WO2016117767A1 - Tubercular bacillus slide staining apparatus - Google Patents
Tubercular bacillus slide staining apparatus Download PDFInfo
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- WO2016117767A1 WO2016117767A1 PCT/KR2015/004772 KR2015004772W WO2016117767A1 WO 2016117767 A1 WO2016117767 A1 WO 2016117767A1 KR 2015004772 W KR2015004772 W KR 2015004772W WO 2016117767 A1 WO2016117767 A1 WO 2016117767A1
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- Prior art keywords
- dyeing
- slide
- plate
- rotary
- unit
- Prior art date
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- 238000010186 staining Methods 0.000 title claims abstract description 34
- 241000193830 Bacillus <bacterium> Species 0.000 title abstract description 4
- 238000005406 washing Methods 0.000 claims abstract description 25
- 239000007788 liquid Substances 0.000 claims abstract description 13
- 238000004043 dyeing Methods 0.000 claims description 101
- 241000187479 Mycobacterium tuberculosis Species 0.000 claims description 14
- 238000000034 method Methods 0.000 claims description 8
- 238000005507 spraying Methods 0.000 claims description 5
- 238000003825 pressing Methods 0.000 claims description 4
- 238000003475 lamination Methods 0.000 claims description 3
- 230000008878 coupling Effects 0.000 claims description 2
- 238000010168 coupling process Methods 0.000 claims description 2
- 238000005859 coupling reaction Methods 0.000 claims description 2
- 201000008827 tuberculosis Diseases 0.000 claims description 2
- 230000003749 cleanliness Effects 0.000 abstract description 3
- 238000012423 maintenance Methods 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 17
- 238000010438 heat treatment Methods 0.000 description 12
- 238000004140 cleaning Methods 0.000 description 3
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 239000012192 staining solution Substances 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 238000009969 top dyeing Methods 0.000 description 1
- 238000010792 warming Methods 0.000 description 1
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/29—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands using visual detection
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
Definitions
- the present invention relates to a Mycobacterium tuberculosis slide dyeing apparatus, and more particularly, to a Mycobacterium tuberculosis slide dyeing apparatus, comprising a hot air supply unit having a lateral flow discharged from the upper side of the dyeing unit, and centrifugal to the outer jaw of the slide seating groove of the rotary staining plate.
- the purpose of this is to form a discharge slope groove so that the specimen of the slide plate is heated quickly and uniformly, and the staining solution and the cleaning solution supplied to the rotating dye plate do not remain so that the distinctive dyeing and cleanliness are maintained.
- Another object of the present invention is to configure the rotary dyeing plate in multiple stages, so that it is possible to dye the specimens of a plurality of slide plates at a time.
- Mycobacterium tuberculosis slide staining apparatus is to visually identify the tuberculosis bacteria through sulfuric acid stain on the slide coated with the specimen collected from the patient.
- Tubercle bacillus slide staining apparatus as described above is a rotary staining plate and a rotating staining plate provided on the inside of the dyeing unit rotated by a motor formed in the dyeing unit formed inside the body as disclosed in Korean Patent No. 1414166
- a dyeing nozzle for applying a dye solution to the slide, a washing nozzle for supplying a washing solution to the slide, a dye plate heater for heating a rotating dye plate, a controller for controlling a dyeing operation of a sample, and a user inputs an operation signal to the controller. It consists of a dyeing control unit that can be done.
- the dyeing heater provided with the rotary dyeing plate is heated so that the specimen is heated through the slide plate. There was a problem that the specimen is dried and uniform heating is not made.
- the conventional Mycobacterium tuberculosis slide staining device is a staining solution remaining in the slide seating groove formed in the rotary staining plate is not clearly removed, and mixed with the secondly supplied dye solution is not a clear staining or contamination of the staining plate There was this.
- the rotary staining plate of the conventional Mycobacterium tuberculosis slide dyeing apparatus is configured to heat the specimen through heating through the slide plate, the heating heater of the specimen is configured to heat the specimen through the slide plate It takes a long time, the specimen is dried and uniform heating is not performed, and the dyeing solution remains in the slide seating groove formed on the rotary dyeing plate. It is intended to solve the problem that is not made or the staining rotary staining plate.
- the Mycobacterium tuberculosis slide dyeing apparatus it is provided with a hot air supply part which is discharged in a lateral direction from the top of the dyeing unit, and a centrifugal discharge inclined groove is formed in the outer jaw of the slide seating groove of the rotary staining plate.
- the rotary dyeing plate is composed of multiple stages.
- the present invention is provided with a hot air supply portion that is laterally discharged discharged from the top of the dyeing section, the heating of the specimen applied to the slide plate is heated by uniform heating from the top of the dyeing section by the convection movement, so that the heating of the specimen is uniform. Clear and warming is not caused by drying, and by forming a centrifugal discharge inclined groove in the outer jaw of the slide seating groove of the rotary staining plate, the dyeing solution and washing solution supplied to the rotary staining plate is centrifugal rotation process of the rotary staining plate Clearly centrifuged and not stained, the staining of specimens is clearly distinguished and dyed in stages, and the cleanliness of the rotating stain plate is maintained.
- the present invention is capable of dyeing the specimens of a plurality of slide plates at a time by configuring a rotary dye plate in multiple stages.
- FIG. 1 is a perspective illustration showing an embodiment according to the present invention.
- Figure 2 is a plan view showing an embodiment according to the present invention.
- Figure 3 is an exemplary view showing that the rotary dyeing plate in a multi-layer in one embodiment according to the present invention.
- Figure 4 is an exemplary view showing that the withdrawal pressing groove in the inner side of the slide seating groove in one embodiment according to the present invention.
- Figure 5 is a detailed illustration of a rotary dye plate showing an embodiment according to the present invention.
- FIG. 6 is a detailed side cross-sectional view according to FIG.
- Figure 7 is a detailed illustration of a rotary dye plate showing another embodiment according to the present invention.
- FIG. 8 is a detailed side cross-sectional view according to FIG. 7.
- the present invention is to ensure that the heating of the specimen applied to the slide plate is made uniform and clear, and that the dye solution and the washing solution supplied to the slide plate is clearly separated and removed from the rotary dye plate.
- a cylindrical dyeing unit 120 is formed therein, and an upper portion of the dyeing unit 120 is formed to be opened and closed by a plate-shaped lid 110 and the dyeing machine body
- the rotary dyeing plate 130 is installed in the center of the dyeing unit 120 to be rotated by the motor 132 installed in the 110, the slide seating groove of the rotary dyeing plate 130 is provided on the inner wall of the dyeing unit 120
- Dye nozzle 142 for spraying the dye solution pumped by the dye solution pump 141 on the slide plate (1) seated on the 131, washing liquid on the slide plate (1) seated in the slide seating groove (131)
- the test object coated on the slide plate 1 seated in the washing nozzle 152 spraying the washing liquid pumped by the pump 151 and the slide seating groove 131 of the rotary dyeing plate 130 of the dyeing unit 120 Circulating soil in the lateral direction from the top of the dyeing unit 120 to be heated by the air conduction from the top Dyeing unit temperature
- the stacking means has a spaced apart stacking portion 133 to be spaced apart on the top of the rotary dyeing plate 130, the rotary dyeing plate 130 is stacked, the slide seating groove 131 alternately stacked, the spaced apart A binding protrusion 133b coupled to the binding groove 133a formed on the lower surface of the rotary dyeing plate 130 stacked on the upper side of the stack is formed.
- the spaced apart laminated portion 133 is configured to be detachable from the rotary dyeing plate 130 and formed so that the outer end thereof is supported so that the slide 1 seated beyond the inner end of the slide seating groove 131 is not separated. It can be done by.
- the spaced apart stacking portion 133 is formed with two or more dye plate coupling grooves 130b to be coupled to two or more dye plate protrusions 130a protruding from the rotary dye plate 130.
- one of the outer and inner ends of the slide seating groove 131 of the rotary dyeing plate 130 is pressed when one end of the seated slide plate 1 is lowered while the other end of the slide plate 1 is lowered.
- One end is lifted to form a pull-out pressing groove (131a) that can be easily withdrawn slide plate (1) can be carried out.
- the centrifugal discharge inclined groove 10 formed to be inclined upwardly to be discharged from the slide seating groove 131 is formed.
- the slide plate 1 when the dyeing solution or washing liquid is buoyantly open when the dyeing or washing liquid is excessively retained on the outer jaw bottom of the slide seating groove 131 of the rotary dyeing plate 130, the slide plate 1 It can be carried out by having a buoyancy open valve 20 to prevent the buoyancy is applied to the).
- the buoyancy opening valve 20 is coupled to the valve chamber 21 formed in the lower portion of the slide seating groove 131 and the buoyancy body 22 accommodated in the valve chamber 21 and the lower portion of the valve chamber 21. And a valve cap 23 forming a valve seat and a valve jaw 24 to prevent the detachment of the buoyancy body 22 above the valve chamber 21. will be.
- the specimen applied to the slide plate 1 seated in the slide seating groove 131 of the rotary staining plate 130 is heated by air conduction at the top of the dyeing unit 120.
- the heating of the specimen applied to the slide plate 1 is rotated dyeing plate 130
- the specimen is heated to a uniform, stable and minimized drying.
- the slide plate 1 seated on the plurality of rotary dyeing plates 130 in one dyeing process. You can dye.
- the rotary dyeing plate 130 is provided with a spaced apart stacking portion 133 that is bound by the binding protrusion 133b and the binding groove 133a, the staggered binding of the rotating dyeing plate 130 is clearly made. It is done.
- the centrifugal discharge inclined groove 10 when the centrifugal discharge inclined groove 10 is formed in the outer jaw of the slide seating groove 131 of the rotary dyeing plate 130, the remaining dye solution or dye supplied to the washing process or Washing liquid is to be centrifuged along the centrifugal discharge inclined groove 10 in the rotation process of the rotary staining plate 130.
- the centrifugal force acts on the inclined groove formed along the centrifugal discharge inclined groove 10 so that the discharge of the dye solution or the washing liquid remaining in the slide seating groove 131 is applied to the specimen applied to the slide plate 1. To be prevented.
- the outer jaw bottom of the rotary dyeing plate 130 is provided with a buoyancy release valve 20, the dyeing or washing liquid in the slide seating groove 131
- the buoyancy body 22 of the buoyancy opening valve 20 is opened while the buoyancy is raised so that the dyeing or washing liquid is quickly discharged through the buoyancy opening valve 20, and the discharge of the dyeing or washing liquid is completed.
- the buoyancy opening valve 20 is closed to prevent the buoyancy from acting on the specimen applied to the slide plate 1 in the rotation process of the rotary dyeing plate 130.
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Abstract
The present invention relates to a tubercular bacillus slide staining apparatus, which uniformly and promptly warms specimens on a slide plate and prevents a staining liquid and a washing liquid, supplied to a rotary staining plate, from remaining, thereby achieving definite differential staining and cleanliness maintenance. That is, in the tubercular bacillus slide staining apparatus of the present invention, a hot air supply unit is provided to discharge a round current in a side direction from above a staining unit, and a centrifugal discharge inclination groove is formed in an outside protrusion of a slide mounting groove of the rotary staining plate.
Description
본 발명은 결핵균 슬라이드 염색장치에 관한 것으로서, 더욱 상세하게는 결핵균 슬라이드 염색장치에 있어서, 염색부의 상부에서 측방향으로 회류 토출되는 열풍공급부를 구비하고, 회전염색판의 슬라이드안착홈의 외측 턱에 원심배출경사홈을 형성하여서, 슬라이드판의 가검물이 신속하고 균일하게 가온되게 하며 회전염색판에 공급된 염색액과 세척액이 잔류되지 않게 하여 명확한 구분 염색과 청결이 유지되도록 함을 목적으로 한 것이다.The present invention relates to a Mycobacterium tuberculosis slide dyeing apparatus, and more particularly, to a Mycobacterium tuberculosis slide dyeing apparatus, comprising a hot air supply unit having a lateral flow discharged from the upper side of the dyeing unit, and centrifugal to the outer jaw of the slide seating groove of the rotary staining plate. The purpose of this is to form a discharge slope groove so that the specimen of the slide plate is heated quickly and uniformly, and the staining solution and the cleaning solution supplied to the rotating dye plate do not remain so that the distinctive dyeing and cleanliness are maintained.
본 발명의 다른 목적은 회전염색판을 다단으로 구성하여서, 한번에 다수의 슬라이드판의 가검물을 염색할 수 있도록 한 것이다.Another object of the present invention is to configure the rotary dyeing plate in multiple stages, so that it is possible to dye the specimens of a plurality of slide plates at a time.
일반적으로, 결핵균 슬라이드 염색장치는 환자로부터 채취된 가검물이 도포된 슬라이드에 황산성염색을 통하여 결핵균을 육안확인 할 수 있도록 하는 것이다.In general, Mycobacterium tuberculosis slide staining apparatus is to visually identify the tuberculosis bacteria through sulfuric acid stain on the slide coated with the specimen collected from the patient.
이상과 같은 결핵균 슬라이드 염색장치는 대한민국 특허 제1416966호에 공개된 된 바와 같이 내부에 염색기몸체의 내부에 형성된 염색부에 모터에 의하여 회전되게 구비된 회전염색판과 상기 염색부의 내벽에서 회전염색판에 거치된 슬라이드에 염색액을 도포하는 염색노즐, 상기 슬라이드에 세정액을 공급하는 세정노즐, 회전염색판을 가열하는 염색판히터, 시료의 염색동작을 제어하는 컨트롤러 및 상기 컨트롤러에 사용자가 조작신호를 입력할 수 있게 한 염색조작부로 구성된 것이다.Tubercle bacillus slide staining apparatus as described above is a rotary staining plate and a rotating staining plate provided on the inside of the dyeing unit rotated by a motor formed in the dyeing unit formed inside the body as disclosed in Korean Patent No. 1414166 A dyeing nozzle for applying a dye solution to the slide, a washing nozzle for supplying a washing solution to the slide, a dye plate heater for heating a rotating dye plate, a controller for controlling a dyeing operation of a sample, and a user inputs an operation signal to the controller. It consists of a dyeing control unit that can be done.
상기한 바와 같은 종래의 결핵균 슬라이드 염색장치는 가검물이 도포된 슬라이드를 회전염색판에 거치한 후 조작부를 조작하여 염색작업을 실시하게 되면, 상기 컨트롤러가 회전염색판에 구비된 염색히터를 동작시켜 슬라이드를 일정온도로 가열한다.Conventional Mycobacterium tuberculosis slide staining apparatus as described above is when the dye is applied to the rotating dye plate by mounting the slide coated with the specimen and the operation unit to perform the dyeing operation, the controller operates the dye heater provided on the rotating dye plate slide Heat to constant temperature.
이상과 같이 가검물의 가열이 이루어진 슬라이드에 염색노즐에서 염색약을 도포한 후 염색이 되게 하고 염색이 완료되면 세척노즐에서 세척액을 분무하는 반복작업을 통하여 염색을 하게 되는 것이다.As described above, after applying the dye in the dyeing nozzle on the slide made of the heating of the specimen to be dyed and the dyeing is completed, the dyeing through the repeated operation of spraying the cleaning solution in the washing nozzle.
그러나, 상기한 바와 같은 종래의 결핵균 슬라이드 염색장치의 회전염색판이 가검물의 가열이 회전염색판에 구비된 염색히터가 슬라이드판을 통한 전도를 통하여 가검물을 가열하게 구성되어 있어 그 가열에 많은 시간이 소요되고 가검물이 건조되며 균일한 가열이 이루어지지 않는 문제점이 있었다.However, as described above, in the conventional dye-staining plate of the Mycobacterium tuberculosis slide dyeing apparatus, the dyeing heater provided with the rotary dyeing plate is heated so that the specimen is heated through the slide plate. There was a problem that the specimen is dried and uniform heating is not made.
또한, 종래의 결핵균 슬라이드 염색장치는 회전염색판에 형성된 슬라이드안착홈에 염색액이 잔류하여 명확하게 제거되지 않고 2차적으로 공급된 염색액과 믹싱되어 명확한 염색이 이루어지지 않거나 회전염색판이 오염되는 문제점이 있었다.In addition, the conventional Mycobacterium tuberculosis slide staining device is a staining solution remaining in the slide seating groove formed in the rotary staining plate is not clearly removed, and mixed with the secondly supplied dye solution is not a clear staining or contamination of the staining plate There was this.
이에, 본 발명은 상술한 바와 같이 종래의 결핵균 슬라이드 염색장치의 회전염색판이 가검물의 가열이 회전염색판에 구비된 염색히터가 슬라이드판을 통한 전도를 통하여 가검물을 가열하게 구성되어 있어 그 가열에 많은 시간이 소요되고 가검물이 건조되며 균일한 가열이 이루어지지 않는 문제점과, 회전염색판에 형성된 슬라이드안착홈에 염색액이 잔류하여 명확하게 제거되지 않고 2차적으로 공급된 염색액과 믹싱되어 명확한 염색이 이루어지지 않거나 회전염색판이 오염되는 문제점을 해결할 수 있도록 한 것이다.Thus, the present invention, as described above, the rotary staining plate of the conventional Mycobacterium tuberculosis slide dyeing apparatus is configured to heat the specimen through heating through the slide plate, the heating heater of the specimen is configured to heat the specimen through the slide plate It takes a long time, the specimen is dried and uniform heating is not performed, and the dyeing solution remains in the slide seating groove formed on the rotary dyeing plate. It is intended to solve the problem that is not made or the staining rotary staining plate.
즉, 본 발명은 결핵균 슬라이드 염색장치에 있어서, 염색부의 상부에서 측방향으로 회류 토출되는 열풍공급부를 구비하고, 회전염색판의 슬라이드안착홈의 외측 턱에 원심배출경사홈을 형성한 것이다. That is, in the Mycobacterium tuberculosis slide dyeing apparatus, it is provided with a hot air supply part which is discharged in a lateral direction from the top of the dyeing unit, and a centrifugal discharge inclined groove is formed in the outer jaw of the slide seating groove of the rotary staining plate.
또한, 상기 회전염색판을 다단으로 구성한 것이다.In addition, the rotary dyeing plate is composed of multiple stages.
따라서, 본 발명은 염색부의 상부에서 측방향으로 회류 토출되는 열풍공급부를 구비함으로써 슬라이드판에 도포된 가검물의 가온이 대류이동에 의하여 염색부의 상부로부터 균일한 가온에 의하여 가열되어 가검물의 가온이 균일하고 명확하며 건조를 유발하지 않고 가온이 이루어지고, 회전염색판의 슬라이드안착홈의 외측 턱에 원심배출경사홈을 형성함으로써, 회전염색판에 공급된 염색액과 세척액이 회전염색판의 원심분리 회전과정에 잔류되지 않고 명확하게 원심분리되어 가검물의 염색이 단계별로 명확하게 구분 염색되며 회전염색판의 청결이 유지되는 것이다.Therefore, the present invention is provided with a hot air supply portion that is laterally discharged discharged from the top of the dyeing section, the heating of the specimen applied to the slide plate is heated by uniform heating from the top of the dyeing section by the convection movement, so that the heating of the specimen is uniform. Clear and warming is not caused by drying, and by forming a centrifugal discharge inclined groove in the outer jaw of the slide seating groove of the rotary staining plate, the dyeing solution and washing solution supplied to the rotary staining plate is centrifugal rotation process of the rotary staining plate Clearly centrifuged and not stained, the staining of specimens is clearly distinguished and dyed in stages, and the cleanliness of the rotating stain plate is maintained.
또한, 본 발명은 회전염색판을 다단으로 구성함으로써 한번에 다수의 슬라이드판의 가검물을 염색할 수 있는 것이다.In addition, the present invention is capable of dyeing the specimens of a plurality of slide plates at a time by configuring a rotary dye plate in multiple stages.
도 1 은 본 발명에 따른 일 실시 예를 보인 사시 예시도.1 is a perspective illustration showing an embodiment according to the present invention.
도 2 는 본 발명에 따른 일 실시 예를 보인 평면 예시도.Figure 2 is a plan view showing an embodiment according to the present invention.
도 3 은 본 발명에 따른 일 실시 예에 있어 회전염색판을 복층으로 구비한 것을 보인 예시도.Figure 3 is an exemplary view showing that the rotary dyeing plate in a multi-layer in one embodiment according to the present invention.
도 4 는 본 발명에 따른 일 실시 예에 있어 인출누름홈을 슬라이드안착홈의 내측에 구비한 것을 보인 예시도.Figure 4 is an exemplary view showing that the withdrawal pressing groove in the inner side of the slide seating groove in one embodiment according to the present invention.
도 5 는 본 발명에 따른 일 실시 예를 보인 회전염색판의 상세 예시도.Figure 5 is a detailed illustration of a rotary dye plate showing an embodiment according to the present invention.
도 6 은 도 5에 따른 상세 측단면 예시도.6 is a detailed side cross-sectional view according to FIG.
도 7 은 본 발명에 따른 다른 실시 예를 보인 회전염색판의 상세 예시도.Figure 7 is a detailed illustration of a rotary dye plate showing another embodiment according to the present invention.
도 8 은 도 7 에 따른 상세 측 단면 예시도.8 is a detailed side cross-sectional view according to FIG. 7.
도 9 는 본 발명에 따른 주요 구성도.9 is a main configuration according to the present invention.
이하, 첨부된 도면에 의하여 상세히 설명하면 다음과 같다.Hereinafter, described in detail by the accompanying drawings as follows.
본 발명은 슬라이드판에 도포된 가검물의 가온이 균일하고 명확하게 이루어지도록 하며, 슬라이드판에 공급된 염색액 및 세척액이 회전염색판에서 명확하게 분리 제거 되도록 한 것이다.The present invention is to ensure that the heating of the specimen applied to the slide plate is made uniform and clear, and that the dye solution and the washing solution supplied to the slide plate is clearly separated and removed from the rotary dye plate.
즉, 본 발명은 결핵균 슬라이드 염색장치에 있어서, 내부에 원통형의 염색부(120)가 형성되고 상기 염색부(120)의 상부가 판형 뚜껑에 의하여 개폐되게 형성된 염색기몸체(110)와 상기 염색기몸체(110)에 설치된 모터(132)에 의하여 회전되게 염색부(120)의 중앙에 설치되는 회전염색판(130), 상기 염색부(120)의 내벽에 구비되어 회전염색판(130)의 슬라이드안착홈(131)에 안착된 슬라이드판(1)에 염색액펌프(141)에 의하여 펌핑된 염색액을 분무하는 염색노즐(142), 상기 슬라이드안착홈(131)에 안착된 슬라이드판(1)에 세척액펌프(151)에 의하여 펌핑된 세척액을 분무하는 세척노즐(152), 회전염색판(130)의 슬라이드안착홈(131)에 안착된 슬라이드판(1)에 도포된 가검물을 염색부(120)의 상부에서 공기전도에 의하여 가열할 수 있게 염색부(120)의 상부에서 측방향으로 회류 토출되는 열풍공급부(161), 상기 염색부(120)의 내벽에 상기 열풍공급부(161)에 의하여 공급된 열풍에 의하여 가온 된 염색부(120)의 온도를 감지하여 염색부온도센서(162), 모터(132)와 펌프 및 열풍공급부(161)의 동작을 제어하는 컨트롤러(171) 및 상기 컨트롤러(171)에 사용자가 조작신호를 입력할 수 있게 한 염색기몸체(110)의 외부로 노출되게 구비된 염색조작부(172)로 구성된 것이다.That is, in the Mycobacterium tuberculosis slide dyeing apparatus, a cylindrical dyeing unit 120 is formed therein, and an upper portion of the dyeing unit 120 is formed to be opened and closed by a plate-shaped lid 110 and the dyeing machine body ( The rotary dyeing plate 130 is installed in the center of the dyeing unit 120 to be rotated by the motor 132 installed in the 110, the slide seating groove of the rotary dyeing plate 130 is provided on the inner wall of the dyeing unit 120 Dye nozzle 142 for spraying the dye solution pumped by the dye solution pump 141 on the slide plate (1) seated on the 131, washing liquid on the slide plate (1) seated in the slide seating groove (131) The test object coated on the slide plate 1 seated in the washing nozzle 152 spraying the washing liquid pumped by the pump 151 and the slide seating groove 131 of the rotary dyeing plate 130 of the dyeing unit 120 Circulating soil in the lateral direction from the top of the dyeing unit 120 to be heated by the air conduction from the top Dyeing unit temperature sensor 162, by detecting the temperature of the dyeing unit 120 is heated by the hot air supplied by the hot air supply unit 161 on the inner wall of the hot air supply unit 161, the dyeing unit 120 132 and the controller 171 for controlling the operation of the pump and hot air supply unit 161 and the dyeing is provided to be exposed to the outside of the dyeing machine body 110 that allows the user to input the operation signal to the controller 171 The operation unit 172 is configured.
한편, 본 발명의 실시에 있어서 회전염색판(130)을 적층수단에 의하여 다단으로 적층되게 구성하여 실시할 수 있는 것이다.On the other hand, in the practice of the present invention can be carried out by configuring the rotary dyeing plate 130 is laminated in multiple stages by the lamination means.
상기 적층수단은 회전염색판(130)의 상부에는 상측으로 적층되는 회전염색판(130)이 이격되되 슬라이드안착홈(131)이 엇갈리게 적층결합되게 하는 이격 적층부(133)를 구비하고, 상기 이격 적층부 상부에는 상측으로 적층되는 회전염색판(130)의 하면에 형성된 결속홈(133a)에 결합되는 결속돌기(133b)가 형성된 것이다.The stacking means has a spaced apart stacking portion 133 to be spaced apart on the top of the rotary dyeing plate 130, the rotary dyeing plate 130 is stacked, the slide seating groove 131 alternately stacked, the spaced apart A binding protrusion 133b coupled to the binding groove 133a formed on the lower surface of the rotary dyeing plate 130 stacked on the upper side of the stack is formed.
한편, 상기 이격 적층부(133)는 회전염색판(130)에서 분리 가능하게 구성하고 그 외측 단부가 슬라이드안착홈(131)의 내측 단부를 넘어서서 안착된 슬라이드(1)가 이탈되지 않도록 지지되게 형성하여 실시할 수 있는 것이다.On the other hand, the spaced apart laminated portion 133 is configured to be detachable from the rotary dyeing plate 130 and formed so that the outer end thereof is supported so that the slide 1 seated beyond the inner end of the slide seating groove 131 is not separated. It can be done by.
상기 이격 적층부(133)는 회전염색판(130)에서 돌출형성된 두 개 이상의 염색판돌기(130a)와 결합되어는 두 개 이상의 염색판결합홈(130b)이 형성된 것이다.The spaced apart stacking portion 133 is formed with two or more dye plate coupling grooves 130b to be coupled to two or more dye plate protrusions 130a protruding from the rotary dye plate 130.
그리고, 상기 회전염색판(130)의 슬라이드안착홈(131)의 외측과 내측 단부 중 어느 일측에는 안착된 슬라이드판(1)의 일단을 눌렀을 때 눌려지 슬라이드판(1)의 일단이 하강 되면서 다른 일단이 들어 올려져 슬라이드판(1)을 용이하게 인출할 수 있게 한 인출누름홈(131a)을 형성하여 실시할 수 있는 것이다.In addition, one of the outer and inner ends of the slide seating groove 131 of the rotary dyeing plate 130 is pressed when one end of the seated slide plate 1 is lowered while the other end of the slide plate 1 is lowered. One end is lifted to form a pull-out pressing groove (131a) that can be easily withdrawn slide plate (1) can be carried out.
또한, 상기 회전염색판(130)의 슬라이드안착홈(131) 외측 턱에는 염색 및 세척과정에 공급된 잔류 염색액 또는 세척액을 슬라이드판(1)에 도포된 가검물의 염색상태에 부압이 작용되지 않도록 슬라이드안착홈(131)에서 배출시킬 수 있게 상향 경사지게 형성한 원심배출경사홈(10)을 형성한 것이다.In addition, in the outer jaw of the slide seating groove 131 of the rotary dyeing plate 130, the residual dye solution or the cleaning solution supplied during the dyeing and washing process so that the negative pressure is not applied to the dyeing state of the specimen applied to the slide plate (1). The centrifugal discharge inclined groove 10 formed to be inclined upwardly to be discharged from the slide seating groove 131 is formed.
또한, 본 발명의 실시에 있어서 회전염색판(130)의 슬라이드안착홈(131) 외측 턱 저면에는 염색액 또는 세척액이 과도 잔류시 부력 개방되고 염색액 또는 세척액이 잔류하지 않을 경우에는 슬라이드판(1)에 부력이 작용되지 않도록 하는 부력개방밸브(20)를 구비하여 실시할 수 있는 것이다.In addition, in the practice of the present invention, when the dyeing solution or washing liquid is buoyantly open when the dyeing or washing liquid is excessively retained on the outer jaw bottom of the slide seating groove 131 of the rotary dyeing plate 130, the slide plate 1 It can be carried out by having a buoyancy open valve 20 to prevent the buoyancy is applied to the).
상기 부력개방밸브(20)는 슬라이드안착홈(131)의 하부에서 천공형성된 밸브실(21)과 상기 밸브실(21)에 수용되는 부력체(22), 상기 밸브실(21)의 하부에 결합되어 부력체(22)의 하부 이탈이 방지되게 하고 밸브시트를 형성하는 밸브캡(23) 및 상기 밸브실(21)의 상측으로 부력체(22)의 이탈이 방지되게 밸브턱(24)으로 구성된 것이다.The buoyancy opening valve 20 is coupled to the valve chamber 21 formed in the lower portion of the slide seating groove 131 and the buoyancy body 22 accommodated in the valve chamber 21 and the lower portion of the valve chamber 21. And a valve cap 23 forming a valve seat and a valve jaw 24 to prevent the detachment of the buoyancy body 22 above the valve chamber 21. will be.
이하, 본 발명의 사용과정에 대하여 설명하면 다음과 같다.Hereinafter, the use process of the present invention will be described.
상기한 바와 같이 결핵균 슬라이드 염색장치에 있어서, 회전염색판(130)의 슬라이드안착홈(131)에 안착된 슬라이드판(1)에 도포된 가검물을 염색부(120)의 상부에서 공기전도에 의하여 가열할 수 있게 염색부(120)의 상부에서 측 방향으로 회류 토출되는 열풍공급부(161)로 구성한 본 발명을 적용하여 실시하게 되면, 슬라이드판(1)에 도포된 가검물의 가열이 회전염색판(130)을 통한 전도에 의한 것이 아니고 공기의 대류 유동에 따라 상측부터 가열된 염색부(120)의 공기 전도에 의하여 가검물의 가열이 이루어져 가검물이 균일하고 안정적이며 건조가 최소화된 상태로 가온 되는 것이다.In the Mycobacterium tuberculosis slide dyeing apparatus as described above, the specimen applied to the slide plate 1 seated in the slide seating groove 131 of the rotary staining plate 130 is heated by air conduction at the top of the dyeing unit 120. When applied to the present invention composed of the hot air supply unit 161 is discharged in a lateral direction from the top of the dyeing unit 120 so as to be implemented, the heating of the specimen applied to the slide plate 1 is rotated dyeing plate 130 By the conduction of air through the air conduction of the dyeing unit 120 heated from the upper side according to the convection flow of the air through the heating), the specimen is heated to a uniform, stable and minimized drying.
또한, 본 발명의 실시에 있어서 회전염색판(130)을 적층수단에 의하여 다단으로 적층되게 구성하여 실시하게 되면 한 번의 염색과정에 있어 다수의 회전염색판(130)에 안착된 슬라이드판(1)을 염색할 수 있는 것이다.In addition, in the practice of the present invention, if the rotary dyeing plate 130 is configured to be stacked in multiple stages by lamination means, the slide plate 1 seated on the plurality of rotary dyeing plates 130 in one dyeing process. You can dye.
또한, 상기 회전염색판(130)이 결속돌기(133b)와 결속홈(133a)에 의하여 결속되는 이격 적층부(133)를 구비하여 실시하게 되면, 회전염색판(130)의 엇갈림 결속이 명확하게 이루어지는 것이다.In addition, when the rotary dyeing plate 130 is provided with a spaced apart stacking portion 133 that is bound by the binding protrusion 133b and the binding groove 133a, the staggered binding of the rotating dyeing plate 130 is clearly made. It is done.
또한, 상기 회전염색판(130)의 슬라이드안착홈(131)의 외측과 내측 단부 중 어느 일 측에 인출누름홈(131a)을 형성하여 실시하게 되면, 슬라이드안착홈(131)에 안착된 슬라이드판(1)을 용이하게 인출할 수 있는 것이다.In addition, when the extraction pressing groove 131a is formed on either side of the outer side and the inner end of the slide seating groove 131 of the rotary dye plate 130, the slide plate seated in the slide seating groove 131 (1) can be easily withdrawn.
또한, 본 발명의 실시에 있어서, 회전염색판(130)의 슬라이드안착홈(131) 외측 턱에 원심배출경사홈(10)을 형성하여 실시하게 되면, 염색 및 세척과정에 공급된 잔류 염색액 또는 세척액이 회전염색판(130)의 회전과정에 있어 상기 원심배출경사홈(10)을 따라 원심배출되는 것이다.In addition, in the practice of the present invention, when the centrifugal discharge inclined groove 10 is formed in the outer jaw of the slide seating groove 131 of the rotary dyeing plate 130, the remaining dye solution or dye supplied to the washing process or Washing liquid is to be centrifuged along the centrifugal discharge inclined groove 10 in the rotation process of the rotary staining plate 130.
이때, 상기 슬라이드안착홈(131)에 잔류한 염색액 또는 세척액의 배출이 원심배출경사홈(10)을 따라 형성되는 경사홈으로 원심력이 작용되어 슬라이드판(1)에 도포된 가검물에 원심력이 작용되는 것이 방지되는 것이다.At this time, the centrifugal force acts on the inclined groove formed along the centrifugal discharge inclined groove 10 so that the discharge of the dye solution or the washing liquid remaining in the slide seating groove 131 is applied to the specimen applied to the slide plate 1. To be prevented.
또한, 본 발명의 실시에 있어서 회전염색판(130)의 슬라이드안착홈(131) 외측 턱 저면에는 부력개방밸브(20)를 구비하여 실시하게 되면, 슬라이드안착홈(131)에 염색액 또는 세척액이 과도 잔류시 상기 부력개방밸브(20)의 부력체(22)가 부력 상승 되면서 개방되어 염색액 또는 세척액이 상기 부력개방밸브(20)를 통하여 신속하게 배출되고, 상기 염색액 또는 세척액의 배출이 완료되게 되면 상기 부력개방밸브(20)가 밀폐되어 회전염색판(130)의 회전과정에 있어 슬라이드판(1)에 도포된 가검물에 부력이 작용되는 것이 방지되는 것이다.In addition, in the practice of the present invention, if the bottom of the slide seating groove 131, the outer jaw bottom of the rotary dyeing plate 130 is provided with a buoyancy release valve 20, the dyeing or washing liquid in the slide seating groove 131 In case of excessive residual, the buoyancy body 22 of the buoyancy opening valve 20 is opened while the buoyancy is raised so that the dyeing or washing liquid is quickly discharged through the buoyancy opening valve 20, and the discharge of the dyeing or washing liquid is completed. When the buoyancy opening valve 20 is closed to prevent the buoyancy from acting on the specimen applied to the slide plate 1 in the rotation process of the rotary dyeing plate 130.
Claims (4)
- 결핵균 슬라이드 염색장치에 있어서;In Mycobacterium tuberculosis slide staining apparatus;내부에 원통형의 염색부(120)가 형성되고 상기 염색부(120)의 상부가 판형 뚜껑에 의하여 개폐되게 형성된 염색기몸체(110)와 상기 염색기몸체(110)에 설치된 모터(132)에 의하여 회전되게 염색부(120)의 중앙에 설치되는 회전염색판(130), 상기 염색부(120)의 내벽에 구비되어 회전염색판(130)의 슬라이드안착홈(131)에 안착된 슬라이드판(1)에 염색액펌프(141)에 의하여 펌핑된 염색액을 분무하는 염색노즐(142), 상기 슬라이드안착홈(131)에 안착된 슬라이드판(1)에 세척액펌프(151)에 의하여 펌핑된 세척액을 분무하는 세척노즐(152), 회전염색판(130)의 슬라이드안착홈(131)에 안착된 슬라이드판(1)에 도포된 가검물을 염색부(120)의 상부에서 공기전도에 의하여 가열할 수 있게 염색부(120)의 상부에서 측방향으로 회류 토출되는 열풍공급부(161), 상기 염색부(120)의 내벽에 상기 열풍공급부(161)에 의하여 공급된 열풍에 의하여 가온 된 염색부(120)의 온도를 감지하여 염색부온도센서(162), 모터(132)와 펌프 및 열풍공급부(161)의 동작을 제어하는 컨트롤러(171) 및 상기 컨트롤러(171)에 사용자가 조작신호를 입력할 수 있게 한 염색기몸체(110)의 외부로 노출되게 구비된 염색조작부(172)로 구성되고,Cylindrical dyeing unit 120 is formed therein so that the upper portion of the dyeing unit 120 is rotated by the dyeing machine body 110 and the motor 132 installed on the dyeing machine body 110 formed to be opened and closed by a plate-shaped lid. The rotary dyeing plate 130 is installed in the center of the dyeing unit 120, provided on the inner wall of the dyeing unit 120 to the slide plate (1) seated in the slide seating groove 131 of the rotary dyeing plate 130 Spraying the washing solution pumped by the washing solution pump 151 to the dyeing nozzle 142 for spraying the dye solution pumped by the dye solution pump 141, the slide plate (1) seated in the slide seating groove 131 Dyeing unit to be heated by the air conduction on the upper part of the dyeing unit 120, the test article applied to the slide plate 1 seated in the washing nozzle 152, the slide seating groove 131 of the rotary dyeing plate 130 Hot air supply 161 is discharged in a lateral direction from the top of the 120, the image on the inner wall of the dyeing unit 120 By detecting the temperature of the dyeing unit 120 warmed by the hot air supplied by the hot air supply unit 161 to control the operation of the dyeing unit temperature sensor 162, the motor 132 and the pump and hot air supply unit 161 Consists of a dyeing operation unit 172 is provided to be exposed to the controller 171 and the dyeing machine body 110 that allows the user to input the operation signal to the controller 171,상기 회전염색판(130)의 슬라이드안착홈(131)의 외측과 내측 단부 중 어느 일측에는 안착된 슬라이드판(1)의 일단을 눌렀을 때 눌려진 슬라이드판(1)의 일단이 하강 되면서 다른 일단이 들어 올려져 슬라이드판(1)을 용이하게 인출할 수 있게 한 인출누름홈(131a)을 형성한 것을 특징으로 하는 결핵균 슬라이드 염색장치.On one side of the outer and inner ends of the slide seating groove 131 of the rotary dyeing plate 130, one end of the slide plate (1) pressed when one end of the seated slide plate (1) is lowered, the other end is entered Mycobacterium tuberculosis slide staining apparatus, characterized in that the pull-out pressing groove (131a) formed to be easily lifted withdraw the slide plate (1).
- 제 1 항에 있어서;The method of claim 1;회전염색판(130)을 적층수단에 의하여 다단으로 적층되게 구성하되,The rotary dyeing plate 130 is configured to be laminated in multiple stages by lamination means,상기 적층수단은 회전염색판(130)의 상부에는 상측으로 적층되는 회전염색판(130)이 이격되되 슬라이드안착홈(131)이 엇갈리게 적층결합되게 하는 이격 적층부(133)를 구비하고, 상기 이격 적층부 상부에는 상측으로 적층되는 회전염색판(130)의 하면에 형성된 결속홈(133a)에 결합되는 결속돌기(133b)가 형성한 것을 특징으로 하는 결핵균 슬라이드 염색장치.The stacking means has a spaced apart stacking portion 133 to be spaced apart on the top of the rotary dyeing plate 130, the rotary dyeing plate 130 is stacked, the slide seating groove 131 alternately stacked, the spaced apart Mycobacterium tuberculosis slide dyeing apparatus characterized in that the binding protrusions (133b) formed on the upper portion of the laminated portion is coupled to the binding groove (133a) formed on the lower surface of the rotary dyeing plate 130 is stacked on the upper side.
- 제 1 항에 있어서;The method of claim 1;상기 이격 적층부(133)은 회전염색판(130)에서 분리 가능하게 구성하고 그 외측 단부가 슬라이드안착홈(131)의 내측 단부를 넘어서서 안착된 슬라이드(1)가 이탈되지 않도록 지지되게 형성하고,The spaced apart stacking portion 133 is configured to be detachable from the rotary dyeing plate 130 and formed so that the outer end thereof is supported beyond the inner end of the slide seating groove 131 so as not to be released from the slide 1,상기 이격 적층부(133)는 회전염색판(130)에서 돌출형성된 두 개 이상의 염색판돌기(130a)와 결합되어는 두 개 이상의 염색판결합홈(130b)이 형성된 것을 특징으로 하는 결핵균 슬라이드 염색장치.The spacing layer 133 is a tuberculosis slide staining apparatus, characterized in that formed with two or more dye plate coupling grooves (130b) is coupled to two or more dye plate projections (130a) protruding from the rotary staining plate 130 .
- 제 1항에 있어서;The method of claim 1;상기 회전염색판(130)의 슬라이드안착홈(131) 외측 턱에는 염색 및 세척과정에 공급된 잔류 염색액 또는 세척액을 슬라이드판(1)에 도포된 가검물의 염색상태에 부압이 작용되지 않도록 슬라이드안착홈(131)에서 배출시킬 수 있게 상향 경사지게 형성한 원심배출경사홈(10)을 형성하고,On the outer jaw of the slide seating groove 131 of the rotary dyeing plate 130, the slide is placed so that the negative pressure is not applied to the dyeing state of the specimen applied to the slide plate 1 with the residual dye solution or washing solution supplied in the dyeing and washing process. Forming a centrifugal discharge inclined groove 10 formed to be inclined upwardly to be discharged from the groove 131,회전염색판(130)의 슬라이드안착홈(131) 외측 턱 저면에는 염색액 또는 세척액이 과도 잔류시 부력 개방되고 염색액 또는 세척액이 잔류하지 않을 경우에는 슬라이드판(1)에 부력이 작용되지 않도록 하는 부력개방밸브(20)를 구비하되,When the dyeing solution or washing liquid is buoyantly open on the bottom of the outer jaw bottom of the slide seating groove 131 of the rotary dyeing plate 130, the buoyancy is not applied to the slide plate 1 when the dyeing or washing liquid does not remain. A buoyancy open valve 20 is provided,상기 부력개방밸브(20)는 슬라이드안착홈(131)의 하부에서 천공형성된 밸브실(21)과 상기 밸브실(21)에 수용되는 부력체(22), 상기 밸브실(21)의 하부에 결합되어 부력체(22)의 하부 이탈이 방지되게 하고 밸브시트를 형성하는 밸브캡(23) 및 상기 밸브실(21)의 상측으로 부력체(22)의 이탈이 방지되게 밸브턱(24)으로 구성된 것을 특징으로 하는 결핵균 슬라이드 염색장치.The buoyancy opening valve 20 is coupled to the valve chamber 21 formed in the lower portion of the slide seating groove 131 and the buoyancy body 22 accommodated in the valve chamber 21 and the lower portion of the valve chamber 21. And a valve cap 23 forming a valve seat and a valve jaw 24 to prevent the detachment of the buoyancy body 22 above the valve chamber 21. Mycobacterium tuberculosis slide staining apparatus, characterized in that.
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| Application Number | Priority Date | Filing Date | Title |
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| US15/544,920 US10184863B2 (en) | 2014-10-30 | 2015-05-13 | Tubercular bacillus slide staining apparatus |
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| KR1020150010864A KR101579231B1 (en) | 2014-10-30 | 2015-01-22 | TB slide staining device |
| KR10-2015-0010864 | 2015-01-22 |
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