WO2018139885A1 - Composition comprising acetoxychavicol acetate as active ingredient for preventing hair loss and promoting hair growth - Google Patents

Abstract

The present invention relates to a composition for preventing hair loss or promoting hair growth, comprising acetoxychavicol acetate as an active ingredient. More specifically, the present invention relates to a pharmaceutical composition, a quasi-drug composition, a cosmetic composition, and a health functional food for preventing hair loss or promoting hair growth, comprising acetoxychavicol acetate as an active ingredient. The acetoxychavicol acetate of the present invention has an excellent effect of accelerating the growth of hair follicles and thus, has an excellent effect of preventing hair loss and promoting hair growth; the acetoxychavicol acetate is also derived from natural products and thus has no side effects. Therefore, the acetoxychavicol acetate of the present invention can be variously utilized as a pharmaceutical composition, a quasi-drug composition, a cosmetic composition, and a health functional food for preventing hair loss or promoting hair growth.

Description

Hair loss prevention and hair growth promoting composition comprising acetoxychabicol acetate as an active ingredient

The present invention relates to a composition for preventing hair loss or promoting hair growth comprising acetoxychavicol acetate as an active ingredient.

Specifically, the present invention relates to a pharmaceutical composition, a quasi-drug composition, a cosmetic composition, and a food composition for preventing hair loss or promoting hair growth comprising acetoxychabicol acetate as an active ingredient.

Hair loss refers to a condition in which there is no hair in the area where hair should normally exist, and generally means that the hair of the scalp (coarse hair) falls out. Hair loss is a disease that occurs not only by aging but also by many factors in combination with young age. Specifically, the cause of hair loss may include internal factors such as genetic constitution and the action of testosterone, external stress such as daily stress, dietary habits, and accumulation of lipid peroxide in the scalp. In recent years, hair loss in young people, including obesity hair loss in women as well as male hair loss has been gradually spread, and many kinds of hair loss treatment agents, hair growth agents, etc. are commercially available to improve the hair loss phenomenon.

Minoxidil (minoxidil), a typical anti-hair loss treatment on the market, was originally developed for the treatment of hypertension, but it is a drug that has become more famous as a hair regrowth agent due to side effects of use. However, minoxidil preparations are known to cause side effects such as contact dermatitis or hirsutism, such as scalp pruritus, erythema, and keratin overuse (J Korean Med Assoc 2013 January; 56 (1): 45-54).

Another representative hair loss treatment agent, Propesia, blocks the 5-alpha-reductase type 2 enzyme, which plays an important role in the development of androgenetic alopecia, thereby inhibiting the change of testosterone to dihydrotestosterone (DHT). It is known to improve or inhibit the progression of hair loss. Propecia is known to have excellent hair loss prevention effects, but the reagents used to manufacture it are expensive and toxic, and because it acts in a way that artificially blocks the production of steroid hormones itself, many side effects may occur. There is a danger. Therefore, there is still a need for the development of a safe treatment agent having excellent efficacy of preventing hair loss and promoting hair growth, but having no side effects.

An object of the present invention is to provide a composition for preventing hair loss or promoting hair growth comprising acetoxychavicol acetate (acetoxychavicol acetate) as an active ingredient.

Specifically, an object of the present invention is to provide a pharmaceutical composition for preventing hair loss or promoting hair growth, quasi-drug composition, cosmetic composition and food composition comprising acetoxychabicol acetate as an active ingredient.

Pharmaceutical composition

The present invention provides a pharmaceutical composition for preventing hair loss or promoting hair growth comprising acetoxychavicol acetate (acetoxychavicol acetate) or a pharmaceutically acceptable salt thereof as an active ingredient.

[Formula 1]

In the present invention, the compound of Formula 1 may be chemically synthesized or separated from natural materials, and may be purchased and used commercially at home and abroad. In the present invention, when the compound of Formula 1 is separated from a plant, the type of plant is not particularly limited, but may be separated using a root diameter of Alpinia galanga . It is also possible to prepare plant extracts containing a high concentration of acetoxychabicol acetate.

The present inventors can prepare a high extract of acetoxychabicol acetate using the root of the mass steel ( Alpinia galanga, Thailand). In detail, according to the embodiment of the present invention, after cutting 50 g of the bulk rhizome living body, 250 ml of 70% aqueous ethanol was added and the ultrasonic extraction was repeated twice for 2 hours. After filtering the extract obtained by the above method, the filtrate was concentrated under reduced pressure at 40 ℃ ~ 50 ℃, and then lyophilized to give 2.2 g (yield 4.4%) of 70% ethanol extract of the mass root diameter. The acetoxychabicol acetate content in the obtained extract was found to be 14.61% (FIG. 3).

In the case of the experiment using the acetoxychabicol acetate single compound in the embodiment of the present invention was purchased from Abcam.

The compound of formula 1 of the present invention has the effect of promoting the growth of hair follicles.

Specifically, in the embodiment of the present invention, human hair follicle cells (n = 36) divided into four groups, the first group is a control (control) not treated with the drug, the second group is treated with testosterone 500 nM, the third group In the test group treated with 500 nM of silver testosterone and 100 nM of acetoxycarbicol acetate and 200 nM of testosterone and 200 nM of acetoxycarbicol acetate, hair follicles in the test group treated only with testosterone had a small size. While showing a reduction of more than 40%, both experimental groups treated with testosterone and acetoxychabicol acetate were confirmed to maintain the size of the hair follicles to a similar degree as the control group not treated with testosterone (FIG. 2). This demonstrates that the reduction in hair follicle size and thus hair growth inhibition by testosterone is restored by acetoxychabicol acetate.

The compound of formula 1 of the present invention has the effect of inhibiting the production of free radicals (ROS) in skin cells.

The compound of formula 1 of the present invention has the effect of inhibiting the activity of NADPH oxidase (Nox) in skin cells.

The present invention also provides a pharmaceutical composition for the prevention or treatment of hair loss comprising acetoxychavicol acetate (acetoxychavicol acetate) or a pharmaceutically acceptable salt thereof as an active ingredient as a compound represented by the following formula (1).

[Formula 1]

As used herein, the term pharmaceutically acceptable salt means a salt in a form that can be used pharmaceutically among salts in which cations and anions are bound by electrostatic attraction, and are generally metal salts and organic bases. Salts with, inorganic acids, salts with organic acids, salts with basic or acidic amino acids, and the like. For example, the metal salt may be an alkali metal salt (sodium salt, potassium salt, etc.), alkaline earth metal salt (calcium salt, magnesium salt, barium salt, etc.), aluminum salt, or the like; Salts with organic bases include triethylamine, pyridine, picoline, 2,6-lutidine, ethanolamine, diethanolamine, triethanolamine, cyclohexylamine, dicyclohexylamine, N, N-dibenzylethylenediamine Salts and the like; Salts with inorganic acids can be salts with hydrochloric acid, hydrobromic acid, nitric acid, sulfuric acid, phosphoric acid, and the like; Salts with organic acids can be salts with formic acid, acetic acid, trifluoroacetic acid, phthalic acid, fumaric acid, oxalic acid, tartaric acid, maleic acid, citric acid, succinic acid, methanesulfonic acid, benzenesulfonic acid, p-toluenesulfonic acid, and the like; Salts with basic amino acids can be salts with arginine, lysine, ornithine and the like; Salts with acidic amino acids can be salts with aspartic acid, glutamic acid and the like.

The pharmaceutical composition of the present invention may further include a pharmaceutically acceptable carrier in addition to including the compound of formula 1 or a pharmaceutically acceptable salt thereof as an active ingredient.

In the present invention, the type of the carrier is not particularly limited, and any carrier may be used as long as it is commonly used in the art. Non-limiting examples of the carrier include saline, sterile water, Ringer's solution, buffered saline, albumin injection solution, lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, maltodextrin, glycerol, ethanol, and the like. Can be. These may be used alone or in combination of two or more thereof.

In addition, the pharmaceutical composition of the present invention may be used, if necessary, by adding other pharmaceutically acceptable additives such as excipients, diluents, antioxidants, buffers or bacteriostatic agents, fillers, extenders, wetting agents, disintegrants, dispersants, surfactants , Binders or lubricants may be additionally added and used.

In the pharmaceutical composition of the present invention, the compound of Formula 1 or a pharmaceutically acceptable salt thereof may be included in an amount of 0.00001% to 99.99% by weight based on the total weight of the pharmaceutical composition, preferably 1% by weight. % To 99.99% by weight, more preferably 10% to 99.99% by weight, more preferably 50% to 99.99% by weight, and may also be included in the total content of the pharmaceutical composition.

The pharmaceutical compositions of the present invention can be formulated and used in a variety of formulations suitable for oral or parenteral administration.

Non-limiting examples of the formulation for oral administration include troches, lozenges, tablets, aqueous suspensions, oily suspensions, prepared powders, granules, emulsions, hard capsules, soft capsules, syrups or elixirs, and the like. Can be mentioned.

In order to formulate the pharmaceutical composition of the present invention for oral administration, a binder such as lactose, saccharose, sorbitol, mannitol, starch, amylopectin, cellulose or gelatin and the like; Excipients such as dicalcium phosphate and the like; Disintegrants such as corn starch or sweet potato starch; Lubricants such as magnesium stearate, calcium stearate, sodium stearyl fumarate or polyethylene glycol wax and the like can be used, and sweeteners, fragrances, syrups and the like can also be used. Furthermore, in the case of a capsule, a liquid carrier such as fatty oil may be additionally used in addition to the above-mentioned materials.

Non-limiting examples of the parenteral preparations include injection liquids, suppositories, respiratory inhalation powders, spray aerosols, ointments, application powders, oils, creams, and the like.

In order to formulate the pharmaceutical composition for parenteral administration, a sterile aqueous solution, a non-aqueous solvent, a suspension, an emulsion, a freeze-dried preparation, an external preparation, and the like may be used. The non-aqueous solvent and the suspension may be propylene glycol, Polyethyleneglycol, vegetable oils such as olive oil, injectable esters such as ethyloleate and the like can be used.

When formulating the pharmaceutical composition of the present invention into an injection solution, the composition of the present invention is mixed in water with a stabilizer or buffer to prepare a solution or suspension which is formulated for unit administration of ampoules or vials. can do.

When formulating the pharmaceutical composition of the present invention with an aerosol, a propellant or the like may be combined with the additive to disperse the dispersed dispersion or the wet powder.

When the pharmaceutical composition of the present invention is formulated into an ointment, a cream, a powder for application, an oil, an external skin preparation, etc., animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite , Silica, talc, zinc oxide and the like can be formulated using a carrier.

Pharmaceutically effective amounts, effective dosages of the pharmaceutical compositions of the present invention may vary depending on the formulation method, mode of administration, time of administration and / or route of administration of the pharmaceutical composition, and the reaction to be achieved by the administration of the pharmaceutical composition. The type and extent of the subject, type, age, weight, general state of health, condition and extent of the disease, sex, diet, excretion, and components of the drug or other composition used concurrently or simultaneously with the subject. And various similar factors well known in the medical arts, and those skilled in the art can readily determine and prescribe a dosage effective for the desired treatment.

Administration of the pharmaceutical composition of the present invention may be administered once a day, may be divided into several times. The pharmaceutical compositions of the present invention may be administered as individual therapeutic agents or in combination with other therapeutic agents and may be administered sequentially or simultaneously with conventional therapeutic agents. All of the above factors can be considered and administered in an amount that can obtain the maximum effect in a minimum amount without side effects, which can be easily determined by those skilled in the art.

The route of administration and mode of administration of the pharmaceutical compositions of the present invention may be independent of each other, and may be any route of administration and mode of administration without particular limitation, so long as the pharmaceutical composition can reach the desired site of interest. The pharmaceutical composition may be administered by oral or parenteral administration.

As the parenteral administration method, intravenous administration, intraperitoneal administration, intramuscular administration, transdermal administration or subcutaneous administration may be used, and the method of applying, spraying or inhaling the composition to a diseased site may also be used, but the present invention is not limited thereto. It doesn't work.

The pharmaceutical composition of the present invention may be preferably used in a method of directly applying or spreading on hair, skin or scalp.

As used herein, the term “hair” may include all parts of the hair root and hair follicles throughout the body, such as hair roots and hair follicles of hair, hair, eyelashes, eyelashes, and beards.

Quasi-drug composition

The present invention provides a quasi-drug composition for preventing hair loss or promoting hair growth comprising acetoxychabicol acetate, or a pharmaceutically acceptable salt thereof, as a compound represented by the following Formula 1.

[Formula 1]

As used herein, the term “quasi drug” refers to articles that have a lesser action than pharmaceuticals among articles used for the purpose of diagnosing, treating, ameliorating, alleviating, treating, or preventing a disease in humans or animals. According to the article, quasi-drugs exclude products used for the purpose of medicines, and include products used for the treatment or prevention of diseases of humans and animals, and products with slight or no direct action on the human body.

When the compound of Formula 1 of the present invention is used as an quasi-drug additive, it may be added as it is or used with other quasi-drug or quasi-drug components, and may be appropriately used according to a conventional method. The mixing ratio with other ingredients used together may be suitably determined depending on the purpose of use (eg, prevention, improvement or therapeutic treatment).

Examples of the quasi-drug composition may include, but are not limited to, scalp hair care compositions such as shampoos and rinses, body cleansers, foams, soaps, masks, ointments, creams, lotions, essences, sprays, and the like.

The quasi-drug composition comprising the compound of formula 1 of the present invention or a pharmaceutically acceptable salt thereof as an active ingredient may preferably be a composition for scalp hair care.

As used herein, the term “composition for scalp hair care” refers to a composition that prevents or alleviates damage to the scalp and hair. The formulation of the scalp hair care composition may be, but is not limited to, shampoo, rinse, essence, nutrient, serum, massage cream, lotion, pack, and the like.

The compound of Formula 1 or a pharmaceutically acceptable salt thereof may be included in an amount of about 0.001 to 50% by weight, preferably about 10% by weight, based on the total weight of the quasi-drug composition.

Cosmetic composition

The present invention provides a cosmetic composition for preventing hair loss or promoting hair growth comprising acetoxychabicol acetate or a cosmetically acceptable salt thereof as an active ingredient, which is a compound represented by Formula 1 below.

[Formula 1]

As used herein, the term “cosmetically acceptable salts” refers to salts that can be used cosmetically even among salts in which cations and anions are bonded by electrostatic attraction. Specific examples include examples of the "pharmaceutically acceptable salts" described above.

Formulation of the cosmetic composition of the present invention is, for example, solution, shampoo, rinse, external ointment, cream, foam, nourishing lotion, flexible lotion, pack, flexible water, latex, makeup base, essence, liquid cleanser, bath, sun Screen creams, sun oils, suspensions, emulsions, pastes, gels, lotions, powders, soaps, surfactant-containing cleansing, oils, powder foundations, emulsion foundations, wax foundations, patches, sprays, and the like. Do not.

The cosmetic composition of the present invention may further include one or more cosmetically acceptable carriers formulated in general cosmetics, and as common components, for example, oil, water, surfactants, moisturizers, lower alcohols, thickeners, Chelating agents, pigments, preservatives, fragrances and the like can be appropriately blended, but is not limited thereto.

The cosmetically acceptable carrier included in the cosmetic composition of the present invention may vary depending on the formulation of the cosmetic composition.

When the formulation of the present invention is an ointment, paste, cream or gel, the carrier component is animal oil, vegetable oil, wax, paraffin, starch, tragacanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide Etc. may be used, but is not limited thereto. These may be used alone or in combination of two or more thereof.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder, and the like can be used, in particular, in the case of a spray, additionally chlorofluorohydrocarbons. Propellant, such as, but not limited to, propane / butane or dimethyl ether. These may be used alone or in combination of two or more thereof.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer or emulsion may be used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, Propylene glycol, 1,3-butylglycol oil, and the like can be used, and in particular, cottonseed oil, peanut oil, corn seed oil, olive oil, castor oil and sesame oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid ester May be used, but is not limited thereto. These may be used alone or in combination of two or more thereof.

When the dosage form of the present invention is a suspension, liquid carrier diluents such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, micro Crystalline cellulose, aluminum metahydroxyde, bentonite, agar or tragacanth may be used, but is not limited thereto. These may be used alone or in combination of two or more thereof.

When the formulation of the present invention is a soap, alkali metal salts of fatty acids, fatty acid hemiester salts, fatty acid protein hydrolyzates, isethionates, lanolin derivatives, aliphatic alcohols, vegetable oils, glycerol, sugars and the like are used as carrier components. It may be, but is not limited thereto. These may be used alone or in combination of two or more thereof.

The cosmetic composition of the present invention may be preferably a cosmetic composition for scalp hair care. Specifically, hair tonic, hair cream, hair lotion, hair shampoo, hair rinse, hair conditioner, hair spray, hair aerosol, pomade, powder, gel, hair pack, hair treatment, eyebrow regrowth, eyelash regrowth, eyelash nutrition But it is not limited thereto.

The compound of Formula 1 or a cosmetically acceptable salt thereof is not limited in content, but may be included in an amount of 0.001 to 50% by weight, preferably 10% by weight, based on the total weight of the cosmetic composition.

Food composition

The present invention provides a food composition for preventing hair loss or promoting hair growth comprising acetoxychabicol acetate, a compound represented by the following Formula 1, or a food acceptable salt thereof as an active ingredient.

[Formula 1]

The present invention also provides a food composition for preventing or improving hair loss comprising acetoxychabicol acetate, a compound represented by the formula (1), or a food acceptable salt thereof as an active ingredient.

The compound of formula 1 of the present invention can be used as a food composition because it is derived from natural products and has been proven to be stable. The compound of Formula 1 or a food acceptable salt thereof is not limited in content, but may be included in an amount of about 0.001 to 50% by weight, preferably about 10% by weight, based on the total weight of the food composition. In addition, the composition may include additional ingredients that are commonly used in food compositions to improve the smell, taste, time and the like. For example, it may include vitamins A, C, D, E, B1, B2, B6, B12, niacin, biotin, folate, panthotenic acid, and the like. In addition, it may include minerals such as zinc (Zn), iron (Fe), calcium (Ca), chromium (Cr), magnesium (Mg), manganese (Mn), copper (Cu). It may also contain amino acids such as lysine, tryptophan, cysteine, valine and the like. Also preservatives (potassium sorbate, sodium benzoate, salicylic acid, sodium dihydroacetate, etc.), fungicides (bleaching powder and highly bleaching powder, sodium hypochlorite, etc.), antioxidants (butylhydroxyanisol (BHA), butylhydroxytoluene (BHT) ), Colorants (such as tar colors), colorants (such as sodium nitrite, sodium nitrite), bleach (sodium sulfite), seasonings (such as MSG glutamate), sweeteners (ducin, cyclate, saccharin, sodium, etc.), Food additives such as fragrances (vanillin, lactones, etc.), swelling agents (alum, potassium D-tartrate, etc.), reinforcing agents, emulsifiers, thickeners (pigments), coatings, gum herbicides, foam inhibitors, solvents, and improving agents. Can be added. The additive is selected according to the type of food and used in an appropriate amount.

As used herein, the term food acceptable salt means a salt in a form that can be used in food, even among salts in which cations and anions are bound by electrostatic attraction, and specific examples of the types thereof Includes examples of the “pharmaceutically acceptable salts” described above.

The content of the compound represented by Formula 1 in the food composition of the present invention may be appropriately determined depending on the intended use. In general, the compound represented by Formula 1 may be added at 0.0001 to 100% by weight based on the total weight of the food composition.

In the present invention, there is no particular limitation on the form of the food composition, and includes all foods in the usual sense. Foods to which the present invention can be applied include, for example, beverages, drinks, and health functional foods.

As used herein, the term "health functional food" means a food having an active health maintenance or promotion effect compared to a general food, and a health supplement food means a food for health supplement purposes. In some cases, the terms functional food, health food, health supplement food are used in. The food may be prepared in various forms such as tablets, capsules, powders, granules, liquids, pills, etc. in order to obtain useful effects.

The term used in the present invention, functional food (functional food) is the same term as a food for special health use (FOSHU), in addition to nutrition, medical, medical effect processed to appear efficiently to regulate the bioregulatory function Means high food.

As a specific example of the health functional food of the present invention, by using the compound of Formula 1 or a food-acceptable salt thereof, it is possible to prepare a processed food with improved storage characteristics while modifying the characteristics of agricultural products, animal products or aquatic products. The dietary supplement is not particularly limited, but preferably margarine, fat continuous or water continuous or both continuous spreads, fat reduced spreads, chocolate, or chocolate coating or chocolate loaf. (fillings) or sweets such as bakery, ice cream, ice cream coatings, ice cream ingredients, dressings, mayonnaise, cheese, cream substitutes, dry soups, drinks, cereal bars, sauces, snack bars, dairy products, clinical nutrition foods, pediatric foods It may be used prepared in the form.

Hair loss prevention or hair growth promoting composition comprising the compound of the formula (1) of the present invention as an active ingredient can be used for other animals, especially pets other than humans by appropriately modifying the formulation.

Use of the composition of the present invention, the method of preventing, ameliorating or treating hair loss using the composition of the present invention

The present invention provides the use of the pharmaceutical composition described above for the preparation of a medicament for the prevention, treatment or amelioration of hair loss, or for the promotion of hair growth.

The present invention also provides the use of the aforementioned pharmaceutical, quasi-drug, cosmetic or food composition for the prevention, treatment or amelioration of hair loss, or the promotion of hair growth.

The present invention also provides a method for treating hair loss, comprising administering to a subject in need thereof a therapeutically effective amount of the above-mentioned pharmaceutical. In the present invention the subject comprises a mammal, in particular a human.

Acetoxychabicol acetate of the present invention has an excellent effect of promoting the growth of hair follicles to prevent hair loss and promote hair growth, and is derived from natural products and has no side effects, thus preventing hair loss or promoting hair growth. It can be used in various ways as a composition, quasi-drug composition, cosmetic composition, and food composition.

1 is a photograph observed through the animal experiments of the hair loss prevention and hair growth promoting effect of the acetoxychabicol acetate of the present invention.

Figure 2 is a graph illustrating the hair follicle growth promoting effect of the acetoxychabicol acetate of the present invention in human hair follicle cell organ culture.

3 is an HPLC chromatogram of 70% ethanol extract of Alpinia galanga root.

Figure 4 is a photograph and graphs observed through animal experiments to prevent hair loss and hair growth promoting effect of the mass extract.

Hereinafter, the present invention will be described in more detail with reference to Examples. However, these examples are for illustrative purposes only and the scope of the present invention is not limited to these examples.

Example 1 Hair Loss Prevention and Hair Growth Promoting Effect of Acetoxychabicol Acetate

In order to confirm the hair loss prevention and hair growth promoting effect of acetoxychabicol acetate, the following animal experiment was performed. Acetoxychabicol acetate used in this example was purchased from Abcam.

Seven 7-week-old C57BL / 6 mice were divided into three experimental groups, in which all three groups received testosterone (10 μM solution, 100 μl foam) daily on mouse back skin, but the first group applied only testosterone and the second In group 3 and 3, acetoxychabicol acetate (10 μM solution, 50 μl foam) or minoxidil (2% solution, 50 μl foam) was applied to the back skin with testosterone from day 3, respectively. The experiment was conducted for 17 days based on the testosterone application date.

As a result of the test, hair loss progressed gradually in mice treated only with testosterone, and it was observed that almost 17 days of testosterone treatment showed little hair loss on the back skin. In contrast, in the case of mice coated with acetoxycarbicol acetate, hair loss occurred slightly at first, but the density of hair remained significantly higher than that of the testosterone-treated mice. On the 14th day, the hair density increased again. This demonstrates that acetoxychabicol acetate is effective in preventing hair loss by testosterone and at the same time promoting hair growth (FIG. 1).

In addition, acetoxychabicol acetate was confirmed to exhibit a similar degree of hair loss prevention and hair growth promoting effect compared to the effect of minoxidil (MNX) currently used as a hair loss treatment (FIG. 1). Therefore, acetoxychabicol acetate is expected to be used in various ways as a treatment for hair loss because it shows similar or more hair loss prevention and hair growth promoting effects with minoxidil, and there is no risk of side effects derived from natural products.

Example 2: Hair follicle growth promoting effect of acetoxychabicol acetate

In order to confirm the hair follicle growth promoting effect of acetoxychabicol acetate, the following human hair follicle cell organ culture experiments were performed. Acetoxychabicol acetate used in this example was purchased from Abcam.

With the consent of the patients undergoing hair transplantation, the hair was separated from the scalp tissue, and the lower part of the sebaceous gland was cut to date and used for the lower part of hair. Tissues were maintained in Williams E medium supplemented with 100 U penicillin / streptomycin, 10 ng / ml hydrocortisone, 10 ug / ml insulin and 2 mM L-glutamin under conditions of 5% CO2 and 37 ° C. 500 nM testosterone and each drug Was treated in the medium for each concentration and 9 tissues were added and cultured for each condition. Change the medium every 3 days and measure the length of tissue growth after 6 days. The human hair follicle cells (n = 36) were divided into four groups, the first group was a drug-free control group, the second group was treated with testosterone 500 nM, and the third group was testosterone 500 nM and acetoxychabi. Treatment with 100 nM of call acetate, the fourth group was treated with 500 nM of testosterone and 200 nM of acetoxychabicol acetate to measure hair length growing in hair follicle cells. As a result, the test group treated only with testosterone showed a hair follicle-producing hair of about 40% or more, while the test group treated with testosterone and acetoxychabicol acetate together had a similar degree to the control group without testosterone. It was confirmed that hair follicle generated hair length was maintained (FIG. 2). This demonstrates that hair follicle-producing hair growth inhibition by testosterone is restored by acetoxychabicol acetate.

Example 3: Preparation of Bulk Steel Extract Containing High Concentration of Acetoxychabicol Acetate

Extracts containing high concentrations of acetoxychabicol acetate were prepared using commercial bulk steels ( Alpinia galanga, Thailand) as follows.

Specifically, after cutting 50 g of large diameter rhizome biomass, 250 ml of 70% aqueous ethanol was added and the ultrasonic extraction was repeated twice for 2 hours. After filtering the extract obtained by the above method, the filtrate was concentrated under reduced pressure at 40 ℃ ~ 50 ℃, and then lyophilized to obtain 2.2 g (yield 4.4%) of 70% ethanol extract of the mass root diameter. The acetoxychabicol acetate content in the obtained extract was found to be 14.61% (FIG. 3).

Example 4: Hair loss prevention and hair growth promoting effect of the mass extract

Animal experiments were performed using the bulk steel extract prepared in Example 3 as follows.

Remove all dorsal hairs from two 8-week-old C57BL / 6 mice, divide them into two groups, and apply testosterone (2 mg / ml solution, 100 μl foam) to the back of the mouse daily in both groups. Only testosterone was applied, and the second group applied the bulk steel extract prepared in Example 3 to the back skin along with testosterone. The experiment was conducted for 7 days based on the testosterone application date. After 7 days the condition and hair follicle size of the mouse back skin hair were measured.

As a result of the test, only testosterone was almost lost to the hair of the back of the mouse, whereas in the case of mice coated with a mass river extract, hair growth was promoted on the back of the hair which had been lost, thereby increasing the hair density. . In addition, the size of the hair follicles also showed superior growth compared to the case of applying only testosterone, confirming the hair loss prevention and hair growth promoting effect of the bulk steel extract containing high concentration of acetoxychabicol acetate (FIG. 4).

In the present specification, those skilled in the art of the present invention can fully recognize and infer the details that have been omitted, and the technical spirit or essential configuration of the present invention in addition to the specific examples described in this specification are changed. Many more variations are possible without departing. Therefore, the present invention may be implemented in a manner different from that specifically described and illustrated herein, which can be understood by those skilled in the art.

Claims (10)

1. A pharmaceutical composition for preventing hair loss or promoting hair growth comprising a compound represented by the following Formula 1 or a pharmaceutically acceptable salt thereof as an active ingredient.

   [Formula 1]

   
2. The pharmaceutical composition of claim 1, wherein the composition has an effect of promoting hair follicle growth.
3. A quasi-drug composition for preventing hair loss or promoting hair growth comprising a compound represented by the following Formula 1 or a cosmetically acceptable salt thereof as an active ingredient.

   [Formula 1]

   
4. The quasi-drug composition according to claim 3, wherein the composition has an effect of promoting hair follicle growth.
5. A cosmetic composition for preventing hair loss or promoting hair growth comprising a compound represented by the following Formula 1 or a cosmetically acceptable salt thereof as an active ingredient.

   [Formula 1]

   
6. The cosmetic composition according to claim 5, wherein the composition has an effect of promoting the growth of hair follicles.
7. A food composition for preventing hair loss or promoting hair growth comprising a compound represented by the following Formula 1 or a food acceptable salt thereof as an active ingredient.

   [Formula 1]

   
8. The food composition of claim 7, wherein the food composition has an effect of promoting the growth of hair follicles.
9. The use of a pharmaceutical composition according to claim 1 for the manufacture of a medicament for the prevention, treatment or amelioration of hair loss is provided.
10. A method of treating hair loss comprising administering to a subject in need thereof a pharmaceutical composition in accordance with claim 1 in a therapeutically effective amount.

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