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WO2018105919A2 - Boisson à la grenade contenant du collagène marin et son procédé de fabrication - Google Patents

Boisson à la grenade contenant du collagène marin et son procédé de fabrication Download PDF

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Publication number
WO2018105919A2
WO2018105919A2 PCT/KR2017/013121 KR2017013121W WO2018105919A2 WO 2018105919 A2 WO2018105919 A2 WO 2018105919A2 KR 2017013121 W KR2017013121 W KR 2017013121W WO 2018105919 A2 WO2018105919 A2 WO 2018105919A2
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WO
WIPO (PCT)
Prior art keywords
weight
parts
collagen
pomegranate
marine collagen
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Application number
PCT/KR2017/013121
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English (en)
Korean (ko)
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WO2018105919A3 (fr
Inventor
황재호
Original Assignee
주식회사 마린테크노
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Application filed by 주식회사 마린테크노 filed Critical 주식회사 마린테크노
Publication of WO2018105919A2 publication Critical patent/WO2018105919A2/fr
Publication of WO2018105919A3 publication Critical patent/WO2018105919A3/fr

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Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Preparation or treatment thereof
    • A23L2/02—Non-alcoholic beverages; Dry compositions or concentrates therefor; Preparation or treatment thereof containing fruit or vegetable juices
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Preparation or treatment thereof
    • A23L2/52—Adding ingredients
    • A23L2/66—Proteins

Definitions

  • the present invention relates to a pomegranate drink containing marine collagen and a method for producing the same, and a drink containing a marine collagen extracted from fish and a pomegranate useful for health and a method for producing the same.
  • Collagen is the most abundant protein in the animal body, accounting for more than 30% of body protein, and has been found to be at least 19 types (Type I-III) (Nakamura, YN et al., Relationship among collagen amount, distribution). and architecture in the M. Longissimus thoracis and M. pectoralis profundus from pigs.Meat Science, 64, pp 43-50, 2003).
  • collagen is a major protein of animal connective tissue, and supports tissues and organs and surrounds the body surface and plays a role in maintaining body shape.
  • connective tissue such as skin, cartilage and bone in the living body, 40% is widely distributed throughout the body, such as skin, 20% bone and cartilage, and other blood vessels and internal organs.
  • collagen has a triple helix structure, about 14-15 nm in diameter, 280-300 nm in length, and an average molecular weight of about 300 KDa (Lehninger, AL Biochemistry, 2nd ed., Pp. 145, 1975), have a regular sequence of amino acids such as (Gly-XY) n and share within or between tropocollagen molecules, the basic molecules of fibrous proteins.
  • Physically and biologically stable structures are formed by binding crosslinking (McClain, PE et al., Amino acid composition and cross-linking characteristics of collagen intramuscular connective tissue of striated muscle (Bos taurus). of Biochemistry, 2 (7), pp 121-124, 1971).
  • Collagen has been applied as a raw material of leather or gelatin for a long time, and its field of application has become more diverse in recent years. In the food industry, it is used for meat packaging materials such as sausage and salami as an edible casing raw material. Collagen applied to medicines has been reported to have a healing effect on skin damaged by burns or wounds (Jeyanthi, R. et al., Solid tumour chemotherapy using implantable collagen-poly (HEMA) hydrogel containing 5-fluorouracil. of Pharmacy & Pharmacology, 43, pp. 60-62, 1991). As such, collagen is not only used as a functional material for foods and medicines, but also has a function of enhancing skin moisturizing, and thus is widely used in various fields such as basic materials of cosmetics.
  • HEMA implantable collagen-poly
  • Collagen hydrolyzate refers to a polymer that has been extracted from donpi, and then hydrolyzed through post-treatment such as enzymatic digestion to reduce the molecular weight into peptides. Are sold.
  • Patent Document 1 Republic of Korea Patent No. 10-0775055: Health functional beverage containing collagen extracted from deer antler and its manufacturing method
  • the present invention has been made to improve the above problems, and an object thereof is to provide a beverage containing marine collagen extracted from fish and pomegranate useful for health, and a method for producing the same.
  • Pomegranate drink containing marine collagen of the present invention for achieving the above object is 1 to 10 parts by weight of pomegranate concentrate, 0.5 to 5 parts by weight of marine collagen extracted from fish, 10 to 15 white sugar based on 100 parts by weight of purified water It contains weight part, 2-10 weight part of oligosaccharides, 0.1-0.6 weight part of citric acid, and 0.05-0.5 weight part of vitamin C.
  • the pomegranate drink further contains 1 to 10 parts by weight of sageumchocho extract based on 100 parts by weight of the purified water.
  • a method for producing a pomegranate drink containing marine collagen of the present invention for achieving the above object comprises a concentration step of obtaining a pomegranate concentrate by concentrating the pomegranate juice; A collagen extraction step of extracting marine collagen from fish; 1 to 10 parts by weight of the pomegranate concentrate, 0.5 to 5 parts by weight of marine collagen, 10 to 15 parts by weight of white sugar, 2 to 10 parts by weight of oligosaccharide, 0.1 to 0.6 parts of citric acid, Stirring the mixture containing the vitamin C 0.05 to 0.5 parts by weight; And a heating step of heating the mixture at 83 to 87 ° C. for 10 to 20 minutes after the stirring.
  • the collagen extraction step is a) adding a sodium hydroxide solution to the skin powder to obtain an alkali residue from which the non-collagenic protein has been removed, b) adding an acetic acid solution to the alkali residue and stirred and separating the supernatant with a centrifuge Extracting acid-solubilized collagen, c) adding pepsin to the acid-solubilized collagen, stirring, separating the supernatant with a centrifugal separator, and then adding the sodium chloride solution to dialysis the precipitate precipitated with distilled water to extract pepsin-solubilized collagen and and d) purifying the pepsin solubilized collagen.
  • weedelia based on 100 parts by weight of the purified water prostrata ) 1 to 10 parts by weight of the extract is further combined and stirred.
  • the present invention as described above can provide a beverage containing a marine collagen extracted from fish and pomegranate useful for health and a preparation method thereof.
  • the present invention has the advantage that can be used as a useful resource by the waste skin by-products.
  • Pomegranate drink according to an embodiment of the present invention is 1 to 10 parts by weight of pomegranate concentrate, 100 to 5 parts by weight of purified water, 0.5 to 5 parts by weight of marine collagen extracted from fish, 10 to 15 parts by weight of white sugar, 2 to 10 oligosaccharides It contains a weight part, 0.1-0.6 weight part of citric acid, and 0.05-0.5 weight part of vitamin C.
  • Pomegranate drink according to another embodiment of the present invention further contains the sageumchocho.
  • pomegranate concentrate for example, 1 to 10 parts by weight of pomegranate concentrate, 0.5 to 5 parts by weight of marine collagen extracted from fish, 1 to 10 parts by weight of seaweed fireweed extract, 10 to 15 parts by weight of white sugar, oligosaccharide 2 To 10 parts by weight, citric acid 0.1 to 0.6 parts by weight, and vitamin C 0.05 to 0.5 parts by weight.
  • Pomegranate drink of the present invention is well known food additives may be added.
  • food additives include sugars such as monosaccharides, disaccharides, polysaccharides, and sugar alcohols, flavoring agents such as taumartin, stevia extract, saccharin, and aspartame, nutrients, vitamins, edible electrolytes, flavors, coloring agents, and enhancers (e.g., cheese). , Chocolate, etc.), pectic acid, alginic acid, organic acid, protective colloid thickener, pH adjuster, stabilizer, preservative, glycerin, alcohol, carbonation agent and the like can be used.
  • Method for producing a pomegranate beverage is a concentration step of obtaining a pomegranate concentrate by concentrating the pomegranate juice, a collagen extraction step of extracting marine collagen from fish, and the pomegranate concentrate 1 with respect to 100 parts by weight of purified water To 10 parts by weight, 0.5 to 5 parts by weight of the marine collagen, 10 to 15 parts by weight of white sugar, 2 to 10 parts by weight of oligosaccharide, 0.1 to 0.6 parts by weight of citric acid, and 0.05 to 0.5 parts by weight of vitamin C And a stirring step of stirring, and a heating step of heating the mixture at 83 to 87 ° C. for 10 to 20 minutes after stirring, and packaging the mixture after heating. Look specifically at each step.
  • Pomegranate juice is concentrated to obtain a pomegranate concentrate.
  • Pomegranate juice is obtained by juice of the pomegranate fruit. And pomegranate juice is concentrated using a concentrator to obtain a pomegranate concentrate.
  • the pomegranate juice may be concentrated to a concentration of about 50 to 80 Brix using a rotary vacuum concentrator to obtain a pomegranate concentrate.
  • marine collagen is extracted from fish, a byproduct of fish.
  • Fish skin can be used as a shell of fish such as halibut, shellfish, sea bass, red snapper, salmon and the like.
  • the collagen extraction step is a) adding sodium hydroxide solution to the skin powder to obtain an alkali residue from which the non-collagenic protein has been removed, b) adding an acetic acid solution to the alkali residue, stirring and separating the supernatant with a centrifuge Extracting solubilized collagen, c) adding pepsin to the acid-solubilized collagen, stirring and separating the supernatant with a centrifuge, and then extracting pepsin solubilized collagen by dialysis of the precipitate precipitated by adding sodium chloride solution; d) purifying the pepsin solubilized collagen.
  • Skin is washed, dried and then pulverized to a size of 50 to 150 mesh to prepare the skin powder. Predrying may be performed to remove scales on the skin prior to drying. To do this, the washed fish is mixed with 0.1 M sodium hydroxide solution and stirred for 6 to 12 hours. When the mixture is stirred with sodium hydroxide solution, there are some differences depending on the type of fish, but 80 to 90% of scales are easily removed.
  • the fish powder and sodium hydroxide solution were mixed at a weight ratio of 1: 5 to 10, and then stirred at room temperature (20 to 25 ° C.) for 12 to 24 hours to obtain an alkali residue from which the non-collagenic protein was removed using a centrifuge. can do.
  • the alkaline residue is washed with distilled water, and then acetic acid is added to extract collagen.
  • Alkaline residue and acetic acid solution are mixed at a weight ratio of 1: 5 to 10, and then stirred at room temperature (20 to 25 ° C) for 12 to 24 hours, and the supernatant is separated using a centrifuge to obtain acid solubilized collagen.
  • pepsin was added to the acid-solubilized collagen, stirred for 10 to 20 hours, the supernatant was separated by centrifugation, and the precipitate precipitated by adding 2 M sodium chloride solution was dialyzed with distilled water to obtain pepsin solubilized collagen.
  • pepsin solubilized collagen was dialyzed at 20 mM Na 2 HPO 4 to inactivate pepsin and then dialyzed against 50 mM acetic acid solution containing urea (pH 4.8), followed by ion chromatography. Fractions are used to elute. For example, purification was performed with a linear gradient (60 ml / h) of 0-600 mM NaCl in a column filled with cellulose phosphate (P11, Whatman, Maidstone, UK), and the fraction eluted at 230 nm was 0.5 containing 2.0 M NaCl. Dialysis with M acetate solution is recovered, followed by dialysis and freeze-drying with distilled water to obtain high purity marine collagen.
  • pomegranate concentrate, marine collagen, additives and the like are mixed with purified water and stirred.
  • pomegranate concentrate 0.5 to 5 parts by weight of marine collagen, 10 to 15 parts by weight of white sugar, 2 to 10 parts by weight of oligosaccharide, 0.1 to 0.6 parts of citric acid, , 0.05 to 0.5 parts by weight of vitamin C is blended. The mixture so blended is stirred so that the whole is uniformly mixed.
  • food additives may be further added in the stirring step.
  • food additives include sugars such as monosaccharides, disaccharides, polysaccharides, and sugar alcohols, flavoring agents such as taumartin, stevia extract, saccharin, and aspartame, nutrients, vitamins, edible electrolytes, flavors, coloring agents, and enhancers (e.g., cheese). , Chocolate, etc.), pectic acid, alginic acid, organic acid, protective colloid thickener, pH adjuster, stabilizer, preservative, glycerin, alcohol, carbonation agent and the like can be used.
  • another example of the present invention may be further mixed by mixing 1 to 10 parts by weight of jinbul fireweed extract in the stirring step.
  • 1 to 10 parts by weight of pomegranate concentrate 0.5 to 5 parts by weight of marine collagen extracted from fish, 1 to 10 parts by weight of seaweed fireweed extract, 10 to 15 parts by weight of white sugar, oligosaccharide 2
  • citric acid 0.1 to 0.6 parts by weight
  • vitamin C 0.05 to 0.5 parts by weight.
  • Wedelia prostrata is a perennial herb that grows on the beach, and young sprouts are eaten in the form of herbs. Mudweed uses leaves.
  • Various extracting methods can be applied to obtain the sageumchocho extract. It can be obtained by adding an extraction solvent as one of the extraction methods. At least one selected from water, a lower alcohol having 1 to 4 carbon atoms, a polyhydric alcohol, or a mixture thereof may be used as the extraction solvent. Methanol, ethanol, etc. can be used as a C1-C4 lower alcohol, Butylene glycol, propylene glycol, pentylene glycol, etc. can be used as a polyhydric alcohol. And the mixture may be a mixture of water and lower alcohol, a mixture of water and polyhydric alcohol, a mixture of lower alcohol and polyhydric alcohol, or a mixture of water and lower alcohol and polyhydric alcohol.
  • the extraction solvent in the weight ratio with respect to the loose leaf buds can be used for hot water extraction, cold needle or hot needle extraction for 10 to 150 °C for 1 to 48 hours. After extraction, the resultant is filtered to obtain liquid mudweed.
  • Saenggeum fireweed is a salty plant that contains a variety of minerals and can increase the functionality of pomegranate drink.
  • sageumchocho extract is excellent in antioxidant activity, increasing the long-term preservation of pomegranate drink and is useful for health.
  • the mixture After stirring the mixture is heat treated at 83-87 ° C. for 10-20 minutes. The heat treatment makes the mixture evenly and sterilized at the same time.
  • the mixture After heating, the mixture can be packed by packing the packaging.
  • Pouches, spout pouches, and PET can be used for packaging to maintain simplicity and quality in the distribution process.
  • Pomegranate juice obtained by juice of the pomegranate fruit was concentrated to a concentration of about 60 Brix using a rotary vacuum concentrator to prepare a pomegranate concentrate.
  • Marine collagen was prepared by purchasing commercialized powder products (fish collagen, geltec, Korea).
  • Pomegranate drink was prepared by heating at 15 ° C.
  • the sageumchocho extract was added 10 times by weight of water to the sageumcho leaf and then extracted with hot water at 90 ° C. for 8 hours and then filtered through a filter paper to use as a liquid extract.
  • Antioxidant activity was tested using the fermented beverages of Examples 1 and 2 as samples.
  • Radical scavenging ability (absorbance of 1-sample addition / absorption of no addition) x 100
  • Example 2 Referring to the results of Table 1, the radical scavenging ability of Example 2 was much higher than that of Example 1. This seems to be due to the antioxidant effect of sageumchocho.
  • the pomegranate drink added with the lotus leaf bud is expected to be useful for preventing the aging and preventing various diseases when it is ingested as a food.

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  • Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Non-Alcoholic Beverages (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)

Abstract

La présente invention concerne une boisson à la grenade contenant du collagène marin et son procédé de fabrication et, plus particulièrement, une boisson contenant du collagène marin extrait de sous-produits de poisson et de grenades bénéfiques pour la santé, et son procédé de fabrication. La boisson à la grenade contenant du collagène marin selon la présente invention contient de 1 à 10 parts en poids d'un concentré de grenade, de 0,5 à 5 parts en poids de collagène marin extrait de poisson, de 10 à 15 parts en poids de sucre blanc, de 2 à 10 parts en poids d'un oligosaccharide, de 0,1 à 0,6 parts en poids d'acide citrique, et 0,05 à 0,5 parts en poids de vitamine C.
PCT/KR2017/013121 2016-12-07 2017-11-17 Boisson à la grenade contenant du collagène marin et son procédé de fabrication WO2018105919A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020160165700A KR101883979B1 (ko) 2016-12-07 2016-12-07 마린 콜라겐을 함유한 석류음료 및 이의 제조방법
KR10-2016-0165700 2016-12-07

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WO2018105919A2 true WO2018105919A2 (fr) 2018-06-14
WO2018105919A3 WO2018105919A3 (fr) 2018-08-09

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KR102129097B1 (ko) 2020-01-08 2020-07-01 김영환 정제수가 포함되지 않은 농축과즙젤리 포장 방법
KR102129087B1 (ko) 2020-01-08 2020-07-01 김영환 정제수가 포함되지 않은 농축과즙젤리 가공 방법
KR20220162211A (ko) 2021-05-31 2022-12-08 이주연 인체 대사 촉진 체온 추출법에 의한 피부 맑음탕 제조방법

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KR100775055B1 (ko) 2006-10-31 2007-11-09 김도완 녹용에서 추출한 콜라겐을 함유하는 건강 기능성 음료 및그 제조 방법
KR20090087716A (ko) * 2008-02-13 2009-08-18 이종남 고농도 콜라겐 함유 농축형 식초 음료 제조방법
KR20100128792A (ko) * 2009-05-29 2010-12-08 주식회사오뚜기 피쉬 콜라겐을 함유하는 젤리형 드레싱 조성물 및 그 제조방법
KR101341704B1 (ko) * 2013-05-02 2013-12-16 황재호 부산물을 이용한 콜라겐 제조 방법
KR101609197B1 (ko) * 2014-03-17 2016-04-06 좋은영농조합법인 어류 콜라겐 음료의 제조방법
KR101700927B1 (ko) * 2015-04-09 2017-01-31 주식회사 마린테크노 고순도 마린콜라겐의 제조 방법
CN105901743A (zh) * 2016-05-12 2016-08-31 长青（中国）日用品有限公司 一种石榴枸杞胶原蛋白固体饮料

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KR101883979B1 (ko) 2018-07-31
WO2018105919A3 (fr) 2018-08-09
KR20180065188A (ko) 2018-06-18

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