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WO2018171010A1 - Dispositif de culture de champignons de type à couvercle supérieur - Google Patents

Dispositif de culture de champignons de type à couvercle supérieur Download PDF

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Publication number
WO2018171010A1
WO2018171010A1 PCT/CN2017/083399 CN2017083399W WO2018171010A1 WO 2018171010 A1 WO2018171010 A1 WO 2018171010A1 CN 2017083399 W CN2017083399 W CN 2017083399W WO 2018171010 A1 WO2018171010 A1 WO 2018171010A1
Authority
WO
WIPO (PCT)
Prior art keywords
culture
culture dish
overcapped
fungus
slide
Prior art date
Application number
PCT/CN2017/083399
Other languages
English (en)
Chinese (zh)
Inventor
于津
Original Assignee
珠海美华医疗科技有限公司
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 珠海美华医疗科技有限公司 filed Critical 珠海美华医疗科技有限公司
Publication of WO2018171010A1 publication Critical patent/WO2018171010A1/fr

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Definitions

  • the present invention relates to a microbial experimental device, and more particularly to a supercapped fungal culture device.
  • the present invention provides a capped fungal culture device which can observe the growth of fungal culture under an oil microscope and a high power microscope, and reduces the use cost.
  • a capped fungus culture device comprising a slide glass (1) and a culture dish (2), characterized in that the slide glass (1) is disposed at Below the culture dish (2), the slide (1) and the culture dish (2) are integrally formed and form a fungal culture chamber (3), wherein the slide (1) serves as a chassis for the culture dish (2).
  • the upper portion of the culture chamber (3) is provided with an upper cover (5), the upper cover (5) is a glass piece, and the side wall of the culture dish is provided with a through hole (4) for extending into the inoculation, The through hole (4) is in communication with the fungal culture chamber (3).
  • the axial direction of the through hole is perpendicular to the longitudinal direction of the slide glass.
  • the petri dish has a circular cross section.
  • the cover glass has a circular shape and a diameter larger or smaller than the diameter of the culture dish.
  • the cover glass is ultrasonically welded to the upper portion of the culture dish.
  • the slide glass is provided with a frosted area on both sides.
  • the slide glass and the culture dish are each made of a light transmissive polystyrene material.
  • the slide has a length of 50 to 100 mm and a width of 20 to 30 mm.
  • the four corners of the slide have a chamfer.
  • the slide has a length of 50 to 100 mm and a width of 20 to 30 mm.
  • the culture dish has a diameter of 15 to 25 mm and a height of 2 to 5 mm, and the through hole has an inner diameter of 1.0 to 4.0 mm.
  • one side of the slide glass is further provided with a trademark area marked with a trademark.
  • the present invention provides an overcapped fungal culture apparatus which utilizes a glass sheet or other material having a thinner thickness by modifying the upper lid of the culture dish.
  • the microscope is made thinner, so that the microscope is closer to the fungus for observation, so that the morphology of the fungus can be observed more clearly under the same multiple microscope, thereby achieving the use of a low power microscope.
  • the cost of purchasing a high power microscope is reduced, and significant economic benefits are obtained.
  • Figure 1 is a schematic structural view of an embodiment of the present invention
  • Figure 2 is a side plan view of an embodiment of the present invention.
  • Figure 3 is a rear elevational view of Figure 2, in accordance with one embodiment of the present invention.
  • Figure 4 is a rear elevational view of Figure 2 of another embodiment of the present invention.
  • the top cover fungus culture apparatus comprises a slide glass 1 on the front side of which a culture dish 2 is disposed, the slide glass 1 and the culture dish 2 are integrally formed, and the slide glass 1 is located under the culture dish 2 As its chassis, both the slide 1 and the petri dish 2 are made of a light transmissive polystyrene material.
  • the side wall of the culture dish 2 is provided with a through hole 4 for extending into the inoculation, wherein the axial direction of the through hole 4 is perpendicular to the longitudinal direction of the slide glass 1, and the through hole 4 is used for inoculating the needle to advance the fungus into the needle. Inoculation in a solid medium, the substance in the fungal culture chamber 3 shown in Fig. 2 is not volatile due to the small pore size.
  • the overcapped fungal culture apparatus of the present invention further includes an upper cover 5 made of glass and ultrasonically welded to the top end of the side wall of the culture dish 2.
  • the upper cover 5 can be replaced with a cover glass, and the side wall of the culture dish 2 can be sealed with paraffin wax to meet different requirements, and is also closer to the conventional microscopic observation mode.
  • the integrally formed slide 1 and the culture dish 2 form a fungal culture chamber 3, the through hole 4 is in communication with the fungal culture chamber 3, and both sides of the slide 1 are provided with a frosted area 7 for easy marking. Further, one side of the slide glass 1 is provided with a trademark area 8 for marking a trademark, the size and position of which are determined as needed.
  • the outer diameter of the upper cover 5 is smaller than the outer diameter of the culture dish 2 and larger than the inner diameter of the culture dish 2 to ultrasonically weld the upper cover 5 to the culture dish 2.
  • the weld bead is located on the upper annulus of the culture dish 2, and the joint 6 of the culture dish 2 and the upper cover 5 is sealed with paraffin.
  • the outer diameter of the upper cover 5 is larger than the outer diameter of the culture dish 2, so that the upper cover 5 is ultrasonically welded to the upper ring surface of the culture dish 2, and is welded.
  • the slit is located at the joint 6 of the outer edge of the torus of the petri dish 2 and the upper cover 5.
  • the cross section of the culture dish 2 may be a circular or elliptical shape or a regular polygon.
  • the culture dish 2 is circular in cross section
  • the slide glass 1 is rectangular, wherein the length of the slide 1 is It is 50 ⁇ 100mm, the width is 20 ⁇ 30mm, the diameter of the culture dish 2 is 15 ⁇ 25mm, the height is 2 ⁇ 5mm, the inner diameter of the through hole is 1.0 ⁇ 4.0mm, specifically, in this embodiment, the slide 1
  • the length of the dish is 75.9mm, the width is 25.2mm, the diameter of the culture dish 2 is 23.1mm, the height is 4.35mm, and the inner diameter of the through hole 4 is 2.7mm, so that the culture device can be placed under the microscope, and no additional fungus is needed. Place under the microscope to prevent laboratory contamination.
  • the present invention provides an overcapped fungal culture apparatus which utilizes a glass sheet or other material having a thinner thickness by modifying the upper lid of the culture dish.
  • the microscope is made thinner, so that the microscope is closer to the fungus for observation, so that the morphology of the fungus can be observed more clearly under the same multiple microscope, thereby achieving the use of a low power microscope.
  • the cost of purchasing a high power microscope is reduced, and significant economic benefits are obtained.

Landscapes

  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

La présente invention concerne un dispositif de culture de champignons de type à couvercle supérieur, comprenant une lame de verre (1) et une boîte de culture (2), la lame de verre (1) étant disposée au-dessous de la boîte de culture (2) ; la lame de verre (1) et la boîte de culture (2) sont d'une structure intégrée et forment une chambre de culture de champignons (3) ; la lame de verre (1) sert de plateau inférieur de la boîte de culture (2) ; un couvercle supérieur (5) est disposé au niveau de la partie supérieure de la chambre de culture (3) ; le couvercle supérieur (5) est une feuille de verre ; la paroi latérale de la boîte de culture est pourvue d'un trou traversant (4) permettant la pénétration et l'inoculation ; et le trou traversant (4) est en communication avec la chambre de culture de champignons (3).
PCT/CN2017/083399 2017-03-22 2017-05-08 Dispositif de culture de champignons de type à couvercle supérieur WO2018171010A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN201720284583 2017-03-22
CN201720284583.4 2017-03-22

Publications (1)

Publication Number Publication Date
WO2018171010A1 true WO2018171010A1 (fr) 2018-09-27

Family

ID=63584934

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CN2017/083399 WO2018171010A1 (fr) 2017-03-22 2017-05-08 Dispositif de culture de champignons de type à couvercle supérieur

Country Status (1)

Country Link
WO (1) WO2018171010A1 (fr)

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN201427969Y (zh) * 2009-06-23 2010-03-24 王金柱 新型细胞培养玻片
CN201737930U (zh) * 2010-07-15 2011-02-09 中国人民解放军第四军医大学 一种真菌小培养装置
CN201915102U (zh) * 2011-01-26 2011-08-03 于莉 细胞培养玻片
CN203319999U (zh) * 2013-06-26 2013-12-04 漯河医学高等专科学校 一种用于真菌生长形态显微观察的玻片培养器皿
CN205099668U (zh) * 2015-10-30 2016-03-23 陈鹏 一种用于观察真菌形态的培养装置
CN106399092A (zh) * 2016-10-14 2017-02-15 珠海美华医疗科技有限公司 真菌小培养装置

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN201427969Y (zh) * 2009-06-23 2010-03-24 王金柱 新型细胞培养玻片
CN201737930U (zh) * 2010-07-15 2011-02-09 中国人民解放军第四军医大学 一种真菌小培养装置
CN201915102U (zh) * 2011-01-26 2011-08-03 于莉 细胞培养玻片
CN203319999U (zh) * 2013-06-26 2013-12-04 漯河医学高等专科学校 一种用于真菌生长形态显微观察的玻片培养器皿
CN205099668U (zh) * 2015-10-30 2016-03-23 陈鹏 一种用于观察真菌形态的培养装置
CN106399092A (zh) * 2016-10-14 2017-02-15 珠海美华医疗科技有限公司 真菌小培养装置

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