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WO2016033251A3 - Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation - Google Patents

Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation Download PDF

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Publication number
WO2016033251A3
WO2016033251A3 PCT/US2015/047053 US2015047053W WO2016033251A3 WO 2016033251 A3 WO2016033251 A3 WO 2016033251A3 US 2015047053 W US2015047053 W US 2015047053W WO 2016033251 A3 WO2016033251 A3 WO 2016033251A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
compositions
methods
high efficiency
acid sequence
Prior art date
Application number
PCT/US2015/047053
Other languages
French (fr)
Other versions
WO2016033251A2 (en
Inventor
Doug AMORESE
Nurith Kurn
Jonathan Scolnick
Alexandra Hui WANG
Original Assignee
Nugen Technologies, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nugen Technologies, Inc. filed Critical Nugen Technologies, Inc.
Priority to EP15836587.4A priority Critical patent/EP3186418A2/en
Publication of WO2016033251A2 publication Critical patent/WO2016033251A2/en
Publication of WO2016033251A3 publication Critical patent/WO2016033251A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups

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  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Provided herein are methods, compositions and kits for targeted nucleic acid sequence enrichment in a nucleic acid sample and for high efficiency nucleic acid library generation for next generation sequencing (NGS). The methods, compositions and kits provided herein can be useful for the production and capture of amplification-ready, target-specific and strand-specific regions of interest from nucleic acid samples containing complex DNA.
PCT/US2015/047053 2014-08-26 2015-08-26 Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation WO2016033251A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP15836587.4A EP3186418A2 (en) 2014-08-26 2015-08-26 Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201462042240P 2014-08-26 2014-08-26
US62/042,240 2014-08-26
US201562188337P 2015-07-02 2015-07-02
US62/188,337 2015-07-02

Publications (2)

Publication Number Publication Date
WO2016033251A2 WO2016033251A2 (en) 2016-03-03
WO2016033251A3 true WO2016033251A3 (en) 2016-04-21

Family

ID=55400819

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2015/047053 WO2016033251A2 (en) 2014-08-26 2015-08-26 Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation

Country Status (2)

Country Link
EP (1) EP3186418A2 (en)
WO (1) WO2016033251A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
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US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing

Families Citing this family (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2533882B (en) 2012-01-26 2016-10-12 Nugen Tech Inc Method of enriching and sequencing nucleic acids of interest using massively parallel sequencing
US20150011396A1 (en) 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
CN104769127A (en) 2012-08-14 2015-07-08 10X基因组学有限公司 Microcapsule compositions and methods
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10400280B2 (en) 2012-08-14 2019-09-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
AU2013359165B2 (en) 2012-12-14 2019-09-12 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
AU2014214682B2 (en) 2013-02-08 2018-07-26 10X Genomics, Inc. Polynucleotide barcode generation
WO2015157567A1 (en) 2014-04-10 2015-10-15 10X Genomics, Inc. Fluidic devices, systems, and methods for encapsulating and partitioning reagents, and applications of same
WO2015200869A1 (en) 2014-06-26 2015-12-30 10X Genomics, Inc. Analysis of nucleic acid sequences
JP6838969B2 (en) 2014-06-26 2021-03-03 １０エックス ジェノミクス， インコーポレイテッド Method for Analyzing Nucleic Acids Derived from Individual Cells or Cell Populations
CA2972969A1 (en) 2015-01-12 2016-07-21 10X Genomics, Inc. Processes and systems for preparing nucleic acid sequencing libraries and libraries prepared using same
WO2016137973A1 (en) 2015-02-24 2016-09-01 10X Genomics Inc Partition processing methods and systems
US10240196B2 (en) * 2016-05-27 2019-03-26 Agilent Technologies, Inc. Transposase-random priming DNA sample preparation
US20180016632A1 (en) * 2016-07-12 2018-01-18 Kapa Biosystems, Inc. System and method for transposase-mediated amplicon sequencing
CN110214186B (en) 2017-01-30 2023-11-24 10X基因组学有限公司 Methods and systems for droplet-based single cell barcoding
CN108949911B (en) 2017-05-25 2022-10-14 北京大学 Methods for the identification and quantification of low-frequency somatic mutations
US20180340169A1 (en) 2017-05-26 2018-11-29 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
CN116064732A (en) 2017-05-26 2023-05-05 10X基因组学有限公司 Single-cell analysis of transposase-accessible chromatin
CN107586835B (en) * 2017-10-19 2020-11-03 东南大学 Single-chain-linker-based construction method and application of next-generation sequencing library
WO2019099751A1 (en) 2017-11-15 2019-05-23 10X Genomics, Inc. Functionalized gel beads
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
EP3749779B1 (en) * 2018-02-07 2024-12-25 Tecan Genomics, Inc. Library preparation
WO2019195166A1 (en) 2018-04-06 2019-10-10 10X Genomics, Inc. Systems and methods for quality control in single cell processing
US12065688B2 (en) 2018-08-20 2024-08-20 10X Genomics, Inc. Compositions and methods for cellular processing
WO2020136440A2 (en) * 2018-12-28 2020-07-02 National University Of Singapore Methods for targeted complementary dna enrichment
CN113355750A (en) * 2021-01-06 2021-09-07 南京诺唯赞生物科技股份有限公司 rRNA silent RNA library construction method and kit

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013059740A1 (en) * 2011-10-21 2013-04-25 Foundation Medicine, Inc. Novel alk and ntrk1 fusion molecules and uses thereof
US20130231253A1 (en) * 2012-01-26 2013-09-05 Doug Amorese Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library regeneration

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013059740A1 (en) * 2011-10-21 2013-04-25 Foundation Medicine, Inc. Novel alk and ntrk1 fusion molecules and uses thereof
US20130231253A1 (en) * 2012-01-26 2013-09-05 Doug Amorese Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library regeneration

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing

Also Published As

Publication number Publication date
WO2016033251A2 (en) 2016-03-03
EP3186418A2 (en) 2017-07-05

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