+

WO2018186682A1 - Substrat de biocapteur, son procédé de production, et biocapteur le comprenant - Google Patents

Substrat de biocapteur, son procédé de production, et biocapteur le comprenant Download PDF

Info

Publication number
WO2018186682A1
WO2018186682A1 PCT/KR2018/003984 KR2018003984W WO2018186682A1 WO 2018186682 A1 WO2018186682 A1 WO 2018186682A1 KR 2018003984 W KR2018003984 W KR 2018003984W WO 2018186682 A1 WO2018186682 A1 WO 2018186682A1
Authority
WO
WIPO (PCT)
Prior art keywords
substrate
biosensor
norbornadiene
bio
compound
Prior art date
Application number
PCT/KR2018/003984
Other languages
English (en)
Korean (ko)
Inventor
권오석
이창수
박철순
김경호
김진영
박선주
이지연
Original Assignee
한국생명공학연구원
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 한국생명공학연구원 filed Critical 한국생명공학연구원
Publication of WO2018186682A1 publication Critical patent/WO2018186682A1/fr

Links

Images

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/544—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being organic
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54353—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals with ligand attached to the carrier via a chemical coupling agent

Definitions

  • the present invention relates to a biosensor substrate and a method of manufacturing the same used for manufacturing a multi-diagnosis biosensor.
  • biosensors eg PCR, diagnostic kits, etc.
  • biosensors eg PCR, diagnostic kits, etc.
  • It is widely used for industrial purposes.
  • the substrate (platform) of the biosensor is made of a material such as glass, silicon, or polymer, and the material of the substrate is determined according to the use of the biosensor.
  • biosensors such as real-time PCs (RT-PCR) and biochips are made of glass or silicon substrates.
  • biosensors The measurement of these biosensors is based on hydrodynamics, so the performance of the biosensors varies markedly with the surface properties of the substrate. For example, biochips have different flow rates depending on whether their surfaces are hydrophilic or hydrophobic, resulting in differences in reaction rates, which have a significant effect on the response time and sensitivity of the biosensor. Therefore, in order to improve the performance of the biosensor, it is important to consider what kind of surface treatment substrate is used.
  • an object of the present invention is to provide a biosensor substrate that can efficiently provide multiple diagnostic biosensors.
  • Another object of the present invention is to provide a method for manufacturing the biosensor substrate.
  • Another object of the present invention is to provide a biosensor comprising the biosensor substrate.
  • Another object of the present invention is to provide a method of manufacturing the biosensor.
  • the present invention to solve the above problems, the substrate portion; And a modification unit coupled to the surface of the substrate unit and including a light sensitive derivative, wherein the light sensitive derivative is a norbornadiene-based derivative.
  • the norbornadiene derivative may have a structure represented by Formula 1 below.
  • the substrate portion is made of glass, silicon and quartz It may include one or more selected from the group consisting of.
  • the modified part may be coupled to a plurality of surfaces of the substrate part.
  • the present invention a) preparing a substrate portion; b) reacting the prepared substrate portion with an amine-containing alkoxysilane-based compound; And c) introducing a norbornadiene compound to a substrate portion reacted with the amine-containing alkoxysilane compound to form a modified portion.
  • the amine-containing alkoxysilane compound is selected from the group consisting of (3-aminopropyl) trimethoxysilane and (3-aminopropyl) triethoxysilane ((3-Aminopropyl) triethoxysilane) It may be one or more.
  • the norbornadiene-based compound may be a compound represented by Formula 2 below.
  • the present invention the biosensor substrate; And a bio probe unit coupled to a reforming unit of the bio sensor substrate.
  • the bioprobe unit may include a plurality, and the plurality of bioprobes may probe different target materials from each other.
  • the present invention A) preparing the biosensor substrate; B) masking a portion of the biosensor substrate and irradiating light to form an activated reformed portion and an inactivated modified portion; C) binding a bioprobe to the activated reforming unit; D) activating the deactivated reformate; And E) combining the bioprobe unit that probes a different target material with the bioprobe unit coupled in step C) to the reformer activated in step D).
  • the activation of the reformed portion deactivated in step D) may be performed by heat treatment or reaction with a transition metal.
  • the biosensor substrate according to the present invention includes a reforming unit selectively activated or deactivated by light, the biosensor substrate may efficiently provide multiple diagnostic biosensors when the biosensor is manufactured using the biosensor substrate.
  • FIG 1 and 2 are reference diagrams for explaining the biosensor substrate of the present invention.
  • FIG. 3 is a reference diagram for explaining a method of manufacturing the biosensor substrate of the present invention.
  • FIG. 4 is a reference diagram for explaining the biosensor of the present invention.
  • FIG. 5 is a reference diagram for explaining a method of manufacturing a biosensor according to the present invention.
  • FIG. 6 is a reference diagram for explaining an experimental example 1 of the present invention.
  • FIG. 7 is a reference diagram for explaining an experimental example 2 of the present invention.
  • the surface of the biosensor substrate which is the base substrate of the biosensor
  • the conventional method for example, plasma treatment
  • the surface is selectively modified so that various bio Characterized in that it can be combined with the probe, it will be described in detail with reference to the drawings as follows.
  • the biosensor substrate of the present invention includes a substrate portion 10 and a reforming portion 20.
  • the substrate unit 10 included in the biosensor substrate of the present invention serves as a base of the biosensor substrate, and may be made of a material known in the art. Specifically, the substrate portion 10 may be made of one or more selected from the group consisting of glass, silicon, and quartz.
  • the reforming unit 20 included in the biosensor substrate of the present invention is present in combination with the surface of the substrate unit 10.
  • the reforming unit 20 is formed by undergoing a surface modification process of the substrate unit 10 in the manufacturing process of the biosensor substrate, and includes a light-sensitive derivative.
  • the light-sensitive derivative is a norbornadiene-based derivative, and may be a norbornadiene-based derivative including a norbornadiene group.
  • the modification unit 20 may be selectively activated or deactivated when light (for example, ultraviolet rays) is irradiated by the norbornadiene-based derivative.
  • the norbornadiene-based derivative is not particularly limited, but preferably has a structure represented by the following formula (1). This is because the compound represented by the following Chemical Formula 1 is excellent in reactivity with light and can easily induce activation and deactivation of the reforming unit 20 according to specific conditions.
  • the norbornadiene-based derivative may be coupled to the substrate portion 10 by a linker including an amine group on one side and a hydrophilic group on the other side. That is, the reforming unit 20 is composed of a linker and a norbonadiene-based derivative, the hydrophilic group of the linker is bonded to the surface of the substrate portion 10, the norbornadiene-based derivative is bonded to the amine group side of the linker is modified The portion 20 may be fixed to the surface of the substrate portion 10.
  • the linker may have a structure including a repeating unit represented by Formula 3 below.
  • n is an integer of 100 to 1,000,000.
  • * means a site where Formula 2 and Formula 3 are bonded to each other.
  • the modifying unit 20 may be coupled to the surface of the substrate unit 10 in plurality.
  • the present invention provides a method of manufacturing the above-described biosensor substrate, which will be described in detail with reference to FIG. 3 as follows.
  • substrate part 10 is prepared. Preparation of the substrate portion 10 may be made by a conventionally known method.
  • the prepared substrate portion 10 is reacted with an amine-containing alkoxysilane compound. Specifically, the substrate portion 10 is immersed in a solution in which an amine-containing alkoxysilane compound and an organic solvent (for example, benzene, toluene, xylene, etc.) are mixed and reacted for a predetermined time to the surface of the substrate portion 10.
  • an organic solvent for example, benzene, toluene, xylene, etc.
  • the amine-containing alkoxysilane-based compound is not particularly limited, (3-aminopropyl) trimethoxysilane and (3-aminopropyl) triethoxysilane ((3-Aminopropyl) triethoxysilane) It is preferably at least one compound selected from the group consisting of.
  • the reaction between the substrate portion 10 and the amine-containing alkoxysilane-based compound may be performed in the presence of an inert gas (eg, nitrogen or argon), and the reaction time may be 3 to 9 hours, although not particularly limited.
  • an inert gas eg, nitrogen or argon
  • a norbornadiene-based compound is introduced into the substrate 10 reacted with the amine-containing alkoxysilane-based compound to form a reformed portion 20.
  • the substrate portion 10 reacted with the amine-containing alkoxysilane-based compound is subjected to a norbornenadiene-based compound, which is a light-sensitive compound, and an organic solvent (for example, dimethylformamide, dimethylacetamide, tetrahydrofuran, N- Methyl-2-pyrrolidone and the like) are immersed in the mixed solution and reacted for a predetermined time to fix the derivative of the norbornadiene compound on the linker bonded to the surface of the substrate portion 10.
  • an organic solvent for example, dimethylformamide, dimethylacetamide, tetrahydrofuran, N- Methyl-2-pyrrolidone and the like
  • the norbornadiene-based compound may include a norbornadiene group.
  • the norbornadiene-based compound is not particularly limited, but is preferably a compound represented by the following formula (2).
  • the reaction time of the substrate unit 10 and the norbornadiene compound is not particularly limited, but may be 10 to 15 hours.
  • the present invention provides a biosensor capable of probing (detecting) various bio target materials, which will be described in detail with reference to FIG. 4.
  • the biosensor of the present invention includes a biosensor substrate 100 and a bioprobe unit 200.
  • the biosensor substrate 100 included in the biosensor of the present invention serves as a base substrate of the biosensor. It is the same as that described in "Bio-Sensor Substrate” and will be omitted.
  • the bioprobe 200 included in the biosensor of the present invention is to be coupled to the modified portion 20 of the biosensor substrate 100 (specifically, to the norbornadiene derivative of the modified portion 20), Probe and detect bio targets (eg, target nucleic acids, blood glucose, glycated proteins, etc.).
  • the bioprobe 200 may be a functional group (eg, -S-, etc.) coupled with the reforming unit 20 of the biosensor substrate 100 and a reactor (eg, an antigen) capable of binding to a biotarget material. , Aptamer, protein, etc.) is not particularly limited.
  • a plurality of bioprobes 200 included in the biosensor may also be provided.
  • the plurality of bio probes may probe different bio target materials from each other, and accordingly, the present invention may provide a multi-diagnosis bio sensor.
  • the present invention provides a method of manufacturing the above-described biosensor, which will be described in detail with reference to FIG. 5 as follows.
  • the biosensor substrate 100 described above is prepared.
  • a portion of the prepared biosensor substrate 100 is masked and irradiated with light (for example, ultraviolet rays) to form the activated reformed portion 20a and the inactivated modified portion 20b.
  • light for example, ultraviolet rays
  • the mask is placed on the selected region and irradiated with light, and the modified portion 20a of the selected region is maintained in an active state.
  • the reformed portion 20b of the non-selected region may be placed in an inactive state by the reaction of the light-sensitive derivative with light to form the activated reformed portion 20a and the inactivated modified portion 20b.
  • the bio probe 200a is coupled to the activated reforming unit 20a.
  • the bio probe 200a may be combined by a conventionally known method (for example, bio-thiolation).
  • the deactivated reforming unit 20b is activated.
  • the method of activating the deactivated reforming unit 20b is not particularly limited, but may be performed by heat treatment or reaction with a transition metal.
  • the heat treatment condition of the reforming unit 20b is not particularly limited, but may be performed at 70 to 90 ° C. for 15 to 24 hours.
  • the biosensor substrate 100 is immersed in a solution containing silver (Ag), cobalt (Co), or tin (Sn) and reacted for 10 to 14 hours. It can be made to.
  • the bio-probe 200b for probing different bio target materials from the bio-probe 200a coupled in the step C) is coupled to the reformed portion 20b activated through the step D).
  • the reforming unit 20a to which the bio probe unit 200a is coupled, and a reforming unit to couple the new bio probe unit 200b to probe a different bio target material from the bio probe unit 200a to which the bio probe unit 200a is coupled ( 20b) masking the region and irradiating light (for example, ultraviolet rays) to deactivate the unmasked region, and then combining the new bioprobe 200b to process the bio-bonded in step C).
  • the bio probe 200b may be combined with the probe 200a to probe different bio target materials.
  • Combination of the bioprobe 200a and the bioprobe 200b for probing a different bio target material may be generally performed by a known method (eg, bio-thiolation).
  • the present invention can produce a biosensor capable of probing and detecting various bio target materials.
  • a biosensor substrate and a biosensor using a light sensitive compound capable of reversible reaction which is inactivated when irradiated with light and activated by specific conditions (eg, heat treatment, reaction with a transition metal, etc.) Since the manufacturing of the biosensor to include can increase the manufacturing efficiency of the multi-diagnostic biosensor.
  • the biosensor substrate in irradiating light to a biosensor substrate including a modified portion combined with a light-sensitive derivative, the biosensor substrate is selectively activated or deactivated by irradiating light for each desired region.
  • the biosensor is manufactured by combining various bioprobes in the region, multiple diagnostic biosensors may be efficiently manufactured.
  • the present invention can easily induce the activation and deactivation of the biosensor substrate (region-by-region) using a mask, it is possible to easily manufacture a biosensor having a micro-miniature pattern.
  • Dicyclopentadiene, 2-butynedioic acid, 1,4-dioxane, N, N'-dicyclohexylcarbodiimide (N, N ') -Dicyclohexylcarbodiimide) and 3- (aminopropyl) trimethoxysilane (97%) were each purchased from Aldrich and used without purification.
  • a JEOL 3700 was used as an instrument.
  • Dicyclopentadiene (7 mL, 52 mmol) was distilled off to obtain monomeric cyclopentadiene (monomeric cyclopentadiene).
  • the resulting monomeric cyclopentadiene (2.0 g, 30 mmol) was mixed with 1,4-dionic acid (20 mL) and 2-butyndioic acid (3.0 g, 26.3 mmol) under an ice-water bath. To the solution. Next, the solution containing monomeric cyclopentadiene was stirred at room temperature overnight, and then hexane (5 mL) was added and collected to obtain a solid precipitate (4.18 g, yield: 88%).
  • the glass substrate was immersed in a solution of toluene (20 ml) and 3- (aminopropyl) trimethoxysilane (10 ⁇ l), and shaken for 6 hours in a nitrogen atmosphere to give amine-functionalized glass. A substrate was obtained. Next, the glass substrate functionalized with amine was washed with toluene, and unreacted 3- (aminopropyl) trimethoxysilane was removed with a mixture of toluene and methanol in a volume ratio of 1: 1.
  • 2.5-norbornadiene-2,3-dicarboxylic anhydride obtained in Synthesis Example 2 was dissolved in dimethylformamide. Next, the amine-functionalized glass substrate is immersed in a solution in which 2.5-norbornadiene-2,3-dicarboxylic acid anhydride is dissolved, and shaken for 12 hours to give 2.5-norbornadiene- to the amine-functionalized glass substrate. 2,3-dicarboxylic anhydride was introduced.
  • the glass substrate reacted with the thiol-terminal bioprobe (thiol-terminal bioprobe) was immersed in the mixed solution of AgClO 4 and methanol and reacted for 12 hours to activate the surface of the glass substrate that was inactivated (step D).
  • steps A) to D) were repeated to prepare another thiol-terminal bioprobe (thiol-terminal bioprobe) (Aptamer (HS-TATCAGTTCTTTGACCTTTGTCA-FAM-3 ', Bioneer)).
  • a glass substrate having an activation region and an inactivation region was immersed in a solution in which 4-bromobenzenethiol (5 mg) was dissolved in dimethylformamide (1 mL) and reacted at room temperature for 12 hours.
  • the glass substrate was washed with dimethylformamide and deionized water.
  • the cleaned glass substrate was analyzed by x-ray photoelectron spectroscopy, and the results are shown in FIG. 6.
  • the biosensor prepared in Preparation Example 1 was analyzed by a fluorescence microscope (EVOS® FL Cell Imaging System, ThermoFisher SCIENTIFIC). Is shown in FIG. 7.

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

L'invention concerne un substrat de biocapteur, son procédé de production, et un biocapteur le comprenant.
PCT/KR2018/003984 2017-04-04 2018-04-04 Substrat de biocapteur, son procédé de production, et biocapteur le comprenant WO2018186682A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2017-0043730 2017-04-04
KR1020170043730A KR101875470B1 (ko) 2017-04-04 2017-04-04 바이오 센서 기판, 이의 제조방법 및 이를 포함하는 바이오 센서

Publications (1)

Publication Number Publication Date
WO2018186682A1 true WO2018186682A1 (fr) 2018-10-11

Family

ID=62920974

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2018/003984 WO2018186682A1 (fr) 2017-04-04 2018-04-04 Substrat de biocapteur, son procédé de production, et biocapteur le comprenant

Country Status (2)

Country Link
KR (1) KR101875470B1 (fr)
WO (1) WO2018186682A1 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101875471B1 (ko) * 2017-04-04 2018-07-06 한국생명공학연구원 바이오 센서 기판, 이의 제조방법 및 이를 포함하는 바이오 센서

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20050014409A (ko) * 2003-07-31 2005-02-07 삼성에스디아이 주식회사 생체물질 고정용 기판 및 이의 제조방법
US20080161200A1 (en) * 2006-12-05 2008-07-03 The Board Of Trustees Of The Leland Stanford Junior University Biomolecule Immobilization on Biosensors
KR20090119476A (ko) * 2008-05-16 2009-11-19 한국전자통신연구원 바이오센서의 기판의 패턴의 제조 방법 및 이를 이용한바이오센서
US20130261211A1 (en) * 2010-10-19 2013-10-03 Max-Planck-Gesellschaft Zur Foerderung Der Wissenschaften E.V. Process for the modification of polymers, in particular polymer nanoparticles
KR20150123391A (ko) * 2014-04-24 2015-11-04 한국과학기술원 바이오물질 부착을 위한 기질필름의 표면처리방법 및 이를 이용한 면역센서칩

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH05311579A (ja) * 1992-05-01 1993-11-22 Hiroshi Kiyokawa 太陽光吸収蓄熱繊維素材とその製造法

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20050014409A (ko) * 2003-07-31 2005-02-07 삼성에스디아이 주식회사 생체물질 고정용 기판 및 이의 제조방법
US20080161200A1 (en) * 2006-12-05 2008-07-03 The Board Of Trustees Of The Leland Stanford Junior University Biomolecule Immobilization on Biosensors
KR20090119476A (ko) * 2008-05-16 2009-11-19 한국전자통신연구원 바이오센서의 기판의 패턴의 제조 방법 및 이를 이용한바이오센서
US20130261211A1 (en) * 2010-10-19 2013-10-03 Max-Planck-Gesellschaft Zur Foerderung Der Wissenschaften E.V. Process for the modification of polymers, in particular polymer nanoparticles
KR20150123391A (ko) * 2014-04-24 2015-11-04 한국과학기술원 바이오물질 부착을 위한 기질필름의 표면처리방법 및 이를 이용한 면역센서칩

Also Published As

Publication number Publication date
KR101875470B1 (ko) 2018-07-06

Similar Documents

Publication Publication Date Title
EP1144677B1 (fr) Immobilisation de molecules sur des surfaces par des brosses polymeres
WO2009136741A1 (fr) Nouveau composite cœur-coque or-argent convenant comme biocapteur
CA1338597C (fr) Reactifs pour la preparation d'oligonucleotides marques en 5'
WO2018186683A1 (fr) Substrat de biocapteur, son procédé de production, et biocapteur le comprenant
WO2019203493A1 (fr) Capteur biologique à base d'électrode à puits multiples
WO2013176458A1 (fr) Biocapteur optique
CN108424844B (zh) 一种单分子测序芯片及其制备方法
WO2018186682A1 (fr) Substrat de biocapteur, son procédé de production, et biocapteur le comprenant
WO2020226232A1 (fr) Dispositif de transistor électrochimique organique et son procédé de fabrication
WO2013141465A1 (fr) Sonde fluorescente à deux photons de couleur variable permettant de détecter l'activité du peroxyde dans des mitochondries, procédé de production de ladite sonde et procédé d'imagerie de l'activité du peroxyde dans des mitochondries utilisant ladite sonde
JP3760158B2 (ja) 新規の伝導性高分子、これを利用したセンサー及び標的物質検出方法
WO2020013668A1 (fr) Substrat pour amplification d'acide nucléique, et son procédé de fabrication
EP1035218A1 (fr) Immobilisation des molécules sur des surfaces via des brosses de polymer
CN112694394A (zh) 超灵敏多输出信号生物传感器及其制备方法和应用
WO2019059663A1 (fr) Particules composites à nano-espaces et leur procédé de préparation
WO2019050222A1 (fr) Procédé permettant d'isoler et de purifier de façon simple et efficace des oligonucléotides marqués chimiquement au moyen d'une régulation de ph
WO2012081856A2 (fr) Procédé de préparation d'une bande de support solide poreux revêtu d'une sonde d'acide nucléique
WO2018230803A1 (fr) Procédé de préparation d'un nanocomposite de diffusion raman exaltée de surface pour détecter des agents pathogènes, et procédé de détection d'agents pathogènes en utilisant celui-ci et la pcr numérique
CN111961076B (zh) 一种具有分子内电荷转移特征的1,4-二甲基喹啉衍生物及其制备方法与应用
WO2012102584A9 (fr) Capteur pour détecter un virus cible, procédé pour cribler un virus cible et analyser le site de mutation avec celui-ci
KR100381457B1 (ko) 이중가닥 디엔에이의 전기화학적 검출에 유용한인터칼레이터 화합물, 이의 제조 방법 및 이 방법에유용한 중간체
WO2020013664A1 (fr) Substrat pour l'amplification d'acide nucléique, et son procédé de fabrication
WO2014035039A1 (fr) Méthode d'analyse de transcription génétique à l'aide d'un capteur de résonance plasmonique de surface
KR20080089834A (ko) ＰＣＲ 생성물의 ｄｓ-ＤＮＡ 검출용 화합물, ＰＣＲ생성물의 ｄｓ-ＤＮＡ 검출용 칩, 및 그 칩을 이용하여ＰＣＲ 생성물의 ｄｓ-ＤＮＡ를 검출하는 방법
US7833406B2 (en) Gene detection method, and intercalator

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 18781462

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 18781462

Country of ref document: EP

Kind code of ref document: A1

点击 这是indexloc提供的php浏览器服务，不要输入任何密码和下载