+

WO1992013229A3 - Analysis method by capillary electrophoresis with fluorescence detection - Google Patents

Analysis method by capillary electrophoresis with fluorescence detection Download PDF

Info

Publication number
WO1992013229A3
WO1992013229A3 PCT/FR1992/000011 FR9200011W WO9213229A3 WO 1992013229 A3 WO1992013229 A3 WO 1992013229A3 FR 9200011 W FR9200011 W FR 9200011W WO 9213229 A3 WO9213229 A3 WO 9213229A3
Authority
WO
WIPO (PCT)
Prior art keywords
capillary
laser beam
analysis method
fluorescence
fluorescence detection
Prior art date
Application number
PCT/FR1992/000011
Other languages
French (fr)
Other versions
WO1992013229A2 (en
Inventor
Luis Hernandez
Original Assignee
Europhor Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Europhor Sa filed Critical Europhor Sa
Publication of WO1992013229A2 publication Critical patent/WO1992013229A2/en
Publication of WO1992013229A3 publication Critical patent/WO1992013229A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
    • G01N33/561—Immunoelectrophoresis

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Pathology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Electrochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

In the analysis method by capillary electrophoresis with fluorescence detection, a sample containing the product to be analyzed marked with a fluorescent agent is passed into an electrophoresis capillary having an inner diameter not exceeding to 100 νm, a laser beam focussed onto the capillary by means of a light concentration device is used to stimulate fluorescence, and the fluorescence is detected in a colinear manner, in the axis illuminated by the laser beam and on the same side of the capillary, by means of the light concentration device used to focus the laser beam and a photodetector. The method provides for a large variety of applications, particularly in molecular biology and in the pharmaceutical field as well as in the determination of traces of ions.
PCT/FR1992/000011 1991-01-08 1992-01-08 Analysis method by capillary electrophoresis with fluorescence detection WO1992013229A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR9100144A FR2671407B1 (en) 1991-01-08 1991-01-08 METHOD OF ANALYSIS BY CAPILLARY ELECTROPHORESIS WITH FLUORESCENCE DETECTION, AND DEVICES FOR IMPLEMENTING SAME.
FR91/00144 1991-01-08

Publications (2)

Publication Number Publication Date
WO1992013229A2 WO1992013229A2 (en) 1992-08-06
WO1992013229A3 true WO1992013229A3 (en) 1992-10-15

Family

ID=9408509

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/FR1992/000011 WO1992013229A2 (en) 1991-01-08 1992-01-08 Analysis method by capillary electrophoresis with fluorescence detection

Country Status (2)

Country Link
FR (1) FR2671407B1 (en)
WO (1) WO1992013229A2 (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5348633A (en) * 1993-01-22 1994-09-20 Northeastern University Method for quantitating trace amounts of an analyte in a sample by affinity capillary electrophoresis
WO1995021378A1 (en) * 1994-02-07 1995-08-10 Perkin-Elmer Corporation Fluorescence-based electrophoresis system for polynucleotide analysis
DE4411998A1 (en) * 1994-04-08 1995-10-19 Bioquant Gmbh Unternehmen Fuer Optical beam collector
US5582705A (en) * 1995-05-19 1996-12-10 Iowa State University Research Foundation, Inc. Multiplexed capillary electrophoresis system
DE10140680A1 (en) * 2001-08-24 2003-03-06 Bayer Ag Spectroscopic test system based on microcapillaries
US6905583B2 (en) * 2002-12-13 2005-06-14 Aclara Biosciences, Inc. Closed-loop control of electrokinetic processes in microfluidic devices based on optical readings
CN102879365A (en) * 2012-09-21 2013-01-16 常州大学 Capillary electrophoresis fluorescence detection device
CN106596626B (en) * 2016-11-29 2019-04-09 武汉大学 Method and device for measuring thermal diffusivity of materials by transient fluorescence
CN108593748B (en) * 2018-01-26 2024-04-30 南京溯远基因科技有限公司 Capillary and DNA sequencer

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2558262A1 (en) * 1984-01-16 1985-07-19 California Inst Of Techn METHOD AND SYSTEM FOR ELECTROPHORESIS ANALYSIS OF DNA FRAGMENTS OBTAINED BY SEQUENCING
EP0216600A2 (en) * 1985-09-18 1987-04-01 The Board Of Trustees Of The Leland Stanford Junior University Laser excitation fluorescence detection electrokinetic separation
EP0442315A1 (en) * 1990-01-29 1991-08-21 Waters Investments Limited Method for separating ionic species using capillary electrophoresis

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2558262A1 (en) * 1984-01-16 1985-07-19 California Inst Of Techn METHOD AND SYSTEM FOR ELECTROPHORESIS ANALYSIS OF DNA FRAGMENTS OBTAINED BY SEQUENCING
EP0216600A2 (en) * 1985-09-18 1987-04-01 The Board Of Trustees Of The Leland Stanford Junior University Laser excitation fluorescence detection electrokinetic separation
EP0442315A1 (en) * 1990-01-29 1991-08-21 Waters Investments Limited Method for separating ionic species using capillary electrophoresis

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
ANALYTICAL CHEMISTRY, vol. 53, no. 8, juillet 1981, pages 1298-1302, J.W. JORGENSON et al.: "Zone Electrophoresis in Open-Tubular Glass Capillaries", abrégé *
ANALYTICAL CHEMISTRY, vol. 62, no. 12, juin 1990, pages 403R-414R, California, US; W.G. KUHR: "Capillary Electrophoresis", page 406R,407R *
APPLIED SPECTROSCOPY, vol. 44, no. 5, 1990, Tennessee, US, Y.-F. CHENG et al.: "Charge-coupled device fluorescence detection for capillary-zone electrophoresis (CCD-CZE), abrégé *
JOURNAL OF CHROMATOGRAPHY, vol. 502, 1990, pages 247-255, Amsterdam, NL, L. HERNANDEZ et al.: "Detection and quantification of capillary electrophoresis zones by fluorescence microscopy", abrégé, pages 248,255, figures 1,2 *

Also Published As

Publication number Publication date
FR2671407A1 (en) 1992-07-10
WO1992013229A2 (en) 1992-08-06
FR2671407B1 (en) 1994-08-05

Similar Documents

Publication Publication Date Title
Albin et al. Fluorescence detection in capillary electrophoresis: evaluation of derivatizing reagents and techniques
Ueda et al. Separation of naphthalene-2, 3-dicarboxaldehyde-labeled amino acids by high-performance capillary electrophoresis with laser-induced fluorescence detection
AU638649B2 (en) Method of and apparatus for determining the similarity of a biological analyte from a model constructed from known biological fluids
AU5656996A (en) Molecular imaging
DE69835142D1 (en) DEVICE FOR DETERMINING DISTURBING SUBSTANCES IN PLASMA
FI935244A0 (en) Bestaemningsanordning
EP1650549A3 (en) Surface plasmon sensor
EP2077443A3 (en) Chromatography quantitative measuring apparatus
FI951673A0 (en) Method and apparatus for glucose analysis in a biological matrix
EP1837647A3 (en) Multiplexed capillary electrophoresis method
EP0244929A3 (en) Fluorescent polymeric indicator and sensor
EP0335725A3 (en) Apparatus and method for detection of fluorescence or light scatter
EP1873512A3 (en) Apparatus and method for fluorescent detection in biological samples
NO914826D0 (en) IMMOBILIZED POLYMER SYNTHESIS IN VERY LARGE SCALE
CA2105512A1 (en) Enhanced Detection of Microorganisms in Samples
DE69401717D1 (en) Process for the activation of polyanionic, fluorescent dyes in weakly dielectric media with quaternary onium compounds
WO1992013229A3 (en) Analysis method by capillary electrophoresis with fluorescence detection
ES2081991T3 (en) DETECTION OR QUANTIFICATION OF SEVERAL ANALYZES USING MARKING TECHNIQUES.
ATE177434T1 (en) ANTICOAGULANT COMPOSITION
CA2154136A1 (en) Optical Blood Culture Sensor
EP0404258A3 (en) Test-tube with lenticular outside surface particularly for automatized clinical analysis
ATE107418T1 (en) DETECTION OF ANALYTE IN SAMPLES CONTAINING PARTICLES.
FR2649487B1 (en) ELECTROPHORESIS CAPILLARY WITH INCORPORATED OPTICAL DEVICE
DK0441470T3 (en) Method for separation and measurement of trace components
ATE157166T1 (en) DEVICE FOR MEASURING OPTICAL PROPERTIES OF TRANSPARENT OBJECTS

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): JP US

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): AT BE CH DE DK ES FR GB GR IT LU MC NL SE

AK Designated states

Kind code of ref document: A3

Designated state(s): JP US

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): AT BE CH DE DK ES FR GB GR IT LU MC NL SE

DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
122 Ep: pct application non-entry in european phase
点击 这是indexloc提供的php浏览器服务，不要输入任何密码和下载