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WO1997044667A3 - Method for using peptide complexes/major histocompatibility complex to obtain or purify antigen-specific t cells and to stimulate t cells - Google Patents

Method for using peptide complexes/major histocompatibility complex to obtain or purify antigen-specific t cells and to stimulate t cells Download PDF

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Publication number
WO1997044667A3
WO1997044667A3 PCT/FR1997/000892 FR9700892W WO9744667A3 WO 1997044667 A3 WO1997044667 A3 WO 1997044667A3 FR 9700892 W FR9700892 W FR 9700892W WO 9744667 A3 WO9744667 A3 WO 9744667A3
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WO
WIPO (PCT)
Prior art keywords
peptide
specific
lymphocytes
mhc
cells
Prior art date
Application number
PCT/FR1997/000892
Other languages
French (fr)
Other versions
WO1997044667A2 (en
Inventor
Pierre Langlade-Demoyen
Yu-Chun Lone
Philippe Kourilsky
Jean-Pierre Abastado
Original Assignee
Pasteur Institut
Inst Nat Sante Rech Med
Pierre Langlade-Demoyen
Yu-Chun Lone
Philippe Kourilsky
Jean-Pierre Abastado
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pasteur Institut, Inst Nat Sante Rech Med, Pierre Langlade-Demoyen, Yu-Chun Lone, Philippe Kourilsky, Jean-Pierre Abastado filed Critical Pasteur Institut
Priority to AU30365/97A priority Critical patent/AU3036597A/en
Publication of WO1997044667A2 publication Critical patent/WO1997044667A2/en
Publication of WO1997044667A3 publication Critical patent/WO1997044667A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70503—Immunoglobulin superfamily
    • C07K14/70539—MHC-molecules, e.g. HLA-molecules
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • G01N33/56977—HLA or MHC typing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/16011—Orthomyxoviridae
    • C12N2760/16111—Influenzavirus A, i.e. influenza A virus
    • C12N2760/16122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Cell Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Genetics & Genomics (AREA)
  • Food Science & Technology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Oncology (AREA)
  • Hospice & Palliative Care (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Toxicology (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

The invention features methods for using MHC/peptide complexes. The successful peptide-specific T lymphocyte purification by adsorption on a solid support coupled with a high density of MHC recombinant molecules, charged with a single peptide is disclosed. This method has enabled the isolating, purifying and quantifying of tumoral specific T lymphocytes, from peritoneal exudate and tumor-infiltrating lymphocytes. These purified T lymphocyte populations can be further multiplied in vitro. The resulting cells show a superior cytotoxic activity and an enhanced anti-tumoral action by re-injection in the tumor carrier. This novel approach is important for analysing the T lymphocytes repertoire at the peptide specificity level and to improve novel T lymphocyte adoptive immunotherapy based therapeutic treatments. Further, the MHC/peptide specific complexes can induce or stimulate peptide-specific T lymphocytes. Thus are also disclosed methods to induce cytotoxic T lymphocytes (CTL). These CTL can be used in turn to fight tumoral cell growth in vitro or in vivo. The MHC recombinant molecule charged with a single peptide can be effectively used in polymer form to stimulate specific immune response ex vivo or in vivo after being injected in man or animal.
PCT/FR1997/000892 1996-05-21 1997-05-21 Method for using peptide complexes/major histocompatibility complex to obtain or purify antigen-specific t cells and to stimulate t cells WO1997044667A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU30365/97A AU3036597A (en) 1996-05-21 1997-05-21 Method for using peptide complexes/major histocompatibility complex to obtain or purify antigen-specific t cells and to stimulate t cells

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US65192596A 1996-05-21 1996-05-21
US08/651,925 1996-05-21

Publications (2)

Publication Number Publication Date
WO1997044667A2 WO1997044667A2 (en) 1997-11-27
WO1997044667A3 true WO1997044667A3 (en) 1998-03-19

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/FR1997/000892 WO1997044667A2 (en) 1996-05-21 1997-05-21 Method for using peptide complexes/major histocompatibility complex to obtain or purify antigen-specific t cells and to stimulate t cells

Country Status (2)

Country Link
AU (1) AU3036597A (en)
WO (1) WO1997044667A2 (en)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7541184B2 (en) 2000-02-24 2009-06-02 Invitrogen Corporation Activation and expansion of cells
US7572631B2 (en) 2000-02-24 2009-08-11 Invitrogen Corporation Activation and expansion of T cells
US9404916B2 (en) 2008-09-20 2016-08-02 University College Cardiff Consultants Limited Use of a protein kinase inhibitor to detect immune cells, such as T cells
US9528088B2 (en) 2002-06-28 2016-12-27 Life Technologies Corporation Methods for eliminating at least a substantial portion of a clonal antigen-specific memory T cell subpopulation

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6458354B1 (en) 1996-03-28 2002-10-01 The Johns Hopkins University Molecular complexes which modify immune responses
EP0889964A1 (en) 1996-03-28 1999-01-13 The Johns Hopkins University Soluble divalent and multivalent heterodimeric analogs of proteins
US6268411B1 (en) 1997-09-11 2001-07-31 The Johns Hopkins University Use of multivalent chimeric peptide-loaded, MHC/ig molecules to detect, activate or suppress antigen-specific T cell-dependent immune responses
CN1326466A (en) * 1998-09-14 2001-12-12 拉斯·奥斯特加德·佩德森 A method of producing functional immunoglobulin superfamily proteins
IL127331A0 (en) * 1998-11-30 1999-09-22 Yeda Res & Dev Peptide-based vaccine for influenza
US20040209314A1 (en) 1999-09-06 2004-10-21 Institut National De La Sante Et De La Recherche Medicale France Means for detection and purification of CD8+ T lymphocyte populations specific to peptides presented in the context of HLA
FR2798128B1 (en) * 1999-09-06 2001-11-16 Inst Nat Sante Rech Med MEANS OF DETECTION AND PURIFICATION OF T CD8 + LYMPHOCYTE POPULATIONS SPECIFIC TO PEPTIDES PRESENT IN THE HLA CONTEXT
EP1103265A1 (en) * 1999-11-15 2001-05-30 Leids Universitair Medisch Centrum T-cells specific for polymorphic proteins on malignant cells
EP2336167B1 (en) * 2001-03-14 2019-05-29 Dako Denmark A/S MHC molecule constructs and their uses for diagnosis and therapy
CA2357906A1 (en) * 2001-09-28 2003-03-28 Institut Pasteur Identification of new cd8 epitopes from hiv-1 proteins with therapeutical and vaccinal properties against hiv infections
CA2525519A1 (en) 2003-05-08 2004-12-02 Xcyte Therapies, Inc. Generation and isolation of antigen-specific t cells
EP2155782A2 (en) 2007-03-26 2010-02-24 Dako Denmark A/S Mhc peptide complexes and uses thereof in infectious diseases
WO2009003493A2 (en) 2007-07-03 2009-01-08 Dako Denmark A/S Mhc multimers, methods for their generation, labeling and use
EP2197908A2 (en) 2007-09-27 2010-06-23 Dako Denmark A/S Mhc multimers in tuberculosis diagnostics, vaccine and therapeutics
DK2254592T3 (en) 2008-02-28 2019-09-09 Dako Denmark As MHC multimers for Borrelia diagnostics and disease
WO2010009735A2 (en) 2008-07-23 2010-01-28 Dako Denmark A/S Combinatorial analysis and repair
US11992518B2 (en) 2008-10-02 2024-05-28 Agilent Technologies, Inc. Molecular vaccines for infectious disease
US10369204B2 (en) 2008-10-02 2019-08-06 Dako Denmark A/S Molecular vaccines for infectious disease
US8735361B2 (en) * 2008-12-24 2014-05-27 Oncotherapy Science, Inc. C1ORF59 peptides and vaccines including the same
WO2020127222A2 (en) 2018-12-17 2020-06-25 Immudex Aps Panel comprising borrelia mhc multimers
WO2022046675A1 (en) 2020-08-23 2022-03-03 Applied Stemcell, Inc. Hla-f-modified cells and methods

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994023067A1 (en) * 1993-03-31 1994-10-13 Abbott Laboratories Tumor-associated antigens recognized by t cells and the uses of these antigens
WO1995033995A1 (en) * 1994-06-06 1995-12-14 Gti Method and kit for detecting antibodies
WO1995035500A1 (en) * 1994-06-17 1995-12-28 Ludwig Institute For Cancer Research Method for producing cytolytic t cells specific for a complex of peptide and mhc-molecule
WO1996005287A1 (en) * 1994-08-09 1996-02-22 Anergen, Inc. A method for the isolation of t cells from peripheral blood
WO1996026962A1 (en) * 1995-02-28 1996-09-06 The Board Of Trustees Of The Leland Stanford Junior University Mhc-antigen complexes for detecting and purifying antigen-specific t cells
WO1996036881A2 (en) * 1995-05-16 1996-11-21 Cancer Research Campaign Technology Limited Screening for inhibitors of tcr-mhc interactions

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994023067A1 (en) * 1993-03-31 1994-10-13 Abbott Laboratories Tumor-associated antigens recognized by t cells and the uses of these antigens
WO1995033995A1 (en) * 1994-06-06 1995-12-14 Gti Method and kit for detecting antibodies
WO1995035500A1 (en) * 1994-06-17 1995-12-28 Ludwig Institute For Cancer Research Method for producing cytolytic t cells specific for a complex of peptide and mhc-molecule
WO1996005287A1 (en) * 1994-08-09 1996-02-22 Anergen, Inc. A method for the isolation of t cells from peripheral blood
WO1996026962A1 (en) * 1995-02-28 1996-09-06 The Board Of Trustees Of The Leland Stanford Junior University Mhc-antigen complexes for detecting and purifying antigen-specific t cells
WO1996036881A2 (en) * 1995-05-16 1996-11-21 Cancer Research Campaign Technology Limited Screening for inhibitors of tcr-mhc interactions

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
F. GODEAU ET AL.: "Purification and ligand binding of a soluble class I major histocompatibility complex molecule consisting of the first three domains of H-2Kd fused to beta2-microglobulin expressed in the baculovirus-insect cell system.", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 267, no. 5, 1992, pages 24223 - 24229, XP002051156 *
TOSHITANI, KOJI ET AL: "Expression of a single chain HLA class I molecule in a human cell line: presentation of exogenous peptide and processed antigen to cytotoxic T lymphocytes", PROC. NATL. ACAD. SCI. U. S. A. (1996), 93(1), 236-40 CODEN: PNASA6;ISSN: 0027-8424, XP002051157 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7541184B2 (en) 2000-02-24 2009-06-02 Invitrogen Corporation Activation and expansion of cells
US7572631B2 (en) 2000-02-24 2009-08-11 Invitrogen Corporation Activation and expansion of T cells
US9528088B2 (en) 2002-06-28 2016-12-27 Life Technologies Corporation Methods for eliminating at least a substantial portion of a clonal antigen-specific memory T cell subpopulation
US9404916B2 (en) 2008-09-20 2016-08-02 University College Cardiff Consultants Limited Use of a protein kinase inhibitor to detect immune cells, such as T cells

Also Published As

Publication number Publication date
AU3036597A (en) 1997-12-09
WO1997044667A2 (en) 1997-11-27

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