WO1993021322A9 - Procede de regulation et de determination de la morphogenese d'organes vegetaux, un gene homeotique, un element promoteur utilise a cet effet, et utilisations connexes - Google Patents
Procede de regulation et de determination de la morphogenese d'organes vegetaux, un gene homeotique, un element promoteur utilise a cet effet, et utilisations connexesInfo
- Publication number
- WO1993021322A9 WO1993021322A9 PCT/US1993/003508 US9303508W WO9321322A9 WO 1993021322 A9 WO1993021322 A9 WO 1993021322A9 US 9303508 W US9303508 W US 9303508W WO 9321322 A9 WO9321322 A9 WO 9321322A9
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- gene
- whorl
- petunia
- flower
- petal
- Prior art date
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Definitions
- the co-suppression of pMADSl was manifested in a gradation of phenotypes ranging from a decrease in petal pigmentation (five out of twenty transgenic plants, SD15 see Figure 20A), reduced petal growth (one out of twenty, SD6 see Figure 20B), reduced growth and differentiation (one of twenty, SD12 see Figure 20C) to a complete lack of petal differentiation, resulting in sepaloid structures in the second whorl (one of twenty, SD3 see Figure 20D).
- a partial petal differentiation of the second floral whorl in SD3 plants could occur upon aging of the plant (mainly after anthesis has occurred), resulting in slightly pigmented sepaloid second whorl structures (SD3, Figure 20E).
Abstract
Procédé de régulation de la morphogénèse d'organes végétaux consistant à provoquer la sous-expression d'un gène homéotique responsable de la détermination de l'identité de l'organe végétal en question. Le procédé s'applique particulièrement aux fleurs et, à titre d'un premier exemple, le pétunia a été modifié pour présenter un verticille supplémentaire de pétales dérivés de la modification des cépales. Le verticille supplémentaire de pétales peut être formé par la conversion des cépales en pétales, et/ou par la stimulation de la croissance des structures de pétales existantes afin de définir des structures inversées qui apparaissent et présentent un second verticille. Le gène homéotique pétale vert chez le pétunia a été cloné et caractérisé et est divulgué dans la présente invention, seul et également en association avec des séquences de régulation de l'expression et dans des vecteurs pour l'expression ectopique transgénique. Dans un second exemple la même fleur a été modifiée afin de présenter un verticille de pétales ayant des caractéristiques cépaloïdes sur au moins une portion des pétales. L'invention concerne aussi la construction d'un promoteur dérivé du promoteur CaMV 35S et désigné dans l'invention m35S. Ce promoteur est particulièrement approprié pour la construction de transgènes homéotiques selon le procédé décrit dans l'invention. Les fleurs pétunias ayant un verticille de pétales modifiés préparées par le présent procédé sont églament décrites.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP93909324A EP0640132A1 (fr) | 1992-04-13 | 1993-04-13 | Procede de regulation et de determination de la morphogenese d'organes vegetaux, un gene homeotique, un element promoteur utilise a cet effet, et utilisations connexes |
| JP5518586A JPH07505779A (ja) | 1992-04-13 | 1993-04-13 | 植物器官の形態形成を制御し決定するための方法，そのためのホメオ遺伝子，プロモーター，及び関連するその用途 |
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US86758092A | 1992-04-13 | 1992-04-13 | |
| US07/867,580 | 1992-04-13 | ||
| US90958992A | 1992-07-06 | 1992-07-06 | |
| US07/909,589 | 1992-07-06 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| WO1993021322A1 WO1993021322A1 (fr) | 1993-10-28 |
| WO1993021322A9 true WO1993021322A9 (fr) | 1994-01-20 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/US1993/003508 WO1993021322A1 (fr) | 1992-04-13 | 1993-04-13 | Procede de regulation et de determination de la morphogenese d'organes vegetaux, un gene homeotique, un element promoteur utilise a cet effet, et utilisations connexes |
Country Status (3)
| Country | Link |
|---|---|
| EP (1) | EP0640132A1 (fr) |
| JP (1) | JPH07505779A (fr) |
| WO (1) | WO1993021322A1 (fr) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1994000582A2 (fr) * | 1992-06-30 | 1994-01-06 | Bruinsma Seeds B.V. | Procede d'obtention d'une plante a morphologie florale modifiee, et procede de protection des plantes contre les insectes nuisibles |
| NZ526378A (en) * | 2000-11-28 | 2007-12-21 | Du Pont | Nucleic acid fragments encoding floral development proteins in plants and seeds |
| US20050066394A1 (en) * | 2000-11-28 | 2005-03-24 | Olga Danilevskaya | Floral development genes |
| CN111088261B (zh) * | 2020-01-15 | 2021-09-28 | 南京林业大学 | 一种矮牵牛花器官发育基因PhDof28及其应用 |
-
1993
- 1993-04-13 EP EP93909324A patent/EP0640132A1/fr not_active Withdrawn
- 1993-04-13 JP JP5518586A patent/JPH07505779A/ja active Pending
- 1993-04-13 WO PCT/US1993/003508 patent/WO1993021322A1/fr not_active Application Discontinuation
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