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WO2018164567A1 - Système de manipulation d'échantillons biologiques à haute viscosité - Google Patents

Système de manipulation d'échantillons biologiques à haute viscosité Download PDF

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Publication number
WO2018164567A1
WO2018164567A1 PCT/MX2018/050007 MX2018050007W WO2018164567A1 WO 2018164567 A1 WO2018164567 A1 WO 2018164567A1 MX 2018050007 W MX2018050007 W MX 2018050007W WO 2018164567 A1 WO2018164567 A1 WO 2018164567A1
Authority
WO
WIPO (PCT)
Prior art keywords
biological samples
samples
sampling device
handling biological
samples according
Prior art date
Application number
PCT/MX2018/050007
Other languages
English (en)
Spanish (es)
Inventor
Carmen Guadalupe PANIAGUA CHÁVEZ
Karina MORALES UENO
Original Assignee
Centro De Investigación Científica Y De Educación Superior De Ensenada, Baja California (Cicese)
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Centro De Investigación Científica Y De Educación Superior De Ensenada, Baja California (Cicese) filed Critical Centro De Investigación Científica Y De Educación Superior De Ensenada, Baja California (Cicese)
Publication of WO2018164567A1 publication Critical patent/WO2018164567A1/fr

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Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/10—Preservation of living parts
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/02—Devices for withdrawing samples

Definitions

  • the present invention relates to a system for handling biological samples and more particularly to a system for handling biological samples of very high viscosity, which allows sampling of very thick biological fluids and / or tissues, which cannot be handled with existing conventional sampling devices.
  • the present invention relates to a novel sampling device that allows the fluids and / or tissues collected to be submerged in working solutions without them falling out of the device, while also allowing the correct treatment of the samples.
  • the collection and handling of samples of fluids and / or biological tissues, whether for study and / or storage, requires specialized devices that facilitate the work of collecting and moving the collected samples.
  • Said devices are designed to contain the biological samples in one or more cavities and also allow the safe transfer of the specimens during the treatments to which they are subjected during the conservation and / or analysis processes.
  • One of the types of sampling that entails major inconveniences during its treatment and transfer, is the sampling of very thick biological material, since being in a state semi-solid, they require specific devices to carry out the taking of them.
  • cryopreservation the type of sampling device is very important, since it must allow the sample to be effectively contained and ensure that the sample can be contacted with cryoprotective and freezing solutions, as well as withstand sudden changes in temperature. Without suffering mechanical wear.
  • cryoloop which is composed of a ring with a stem that can be attached to a vial stopper, if necessary;
  • the cryoloop can be rigid (made of metal, aluminum, steel etc. etc.) or flexible (ring made of nylon).
  • other types of devices can be used such as, for example, pipette tips (apparatus for obtaining and measuring volumes of a solution or reagent) and grids for electron microscopy samples.
  • Said instrument is made of a material resistant to submersion in liquid nitrogen.
  • the device described in the aforementioned application is made up of a tubular protector within which a storage member is stored that has a recess in its end portion where the tissue sample is contained.
  • said device does not allow the submersion of the sample in cryopreservation fluids directly and also due to its configuration makes it impossible to load samples of high viscosity fluids, due to the reduced effective area of suction.
  • Patent application EP 2353383 A2 describes a device for the vitrification of samples that is characterized by allowing a rapid closure thereof in order to avoid contamination.
  • Said device comprises a reservoir into which a microcapillary is introduced, which is responsible for the collection and preservation of the sample.
  • the device described when using microcapillaries does not allow mixing the sample with working solutions and much less loading highly viscous fluids.
  • patent 102669088 describes a device for the vitrification of samples, which is formed by a storage tube with a counterweight at its sealed end, said device allows the insertion of capillaries with biological samples of reproductive cells and embryos.
  • the use of capillaries limits the type of samples that can be taken with said device; It also has the same disadvantage of not allowing direct interaction of the sample with the work solutions.
  • Patent Application CN 103202285 describes a device for the vitrification of samples comprising a sheet-shaped loading member that is placed at the closed end of a protective jacket. Said device allows not only liquid samples to be taken, but also tissue fragments; however, since it does not have surfaces that delimit a single containment chamber, Samples taken can be dropped during treatment operations with working fluids.
  • Patent application WO 2014191501 Al describes a device for vitrification of biological samples, consisting of an internal tube sealed at both ends containing the sample and which is in turn inserted into a second outer tube that has an element of weight to facilitate its placement inside liquid nitrogen;
  • the arrangement described in the aforementioned patent application in no way allows the sample to be contacted in a controlled manner with cryoprotective agents, much less the load of high viscosity fluids due to the small diameter of the collecting part.
  • US patent application 20040259072 Al describes a device for the cryopreservation of ovules, which consists of a suction tip that is placed inside a protective jacket. Said device works with liquid samples and is unable to work with viscous samples. Likewise, there is no way for the described device to allow specimen contact with working mixtures.
  • Patent application US20140157798 Al describes a device for the cryopreservation and / or vitrification of biological samples in a freezing medium, said device comprising a bath to receive the samples, which has an overflow chamber to maintain the height of the liquid at a same level even when Multiple samples are immersed in it.
  • cryopreservation liquid is shared among all samples, so it is necessary to provide each of them with an individual cover to avoid cross contamination.
  • the way to place the cryopreservation vials inside the bathroom requires their passage through a perforated surface, which could cause contamination by involuntary contacts.
  • the vials are covered by a structure similar to a sheath that must be cut for handling, a step that if carried out in a very careful way could damage the structure of the sample handling device.
  • patent application JP2007111000 describes a tool for vitrification of mammalian germ cells, said tool comprises a freezing vessel with a small diameter portion that serves to aspirate the sample.
  • Said small diameter portion is covered by a protection member and the freezing container has a sealing element that seals its rear portion.
  • This tool is attached to the free end of a suction device, so the sample to be collected and treated must be fluid enough to be loaded into the tool due to its construction. Additionally, since the sample has to be aspirated, it cannot be viscous since it would completely clog the inlet opening thereby preventing its aspiration. The foregoing considerably limits the use of the tool, since in most situations it is necessary Dilute the original sample to allow its correct treatment, which completely breaks its original microstructure, which leads to a series of important inconveniences in the case of treating samples that depend on its original viscosity to maintain its viability.
  • sampling devices cannot handle samples with a very high viscosity degree, since they are designed primarily for the aspiration of low-density fluids such as blood, saliva and / or seminal mammalian fluid, so that work with samples of eg crustacean spermatophore, mollusc spermatophore and / or viscous consistency samples is disabled with the currently available devices.
  • the present invention aims to provide a high viscosity sample handling system that has an open sampling device that facilitates the preparation and Sample processing and also allow them to be immersed in work solutions.
  • a particular objective of the present invention is to provide a system that allows preparing, processing and freezing multiple high viscosity samples with a support frame for the placement of multiple devices of Sampling on a work fluid containment surface, in order to work with multiple samples at the same time, without mixing them during processing.
  • Another objective of the present invention is to provide a high viscosity sample handling system that has means in the sampling device that allows working fluids to come into contact with the largest possible surface area of biological samples.
  • a further objective of the present invention is to provide a high viscosity sample management system that supports the operating conditions of most existing cryopreservation protocols.
  • Figure 1 shows a perspective view of the system for handling high viscosity biological samples of the present invention.
  • Figure 2 shows a perspective view of the sampling device (A).
  • Figure 3 shows a perspective view of the support (B).
  • Figure 4 shows a perspective view of the multiwell plate (C).
  • the present invention relates to a system for handling high viscosity biological samples with means for controlled submersion thereof in working solutions, such as those used for example in cryopreservation processes.
  • the system for handling biological samples of the present invention is composed of a sampling device (A); a support (B) and; a multiwell plate (C). These elements will be described in more detail below.
  • the sampling device (A) is constituted by an elongated surface (1), substantially rectangular in shape, which at one of its ends has a "U" shaped surface (2) formed by three faces perpendicular to said elongated surface (1); while, on the other end, a threaded cover (3) is attached that allows the sampling device (A) to be closed on conventional sample containers and a piece of magnetic metal (4).
  • Said "U" shaped surface (2) is delimiting a receptacle (5) that serves for the collection and retention of high viscosity biological samples.
  • the internal walls of the surface in the form of "U" (2) have channels or grooves, which facilitate the adhesion of the sample, as well as the penetration of the work solutions.
  • the support (B) comprises a central stringer (6), which has a magnetic strip (7) attached along its entire surface and; a pair of inverted "V" telescopic legs (8), which can be individually adjusted for placing said support on irregular surfaces, for example in figure 3 one of the pairs of "telescopic legs” is shown. V "inverted (8) in the extended position, while the other pair is in the retracted position, with a difference in height (h) between them.
  • Said support is designed to allow placing at least one sampling device (A) using the attraction between the magnetic metal part (4) and the magnetic strip (7).
  • the multiwell plate (C) is constituted by a base (9) in which two rows of wells (10) are placed in a substantially circular way, separated from each other in an equidistant manner, said wells having a height substantially greater than the total length of the area bounded by the "U" shaped surface (2) of the sampling device (A), such that at Place working solutions, such as those used during cryopreservation processes, the height of the fluid contained in the wells exceeds the height of the treated sample.
  • the system of the present invention becomes especially useful in the reproductive management of biological species such as invertebrates, in which sperm samples have a high degree of viscosity and cannot be handled with the devices available in the market.
  • both the sampling device (A) and the support (B) and the multiwell plate (C) are made of a material resistant to sudden changes in temperature, such as for example food grade plastic (Nylon 66), aluminum, high density polyethylene and / or Vitron®; Therefore, they can be used in vitrification protocols for biological samples even at temperatures below -80 ° C.
  • a material resistant to sudden changes in temperature such as for example food grade plastic (Nylon 66), aluminum, high density polyethylene and / or Vitron®; Therefore, they can be used in vitrification protocols for biological samples even at temperatures below -80 ° C.
  • the sampling device (A) has a pair of breakable arms (r) coupled on the elongated surface (1), close to the portion where the screw cap (3) is located, such that it is possible to place said device in the mouth of conventional vials without it falling inside if necessary, being possible to detach said breakable arms (r), to store the samples in threaded vials.

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Engineering & Computer Science (AREA)
  • Dentistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

L'invention concerne un système pour la manipulation d'échantillons biologiques à haute viscosité constitué d'un dispositif de prélèvement d'échantillons ouvert, qui permet la charge de matériaux biologiques hautement visqueux ; un support magnétique pour la fixation d'au moins un dispositif de prélèvement d'échantillons; et une plaque multipuits pour l'immersion des tissus et/ou fluides contenus dans le dispositif de prélèvement d'échantillons, sans nécessité de mise en oeuvre d'opérations de transvasement.
PCT/MX2018/050007 2017-03-06 2018-03-01 Système de manipulation d'échantillons biologiques à haute viscosité WO2018164567A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
MX2017004174A MX369254B (es) 2017-03-06 2017-03-06 Sistema de manejo de muestras biológicas de alta viscosidad.
MXMX/A/2017/004174 2017-03-06

Publications (1)

Publication Number Publication Date
WO2018164567A1 true WO2018164567A1 (fr) 2018-09-13

Family

ID=63447907

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/MX2018/050007 WO2018164567A1 (fr) 2017-03-06 2018-03-01 Système de manipulation d'échantillons biologiques à haute viscosité

Country Status (2)

Country Link
MX (1) MX369254B (fr)
WO (1) WO2018164567A1 (fr)

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0187684A2 (fr) * 1980-09-24 1986-07-16 The Regents Of The University Of Minnesota Dispositif collecteur d'échantillons
US20060154232A1 (en) * 2003-07-16 2006-07-13 Fraunhofer Gesellschaft Zur Forderung Der Angewandten Forschung E.V. Stack of supports, in particular for cryopreservation of biological samples
EP1939604A1 (fr) * 2006-12-29 2008-07-02 Preanalytix GmbH Dispositif de collecte pour le déblocage sélectif d'un échantillon biologique
US20130052730A1 (en) * 2011-08-26 2013-02-28 Biocision, Llc Methods and system for cryogenic preservation of cells
CN202819433U (zh) * 2012-10-30 2013-03-27 山东省齐鲁干细胞工程有限公司 一种精子冷冻保存装置

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0187684A2 (fr) * 1980-09-24 1986-07-16 The Regents Of The University Of Minnesota Dispositif collecteur d'échantillons
US20060154232A1 (en) * 2003-07-16 2006-07-13 Fraunhofer Gesellschaft Zur Forderung Der Angewandten Forschung E.V. Stack of supports, in particular for cryopreservation of biological samples
EP1939604A1 (fr) * 2006-12-29 2008-07-02 Preanalytix GmbH Dispositif de collecte pour le déblocage sélectif d'un échantillon biologique
US20130052730A1 (en) * 2011-08-26 2013-02-28 Biocision, Llc Methods and system for cryogenic preservation of cells
CN202819433U (zh) * 2012-10-30 2013-03-27 山东省齐鲁干细胞工程有限公司 一种精子冷冻保存装置

Also Published As

Publication number Publication date
MX2017004174A (es) 2018-09-05
MX369254B (es) 2019-09-24

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