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WO1997044667A3 - Methodes d'utilisation de complexes peptide/complexe majeur d'histocompatibilite pour obtenir ou purifier des cellules T antigene-specifiques et pour stimuler des cellules T - Google Patents

Methodes d'utilisation de complexes peptide/complexe majeur d'histocompatibilite pour obtenir ou purifier des cellules T antigene-specifiques et pour stimuler des cellules T Download PDF

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Publication number
WO1997044667A3
WO1997044667A3 PCT/FR1997/000892 FR9700892W WO9744667A3 WO 1997044667 A3 WO1997044667 A3 WO 1997044667A3 FR 9700892 W FR9700892 W FR 9700892W WO 9744667 A3 WO9744667 A3 WO 9744667A3
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WIPO (PCT)
Prior art keywords
peptide
specific
lymphocytes
mhc
cells
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Application number
PCT/FR1997/000892
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English (en)
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WO1997044667A2 (fr
Inventor
Pierre Langlade-Demoyen
Yu-Chun Lone
Philippe Kourilsky
Jean-Pierre Abastado
Original Assignee
Pasteur Institut
Inst Nat Sante Rech Med
Pierre Langlade-Demoyen
Yu-Chun Lone
Philippe Kourilsky
Jean-Pierre Abastado
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Publication date
Application filed by Pasteur Institut, Inst Nat Sante Rech Med, Pierre Langlade-Demoyen, Yu-Chun Lone, Philippe Kourilsky, Jean-Pierre Abastado filed Critical Pasteur Institut
Priority to AU30365/97A priority Critical patent/AU3036597A/en
Publication of WO1997044667A2 publication Critical patent/WO1997044667A2/fr
Publication of WO1997044667A3 publication Critical patent/WO1997044667A3/fr

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70503—Immunoglobulin superfamily
    • C07K14/70539—MHC-molecules, e.g. HLA-molecules
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • G01N33/56977—HLA or MHC typing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/16011—Orthomyxoviridae
    • C12N2760/16111—Influenzavirus A, i.e. influenza A virus
    • C12N2760/16122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Hematology (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Organic Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Virology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Oncology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Hospice & Palliative Care (AREA)
  • Toxicology (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

La présente invention concerne des méthodes d'utilisation de complexes CMH/peptide. On décrit la purification réussie de lymphocytes T peptide-spécifiques par adsorption sur un support solide couplé à une densité élevée de molécules recombinantes du CMH, chargées d'un seul peptide. Cette méthode a permis d'isoler, purifier et quantifier des lymphocytes T spécifiques de tumeur, à partir d'échantillons d'exsudat péritonéal et de lymphocytes infiltrant des tumeurs. Ces populations de lymphocytes T purifiés peuvent être encore multipliées in vitro. Les cellules résultantes présentent une activité cytotoxique supérieure et une action anti-tumorale accrue par réinjection au porteur de la tumeur. Cette nouvelle approche est importante pour analyser le répertoire de lymphocytes T au niveau de la spécificité peptidique et pour améliorer de nouveaux traitements thérapeutiques basés sur l'immunothérapie adoptive à lymphocytes T. De plus, les complexes spécifiques CMH/peptide peuvent induire ou stimuler des lymphocytes T peptide-spécifiques. Ainsi, on décrit également des méthodes destinées à induire des lymphocytes T cytotoxiques (CTL). Ces CTL peuvent être utilisés à leur tour pour combattre la croissance des cellules tumorales in vitro ou in vivo. La molécule CMH recombinante chargée avec un seul peptide peut être utilisée avec efficacité sous forme polymère pour stimuler une réponse immune spécifique ex vivo ou in vivo après injection à l'homme ou l'animal.
PCT/FR1997/000892 1996-05-21 1997-05-21 Methodes d'utilisation de complexes peptide/complexe majeur d'histocompatibilite pour obtenir ou purifier des cellules t antigene-specifiques et pour stimuler des cellules t WO1997044667A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU30365/97A AU3036597A (en) 1996-05-21 1997-05-21 Method for using peptide complexes/major histocompatibility complex to obtain or purify antigen-specific t cells and to stimulate t cells

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US65192596A 1996-05-21 1996-05-21
US08/651,925 1996-05-21

Publications (2)

Publication Number Publication Date
WO1997044667A2 WO1997044667A2 (fr) 1997-11-27
WO1997044667A3 true WO1997044667A3 (fr) 1998-03-19

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PCT/FR1997/000892 WO1997044667A2 (fr) 1996-05-21 1997-05-21 Methodes d'utilisation de complexes peptide/complexe majeur d'histocompatibilite pour obtenir ou purifier des cellules t antigene-specifiques et pour stimuler des cellules t

Country Status (2)

Country Link
AU (1) AU3036597A (fr)
WO (1) WO1997044667A2 (fr)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7541184B2 (en) 2000-02-24 2009-06-02 Invitrogen Corporation Activation and expansion of cells
US7572631B2 (en) 2000-02-24 2009-08-11 Invitrogen Corporation Activation and expansion of T cells
US9404916B2 (en) 2008-09-20 2016-08-02 University College Cardiff Consultants Limited Use of a protein kinase inhibitor to detect immune cells, such as T cells
US9528088B2 (en) 2002-06-28 2016-12-27 Life Technologies Corporation Methods for eliminating at least a substantial portion of a clonal antigen-specific memory T cell subpopulation

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH11507843A (ja) 1996-03-28 1999-07-13 ザ・ジョンズ・ホプキンス・ユニバーシティー タンパク質の可溶性二価および多価ヘテロ二量体類縁体
US6458354B1 (en) 1996-03-28 2002-10-01 The Johns Hopkins University Molecular complexes which modify immune responses
US6268411B1 (en) 1997-09-11 2001-07-31 The Johns Hopkins University Use of multivalent chimeric peptide-loaded, MHC/ig molecules to detect, activate or suppress antigen-specific T cell-dependent immune responses
JP2002524104A (ja) * 1998-09-14 2002-08-06 ラース オースターガード ペダーセン 機能化免疫グロブリンスーパーファミリータンパク質を製造する方法
IL127331A0 (en) * 1998-11-30 1999-09-22 Yeda Res & Dev Peptide-based vaccine for influenza
FR2798128B1 (fr) * 1999-09-06 2001-11-16 Inst Nat Sante Rech Med Moyens de detection et de purification de populations lymphocytaires t cd8+ specifiques de peptides presentes dans le contexte hla
US20040209314A1 (en) 1999-09-06 2004-10-21 Institut National De La Sante Et De La Recherche Medicale France Means for detection and purification of CD8+ T lymphocyte populations specific to peptides presented in the context of HLA
EP1103265A1 (fr) * 1999-11-15 2001-05-30 Leids Universitair Medisch Centrum Cellules T spécifiques de protéines polymorphiques de cellules malignes
EP2336167B1 (fr) * 2001-03-14 2019-05-29 Dako Denmark A/S Nouvelles constructions de molécules MHC, méthodes d'utilisation de ces constructions à des fins de diagnostic et de therapie et utilisations de molécules MHC
CA2357906A1 (fr) * 2001-09-28 2003-03-28 Institut Pasteur Identification de nouveaux epitopes de cd8 de proteines ayant des proprietes therapeutiques et immunologiques pour lutter contre les infections a vih
DE60334250D1 (de) 2003-05-08 2010-10-28 Life Technologies Corp Erzeugung und isolierung antigenspezifischer t-zellen
EP2361930A3 (fr) 2007-03-26 2011-10-26 Dako Denmark A/S Multimeres du complexes peptide-cmh et leur utilisation dans des maladies infectieuses de borrelia
WO2009003493A2 (fr) 2007-07-03 2009-01-08 Dako Denmark A/S Procédés compilés pour analyser et trier des échantillons
US10611818B2 (en) 2007-09-27 2020-04-07 Agilent Technologies, Inc. MHC multimers in tuberculosis diagnostics, vaccine and therapeutics
DK2254592T3 (da) 2008-02-28 2019-09-09 Dako Denmark As MHC-multimerer til Borrelia-diagnostik og sygdom
WO2010009735A2 (fr) 2008-07-23 2010-01-28 Dako Denmark A/S Analyse et réparation combinatoires
US10369204B2 (en) 2008-10-02 2019-08-06 Dako Denmark A/S Molecular vaccines for infectious disease
US11992518B2 (en) 2008-10-02 2024-05-28 Agilent Technologies, Inc. Molecular vaccines for infectious disease
KR20110117112A (ko) * 2008-12-24 2011-10-26 온코세라피 사이언스 가부시키가이샤 Ｃ１ｏｒｆ５９ 펩티드 및 이를 포함하는 백신
US12258373B2 (en) 2018-12-17 2025-03-25 Immudex Aps Panel comprising Borrelia MHC multimers
CN114746152A (zh) 2020-08-23 2022-07-12 应用干细胞有限公司 Hla-f修饰的细胞和方法

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994023067A1 (fr) * 1993-03-31 1994-10-13 Abbott Laboratories Antigenes associes a des tumeurs reconnus par les lymphocytes et utilisations de ces antigenes
WO1995033995A1 (fr) * 1994-06-06 1995-12-14 Gti Procede et trousse pour detecter des anticorps
WO1995035500A1 (fr) * 1994-06-17 1995-12-28 Ludwig Institute For Cancer Research Procedes de production de lymphocytes t cytolytiques specifiques contre un complexe de peptide et de molecule de mhc
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WO1996026962A1 (fr) * 1995-02-28 1996-09-06 The Board Of Trustees Of The Leland Stanford Junior University Complexes antigenes/mhc pour detecter et purifier les lymphocytes t specifiques aux antigenes
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Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994023067A1 (fr) * 1993-03-31 1994-10-13 Abbott Laboratories Antigenes associes a des tumeurs reconnus par les lymphocytes et utilisations de ces antigenes
WO1995033995A1 (fr) * 1994-06-06 1995-12-14 Gti Procede et trousse pour detecter des anticorps
WO1995035500A1 (fr) * 1994-06-17 1995-12-28 Ludwig Institute For Cancer Research Procedes de production de lymphocytes t cytolytiques specifiques contre un complexe de peptide et de molecule de mhc
WO1996005287A1 (fr) * 1994-08-09 1996-02-22 Anergen, Inc. Procede permettant d'isoler des lymphocytes t du sang peripherique
WO1996026962A1 (fr) * 1995-02-28 1996-09-06 The Board Of Trustees Of The Leland Stanford Junior University Complexes antigenes/mhc pour detecter et purifier les lymphocytes t specifiques aux antigenes
WO1996036881A2 (fr) * 1995-05-16 1996-11-21 Cancer Research Campaign Technology Limited Evaluation preliminaire d'inhibiteurs potentiels des interactions entre les recepteurs des lymphocytes t (rlt) et le complexe majeur d'histocompatibilite (cmh)

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F. GODEAU ET AL.: "Purification and ligand binding of a soluble class I major histocompatibility complex molecule consisting of the first three domains of H-2Kd fused to beta2-microglobulin expressed in the baculovirus-insect cell system.", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 267, no. 5, 1992, pages 24223 - 24229, XP002051156 *
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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7541184B2 (en) 2000-02-24 2009-06-02 Invitrogen Corporation Activation and expansion of cells
US7572631B2 (en) 2000-02-24 2009-08-11 Invitrogen Corporation Activation and expansion of T cells
US9528088B2 (en) 2002-06-28 2016-12-27 Life Technologies Corporation Methods for eliminating at least a substantial portion of a clonal antigen-specific memory T cell subpopulation
US9404916B2 (en) 2008-09-20 2016-08-02 University College Cardiff Consultants Limited Use of a protein kinase inhibitor to detect immune cells, such as T cells

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Publication number Publication date
AU3036597A (en) 1997-12-09
WO1997044667A2 (fr) 1997-11-27

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