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WO2016035099A1 - Dispositif à flux traversant pour la détection de substances biologiques à analyser et procédé associé - Google Patents

Dispositif à flux traversant pour la détection de substances biologiques à analyser et procédé associé Download PDF

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Publication number
WO2016035099A1
WO2016035099A1 PCT/IN2015/000347 IN2015000347W WO2016035099A1 WO 2016035099 A1 WO2016035099 A1 WO 2016035099A1 IN 2015000347 W IN2015000347 W IN 2015000347W WO 2016035099 A1 WO2016035099 A1 WO 2016035099A1
Authority
WO
WIPO (PCT)
Prior art keywords
membrane
flow
antigens
group
detection
Prior art date
Application number
PCT/IN2015/000347
Other languages
English (en)
Inventor
Pramod Kumar Minocha
Rajnikant Gandalal Vyas
Shailesh Yashwant PARAB
Nilay Mohanlal LAD
Original Assignee
Meril Diagnostics Private Limited
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Meril Diagnostics Private Limited filed Critical Meril Diagnostics Private Limited
Publication of WO2016035099A1 publication Critical patent/WO2016035099A1/fr

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54386—Analytical elements
    • G01N33/54387—Immunochromatographic test strips
    • G01N33/54391—Immunochromatographic test strips based on vertical flow
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56983—Viruses
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
    • G01N2333/01—DNA viruses
    • G01N2333/02—Hepadnaviridae, e.g. hepatitis B virus
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
    • G01N2333/01—DNA viruses
    • G01N2333/03—Herpetoviridae, e.g. pseudorabies virus
    • G01N2333/035—Herpes simplex virus I or II
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
    • G01N2333/01—DNA viruses
    • G01N2333/03—Herpetoviridae, e.g. pseudorabies virus
    • G01N2333/04—Varicella-zoster virus
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
    • G01N2333/01—DNA viruses
    • G01N2333/03—Herpetoviridae, e.g. pseudorabies virus
    • G01N2333/04—Varicella-zoster virus
    • G01N2333/045—Cytomegalovirus
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
    • G01N2333/01—DNA viruses
    • G01N2333/075—Adenoviridae
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
    • G01N2333/08—RNA viruses
    • G01N2333/115—Paramyxoviridae, e.g. parainfluenza virus
    • G01N2333/12—Mumps virus; Measles virus
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
    • G01N2333/08—RNA viruses
    • G01N2333/115—Paramyxoviridae, e.g. parainfluenza virus
    • G01N2333/135—Respiratory syncytial virus
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
    • G01N2333/08—RNA viruses
    • G01N2333/15—Retroviridae, e.g. bovine leukaemia virus, feline leukaemia virus, feline leukaemia virus, human T-cell leukaemia-lymphoma virus
    • G01N2333/155—Lentiviridae, e.g. visna-maedi virus, equine infectious virus, FIV, SIV
    • G01N2333/16—HIV-1, HIV-2
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
    • G01N2333/08—RNA viruses
    • G01N2333/18—Togaviridae; Flaviviridae
    • G01N2333/183—Flaviviridae, e.g. pestivirus, mucosal disease virus, bovine viral diarrhoea virus, classical swine fever virus (hog cholera virus) or border disease virus
    • G01N2333/186—Hepatitis C; Hepatitis NANB
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
    • G01N2333/20—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Spirochaetales (O), e.g. Treponema, Leptospira
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
    • G01N2333/285—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Pasteurellaceae (F), e.g. Haemophilus influenza

Definitions

  • the proposed invention also provides a method of detection with 100 % sensitivity and > 99.5% specificity.
  • the disease specific biomolecule immobilized on the membrane will covalently bind with the disease specific bioanalyte in the patient's sample.
  • the assay diluent is added once again to remove any unbound bioanalytes along with those weakly bound or bound with low affinity and other particulates from the membrane which in turn get soaked through the membrane and settle into the absorbent pad.

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

La présente invention concerne un dispositif à flux traversant permettant la détection et la différenciation de multiples substances biologiques à analyser à partir d'un seul échantillon et un procédé associé. Le dispositif est un automate d'immunoanalyse visuel, rapide, sensible et fiable pour la détection différentielle de multiples substances biologiques à analyser dans des liquides organiques comme le sang, le sérum ou le plasma humain par l'immobilisation des biomolécules spécifiques de la maladie respectives, c'est-à-dire antigène, anticorps, peptides, peptides de synthèse ou protéines recombinantes dérivés de micro-organismes provoquant une maladie sur la membrane de réaction immunofiltration.
PCT/IN2015/000347 2014-09-05 2015-09-07 Dispositif à flux traversant pour la détection de substances biologiques à analyser et procédé associé WO2016035099A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IN2847MU2014 2014-09-05
IN2847/MUM/2014 2014-09-05

Publications (1)

Publication Number Publication Date
WO2016035099A1 true WO2016035099A1 (fr) 2016-03-10

Family

ID=54834880

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IN2015/000347 WO2016035099A1 (fr) 2014-09-05 2015-09-07 Dispositif à flux traversant pour la détection de substances biologiques à analyser et procédé associé

Country Status (1)

Country Link
WO (1) WO2016035099A1 (fr)

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999056128A1 (fr) * 1998-04-30 1999-11-04 Universal Healthwatch, Inc. Dosage immunitaire avec peptides mixtes
WO2001095784A2 (fr) * 2000-06-14 2001-12-20 J.Mitra & Co. Ltd. Trousse de diagnostic destinee a la detection in vitro de l'hepatite c
US20030165970A1 (en) * 2001-08-17 2003-09-04 Gengxi Hu Diagnostic kit for simultaneously detecting multiple infectious diseases and the preparation thereof
CA2578313A1 (fr) * 2007-03-01 2007-05-14 Biolytical Laboratories Inc. Dispositif de dosage immunologique parallele rapide et tres precis
US20090263825A1 (en) * 2005-06-21 2009-10-22 The Government of the United States of America as Represented by the Secretary of Methods, immunoassays and devices for detection of anti-lipoidal antibodies
WO2013178737A1 (fr) * 2012-05-30 2013-12-05 Bio-Rad Innovations Procédé pour diagnostiquer et différencier des infections par le vih-2

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999056128A1 (fr) * 1998-04-30 1999-11-04 Universal Healthwatch, Inc. Dosage immunitaire avec peptides mixtes
WO2001095784A2 (fr) * 2000-06-14 2001-12-20 J.Mitra & Co. Ltd. Trousse de diagnostic destinee a la detection in vitro de l'hepatite c
US20030165970A1 (en) * 2001-08-17 2003-09-04 Gengxi Hu Diagnostic kit for simultaneously detecting multiple infectious diseases and the preparation thereof
US20090263825A1 (en) * 2005-06-21 2009-10-22 The Government of the United States of America as Represented by the Secretary of Methods, immunoassays and devices for detection of anti-lipoidal antibodies
CA2578313A1 (fr) * 2007-03-01 2007-05-14 Biolytical Laboratories Inc. Dispositif de dosage immunologique parallele rapide et tres precis
WO2013178737A1 (fr) * 2012-05-30 2013-12-05 Bio-Rad Innovations Procédé pour diagnostiquer et différencier des infections par le vih-2

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
OPERSKALSKI EA, CURRENT HIV/AIDS REPORT, vol. 8, no. 1, March 2011 (2011-03-01), pages 12 - 22

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