WO1992013229A3 - Procede d'analyse par electrophorese capillaire avec detection par fluorescence - Google Patents
Procede d'analyse par electrophorese capillaire avec detection par fluorescence Download PDFInfo
- Publication number
- WO1992013229A3 WO1992013229A3 PCT/FR1992/000011 FR9200011W WO9213229A3 WO 1992013229 A3 WO1992013229 A3 WO 1992013229A3 FR 9200011 W FR9200011 W FR 9200011W WO 9213229 A3 WO9213229 A3 WO 9213229A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- capillary
- laser beam
- analysis method
- fluorescence
- fluorescence detection
- Prior art date
Links
- 238000004458 analytical method Methods 0.000 title abstract 2
- 238000005251 capillar electrophoresis Methods 0.000 title abstract 2
- 238000001917 fluorescence detection Methods 0.000 title abstract 2
- 238000001962 electrophoresis Methods 0.000 abstract 1
- 150000002500 ions Chemical class 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
- G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
- G01N33/561—Immunoelectrophoresis
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Pathology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Electrochemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
Procédé d'analyse par électrophorèse capillaire avec détection par fluorescence, dans lequel on fait passer dans un capillaire d'électrophorèse de diamètre intérieur inférieur ou égal à 100 νm un échantillon contenant les produits à analyser marqués au besoin par un agent de fluorescence. On utilise pour exciter la fluorescence un faisceau laser focalisé sur le capillaire par un dispositif de concentration de la lumière et on détecte la fluorescence de manière colinéaire, soit dans l'axe d'illumination par le faisceau laser et du même côté du capillaire, à l'aide du dispositif de concentration de la lumière utilisé pour focaliser le faisceau laser et d'un photodétecteur. Ce procédé permet un grand nombre d'applications, notamment en biologie moléculaire et dans le domaine pharmaceutique, ainsi que pour l'analyse de traces d'ions.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FR9100144A FR2671407B1 (fr) | 1991-01-08 | 1991-01-08 | Procede d'analyse par electrophorese capillaire avec detection par fluorescence, et dispositifs de mise en óoeuvre. |
FR91/00144 | 1991-01-08 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO1992013229A2 WO1992013229A2 (fr) | 1992-08-06 |
WO1992013229A3 true WO1992013229A3 (fr) | 1992-10-15 |
Family
ID=9408509
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/FR1992/000011 WO1992013229A2 (fr) | 1991-01-08 | 1992-01-08 | Procede d'analyse par electrophorese capillaire avec detection par fluorescence |
Country Status (2)
Country | Link |
---|---|
FR (1) | FR2671407B1 (fr) |
WO (1) | WO1992013229A2 (fr) |
Families Citing this family (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5348633A (en) * | 1993-01-22 | 1994-09-20 | Northeastern University | Method for quantitating trace amounts of an analyte in a sample by affinity capillary electrophoresis |
WO1995021378A1 (fr) * | 1994-02-07 | 1995-08-10 | Perkin-Elmer Corporation | Systeme d'electrophorese par fluorescence pour analyse de polynucleotides |
DE4411998A1 (de) * | 1994-04-08 | 1995-10-19 | Bioquant Gmbh Unternehmen Fuer | Kollektor für optische Strahlung |
US5582705A (en) * | 1995-05-19 | 1996-12-10 | Iowa State University Research Foundation, Inc. | Multiplexed capillary electrophoresis system |
DE10140680A1 (de) * | 2001-08-24 | 2003-03-06 | Bayer Ag | Spektroskopisches Testsystem auf Basis von Mikrokapillaren |
US6905583B2 (en) * | 2002-12-13 | 2005-06-14 | Aclara Biosciences, Inc. | Closed-loop control of electrokinetic processes in microfluidic devices based on optical readings |
CN102879365A (zh) * | 2012-09-21 | 2013-01-16 | 常州大学 | 一种毛细管电泳荧光检测装置 |
CN106596626B (zh) * | 2016-11-29 | 2019-04-09 | 武汉大学 | 瞬态荧光测量材料热扩散率的方法及装置 |
CN108593748B (zh) * | 2018-01-26 | 2024-04-30 | 南京溯远基因科技有限公司 | 毛细管及dna测序仪 |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2558262A1 (fr) * | 1984-01-16 | 1985-07-19 | California Inst Of Techn | Procede et systeme pour l'analyse par electrophorese de fragments d'adn obtenus par sequencage |
EP0216600A2 (fr) * | 1985-09-18 | 1987-04-01 | The Board Of Trustees Of The Leland Stanford Junior University | Fluorescence par excitation laser pour la détection de séparation électrocinétique |
EP0442315A1 (fr) * | 1990-01-29 | 1991-08-21 | Waters Investments Limited | Procédé de séparation d'espèces ioniques par électrophorèse capillaire |
-
1991
- 1991-01-08 FR FR9100144A patent/FR2671407B1/fr not_active Expired - Lifetime
-
1992
- 1992-01-08 WO PCT/FR1992/000011 patent/WO1992013229A2/fr active Application Filing
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2558262A1 (fr) * | 1984-01-16 | 1985-07-19 | California Inst Of Techn | Procede et systeme pour l'analyse par electrophorese de fragments d'adn obtenus par sequencage |
EP0216600A2 (fr) * | 1985-09-18 | 1987-04-01 | The Board Of Trustees Of The Leland Stanford Junior University | Fluorescence par excitation laser pour la détection de séparation électrocinétique |
EP0442315A1 (fr) * | 1990-01-29 | 1991-08-21 | Waters Investments Limited | Procédé de séparation d'espèces ioniques par électrophorèse capillaire |
Non-Patent Citations (4)
Title |
---|
ANALYTICAL CHEMISTRY, vol. 53, no. 8, juillet 1981, pages 1298-1302, J.W. JORGENSON et al.: "Zone Electrophoresis in Open-Tubular Glass Capillaries", abrégé * |
ANALYTICAL CHEMISTRY, vol. 62, no. 12, juin 1990, pages 403R-414R, California, US; W.G. KUHR: "Capillary Electrophoresis", page 406R,407R * |
APPLIED SPECTROSCOPY, vol. 44, no. 5, 1990, Tennessee, US, Y.-F. CHENG et al.: "Charge-coupled device fluorescence detection for capillary-zone electrophoresis (CCD-CZE), abrégé * |
JOURNAL OF CHROMATOGRAPHY, vol. 502, 1990, pages 247-255, Amsterdam, NL, L. HERNANDEZ et al.: "Detection and quantification of capillary electrophoresis zones by fluorescence microscopy", abrégé, pages 248,255, figures 1,2 * |
Also Published As
Publication number | Publication date |
---|---|
FR2671407A1 (fr) | 1992-07-10 |
WO1992013229A2 (fr) | 1992-08-06 |
FR2671407B1 (fr) | 1994-08-05 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Albin et al. | Fluorescence detection in capillary electrophoresis: evaluation of derivatizing reagents and techniques | |
Ueda et al. | Separation of naphthalene-2, 3-dicarboxaldehyde-labeled amino acids by high-performance capillary electrophoresis with laser-induced fluorescence detection | |
AU638649B2 (en) | Method of and apparatus for determining the similarity of a biological analyte from a model constructed from known biological fluids | |
AU5656996A (en) | Molecular imaging | |
DE69835142D1 (de) | VORRICHTUNG zur Bestimmung VON STÖRENDEN SUBSTANZEN im PLASMA | |
FI935244A0 (fi) | Bestaemningsanordning | |
EP1650549A3 (fr) | Capteur de plasmons de surface | |
EP2077443A3 (fr) | Appareil de mesure quantitative par chromatographie | |
FI951673A0 (fi) | Menetelmä ja laite glukoosianalyysiä varten biologisessa matriisissa | |
EP1837647A3 (fr) | Système d'électrophorèse capillaire multiplexe | |
EP0244929A3 (en) | Fluorescent polymeric indicator and sensor | |
EP0335725A3 (fr) | Appareil et méthode de détection de fluorescence ou de diffusion de lumière | |
EP1873512A3 (fr) | Appareil et procédé de détection de fluorescent dans des échantillons biologiques | |
NO914826D0 (no) | Immobilisert polymersyntese i svaert stor skala | |
CA2105512A1 (fr) | Detection amelioree de microorganismes dans des echantillons | |
DE69401717D1 (de) | Verfahren zur Aktivierung von polyanionischen,fluoreszierenden Farbstoffen in schwach dielektrischen Medien mit quaternären Oniumverbindungen | |
WO1992013229A3 (fr) | Procede d'analyse par electrophorese capillaire avec detection par fluorescence | |
ES2081991T3 (es) | Deteccion o cuantificacion de varios analitos utilizando tecnicas de marcacion. | |
ATE177434T1 (de) | Antikoagulierende zusammensetzung | |
CA2154136A1 (fr) | Capteur optique pour hemoculture | |
EP0404258A3 (fr) | Tube à essai avec surface extérieure lenticulaire, notament pour les analyses cliniques automatisées | |
ATE107418T1 (de) | Nachweis von analyt in partikel enthaltenden proben. | |
FR2649487B1 (fr) | Capillaire d'electrophorese a dispositif optique incorpore | |
DK0441470T3 (da) | Fremgangsmåde til separation og måling af sporbestanddele | |
ATE157166T1 (de) | Vorrichtung zur messung von optischen eigenschaften durchsichtiger gegenstände |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
AK | Designated states |
Kind code of ref document: A2 Designated state(s): JP US |
|
AL | Designated countries for regional patents |
Kind code of ref document: A2 Designated state(s): AT BE CH DE DK ES FR GB GR IT LU MC NL SE |
|
AK | Designated states |
Kind code of ref document: A3 Designated state(s): JP US |
|
AL | Designated countries for regional patents |
Kind code of ref document: A3 Designated state(s): AT BE CH DE DK ES FR GB GR IT LU MC NL SE |
|
DFPE | Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101) | ||
122 | Ep: pct application non-entry in european phase |