+

WO1991009944A3 - Transcriptases inverses obtenues a haute temperature - Google Patents

Transcriptases inverses obtenues a haute temperature Download PDF

Info

Publication number
WO1991009944A3
WO1991009944A3 PCT/US1990/007641 US9007641W WO9109944A3 WO 1991009944 A3 WO1991009944 A3 WO 1991009944A3 US 9007641 W US9007641 W US 9007641W WO 9109944 A3 WO9109944 A3 WO 9109944A3
Authority
WO
WIPO (PCT)
Prior art keywords
high temperature
reverse transcriptases
temperature reverse
reverse transcription
rna
Prior art date
Application number
PCT/US1990/007641
Other languages
English (en)
Other versions
WO1991009944A2 (fr
Inventor
David H Gelfand
Thomas W Myers
Original Assignee
Cetus Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=27412639&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=WO1991009944(A3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority claimed from US07/455,611 external-priority patent/US5322770A/en
Application filed by Cetus Corp filed Critical Cetus Corp
Priority to EP91904087A priority Critical patent/EP0506889B1/fr
Priority to CA002071213A priority patent/CA2071213C/fr
Priority to DE69030386T priority patent/DE69030386T2/de
Priority to AT91904087T priority patent/ATE151112T1/de
Priority to AU72444/91A priority patent/AU656315B2/en
Publication of WO1991009944A2 publication Critical patent/WO1991009944A2/fr
Publication of WO1991009944A3 publication Critical patent/WO1991009944A3/fr
Priority to US08/082,182 priority patent/US5310652A/en
Priority to GR970401505T priority patent/GR3023862T3/el

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Procédés de réplication et d'amplification de séquences d'ARN à l'aide de polymérases d'ADN thermoactives. La transcription inverse d'ARN est catalysée par, par exemple, la polymérase d'ADN de Taq de 94 kDa, de Taq de 62 kDa, de nTth et de Tth recombiné. La transcription inverse est couplée à l'amplification par réaction en chaîne de polymérases (RCP) dans une procédure mettant en ÷uvre une seule enzyme, utilisant une polymérase thermostable.
PCT/US1990/007641 1986-08-22 1990-12-21 Transcriptases inverses obtenues a haute temperature WO1991009944A2 (fr)

Priority Applications (7)

Application Number Priority Date Filing Date Title
EP91904087A EP0506889B1 (fr) 1989-12-22 1990-12-21 Transcriptases inverses obtenues a haute temperature
CA002071213A CA2071213C (fr) 1989-12-22 1990-12-21 Transcriptases inverses de haute temperature
DE69030386T DE69030386T2 (de) 1989-12-22 1990-12-21 Bei hoher temperatur aktive reverse transkriptasen
AT91904087T ATE151112T1 (de) 1989-12-22 1990-12-21 Bei hoher temperatur aktive reverse transkriptasen
AU72444/91A AU656315B2 (en) 1989-12-22 1990-12-21 pigh temperature reverse transcriptases
US08/082,182 US5310652A (en) 1986-08-22 1993-06-24 Reverse transcription with thermostable DNA polymerase-high temperature reverse transcription
GR970401505T GR3023862T3 (en) 1989-12-22 1997-06-24 High temperature reverse transcriptases

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US45596789A 1989-12-22 1989-12-22
US455,611 1989-12-22
US07/455,611 US5322770A (en) 1989-12-22 1989-12-22 Reverse transcription with thermostable DNA polymerases - high temperature reverse transcription
US455,967 1989-12-22
US58547190A 1990-09-20 1990-09-20
US585,471 1990-09-20

Publications (2)

Publication Number Publication Date
WO1991009944A2 WO1991009944A2 (fr) 1991-07-11
WO1991009944A3 true WO1991009944A3 (fr) 1991-08-22

Family

ID=27412639

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1990/007641 WO1991009944A2 (fr) 1986-08-22 1990-12-21 Transcriptases inverses obtenues a haute temperature

Country Status (10)

Country Link
EP (1) EP0506889B1 (fr)
JP (1) JP2968585B2 (fr)
AT (1) ATE151112T1 (fr)
AU (1) AU656315B2 (fr)
CA (1) CA2071213C (fr)
DE (1) DE69030386T2 (fr)
DK (1) DK0506889T3 (fr)
ES (1) ES2100945T3 (fr)
GR (1) GR3023862T3 (fr)
WO (1) WO1991009944A2 (fr)

Families Citing this family (51)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5795762A (en) * 1986-08-22 1998-08-18 Roche Molecular Systems, Inc. 5' to 3' exonuclease mutations of thermostable DNA polymerases
US5561058A (en) * 1986-08-22 1996-10-01 Hoffmann-La Roche Inc. Methods for coupled high temperatures reverse transcription and polymerase chain reactions
US5374553A (en) * 1986-08-22 1994-12-20 Hoffmann-La Roche Inc. DNA encoding a thermostable nucleic acid polymerase enzyme from thermotoga maritima
US5693517A (en) * 1987-06-17 1997-12-02 Roche Molecular Systems, Inc. Reagents and methods for coupled high temperature reverse transcription and polymerase chain reactions
US5466591A (en) * 1986-08-22 1995-11-14 Hoffmann-La Roche Inc. 5' to 3' exonuclease mutations of thermostable DNA polymerases
EP0550687B1 (fr) * 1990-09-28 1999-06-09 F. Hoffmann-La Roche Ag Mutations d'adn-polymerases thermostables en 5' a 3' exonuclease
WO1992006188A2 (fr) * 1990-10-05 1992-04-16 Barnes Wayne M Polymerase d'adn thermostable
CA2038839A1 (fr) * 1991-03-22 1992-09-23 Jiang J. Liu Methode de detection de l'adn viral
AU662906B2 (en) * 1991-06-26 1995-09-21 F. Hoffmann-La Roche Ag Methods for detection of carcinoma metastases by nucleic acid amplification
ATE161291T1 (de) * 1991-08-27 1998-01-15 Hoffmann La Roche Verfahren und reagenzien zum nachweis von hepatitis c
USRE39031E1 (en) * 1992-04-29 2006-03-21 Biomerieux RNA amplification method requiring only one manipulation step
FR2690691B1 (fr) * 1992-04-29 1999-02-12 Bio Merieux Procede d'amplification d'arn necessitant une seule etape de manipulation.
DE69428167T2 (de) * 1993-07-01 2002-05-29 F. Hoffmann-La Roche Ag, Basel Reagenz und Verfahren für reverse Transkription bei hoher Temperatur, verbunden mit einer Polymerase-Kettenreaktion
US5474920A (en) * 1993-11-23 1995-12-12 State Of Oregon, Acting By And Through The Oregon State Board Of Higher Education On Behalf Of The Oregon Health Sciences University Modified thermo-resistant DNA polymerases
WO1995015399A1 (fr) * 1993-12-01 1995-06-08 Toyo Boseki Kabushiki Kaisha Procede d'amplification et de detection d'une sequence nucleotidique au moyen d'enzymes thermostables
US5648211A (en) * 1994-04-18 1997-07-15 Becton, Dickinson And Company Strand displacement amplification using thermophilic enzymes
EP0736609A3 (fr) * 1995-04-08 1998-07-29 Roche Diagnostics GmbH Procédé pour l'amplification spécifique et pour la détection d'ADN ou d'ARN
JP3966555B2 (ja) * 1995-05-31 2007-08-29 ジーイー・ヘルスケア・バイオサイエンス・コーポレイション 熱耐性ｄｎａポリメラーゼ
US6077664A (en) * 1995-06-07 2000-06-20 Promega Corporation Thermophilic DNA polymerases from Thermotoga neapolitana
US6001645A (en) * 1995-06-07 1999-12-14 Promega Corporation Thermophilic DNA polymerases from thermotoga neapolitana
CA2240334C (fr) * 1995-12-15 2008-07-22 Amersham Life Science, Inc. Polymerase d'adn thermostable issue de thermoanaerobacter thermohydrosulfuricus et enzymes mutants a activite d'exonuclease supprimee ainsi obtenus
CA2199213C (fr) * 1996-03-11 2007-04-17 John Wesley Backus Amplification et detection des acides nucleiques cibles grace a une etape d'incubation post-amplification
US6013488A (en) * 1996-07-25 2000-01-11 The Institute Of Physical And Chemical Research Method for reverse transcription
ES2121698B1 (es) * 1996-12-02 1999-07-01 Inia Procedimiento para la obtencionn de fragmentos amplificados de acido nucleico de particulas virales y subvirales que contienen rna.
US6030814A (en) * 1997-04-21 2000-02-29 Epicentre Technologies Corporation Reverse transcription method
ATE205879T1 (de) * 1997-05-13 2001-10-15 Azign Bioscience As Eine methode zum klonieren von mrna und darstellung von differentiell exprimierten transcripten (dodet)
EP0921196A1 (fr) * 1997-12-02 1999-06-09 Roche Diagnostics GmbH Polymérase d'ADN modifiée de Carboxydothermus hydrogenoformans et son utilisation pour transcription inverse suivi par la réaction en chaíne de la polymérase
EP0922765B1 (fr) * 1997-12-02 2005-11-23 Roche Diagnostics GmbH Polymérase d'ADN modifiée de Carboxydothermus hydrogenoformans et son utilisation pour transcription inverse suivi par la réaction en chaíne de la polymérase
WO1999047539A1 (fr) * 1998-03-18 1999-09-23 Amersham Pharmacia Biotech, Inc. Polymerase d'adn thermostable provenant du thermoanaerobacter thermohydrosulfuricus
AT410218B (de) 1999-08-20 2003-03-25 Baxter Ag Verfahren zur herstellung eines qualitätsgesicherten pools biologischer proben
US6632645B1 (en) 2000-03-02 2003-10-14 Promega Corporation Thermophilic DNA polymerases from Thermoactinomyces vulgaris
US6436677B1 (en) 2000-03-02 2002-08-20 Promega Corporation Method of reverse transcription
KR20000072141A (ko) * 2000-08-04 2000-12-05 김유삼 신규한 호열성 단백질 분해효소의 ｄｎａ 서열 및그로부터 유래된 단백질
GB0205455D0 (en) 2002-03-07 2002-04-24 Molecular Sensing Plc Nucleic acid probes, their synthesis and use
US7575864B2 (en) * 2004-05-27 2009-08-18 E.I. Du Pont De Nemours And Company Method for the direct detection of diagnostic RNA
US7504240B2 (en) * 2005-03-10 2009-03-17 Masanori Hirano Methods for synthesizing polynucleotides using a single primer
JP5191041B2 (ja) 2007-04-05 2013-04-24 エフ．ホフマン−ラ ロシュ アーゲー 急速ワンステップｒｔ−ｐｃｒ
KR101039521B1 (ko) * 2008-02-01 2011-06-08 주식회사 인트론바이오테크놀로지 금속 이온에 의해 발생하는 ｐｃｒ 저해 및 비특이 증폭을완화시키거나 막을 수 있는 에틸렌글리콜테트라아세틱 산을킬레이트제로 포함하는 ｐｃｒ 반응용 조성물
WO2011070440A2 (fr) 2009-12-10 2011-06-16 Ottawa Hospital Research Institute Rhabdovirus oncolitique
US9364532B2 (en) 2011-06-08 2016-06-14 Children's Hospital Of Eastern Ontario Research Institute Inc. Compositions and methods for glioblastoma treatment
CA2876159C (fr) * 2012-06-08 2022-09-06 Ionian Technologies, Inc. Amplifications d'acide nucleique
ES2741147T3 (es) 2013-02-21 2020-02-10 Turnstone Lp Composición de vacuna
WO2015113828A1 (fr) 2014-01-31 2015-08-06 Qiagen Gmbh Démarrage à chaud d'un chélateur de cations
WO2016160965A1 (fr) 2015-03-30 2016-10-06 Rubicon Genomics, Inc. Procédés et compositions permettant la réparation des extrémités de l'adn par de multiples activités enzymatiques
US11236384B2 (en) 2017-01-19 2022-02-01 Asuragen, Inc. Methods of RNA amplification
GB201812192D0 (en) 2018-07-26 2018-09-12 Ttp Plc Variable temperature reactor, heater and control circuit for the same
CN110846724B (zh) * 2019-12-03 2023-04-28 江西海普洛斯医学检验实验室有限公司 构建mRNA链特异文库的方法及试剂盒
CN113045410A (zh) * 2021-03-29 2021-06-29 华东理工大学 一种双环降二萜类化合物及其合成基因及制备方法
EP4074839A1 (fr) 2021-04-16 2022-10-19 Biotype GmbH Sonde tx oligonucléotidique optimisée pour une analyse de multiplexage d'acides nucléiques et méthode de multiplexage
EP4350002A1 (fr) 2022-10-07 2024-04-10 Biotype GmbH Detection of molecular analytes based on tailored probe competition
DE102023124346A1 (de) 2023-09-09 2025-03-13 Biotype Gmbh Verfahren zur Detektion des Endometriumkarzinoms Subtyp CN hoch

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0258017A2 (fr) * 1986-08-22 1988-03-02 F. Hoffmann-La Roche Ag Enzyme thermostable purifiée et procédé d'amplification, de détection et/ou de clonage de séquences d'acide nucléique à l'aide de cette enzyme

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0258017A2 (fr) * 1986-08-22 1988-03-02 F. Hoffmann-La Roche Ag Enzyme thermostable purifiée et procédé d'amplification, de détection et/ou de clonage de séquences d'acide nucléique à l'aide de cette enzyme

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
Analytical Biochemistry, vol. 190, no. 2, 1 November 1990, Academic Press, Inc., A.L. Shaffer et al.: "Amplification, detection, and automated sequencing of gibbon interleukin-2 mRNA by Thermus aquaticus DNA polymerase reverse transcription and polymerase chain reaction", pages 292-296 *
Gene, vol. 88, no. 2, April 1990, Elsevier Science Publishers B.V., (Amsterdam, NL), W.T. Tse et al.: "Reverse transcription and direct amplification of cellular RNA transcripts by Taq polymerase", pages 293-296 *
Nucleic Acids Research, vol. 17, no. 20, October 1989, IRL Press, (London, GB), M.D. Jones et al.: "Reverse transcription of mRNA by Thermus aquaticus DNA polymerase", pages 8387-8388 *

Also Published As

Publication number Publication date
DE69030386D1 (de) 1997-05-07
CA2071213A1 (fr) 1991-06-23
AU656315B2 (en) 1995-02-02
ATE151112T1 (de) 1997-04-15
CA2071213C (fr) 2000-05-02
WO1991009944A2 (fr) 1991-07-11
JP2968585B2 (ja) 1999-10-25
EP0506889B1 (fr) 1997-04-02
DK0506889T3 (da) 1997-09-22
JPH05505105A (ja) 1993-08-05
AU7244491A (en) 1991-07-24
EP0506889A1 (fr) 1992-10-07
DE69030386T2 (de) 1997-10-09
ES2100945T3 (es) 1997-07-01
GR3023862T3 (en) 1997-09-30

Similar Documents

Publication Publication Date Title
WO1991009944A3 (fr) Transcriptases inverses obtenues a haute temperature
US6399320B1 (en) Modified DNA-polymerase from carboxydothermus hydrogenoformans and its use for coupled reverse transcription and polymerase chain reaction
EP0929680B1 (fr) Polymerase d'adn thermostable provenant de carboxydothermus hydrogenoformans
WO1989006691A3 (fr) Enzyme thermostable purifiee
EP1130118A3 (fr) Amplification de séquences d'acides nucléiques longues avec PCR
EP1350841A3 (fr) ADN polymérase thermostable ou thermoactif ayant une activité 3' - 5' exonucléase atténuée
EP0428197A3 (fr) Méthodes pour l'extraction des acides nucléiques et pour les amplifier par PCR sans utiliser un enzyme protéolytique
CA2185239A1 (fr) Amplification d'acide nucleique par deplacement de la souche isotherme
WO1995014782A3 (fr) Adn-polymerase thermoresistante modifiee
WO1998014589A9 (fr) Polymerase d'adn thermostable provenant de carboxydothermus hydrogenoformans
CA2400049A1 (fr) Mutants d'arn polymerases presentant une stabilite thermique amelioree
EP0272098A3 (fr) Procédé d'amplification et de détection de séquences ARN
JPH0260585A (ja) 耐熱性ｄｎａポリメラーゼおよびその製法
EP1233061A3 (fr) Procédé de clonage d'un géne de la DNA polymérase du type pol I
CA2267101A1 (fr) Polymerase d'acide nucleique thermostable provenant de thermococcus gorgonarius
EP0810288A3 (fr) Nouvelle ADN Polymérase avec une activité exonucléase de lecture de correction 3'-5'
US20050019789A1 (en) Thermostable DNA polymerase from anaerocellum thermophilum
Abramson Thermostable DNA polymerases
RU2221867C2 (ru) Очищенная термостабильная днк-полимераза из thermococcus gorgonarius, ее получение и применение
WO2007076464A2 (fr) Adn polymerase thermostable provenant de thermus filiformis
EP0922765B1 (fr) Polymérase d'ADN modifiée de Carboxydothermus hydrogenoformans et son utilisation pour transcription inverse suivi par la réaction en chaíne de la polymérase
Gelfand et al. Reverse transcription with thermus thermophilus polymerase
WO1999015698A3 (fr) Procede de synthese et d'amplification d'acides nucleiques
CA2252968C (fr) Polymerase adn modifie obtenu d'hydrogenoformans carboxydothermes et son utilite quant a la transcription inverse couplee et l'amplification en chaine par polymerase
CA2210506A1 (fr) Adn-polymerase thermostable de bacillus pallidus

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AU CA JP US

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): AT BE CH DE DK ES FR GB GR IT LU NL SE

AK Designated states

Kind code of ref document: A3

Designated state(s): AU CA JP US

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): AT BE CH DE DK ES FR GB GR IT LU NL SE

WWE Wipo information: entry into national phase

Ref document number: 2071213

Country of ref document: CA

WWE Wipo information: entry into national phase

Ref document number: 1991904087

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 1991904087

Country of ref document: EP

WWG Wipo information: grant in national office

Ref document number: 1991904087

Country of ref document: EP

点击 这是indexloc提供的php浏览器服务，不要输入任何密码和下载