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WO1994029472A3 - Mengovirus utilise comme vecteur d'expression de polypeptides etrangers - Google Patents

Mengovirus utilise comme vecteur d'expression de polypeptides etrangers Download PDF

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Publication number
WO1994029472A3
WO1994029472A3 PCT/US1994/006177 US9406177W WO9429472A3 WO 1994029472 A3 WO1994029472 A3 WO 1994029472A3 US 9406177 W US9406177 W US 9406177W WO 9429472 A3 WO9429472 A3 WO 9429472A3
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WO
WIPO (PCT)
Prior art keywords
recombinant
mengoviruses
mengovirus
vector
expression
Prior art date
Application number
PCT/US1994/006177
Other languages
English (en)
Other versions
WO1994029472A2 (fr
WO1994029472A9 (fr
Inventor
Ralf Altmeyer
Der Werf Sylvie Van
Marc Girard
Ann C Palmenberg
Original Assignee
Pasteur Institut
Ralf Altmeyer
Der Werf Sylvie Van
Marc Girard
Ann C Palmenberg
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pasteur Institut, Ralf Altmeyer, Der Werf Sylvie Van, Marc Girard, Ann C Palmenberg filed Critical Pasteur Institut
Priority to JP7501922A priority Critical patent/JPH08511165A/ja
Priority to EP94923174A priority patent/EP0702724A1/fr
Publication of WO1994029472A2 publication Critical patent/WO1994029472A2/fr
Publication of WO1994029472A9 publication Critical patent/WO1994029472A9/fr
Publication of WO1994029472A3 publication Critical patent/WO1994029472A3/fr

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/033—Fusion polypeptide containing a localisation/targetting motif containing a motif for targeting to the internal surface of the plasma membrane, e.g. containing a myristoylation motif
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/16011—Human Immunodeficiency Virus, HIV
    • C12N2740/16111—Human Immunodeficiency Virus, HIV concerning HIV env
    • C12N2740/16122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/10011—Arenaviridae
    • C12N2760/10022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/20011—Rhabdoviridae
    • C12N2760/20111—Lyssavirus, e.g. rabies virus
    • C12N2760/20122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/32011—Picornaviridae
    • C12N2770/32211—Cardiovirus, e.g. encephalomyocarditis virus
    • C12N2770/32222—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/32011—Picornaviridae
    • C12N2770/32211—Cardiovirus, e.g. encephalomyocarditis virus
    • C12N2770/32241—Use of virus, viral particle or viral elements as a vector
    • C12N2770/32243—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Virology (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Communicable Diseases (AREA)
  • Oncology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Physics & Mathematics (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

Cette invention concerne de mengovirus de recombinaison atténués qui expriment une séquence d'acides aminés hétérologue. Les mengovirus de recombinaison peuvent exprimer des séquences d'acides aminés comprenant des déterminants antigéniques de divers pathogènes viraux et sont utiles comme vaccins vivants contre ces pathogènes chez l'homme et l'animal. Par conséquent cette invention concerne également des vaccins renfermant ces mengovirus de recombinaison ou des protéines de ces derniers, des cellules infectées par les mengovirus de recombinaison, l'acide nucléique dérivé de ces mengovirus de recombinaison et des procédés permettant d'induire une réponse immunitaire. Cette invention concerne plus particulièrement un mengovirus de recombinaison comprenant les acides aminés 299-466 de gp120 de l'isolat MN du VIH-I.
PCT/US1994/006177 1993-06-03 1994-06-03 Mengovirus utilise comme vecteur d'expression de polypeptides etrangers WO1994029472A2 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
JP7501922A JPH08511165A (ja) 1993-06-03 1994-06-03 外来性ポリペプチドの発現のためのベクターとしてのメンゴウイルス
EP94923174A EP0702724A1 (fr) 1993-06-03 1994-06-03 Mengovirus utilise comme vecteur d'expression de polypeptides etrangers

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US9053193A 1993-06-03 1993-06-03
US08/090,531 1993-06-03

Publications (3)

Publication Number Publication Date
WO1994029472A2 WO1994029472A2 (fr) 1994-12-22
WO1994029472A9 WO1994029472A9 (fr) 1995-02-02
WO1994029472A3 true WO1994029472A3 (fr) 1995-02-16

Family

ID=22223195

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1994/006177 WO1994029472A2 (fr) 1993-06-03 1994-06-03 Mengovirus utilise comme vecteur d'expression de polypeptides etrangers

Country Status (4)

Country Link
EP (1) EP0702724A1 (fr)
JP (1) JPH08511165A (fr)
CA (1) CA2164297A1 (fr)
WO (1) WO1994029472A2 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000014263A2 (fr) * 1998-09-03 2000-03-16 Board Of Regents, The University Of Texas System Virus de l'hepatite a (hav) recombinant, variants de hav, vaccins a base de hav et procedes de leur preparation
US20030077251A1 (en) * 2001-05-23 2003-04-24 Nicolas Escriou Replicons derived from positive strand RNA virus genomes useful for the production of heterologous proteins

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0302801A1 (fr) * 1987-08-07 1989-02-08 Institut Pasteur Vaccins dont l'épitope caractéristique est incorporé dans une protéine de picornavirus, notamment de poliovirus

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0302801A1 (fr) * 1987-08-07 1989-02-08 Institut Pasteur Vaccins dont l'épitope caractéristique est incorporé dans une protéine de picornavirus, notamment de poliovirus

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
B. DIETZSCHOLD ET AL.: "Structural and immunologocal characterisation of a linear virus neutralising epitope of the rabies virus glycoprotein", J. VIROL., vol. 64, no. 8, 1990, pages 3804 - 3809 *
C. ROSE ET AL.: "Poliovirus antigen chimeras", TRENDS IN BIOTECHNOLOGY, vol. 9, 1991, pages 415 - 421 *
G. DUKE ET AL.: "Attenuation of Mengovirus through genetic engineering of the 5' noncoding poly C tract", NATURE, vol. 343, 1990, pages 474 - 476 *
J. WHITTON ET AL.: "A string of beads vaccine comprisisg linked minigenes", J. VIROL., vol. 67, no. 1, 1993, pages 348 - 352 *

Also Published As

Publication number Publication date
WO1994029472A2 (fr) 1994-12-22
JPH08511165A (ja) 1996-11-26
CA2164297A1 (fr) 1994-12-22
EP0702724A1 (fr) 1996-03-27

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