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WO1995006764A3 - Oligonucleotides with rna cleavage activity - Google Patents

Oligonucleotides with rna cleavage activity Download PDF

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Publication number
WO1995006764A3
WO1995006764A3 PCT/IB1994/000288 IB9400288W WO9506764A3 WO 1995006764 A3 WO1995006764 A3 WO 1995006764A3 IB 9400288 W IB9400288 W IB 9400288W WO 9506764 A3 WO9506764 A3 WO 9506764A3
Authority
WO
WIPO (PCT)
Prior art keywords
oligonucleotides
cleavage activity
rna cleavage
rna
activity
Prior art date
Application number
PCT/IB1994/000288
Other languages
French (fr)
Other versions
WO1995006764A2 (en
Inventor
Larry W Mclaughlin
Dong-Jing Fu
Fritz Benseler
Gerd Kotzorek
Janos Ludwig
Original Assignee
Ribonetics Gmbh
Larry W Mclaughlin
Fu Dong Jing
Fritz Benseler
Gerd Kotzorek
Janos Ludwig
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ribonetics Gmbh, Larry W Mclaughlin, Fu Dong Jing, Fritz Benseler, Gerd Kotzorek, Janos Ludwig filed Critical Ribonetics Gmbh
Priority to AU76231/94A priority Critical patent/AU7623194A/en
Publication of WO1995006764A2 publication Critical patent/WO1995006764A2/en
Publication of WO1995006764A3 publication Critical patent/WO1995006764A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1135—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against oncogenes or tumor suppressor genes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/12—Type of nucleic acid catalytic nucleic acids, e.g. ribozymes
    • C12N2310/121—Hammerhead
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/31—Chemical structure of the backbone
    • C12N2310/318—Chemical structure of the backbone where the PO2 is completely replaced, e.g. MMI or formacetal
    • C12N2310/3183—Diol linkers, e.g. glycols or propanediols
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/32—Chemical structure of the sugar
    • C12N2310/322—2'-R Modification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/35—Nature of the modification
    • C12N2310/352—Nature of the modification linked to the nucleic acid via a carbon atom
    • C12N2310/3527—Other alkyl chain
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/35—Nature of the modification
    • C12N2310/353—Nature of the modification linked to the nucleic acid via an atom other than carbon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/35—Nature of the modification
    • C12N2310/353—Nature of the modification linked to the nucleic acid via an atom other than carbon
    • C12N2310/3533—Halogen
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/35—Nature of the modification
    • C12N2310/353—Nature of the modification linked to the nucleic acid via an atom other than carbon
    • C12N2310/3535—Nitrogen

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Oncology (AREA)
  • Saccharide Compounds (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

The present invention provides novel oligonucleotides, including oligonucleotides with RNA cleavage activity.
PCT/IB1994/000288 1993-09-03 1994-09-05 Oligonucleotides with rna cleavage activity WO1995006764A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU76231/94A AU7623194A (en) 1993-09-03 1994-09-05 Oligonucleotides with rna cleavage activity

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US11629193A 1993-09-03 1993-09-03
US08/116,291 1993-09-03

Publications (2)

Publication Number Publication Date
WO1995006764A2 WO1995006764A2 (en) 1995-03-09
WO1995006764A3 true WO1995006764A3 (en) 1995-10-19

Family

ID=22366298

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB1994/000288 WO1995006764A2 (en) 1993-09-03 1994-09-05 Oligonucleotides with rna cleavage activity

Country Status (2)

Country Link
AU (1) AU7623194A (en)
WO (1) WO1995006764A2 (en)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5998193A (en) * 1994-06-24 1999-12-07 Gene Shears Pty., Ltd. Ribozymes with optimized hybridizing arms, stems, and loops, tRNA embedded ribozymes and compositions thereof
US5716824A (en) * 1995-04-20 1998-02-10 Ribozyme Pharmaceuticals, Inc. 2'-O-alkylthioalkyl and 2-C-alkylthioalkyl-containing enzymatic nucleic acids (ribozymes)
US6277634B1 (en) * 1995-06-07 2001-08-21 Commonwealth Scientific And Industrial Research Organization Optimized minizymes and miniribozymes and uses thereof
US6127159A (en) 1997-06-06 2000-10-03 The Board Of Trustees Of The Leland Stanford Junior University Mitofusin genes and their uses
US6270747B1 (en) 1998-08-18 2001-08-07 The University Of California In vitro and in vivo assay for agents which treat mucus hypersecretion
US6379931B1 (en) * 1999-08-12 2002-04-30 City Of Hope Chimeric DNA/RNA ribozymes containing propanediol
AU8003400A (en) 1999-10-06 2001-05-10 Ansgar H. Santel Mitofusins, fzo homologs and functional derivatives thereof
AU785425B2 (en) 2001-03-30 2007-05-17 Genetic Technologies Limited Methods of genomic analysis
DK1465634T3 (en) 2001-12-12 2015-01-26 Us Government Method of Using Adenosine Receptor Inhibitors to Boost Immune Response and Inflammation
US7351542B2 (en) 2002-05-20 2008-04-01 The Regents Of The University Of California Methods of modulating tubulin deacetylase activity
CA2557426A1 (en) 2004-02-24 2005-10-06 Thomas W. Hodge Rab9a, rab11a, and modulators thereof related to infectious disease
US20090087854A1 (en) 2007-09-27 2009-04-02 Perlegen Sciences, Inc. Methods for genetic analysis
SG156690A1 (en) 2004-10-27 2009-11-26 Univ Vanderbilt Mammalian genes involved in infection
US8106013B2 (en) 2006-05-19 2012-01-31 Georgia Tech Research Corporation ABC transporter ligand GATX1
US8324158B2 (en) 2006-07-14 2012-12-04 Georgia Tech Research Corporation Methods for inhibiting CLC-2 channel with GATX2
WO2016187217A2 (en) 2015-05-18 2016-11-24 The Board Of Trustees Of The Leland Stanford Junior University Methods and compositions for treating aging-associated impairments
US10202615B2 (en) 2010-12-10 2019-02-12 Vanderbilt University Mammalian genes involved in toxicity and infection
US10265372B2 (en) 2014-08-12 2019-04-23 The Regents Of The University Of California Molecular composition for enhancing and rejuvenating maintenance and repair of mammalian tissues

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992007065A1 (en) * 1990-10-12 1992-04-30 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Modified ribozymes
WO1993015187A1 (en) * 1992-01-31 1993-08-05 Massachusetts Institute Of Technology Nucleozymes
WO1994013688A1 (en) * 1992-12-08 1994-06-23 Gene Shears Pty. Limited Dna-armed ribozymes and minizymes
WO1994013789A2 (en) * 1992-12-04 1994-06-23 Ribonetics Gmbh Oligonucleotides with rna cleavage activity
WO1994013793A1 (en) * 1992-12-04 1994-06-23 Apollon, Inc. Compounds and methods for the treatment of leukemias

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992007065A1 (en) * 1990-10-12 1992-04-30 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Modified ribozymes
WO1993015187A1 (en) * 1992-01-31 1993-08-05 Massachusetts Institute Of Technology Nucleozymes
WO1994013789A2 (en) * 1992-12-04 1994-06-23 Ribonetics Gmbh Oligonucleotides with rna cleavage activity
WO1994013793A1 (en) * 1992-12-04 1994-06-23 Apollon, Inc. Compounds and methods for the treatment of leukemias
WO1994013688A1 (en) * 1992-12-08 1994-06-23 Gene Shears Pty. Limited Dna-armed ribozymes and minizymes

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
D. B. OLSEN ET AL.: "Study of a hammerhead ribozyme containing 2'-modified adenosine residues", BIOCHEMISTRY, vol. 30, pages 9735 - 41 *
D. M. WILLIAMS ET AL.: "Function of specific 2'-hydroxyl groups of guanosines in a hammerhead ribozyme probed by 2'-modifications", P.N.A.S., vol. 89, pages 918 - 21 *
F. BENSELER ET AL.: "Hammerhead-like molecules containing non-nucleoside linkers are active RNA catalysts", J. AM. CHEM. SOC., vol. 115, pages 8483 - 4 *
M. Y.-X. MA ET AL.: "Design and Synthesis of RNA miniduplexes via a synthetic linker approach", BIOCHEMISTRY, vol. 32, pages 1751 - 58 *
O. HEIDENREICH, F. ECKSTEIN: "Hammerhead ribozyme-mediated cleavage of the long terminal repeat RNA of human immunodeficiency virus type I", J. BIOL. CHEM., vol. 267, pages 1904 - 9 *
W. A. PIEKEN ET AL.: "Kinetic characterization of ribonuclease-resistant 2'-modified hammerhead-ribozymes", SCIENCE, vol. 253, pages 314 - 7 *

Also Published As

Publication number Publication date
AU7623194A (en) 1995-03-22
WO1995006764A2 (en) 1995-03-09

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