB9516241.8 1995-08-08
GBGB9516241.8A GB9516241D0 (en) 1995-08-08 1995-08-08 Dna constructs

Publications (2)

Publication Number Publication Date
WO1997006268A2 WO1997006268A2 (fr) 1997-02-20
WO1997006268A3 true WO1997006268A3 (fr) 1997-03-13

Family

ID=10778942

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB1996/001846 WO1997006268A2 (fr) 1995-08-08 1996-07-29 Constructions d'adn

Country Status (19)

Country Link
EP (1) EP0846179A2 (fr)
JP (1) JPH11510694A (fr)
KR (1) KR19990036251A (fr)
CN (1) CN1199425A (fr)
AP (1) AP863A (fr)
AR (1) AR002914A1 (fr)
AU (1) AU704172B2 (fr)
BR (1) BR9609873A (fr)
CA (1) CA2227445A1 (fr)
CZ (1) CZ36998A3 (fr)
GB (1) GB9516241D0 (fr)
HU (1) HUP9900057A3 (fr)
IL (1) IL123172A0 (fr)
MX (1) MX9801008A (fr)
NZ (1) NZ313724A (fr)
PL (1) PL324880A1 (fr)
SK (1) SK16998A3 (fr)
TR (1) TR199800177T1 (fr)
WO (1) WO1997006268A2 (fr)

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GB9606062D0 (en) 1996-03-22 1996-05-22 Zeneca Ltd Promoters
GB9817704D0 (en) * 1998-08-13 1998-10-07 Zeneca Ltd Gene switch
GB9902234D0 (en) * 1999-02-01 1999-03-24 Zeneca Ltd Expression system
GB9902236D0 (en) * 1999-02-01 1999-03-24 Zeneca Ltd Formulation
GB9918154D0 (en) * 1999-08-02 1999-10-06 Zeneca Ltd Expression system
EP1925672A1 (fr) 2001-06-22 2008-05-28 Syngeta Participations AG Polynucléotides et polypeptides sensibles au stress abiotique
DE10212158A1 (de) 2002-03-19 2003-10-02 Metanomics Gmbh & Co Kgaa Population transgener Pflanzen, davon abgeleitetes biologisches Material, entsprechende Plasmidkollektion und Population transformierter Wirtsorganismen, sowie deren Verwendung und Verfahren zu deren Erzeugung
CN100552030C (zh) 2002-12-20 2009-10-21 梅坦诺米克斯有限及两合公司 制备氨基酸的方法
EP2322633A3 (fr) 2003-02-17 2011-08-17 Metanomics GmbH Préparation d'organismes dotés d'une croissance plus rapide et/ou d'un meilleur rendement
CN1852985A (zh) 2003-08-01 2006-10-25 巴斯福植物科学有限公司 产生精细化学品的方法
WO2006069610A2 (fr) 2004-07-02 2006-07-06 Metanomics Gmbh Procede de production de produits chimiques fins
US20100050296A1 (en) 2004-07-31 2010-02-25 Metanomics Gmbh Preparation of organisms with faster growth and/or higher yield
MX2007007040A (es) 2004-12-17 2008-10-24 Metanomics Gmbh Proceso para el control de produccion de productos quimicos finos.
EP2573188A2 (fr) 2005-03-02 2013-03-27 Metanomics GmbH Procédé de production de produits chimiques fins
AU2006219823A1 (en) 2005-03-02 2006-09-08 Metanomics Gmbh Process for the production of fine chemicals
EP2166102B1 (fr) 2005-03-08 2012-12-19 BASF Plant Science GmbH Expression à amélioration de séquences d'intron
BRPI0612710A2 (pt) 2005-07-06 2012-10-02 Cropdesign Nv método para aumentar o rendimento de plantas com relação às plantas de controle, e para produzir uma planta transgênica tendo rendimento aumentado, planta, construção, partes coletáveis de uma planta, produtos, e, uso de um ácido nucleico / gene de tipo ste20 ou variante do mesmo, ou uso de um polipeptìdeo de tipo ste20 ou seu homólogo
CA2621489A1 (fr) 2005-09-15 2007-03-22 Cropdesign N.V. Plantes a rendement accru et procede pour produire ces plantes
EP2199398A1 (fr) 2006-03-31 2010-06-23 BASF Plant Science GmbH Plantes avec des rendements améliorés et méthodes pour les produire
CA2644273A1 (fr) 2006-04-05 2008-03-27 Metanomics Gmbh Procede de production d'un produit chimique fin
MX2008015090A (es) 2006-05-31 2008-12-15 Metanomics Gmbh Manipulacion del metabolismo del nitrogeno.
CA2649341A1 (fr) 2006-06-08 2007-12-13 Basf Plant Science Gmbh Vegetaux aux caracteristiques de croissance ameliorees et procede d'obtention
EP2540832A1 (fr) 2006-08-02 2013-01-02 CropDesign N.V. Installations dotées de caractéristiques améliorées, transformées avec un petit kinase inductible, et procédé de fabrication de celles-ci
ITUD20060280A1 (it) 2006-12-29 2008-06-30 Univ Degli Studi Udine Sequenza artificiale di dna evente funzione di leader in 5' (5'-utr) ottimizzata per la sovraespressione di proteine eterologhe in pianta
EP2492345A3 (fr) 2007-05-04 2012-12-12 BASF Plant Science GmbH Amélioration de semences par des combinaisons de pyruvate kinase
EP2074220A2 (fr) 2007-05-22 2009-07-01 BASF Plant Science GmbH Cellules vegetales et plantes presentant une tolérance et/ou une resistance accrues au stress environnemental et une production-ko de biomasse accrue
CA2704271C (fr) 2007-12-03 2016-07-19 Syngenta Participations Ag Construction de phytases enzymatiquement sensibles
AU2008340002A1 (en) 2007-12-21 2009-07-02 Basf Plant Science Gmbh Plants with increased yield (KO NUE)
WO2011074959A1 (fr) 2009-12-15 2011-06-23 Edwin Henricus Antonius Holman Végétaux transgéniques résistants à l'ozone
AR083029A1 (es) 2010-12-09 2013-01-23 Syngenta Participations Ag Metodos y composiciones que utilizan arn interferente pequeño (arnip) para el control de nematodos en plantas
CN103314115A (zh) 2011-01-18 2013-09-18 先正达参股股份有限公司 用于改性乙醇诱导型启动子系统的方法及组合物
US8951752B2 (en) 2011-10-20 2015-02-10 University Of Iowa Research Foundation N-demethyase genes and uses thereof
ES2670505T3 (es) 2012-02-16 2018-05-30 Syngenta Participations Ag Proteínas pesticidas modificadas
EP2861740A1 (fr) 2012-06-15 2015-04-22 University of Iowa Research Foundation Promoteurs sensibles à la caféine et gènes régulateurs
BR112014031789A2 (pt) 2012-06-22 2017-08-01 Syngenta Participations Ag controle biológico de pragas de coleópteros
EP2895610B1 (fr) 2012-09-13 2019-11-06 Indiana University Research and Technology Corporation Compositions et systèmes pour conférer une résistance à une maladie à des plantes et leurs procédés d'utilisation
AU2015248815B2 (en) 2014-04-17 2021-07-08 Boehringer Ingelheim Rcv Gmbh & Co Kg Recombinant host cell engineered to overexpress helper proteins
BR112016023769A2 (pt) 2014-04-17 2017-10-17 Boehringer Ingelheim Rcv Gmbh célula hospedeira recombinante para a expressão de proteínas de interesse
EP2952584A1 (fr) 2014-06-04 2015-12-09 Boehringer Ingelheim RCV GmbH & Co KG Production de protéines améliorée
WO2016100333A1 (fr) 2014-12-15 2016-06-23 Syngenta Participations Ag Supports de micro-arn pesticides et leur utilisation
MX2017007904A (es) 2014-12-23 2017-09-05 Syngenta Participations Ag Control biologico de plagas de coleopteros.
UA125114C2 (uk) 2015-04-10 2022-01-12 Сінгента Партісіпейшнс Аг Кормова композиція для тварин і способи її застосування
WO2018076335A1 (fr) 2016-10-31 2018-05-03 Institute Of Genetics And Developmental Biology, Chinese Academy Of Sciences Compositions et procédés pour améliorer la tolérance au stress abiotique
JP7189145B2 (ja) 2017-03-29 2022-12-13 ベーリンガー インゲルハイム エルツェーファウ ゲゼルシャフト ミット ベシュレンクテル ハフツング ウント コンパニー コマンディトゲゼルシャフト 改変された膜脂質組成を有するリコンビナントホスト細胞
EP3625340A4 (fr) 2017-05-18 2021-02-24 Cargill, Incorporated Système d'édition de génome
EP3691453A4 (fr) 2017-10-02 2021-07-07 Syngenta Participations AG Protéines pesticides génétiquement modifiées et procédés de lutte contre les parasites des plantes
US11905518B2 (en) 2018-02-12 2024-02-20 Curators Of The University Of Missouri Small auxin upregulated (SAUR) gene for the improvement of root system architecture, waterlogging tolerance, drought resistance and yield in plants and methods of uses
US12258374B2 (en) 2019-02-20 2025-03-25 Syngenta Crop Protection Ag Engineered pesticidal proteins and methods of controlling plant pests
KR20230144629A (ko) 2021-02-12 2023-10-16 베링거 인겔하임 에르체파우 게엠베하 운트 코 카게 단백질 분비 증가를 위한 신호 펩타이드

Citations (4)

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Publication number Priority date Publication date Assignee Title
EP0159884A2 (fr) * 1984-04-13 1985-10-30 Lubrizol Genetics Inc. Promoteur et gène de choc thermique
WO1993007278A1 (fr) * 1991-10-04 1993-04-15 Ciba-Geigy Ag Sequence d'adn synthetique ayant une action insecticide accrue dans le mais
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WO1995014098A1 (fr) * 1993-11-19 1995-05-26 Biotechnology Research And Development Corporation Regions regulatrices chimeres et cassettes de genes destines a l'expression de genes dans des plantes

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0159884A2 (fr) * 1984-04-13 1985-10-30 Lubrizol Genetics Inc. Promoteur et gène de choc thermique
WO1993007278A1 (fr) * 1991-10-04 1993-04-15 Ciba-Geigy Ag Sequence d'adn synthetique ayant une action insecticide accrue dans le mais
WO1993021334A1 (fr) * 1992-04-13 1993-10-28 Zeneca Limited Produits de recombinaison d'adn et plantes les incorporant
WO1995014098A1 (fr) * 1993-11-19 1995-05-26 Biotechnology Research And Development Corporation Regions regulatrices chimeres et cassettes de genes destines a l'expression de genes dans des plantes

Non-Patent Citations (1)

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Title
LOCKINGTON, R.A. ET AL.: "Cloning and characterization of the ethanol utilization regulon in Aspergillus nidulans", GENE, vol. 33, 1985, pages 137 - 149, XP002022378 *

Also Published As

Publication number Publication date
KR19990036251A (ko) 1999-05-25
CZ36998A3 (cs) 1998-06-17
AP9801194A0 (en) 1998-03-31
EP0846179A2 (fr) 1998-06-10
AU6625296A (en) 1997-03-05
TR199800177T1 (xx) 1998-05-21
JPH11510694A (ja) 1999-09-21
AP863A (en) 2000-08-04
WO1997006268A2 (fr) 1997-02-20
AU704172B2 (en) 1999-04-15
CN1199425A (zh) 1998-11-18
GB9516241D0 (en) 1995-10-11
MX9801008A (es) 1998-04-30
AR002914A1 (es) 1998-04-29
IL123172A0 (en) 1998-09-24
SK16998A3 (en) 1998-09-09
HUP9900057A3 (en) 2001-06-28
CA2227445A1 (fr) 1997-02-20
HUP9900057A2 (hu) 1999-04-28
PL324880A1 (en) 1998-06-22
BR9609873A (pt) 1999-03-23
NZ313724A (en) 1999-04-29

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