WO1996034840A1 - Engrais bacterien et son procede d'obtention - Google Patents
Engrais bacterien et son procede d'obtention Download PDFInfo
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- WO1996034840A1 WO1996034840A1 PCT/ES1996/000097 ES9600097W WO9634840A1 WO 1996034840 A1 WO1996034840 A1 WO 1996034840A1 ES 9600097 W ES9600097 W ES 9600097W WO 9634840 A1 WO9634840 A1 WO 9634840A1
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- 239000003337 fertilizer Substances 0.000 title claims abstract description 21
- 230000001580 bacterial effect Effects 0.000 title claims abstract description 14
- 238000004519 manufacturing process Methods 0.000 title description 12
- 244000005700 microbiome Species 0.000 claims abstract description 26
- 238000000034 method Methods 0.000 claims abstract description 11
- 241000589938 Azospirillum brasilense Species 0.000 claims abstract description 7
- 241000589149 Azotobacter vinelandii Species 0.000 claims abstract description 7
- 239000000725 suspension Substances 0.000 claims abstract description 6
- WHUUTDBJXJRKMK-UHFFFAOYSA-N glutamic acid Chemical compound OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 claims description 22
- 239000002609 medium Substances 0.000 claims description 21
- 229930006000 Sucrose Natural products 0.000 claims description 20
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 20
- 239000005720 sucrose Substances 0.000 claims description 20
- 239000001963 growth medium Substances 0.000 claims description 15
- 238000011081 inoculation Methods 0.000 claims description 13
- PYMYPHUHKUWMLA-LMVFSUKVSA-N aldehydo-D-ribose Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)C=O PYMYPHUHKUWMLA-LMVFSUKVSA-N 0.000 claims description 6
- 230000012010 growth Effects 0.000 claims description 6
- 239000002054 inoculum Substances 0.000 claims description 6
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 claims description 5
- 229930195725 Mannitol Natural products 0.000 claims description 5
- 238000012258 culturing Methods 0.000 claims description 5
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 claims description 5
- 239000000594 mannitol Substances 0.000 claims description 5
- 235000010355 mannitol Nutrition 0.000 claims description 5
- 235000015393 sodium molybdate Nutrition 0.000 claims description 5
- 239000011684 sodium molybdate Substances 0.000 claims description 5
- TVXXNOYZHKPKGW-UHFFFAOYSA-N sodium molybdate (anhydrous) Chemical compound [Na+].[Na+].[O-][Mo]([O-])(=O)=O TVXXNOYZHKPKGW-UHFFFAOYSA-N 0.000 claims description 5
- 238000011534 incubation Methods 0.000 claims description 4
- 238000007747 plating Methods 0.000 claims description 4
- 241000894006 Bacteria Species 0.000 claims description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 3
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 claims description 3
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 claims description 3
- 238000005273 aeration Methods 0.000 claims description 2
- 239000012736 aqueous medium Substances 0.000 claims description 2
- 239000005373 porous glass Substances 0.000 claims description 2
- 238000002360 preparation method Methods 0.000 claims description 2
- 238000005507 spraying Methods 0.000 claims description 2
- 241000196324 Embryophyta Species 0.000 abstract description 17
- 210000000416 exudates and transudate Anatomy 0.000 abstract description 4
- 230000008901 benefit Effects 0.000 abstract description 3
- 150000001875 compounds Chemical class 0.000 abstract description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract 1
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 26
- 229910052757 nitrogen Inorganic materials 0.000 description 13
- 239000002689 soil Substances 0.000 description 13
- 235000010443 alginic acid Nutrition 0.000 description 11
- 229920000615 alginic acid Polymers 0.000 description 11
- 239000000758 substrate Substances 0.000 description 11
- FHVDTGUDJYJELY-UHFFFAOYSA-N 6-{[2-carboxy-4,5-dihydroxy-6-(phosphanyloxy)oxan-3-yl]oxy}-4,5-dihydroxy-3-phosphanyloxane-2-carboxylic acid Chemical compound O1C(C(O)=O)C(P)C(O)C(O)C1OC1C(C(O)=O)OC(OP)C(O)C1O FHVDTGUDJYJELY-UHFFFAOYSA-N 0.000 description 7
- 240000008042 Zea mays Species 0.000 description 7
- 229940072056 alginate Drugs 0.000 description 7
- 230000015572 biosynthetic process Effects 0.000 description 7
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 6
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 5
- 241000589151 Azotobacter Species 0.000 description 4
- 240000007594 Oryza sativa Species 0.000 description 4
- 235000007164 Oryza sativa Nutrition 0.000 description 4
- 241000209140 Triticum Species 0.000 description 4
- 235000021307 Triticum Nutrition 0.000 description 4
- 235000013339 cereals Nutrition 0.000 description 4
- 229920001282 polysaccharide Polymers 0.000 description 4
- 239000005017 polysaccharide Substances 0.000 description 4
- 150000004804 polysaccharides Chemical class 0.000 description 4
- 235000009566 rice Nutrition 0.000 description 4
- 229930192334 Auxin Natural products 0.000 description 3
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 description 3
- 239000002363 auxin Substances 0.000 description 3
- 239000004202 carbamide Substances 0.000 description 3
- 238000011161 development Methods 0.000 description 3
- 230000018109 developmental process Effects 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 235000009973 maize Nutrition 0.000 description 3
- 229930195732 phytohormone Natural products 0.000 description 3
- FJKROLUGYXJWQN-UHFFFAOYSA-N 4-hydroxybenzoic acid Chemical compound OC(=O)C1=CC=C(O)C=C1 FJKROLUGYXJWQN-UHFFFAOYSA-N 0.000 description 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- 235000021537 Beetroot Nutrition 0.000 description 2
- 239000002028 Biomass Substances 0.000 description 2
- 240000007124 Brassica oleracea Species 0.000 description 2
- 235000003899 Brassica oleracea var acephala Nutrition 0.000 description 2
- 235000011301 Brassica oleracea var capitata Nutrition 0.000 description 2
- 235000001169 Brassica oleracea var oleracea Nutrition 0.000 description 2
- 244000000626 Daucus carota Species 0.000 description 2
- 235000002767 Daucus carota Nutrition 0.000 description 2
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 2
- 229910019142 PO4 Inorganic materials 0.000 description 2
- 240000003768 Solanum lycopersicum Species 0.000 description 2
- 244000062793 Sorghum vulgare Species 0.000 description 2
- 235000007244 Zea mays Nutrition 0.000 description 2
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 235000005822 corn Nutrition 0.000 description 2
- 239000004062 cytokinin Substances 0.000 description 2
- UQHKFADEQIVWID-UHFFFAOYSA-N cytokinin Natural products C1=NC=2C(NCC=C(CO)C)=NC=NC=2N1C1CC(O)C(CO)O1 UQHKFADEQIVWID-UHFFFAOYSA-N 0.000 description 2
- 235000021186 dishes Nutrition 0.000 description 2
- 230000002349 favourable effect Effects 0.000 description 2
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 description 2
- 239000002207 metabolite Substances 0.000 description 2
- 239000000618 nitrogen fertilizer Substances 0.000 description 2
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 2
- 235000021317 phosphate Nutrition 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 239000010902 straw Substances 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- 230000002194 synthesizing effect Effects 0.000 description 2
- XGILAAMKEQUXLS-UHFFFAOYSA-N 3-(indol-3-yl)lactic acid Chemical compound C1=CC=C2C(CC(O)C(O)=O)=CNC2=C1 XGILAAMKEQUXLS-UHFFFAOYSA-N 0.000 description 1
- 229940090248 4-hydroxybenzoic acid Drugs 0.000 description 1
- 125000005274 4-hydroxybenzoic acid group Chemical group 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 235000007319 Avena orientalis Nutrition 0.000 description 1
- 244000075850 Avena orientalis Species 0.000 description 1
- 241000589941 Azospirillum Species 0.000 description 1
- 241000206672 Gelidium Species 0.000 description 1
- 241001154602 Gmelina arborea Species 0.000 description 1
- 240000005979 Hordeum vulgare Species 0.000 description 1
- 235000007340 Hordeum vulgare Nutrition 0.000 description 1
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 1
- 235000004431 Linum usitatissimum Nutrition 0.000 description 1
- 240000006240 Linum usitatissimum Species 0.000 description 1
- 240000000249 Morus alba Species 0.000 description 1
- 235000008708 Morus alba Nutrition 0.000 description 1
- -1 NH4+ ion Chemical class 0.000 description 1
- 244000061176 Nicotiana tabacum Species 0.000 description 1
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 1
- 108010020943 Nitrogenase Proteins 0.000 description 1
- 240000004713 Pisum sativum Species 0.000 description 1
- 235000010582 Pisum sativum Nutrition 0.000 description 1
- 241000589180 Rhizobium Species 0.000 description 1
- 240000000111 Saccharum officinarum Species 0.000 description 1
- 235000007201 Saccharum officinarum Nutrition 0.000 description 1
- 239000000589 Siderophore Substances 0.000 description 1
- 244000061456 Solanum tuberosum Species 0.000 description 1
- 235000002595 Solanum tuberosum Nutrition 0.000 description 1
- 235000011684 Sorghum saccharatum Nutrition 0.000 description 1
- 241000131774 Tectona Species 0.000 description 1
- 241000209149 Zea Species 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 235000010419 agar Nutrition 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 239000000783 alginic acid Substances 0.000 description 1
- 229960001126 alginic acid Drugs 0.000 description 1
- 150000004781 alginic acids Chemical class 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000000843 anti-fungal effect Effects 0.000 description 1
- 229940121375 antifungal agent Drugs 0.000 description 1
- 238000003287 bathing Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 235000020776 essential amino acid Nutrition 0.000 description 1
- 239000003797 essential amino acid Substances 0.000 description 1
- 230000004720 fertilization Effects 0.000 description 1
- 239000002803 fossil fuel Substances 0.000 description 1
- 238000001879 gelation Methods 0.000 description 1
- 230000035784 germination Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000003102 growth factor Substances 0.000 description 1
- 239000003630 growth substance Substances 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
- 229930182817 methionine Natural products 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 244000000010 microbial pathogen Species 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 235000019713 millet Nutrition 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 235000013379 molasses Nutrition 0.000 description 1
- 244000138993 panchioli Species 0.000 description 1
- 230000002572 peristaltic effect Effects 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 150000003013 phosphoric acid derivatives Chemical class 0.000 description 1
- 230000008121 plant development Effects 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 230000003389 potentiating effect Effects 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/065—Azotobacter
Definitions
- the object of the present invention is a bacterial fertilizer consisting of a suspension containing cells of new strains of the microorganisms Rzotobacter and Azo ⁇ plrillum. Said microorganisms and the procedure for obtaining said bacterial fertilizer also constitute another object of the present invention.
- the main atmospheric nitrogen-fixing microorganisms that have been used as bacterial fertilizers are Rzotobacter and Azo ⁇ pirllum.
- Rzotobacter has been used as a nitrogenous fertilizer in some plants of agricultural interest such as corn, wheat, potato, beetroot, tobacco, barley, flax, oats, carrots, cabbage and tomato. Inoculation with Rzotobacter as a bacterial fertilizer has been developed for many years (Rubenchic L.I., "Azotobacter and Its Use in Agriculture". Academy of Sciences of the Ukrainian SSR. Microbiological Institute im. D.K. Zabolotnyi, 1963). Of all the strains, the most used as inoculant has been R. chroococcum, as it is the most frequent and abundant in the soil (Krieg N.R, Holt, J.G., Williams and Wilkins Co., Baltimore, 1984).
- the favorable effect of the inoculation with Azotoiacter and Rzo ⁇ pirillum cells on different agricultural crops is the product of a multiple action because it represents an alteration of the suppressive microbial balance of pathogenic microorganisms, mobilization of phosphates, assimilation of exudates from the roots of the plants (acid p -hydroxybenzoic, ... ) and production of metabolites such as phytohormones that stimulate growth before and after germination, also representing a very considerable saving in nitrogen fertilizers due to the fixation of N 2 in the soil.
- Rzotobacter and Rzo ⁇ pirillum are related not only to their ability to fix N 2 but also to the ability to produce antibacterial and antifungal compounds, growth regulators, and siderophores (Pandey, A.; "Potential of Azotobacters and Azospirilla as biofertilizers for upland agriculture: a review”; Journal of Scientific and Industrial Research 48.3, 134-144, 1989).
- the saving of nitrogenous fertilizers supposes a parallel saving of fossil fuels.
- the object of the present invention is a bacterial fertilizer that contains cells of new strains of the microorganisms Rzotobacter and Rzo ⁇ pirillum specifically developed to improve N 2 fixation and potentiate alginate biosynthesis. .
- aqueous medium Purk medium
- the microorganisms suspended in said medium are a strain of Rzotobacter vinelandii deposited in the Spanish Type Culture Collection (CECT) with the number CECT 4534 and a strain of Rzo ⁇ pirillum bra ⁇ ilen ⁇ e deposited in the Spanish Collection of Type Cultures with the number CECT 4533.
- CECT Type Culture Collection
- Rzotobacter and Rzo ⁇ pirillum cells By subjecting Rzotobacter and Rzo ⁇ pirillum cells in liquid and solid cultures (with agar-agar) to different substrates, variants of these strains capable of fixing more atmospheric nitrogen, synthesizing more phytohormones (cytokinins, auxins, . . . ) and metabolites such as alginates and essential amino acids, such as usine and methionine, in higher concentration.
- phytohormones cytokinins, auxins, . . .
- metabolites such as alginates and essential amino acids, such as usine and methionine
- strains also present a greater facility for using waste products as substrates, for example, the exudates of the roots of the plants (ortho-, meta- and parahydroxybenzoic acids,%) and agricultural residues such as molasses, vine shoots, stillage, etc
- waste products for example, the exudates of the roots of the plants (ortho-, meta- and parahydroxybenzoic acids,...) and agricultural residues such as molasses, vine shoots, stillage, etc
- alginates allows these Rzotobacter and Rzo ⁇ pirillum cells to better adhere to plant roots and at the same time have this capsular substrate available for growth and reproduction in the soil.
- the contribution of DL glutamic acid is intended to inhibit the nitrogenase enzyme to enhance alginate biosynthesis.
- cells are obtained, isolated on plates, which in subsequent standardized cultures, with sucrose as a substrate, reach a production of 2.4 g/1 of alginate with a high index of uronic acids (the basis for good gelation). These cells are designated by the initials 382 12 E of R . vinelandii . At the same time that they synthesize a greater amount of alginate, they fix a higher N 2 concentration than the original strains.
- the strains of Rzo ⁇ pirillum bra ⁇ ilen ⁇ e obtained from the 590 CECT strain are prepared by the same procedure as in the case of Rzotobacter , but with D-Ribose as the only substrate in all steps, obtaining a strain called Rzo ⁇ pirillum with the acronym 40AM.
- bra ⁇ ilen ⁇ e that has been deposited in the CECT having been assigned the number CECT 4533.
- the ideal culture medium for the best development of Rzotobacter and Rzo ⁇ pirillum cells is the following:
- the final pH of the culture medium is adjusted to 7.4.
- the strains of AzotoJ acter vinelandii and Rzo ⁇ pirillum bra ⁇ ilen ⁇ e are seeded on solid media in Petri dishes and incubated at 30°C for a period of time between 72 and 96 hours. These freshly grown, pure cultures are used to inoculate 300 ml flasks with 100 ml of culture medium, which are incubated at a temperature between 30 and 32°C for 120 hours at 175 revolutions per minute in an orbital incubator.
- the cell biomass of the mixture of Rzotobacter and Rzo ⁇ pirillum is then available to be inoculated into the seeds and subsequently into the soil.
- Rzotobacter and Rzo ⁇ pirillum cells will serve the Rzotobacter and Rzo ⁇ pirillum cells as an energy reserve for their subsequent development in the soil, at the same time that they will allow them to adhere more easily to the roots of the plants. 3.- These variants of Rzotobacter and Rzo ⁇ pirillum also have a greater capacity to assimilate the compounds formed by the exudates of the roots of the plants, such as p-hydroxybenzoic acid, which is hardly used by other strains.
- the speed of reproduction and assimilation of the substrates is also higher in the Rzotobacter and Rzo ⁇ pirillum variants than in the known strains, since the maximum dry weight reached by them is up to 3 times higher after 52 hours of culture. submerged at about 28-30°C. 5.- The performance and effectiveness of these Rzotobacter variants is much higher than the known strains and the initial strain 382 DSM, also synthesizing polysaccharides with a high gelling index (alginates).
- the cell biomass production of the Rzotobacter strain 24 APV is of the order of 4 g/1 dry weight of lyophilized cells.
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicinal Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Virology (AREA)
- Fertilizers (AREA)
Abstract
La présente invention concerne un engrais bactérien (bioengrais) consistant en une suspension qui contient des cellules de nouvelles souches des microorganismes Azotobacter vinelandii et Azospirilum brasilense. Cet engrais bactérien consiste en une suspension de micro-organismes dans un milieu aqueux. Les micro-organismes sont obtenus par un procédé qui comprend: a) la culture en plaques de la souche originale; b) le traitement des colonies précédentes de la culture antérieure avec un autre milieu de culture; et c) la culture des colonies isolées de l'étape antérieure. Les avantages qui découlent de l'utilisation de ces nouvelles souches de micro-organismes sont leur plus grande tolérance à des milieux très alcalins et une meilleure fixation de N2, ainsi qu'une plus grande capacité d'assimilation des composés formés par les exsudats des racines des plantes.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ESP9500851 | 1995-05-04 | ||
| ES09500851A ES2093559B1 (es) | 1995-05-04 | 1995-05-04 | Fertilizante bacteriano y procedimiento de obtencion. |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| WO1996034840A1 true WO1996034840A1 (fr) | 1996-11-07 |
Family
ID=8290254
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/ES1996/000097 WO1996034840A1 (fr) | 1995-05-04 | 1996-04-29 | Engrais bacterien et son procede d'obtention |
Country Status (2)
| Country | Link |
|---|---|
| ES (1) | ES2093559B1 (fr) |
| WO (1) | WO1996034840A1 (fr) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1998037038A3 (fr) * | 1997-02-25 | 1998-11-12 | Arpad Pollak | Procede pour extraire l'azote de l'air, pour extraire les composes de phosphore du sol et pour decomposer les sediments d'huile |
| WO2001032587A1 (fr) * | 1999-11-01 | 2001-05-10 | 'piksa Inter' Ltd | Produit d'addition biologique pour fertilisants organomineraux |
| WO2001085916A1 (fr) * | 2000-05-12 | 2001-11-15 | Biofarmtech Co., Ltd. | Materiau de revetement photocatalytique presentant une activite photocatalytique et des proprietes d'adsorption, et procede de preparation associe |
| WO2003016241A1 (fr) * | 2001-08-13 | 2003-02-27 | Agro.Bio Hungary Kft. | Micro-organismes pour le traitement du sol et son procede de production |
| EP1135462A4 (fr) * | 1998-12-10 | 2004-07-14 | Tatko Biotech Inc | Micro-organismes pour augmenter la productivite vegetale |
| CN100376666C (zh) * | 2006-03-01 | 2008-03-26 | 华南农业大学 | 糖蜜草固氮螺菌及其应用 |
| EP2227936A1 (fr) * | 2009-03-09 | 2010-09-15 | Pierre-Philippe Claude | Biofertilisation de grandes cultures à l'aide de bactéries azotobacteraceae ayant sur-accumlé du molybdène |
| CN103159512A (zh) * | 2011-12-08 | 2013-06-19 | 邵阳市新瑞生物科技有限公司 | 一种富硒动物蛋白生物有机肥及其制备方法 |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2007358190B2 (en) | 2007-08-27 | 2013-09-12 | Probelte, S.A. | Novel biological fertiliser, method for obtaining same and use thereof as a plant growth stimulator |
| ES2378040B1 (es) | 2010-03-31 | 2013-02-18 | Probelte, S.A | Un preparado biológico bionematicida y estimulador del crecimiento vegetal y cultivos puros de las cepas denominadas n11, sr11 y alo1, contenidas en el mismo. |
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| WO1984001686A1 (fr) * | 1982-10-28 | 1984-05-10 | Biotech General Corp | Nouvelles souches d'azospirillum, procedes de culture de ces souches, compositions les contenant et leur utilisation en tant que bioengrais |
| EP0241583A2 (fr) * | 1986-04-08 | 1987-10-21 | Zhenhua Yang | Azotobacter antiammoniac et son utilisation |
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| ES8706826A1 (es) * | 1986-05-07 | 1987-07-01 | Consejo Superior Investigacion | Procedimiento de obtencion de un mutante de azotobacter vinelandii capaz de fijar n2 en el suelo en concentraciones superiores a las estirpes silvestres conocidas |
| ES2041219B1 (es) * | 1992-04-09 | 1994-06-01 | Consejo Superior Investigacion | Procedimiento de activacion microbiologica en la fijacion de nitrogeno. |
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1995
- 1995-05-04 ES ES09500851A patent/ES2093559B1/es not_active Expired - Fee Related
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- 1996-04-29 WO PCT/ES1996/000097 patent/WO1996034840A1/fr unknown
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| WO1984001686A1 (fr) * | 1982-10-28 | 1984-05-10 | Biotech General Corp | Nouvelles souches d'azospirillum, procedes de culture de ces souches, compositions les contenant et leur utilisation en tant que bioengrais |
| EP0241583A2 (fr) * | 1986-04-08 | 1987-10-21 | Zhenhua Yang | Azotobacter antiammoniac et son utilisation |
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| Title |
|---|
| COMMUN. SOIL SCI. PLANT ANAL., Vol. 24 (3 y 4), 1993, MARTIN et al., "Azotobacter and Azospirillum as Potential Nitrogen Fertilizers", pages 255-260. * |
| FOLIA MICROBIOL., Vol. 35, 1990, FAYEZ M., "Untraditional N2-Fixing Bacteria as Biofertilizers for Wheat and Barley", pages 218-226. * |
| JOURNAL OF SCIENTIFIC & INDUSTRIAL RESEARCH, Vol. 48, 1989, PANDEY A. & KUMAR S., "Potential of Azotobacters and Azospirilla as Biofertilizers for Upland Agriculture: a Review", pages 134-144. * |
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|---|---|---|---|---|
| CZ299601B6 (cs) * | 1997-02-25 | 2008-09-17 | Zpusob prípravy smesi mikroorganismu na vázání dusíku ze vzduchu, na zvyšování rozpustnosti sloucenin fosforu a na rozklad potravinárského olejového sedimentu | |
| WO1998037038A3 (fr) * | 1997-02-25 | 1998-11-12 | Arpad Pollak | Procede pour extraire l'azote de l'air, pour extraire les composes de phosphore du sol et pour decomposer les sediments d'huile |
| EP1135462A4 (fr) * | 1998-12-10 | 2004-07-14 | Tatko Biotech Inc | Micro-organismes pour augmenter la productivite vegetale |
| WO2001032587A1 (fr) * | 1999-11-01 | 2001-05-10 | 'piksa Inter' Ltd | Produit d'addition biologique pour fertilisants organomineraux |
| EP1248754A4 (fr) * | 1999-11-01 | 2003-03-12 | Vinarov Alexandr Juryievich | Produit d'addition biologique pour fertilisants organomineraux |
| US6939688B1 (en) | 1999-11-01 | 2005-09-06 | Soil Biogenics Ltd | Biological addition to organic-mineral fertilizers |
| WO2001085916A1 (fr) * | 2000-05-12 | 2001-11-15 | Biofarmtech Co., Ltd. | Materiau de revetement photocatalytique presentant une activite photocatalytique et des proprietes d'adsorption, et procede de preparation associe |
| US7842494B2 (en) | 2001-08-13 | 2010-11-30 | Agro.Bio Hungary Kft. | Micro-organisms for the treatment of soil and process for obtaining them |
| EA009126B1 (ru) * | 2001-08-13 | 2007-10-26 | Агро. Био Хангери Кфт. | Микроорганизмы для обработки почвы и способ их получения |
| KR100940615B1 (ko) * | 2001-08-13 | 2010-02-05 | 아그로.바이오 헝가리 케이에프티. | 토양 처리용 미생물 및 이를 얻기 위한 방법 |
| WO2003016241A1 (fr) * | 2001-08-13 | 2003-02-27 | Agro.Bio Hungary Kft. | Micro-organismes pour le traitement du sol et son procede de production |
| EP2233458A3 (fr) * | 2001-08-13 | 2011-02-23 | Agro.Bio Hungary Kft. | Microorganismes pour le traitement du sol et un procédé de leur obtention |
| HRP20040126B1 (hr) * | 2001-08-13 | 2014-02-14 | Agro-Bio Hungary Kft. | Mikroorganizmi za tretiranje tla i postupci za njihovo dobivanje |
| CN100376666C (zh) * | 2006-03-01 | 2008-03-26 | 华南农业大学 | 糖蜜草固氮螺菌及其应用 |
| EP2227936A1 (fr) * | 2009-03-09 | 2010-09-15 | Pierre-Philippe Claude | Biofertilisation de grandes cultures à l'aide de bactéries azotobacteraceae ayant sur-accumlé du molybdène |
| CN103159512A (zh) * | 2011-12-08 | 2013-06-19 | 邵阳市新瑞生物科技有限公司 | 一种富硒动物蛋白生物有机肥及其制备方法 |
Also Published As
| Publication number | Publication date |
|---|---|
| ES2093559A1 (es) | 1996-12-16 |
| ES2093559B1 (es) | 1997-07-01 |
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