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WO1994025604A3 - Steroid 21-hydroxylase fragments and assays using the same - Google Patents

Steroid 21-hydroxylase fragments and assays using the same Download PDF

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Publication number
WO1994025604A3
WO1994025604A3 PCT/GB1994/000855 GB9400855W WO9425604A3 WO 1994025604 A3 WO1994025604 A3 WO 1994025604A3 GB 9400855 W GB9400855 W GB 9400855W WO 9425604 A3 WO9425604 A3 WO 9425604A3
Authority
WO
WIPO (PCT)
Prior art keywords
steroid
hydroxylase
polypeptides
assays
fragments
Prior art date
Application number
PCT/GB1994/000855
Other languages
French (fr)
Other versions
WO1994025604A2 (en
Inventor
Jadwiga Maria Furmaniak-Wehr
Original Assignee
R S R Limited
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by R S R Limited filed Critical R S R Limited
Priority to JP6523997A priority Critical patent/JPH09500008A/en
Priority to AU66610/94A priority patent/AU6661094A/en
Publication of WO1994025604A2 publication Critical patent/WO1994025604A2/en
Publication of WO1994025604A3 publication Critical patent/WO1994025604A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0083—Miscellaneous (1.14.99)
    • C12N9/0087—Steroid 21-monooxygenase (1.14.99.10)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Polypeptides comprising an amino acid sequence of steroid 21-hydroxylase containing an epitopic region for adrenal autoantibody are useful in assay for adrenal autoantibodies or the therapy of adrenal autoimmune disease. The polypeptides may be synthetic or expressed by novel transformants transfected with DNA encoding the polypeptides.
PCT/GB1994/000855 1993-04-24 1994-04-22 Steroid 21-hydroxylase fragments and assays using the same WO1994025604A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
JP6523997A JPH09500008A (en) 1993-04-24 1994-04-22 Steroid 21-hydroxylase fragment and assay method using the same
AU66610/94A AU6661094A (en) 1993-04-24 1994-04-22 Steroid 21-hydroxylase fragments and assays using the same

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB9308544.7 1993-04-24
GB939308544A GB9308544D0 (en) 1993-04-24 1993-04-24 Assay

Publications (2)

Publication Number Publication Date
WO1994025604A2 WO1994025604A2 (en) 1994-11-10
WO1994025604A3 true WO1994025604A3 (en) 1996-05-02

Family

ID=10734450

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB1994/000855 WO1994025604A2 (en) 1993-04-24 1994-04-22 Steroid 21-hydroxylase fragments and assays using the same

Country Status (4)

Country Link
JP (1) JPH09500008A (en)
AU (1) AU6661094A (en)
GB (1) GB9308544D0 (en)
WO (1) WO1994025604A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5723343A (en) * 1995-08-28 1998-03-03 University Of Florida Autoantibodies in patients with acquired hypoparathyroidism and assay method therefor
GB9725105D0 (en) 1997-11-28 1998-01-28 Rsr Ltd Diagnosis of autoimmune adrenal disease

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1988009932A1 (en) * 1987-06-03 1988-12-15 Amrad Corporation Limited NUCLEAR ANTIGEN La
WO1989009222A1 (en) * 1988-03-23 1989-10-05 The Johns Hopkins University Cloned human topoisomerase i
DE3929822A1 (en) * 1989-09-07 1991-03-14 Max Planck Gesellschaft DNA vector including multiple cloning site - for cloning, specific mutagenesis, sequencing and optimised expression of inserted genes, and construction of gene banks
GB2256046A (en) * 1992-07-31 1992-11-25 R S R Limited Assay for adrenal autoantibodies useful in the diagnosis of addison's disease
WO1993005151A1 (en) * 1991-09-12 1993-03-18 The Johns Hopkins University Cloned human cenp-c antigen

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1988009932A1 (en) * 1987-06-03 1988-12-15 Amrad Corporation Limited NUCLEAR ANTIGEN La
WO1989009222A1 (en) * 1988-03-23 1989-10-05 The Johns Hopkins University Cloned human topoisomerase i
DE3929822A1 (en) * 1989-09-07 1991-03-14 Max Planck Gesellschaft DNA vector including multiple cloning site - for cloning, specific mutagenesis, sequencing and optimised expression of inserted genes, and construction of gene banks
WO1993005151A1 (en) * 1991-09-12 1993-03-18 The Johns Hopkins University Cloned human cenp-c antigen
GB2256046A (en) * 1992-07-31 1992-11-25 R S R Limited Assay for adrenal autoantibodies useful in the diagnosis of addison's disease

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
J. BEDNAREK ET AL: "Steroid 21-hydroxylase is a major autoantigen involved in adult onset autoimmune Addison's disease", FEBS LETTERS., vol. 309, no. 1, August 1992 (1992-08-01), AMSTERDAM NL, pages 51 - 55 *
N. WEDLOCK ET AL: "Autoimmune Addison's disease. Analysis of autoantibody binding sites on human steroid 21-hydroxylase", FEBS LETTERS, vol. 332, no. 1,2, October 1993 (1993-10-01), pages 123 - 126 *
P. WHITE ET AL: "Structure of human steroid 21-hydroxylase", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA., vol. 83, July 1986 (1986-07-01), WASHINGTON US, pages 5111 - 5115 *

Also Published As

Publication number Publication date
GB9308544D0 (en) 1993-06-09
JPH09500008A (en) 1997-01-07
WO1994025604A2 (en) 1994-11-10
AU6661094A (en) 1994-11-21

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