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WO1989006691A3 - Enzyme thermostable purifiee - Google Patents

Enzyme thermostable purifiee Download PDF

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Publication number
WO1989006691A3
WO1989006691A3 PCT/US1989/000127 US8900127W WO8906691A3 WO 1989006691 A3 WO1989006691 A3 WO 1989006691A3 US 8900127 W US8900127 W US 8900127W WO 8906691 A3 WO8906691 A3 WO 8906691A3
Authority
WO
WIPO (PCT)
Prior art keywords
enzyme
recombinant
thermostable
thermostable enzyme
purified thermostable
Prior art date
Application number
PCT/US1989/000127
Other languages
English (en)
Other versions
WO1989006691A2 (fr
Inventor
David H Gelfand
Susanne Stoffel
Frances C Lawyer
Randall K Saiki
Original Assignee
Cetus Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=22504093&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=WO1989006691(A3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Cetus Corp filed Critical Cetus Corp
Priority to DE68926038T priority Critical patent/DE68926038T3/de
Priority to DE1989902422 priority patent/DE395736T1/de
Priority to EP89902422A priority patent/EP0395736B2/fr
Publication of WO1989006691A2 publication Critical patent/WO1989006691A2/fr
Publication of WO1989006691A3 publication Critical patent/WO1989006691A3/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Detergent Compositions (AREA)

Abstract

On peut utiliser des séquences d'ADN recombinant en codant une ploymérase d'ADN thermostable issue de Thermus aquaticus pour produire une protéine recombinante d'un poids moléculaire d'environ 86 000-95 000 daltons. L'enzyme recombinante thermostable s'utilise dans une réaction en chaîne de cycles de température, dans laquelle au moins une séquence d'acide nucléique est amplifiée en quantité à partir d'une séquence existante à l'aide d'amorces et de triphosphates de nucléotides choisis. L'enzyme est de préférence stockée dans un tampon qui renferme des détergents non ioniques et lui confère une stabilité.
PCT/US1989/000127 1988-01-12 1989-01-12 Enzyme thermostable purifiee WO1989006691A2 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
DE68926038T DE68926038T3 (de) 1988-01-12 1989-01-12 Gereinigtes thermostabiles enzym
DE1989902422 DE395736T1 (de) 1988-01-12 1989-01-12 Gereinigte thermostabile enzyme.
EP89902422A EP0395736B2 (fr) 1988-01-12 1989-01-12 Enzyme thermostable purifiee

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US14344188A 1988-01-12 1988-01-12
US143,441 1988-01-12

Publications (2)

Publication Number Publication Date
WO1989006691A2 WO1989006691A2 (fr) 1989-07-27
WO1989006691A3 true WO1989006691A3 (fr) 1989-11-16

Family

ID=22504093

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1989/000127 WO1989006691A2 (fr) 1988-01-12 1989-01-12 Enzyme thermostable purifiee

Country Status (12)

Country Link
EP (1) EP0395736B2 (fr)
JP (1) JP2511548B2 (fr)
AT (1) ATE135741T1 (fr)
AU (1) AU3062989A (fr)
CA (1) CA1341143C (fr)
DE (1) DE68926038T3 (fr)
HK (1) HK166096A (fr)
IE (1) IE72180B1 (fr)
IL (1) IL88923A (fr)
SG (1) SG46657A1 (fr)
WO (1) WO1989006691A2 (fr)
ZA (1) ZA89262B (fr)

Families Citing this family (48)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5405774A (en) * 1986-08-22 1995-04-11 Hoffmann-La Roche Inc. DNA encoding a mutated thermostable nucleic acid polymerase enzyme from thermus species sps17
US5455170A (en) * 1986-08-22 1995-10-03 Hoffmann-La Roche Inc. Mutated thermostable nucleic acid polymerase enzyme from Thermus species Z05
US6127155A (en) * 1986-08-22 2000-10-03 Roche Molecular Systems, Inc. Stabilized thermostable nucleic acid polymerase compositions containing non-ionic polymeric detergents
US5374553A (en) * 1986-08-22 1994-12-20 Hoffmann-La Roche Inc. DNA encoding a thermostable nucleic acid polymerase enzyme from thermotoga maritima
US5079352A (en) * 1986-08-22 1992-01-07 Cetus Corporation Purified thermostable enzyme
US5618711A (en) * 1986-08-22 1997-04-08 Hoffmann-La Roche Inc. Recombinant expression vectors and purification methods for Thermus thermophilus DNA polymerase
AU627809B2 (en) * 1988-12-16 1992-09-03 Abbott Laboratories Isolating thermostable enzymes from engineered mesophiles
US5270179A (en) * 1989-08-10 1993-12-14 Life Technologies, Inc. Cloning and expression of T5 DNA polymerase reduced in 3'- to-5' exonuclease activity
US5541099A (en) * 1989-08-10 1996-07-30 Life Technologies, Inc. Cloning and expression of T5 DNA polymerase reduced in 3'-to-5' exonuclease activity
US5047342A (en) * 1989-08-10 1991-09-10 Life Technologies, Inc. Cloning and expression of T5 DNA polymerase
ES2121777T3 (es) * 1989-12-22 1998-12-16 Hoffmann La Roche Vectores de expresion recombinantes y metodos de purificacion de la dna polimerasa de thermus thermophilus.
ATE197718T1 (de) * 1990-09-28 2000-12-15 Hoffmann La Roche Gereinigte thermostabile nukleinsäurepolymerase aus thermosipho africanus
EP0550687B1 (fr) * 1990-09-28 1999-06-09 F. Hoffmann-La Roche Ag Mutations d'adn-polymerases thermostables en 5' a 3' exonuclease
WO1992006188A2 (fr) * 1990-10-05 1992-04-16 Barnes Wayne M Polymerase d'adn thermostable
US6083686A (en) * 1990-10-26 2000-07-04 Johnson & Johnson Clinical Diagnostic Systems, Inc. Increased production of Thermus aquaticus DNA polymerase in E. coli
EP1233061A3 (fr) * 1991-06-03 2002-09-04 Takara Shuzo Co., Ltd. Procédé de clonage d'un géne de la DNA polymérase du type pol I
US5338671A (en) * 1992-10-07 1994-08-16 Eastman Kodak Company DNA amplification with thermostable DNA polymerase and polymerase inhibiting antibody
US6410277B1 (en) 1993-02-19 2002-06-25 Takara Shuzo Co., Ltd. DNA polymersases with enhanced length of primer extension
US7465539B1 (en) 1993-02-19 2008-12-16 Takara Bio, Inc. DNA polymerases with enhanced length of primer extension
US5436149A (en) * 1993-02-19 1995-07-25 Barnes; Wayne M. Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension
US5474920A (en) * 1993-11-23 1995-12-12 State Of Oregon, Acting By And Through The Oregon State Board Of Higher Education On Behalf Of The Oregon Health Sciences University Modified thermo-resistant DNA polymerases
US7312052B1 (en) 1993-12-08 2007-12-25 Stratagene California Polymerase compositions and uses thereof
US5622822A (en) 1994-09-13 1997-04-22 Johnson & Johnson Clinical Diagnostics, Inc. Methods for capture and selective release of nucleic acids using polyethyleneimine and an anionic phosphate ester surfactant and amplification of same
US5705366A (en) 1994-09-15 1998-01-06 Johnson & Johnson Clinical Diagnostics, Inc. Coamplification of target nucleic acids using volume exclusion agent in reaction composition, test kit and test device useful therefor
US5582988A (en) 1994-09-15 1996-12-10 Johnson & Johnson Clinical Diagnostics, Inc. Methods for capture and selective release of nucleic acids using weakly basic polymer and amplification of same
US6001645A (en) * 1995-06-07 1999-12-14 Promega Corporation Thermophilic DNA polymerases from thermotoga neapolitana
US6077664A (en) * 1995-06-07 2000-06-20 Promega Corporation Thermophilic DNA polymerases from Thermotoga neapolitana
JP2000516468A (ja) 1996-08-14 2000-12-12 ライフ テクノロジーズ，インコーポレイテッド 核酸増幅および配列決定のための安定な組成物
WO1998042860A1 (fr) 1997-03-21 1998-10-01 Stratagene Extraits de facteur stimulant la polymerase (pef), complexes de proteines pef, proteine pef isolee, et procedes de purification et d'identification
ATE278035T1 (de) 1999-05-04 2004-10-15 Ortho Clinical Diagnostics Inc Schnelle und wirksame dns-immobilisierung aus einer probe ohne die verwendung von zelllysereagenzien
US6436677B1 (en) 2000-03-02 2002-08-20 Promega Corporation Method of reverse transcription
US6632645B1 (en) 2000-03-02 2003-10-14 Promega Corporation Thermophilic DNA polymerases from Thermoactinomyces vulgaris
US7262006B1 (en) 2000-05-01 2007-08-28 Ortho-Clinical Diagnostics, Inc. Rapid and efficient capture of DNA from sample without using cell lysing reagent
US6617136B2 (en) 2001-04-24 2003-09-09 3M Innovative Properties Company Biological sample processing methods and compositions that include surfactants
US20030134292A1 (en) * 2001-10-30 2003-07-17 Farchaus Joseph W. Thermostable DNA polymerases and methods of making same
JP4568571B2 (ja) * 2004-10-06 2010-10-27 ユニバーサル・バイオ・リサーチ株式会社 熱安定性又は熱活性ｄｎａポリメラーゼ及びそれをコードするｄｎａ
JP4699008B2 (ja) * 2004-11-02 2011-06-08 旭化成株式会社 酵素反応試薬
US7972828B2 (en) 2006-12-19 2011-07-05 Sigma-Aldrich Co. Stabilized compositions of thermostable DNA polymerase and anionic or zwitterionic detergent
EP2113574A1 (fr) 2008-04-28 2009-11-04 Biotype AG Substances et procédés pour un dosage de profilage à base d'ADN
US20110250598A1 (en) * 2010-04-12 2011-10-13 Ulrike Fischer Detergent free polymerases
JP5933544B2 (ja) 2010-07-26 2016-06-08 バイオマトリカ， インコーポレーテッド 外界温度で出荷および貯蔵中の血中ｄｎａ、ｒｎａおよびタンパク質ならびに他の生体試料を安定化させるための組成物
WO2012018639A2 (fr) 2010-07-26 2012-02-09 Biomatrica, Inc. Compositions de stabilisation d'adn, d'arn, de protéines salivaires et d'autres échantillons biologiques lors du transport et du stockage à températures ambiantes
WO2012146260A1 (fr) 2011-04-23 2012-11-01 Biolytix Ag Préparation et utilisation de protéines en biologie moléculaire
CN107190078A (zh) 2011-05-06 2017-09-22 凯杰有限公司 包含内部标记引物的核酸的测序、扩增和检测方法
EP2934572A4 (fr) 2012-12-20 2016-11-23 Biomatrica Inc Formulations et procédés pour stabiliser des réactifs pcr
EP3656215B1 (fr) 2014-06-10 2021-08-04 Biomatrica, INC. Stabilisation de thrombocytes à des températures ambiantes
EP4242628A3 (fr) 2015-12-08 2023-11-08 Biomatrica, INC. Réduction de vitesse de sédimentation des érythrocytes
CN110218777B (zh) * 2019-06-14 2023-05-16 苏州天合优才生物科技有限公司 Pcr预混液

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0237362A1 (fr) * 1986-03-13 1987-09-16 F. Hoffmann-La Roche Ag Procédé et nécessaire pour la détection des variations de nucléotides spécifiques et de polymorphismes génétiques dans les acides nucléiques
EP0258017A2 (fr) * 1986-08-22 1988-03-02 F. Hoffmann-La Roche Ag Enzyme thermostable purifiée et procédé d'amplification, de détection et/ou de clonage de séquences d'acide nucléique à l'aide de cette enzyme

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0822261B2 (ja) * 1986-03-07 1996-03-06 株式会社日立製作所 電気掃除機

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0237362A1 (fr) * 1986-03-13 1987-09-16 F. Hoffmann-La Roche Ag Procédé et nécessaire pour la détection des variations de nucléotides spécifiques et de polymorphismes génétiques dans les acides nucléiques
EP0258017A2 (fr) * 1986-08-22 1988-03-02 F. Hoffmann-La Roche Ag Enzyme thermostable purifiée et procédé d'amplification, de détection et/ou de clonage de séquences d'acide nucléique à l'aide de cette enzyme

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
Abstracts of the Annual Meeting of the American Society for Microbiology, Vol. 75, 1975 (US) D. EDGAR et al.: "Purification and Characterization of a DNA Polymerase from an Extreme Thermophile, Thermus Aquaticus", page 151 *
CHEMICAL ABSTRACTS, Vol. 105, No. 1, 7 July 1986, (Columbus, Ohio, US), A.K. FREY et al.: "Recovery of beta-Glactosidase by Adsorption from Unclarified Escherichia Coli Homogenate" see page 491 * Abstract 5024g & Eur. Congr. Biotechnol. 3rd, 1984, 1, 655-63* *
CHEMICAL ABSTRACTS, Vol. 85, No. 21, 22 November 1976, (Columbus, Ohio, US), A. CHIEN et al.: "Deoxyribonucleic Acid Polymerase from the Extreme Thermophile Thermus Aquaticus", see page 180* Abstract 155559t, & J. Bacteriol. 1976, 127 (3), 1550-7* *
CHEMICAL ABSTRACTS, Vol. 93, No. 5, 4 August 1980, (Columbus, Ohio, US), A.S. KALEDIN et al.: "Isolation and Properties of DNA Polymerase from Extremal Thermophylic Bacteria Thermus Aquaticus YT-1", see page 377* Abstract 40169P & Biokhimiya (Moscow) 1980, 45 (4), 644-51* *
CHEMICAL ABSTRACTS, Vol. 98, No. 7, 14 February 1983, (Columbus, Ohio, US), A.S. KALEDIN et al.: "Isolation and Properties of DNA Polymerase from the Extremely Thermophylic Bacteria Thermus Ruber", see page 298 * Abstract 49311q, & Biokhimiya (Moscow) 1982, 47 (11), 1785-91* *
Proc. Natl. Acad. Sci. USA, Vol. 85, December 1988 M.A. INNIS et al.: "DNA Sequencing with Thermus Aquaticus DNA Polymerase and direct Sequencing of Polymerase Chain Reaction-Amplified DNA", pages 9436-9440 *
Science, Vol. 239, 29 January 1988, R.K. SAIKI et al.: "Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase" pages 487-491 *

Also Published As

Publication number Publication date
IL88923A0 (en) 1989-08-15
IE72180B1 (en) 1997-03-26
EP0395736B2 (fr) 2006-08-23
IE890068L (en) 1989-07-12
DE68926038D1 (de) 1996-04-25
WO1989006691A2 (fr) 1989-07-27
SG46657A1 (en) 1998-02-20
EP0395736B1 (fr) 1996-03-20
JP2511548B2 (ja) 1996-06-26
EP0395736A1 (fr) 1990-11-07
ATE135741T1 (de) 1996-04-15
CA1341143C (fr) 2000-11-21
DE68926038T2 (de) 1996-10-17
ZA89262B (en) 1990-09-26
HK166096A (en) 1996-09-13
DE68926038T3 (de) 2007-02-01
AU3062989A (en) 1989-08-11
JPH03502165A (ja) 1991-05-23
IL88923A (en) 1995-07-31

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