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WO2013015589A3 - 나노클러스터 형성을 이용한 단백질간 상호작용 분석 방법 및 키트 - Google Patents

나노클러스터 형성을 이용한 단백질간 상호작용 분석 방법 및 키트 Download PDF

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Publication number
WO2013015589A3
WO2013015589A3 PCT/KR2012/005868 KR2012005868W WO2013015589A3 WO 2013015589 A3 WO2013015589 A3 WO 2013015589A3 KR 2012005868 W KR2012005868 W KR 2012005868W WO 2013015589 A3 WO2013015589 A3 WO 2013015589A3
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protein
kit
incorporates
polynucleotide
interaction analysis
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PCT/KR2012/005868
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French (fr)
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WO2013015589A2 (ko
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허원도
김선창
이상규
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한국과학기술원
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Priority to US14/234,243 priority Critical patent/US9784747B2/en
Publication of WO2013015589A2 publication Critical patent/WO2013015589A2/ko
Publication of WO2013015589A3 publication Critical patent/WO2013015589A3/ko

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    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6845—Methods of identifying protein-protein interactions in protein mixtures
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1055—Protein x Protein interaction, e.g. two hybrid selection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/60—Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/735—Fusion polypeptide containing domain for protein-protein interaction containing a domain for self-assembly, e.g. a viral coat protein (includes phage display)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/91—Transferases (2.)
    • G01N2333/912—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • G01N2333/91205—Phosphotransferases in general

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

본 발명은 단백질간의 상호작용의 효율적인 분석을 위해, 제1형광단백질 및 제1자기조립단백질을 포함하는 제1융합단백질을 암호화하고 프로모터에 작동가능하게 연결된 제1폴리뉴클레오티드, 및 미끼 단백질을 암호화하는 폴리뉴클레오티드가 상기 제1융합단백질을 암호화하는 폴리뉴클레오티드에 작동가능하도록 연결될 수 있는 다중클로닝부위를 포함하는 제1발현벡터; 및 제2형광단백질 및 제2자기조립단백질을 포함하는 제2융합단백질을 암호화하고 프로모터에 작동가능하게 연결된 제2폴리뉴클레오티드, 및 표적 단백질을 암호화하는 폴리뉴클레오티드가 상기 제2융합단백질을 암호화하는 폴리뉴클레오티드에 작동가능하도록 연결될 수 있는 다중클로닝부위를 포함하는 제2발현벡터를 포함하는 단백질간 상호작용 분석용 키트를 제공한다.
PCT/KR2012/005868 2011-07-22 2012-07-23 나노클러스터 형성을 이용한 단백질간 상호작용 분석 방법 및 키트 WO2013015589A2 (ko)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US14/234,243 US9784747B2 (en) 2011-07-22 2012-07-23 Method and kit for analyzing protein-protein interaction using nanocluster formation

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020110073067A KR101356787B1 (ko) 2011-07-22 2011-07-22 나노클러스터 형성을 이용한 단백질간 상호작용 분석 방법 및 키트
KR10-2011-0073067 2011-07-22

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WO2013015589A2 WO2013015589A2 (ko) 2013-01-31
WO2013015589A3 true WO2013015589A3 (ko) 2013-06-13

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US (1) US9784747B2 (ko)
KR (1) KR101356787B1 (ko)
WO (1) WO2013015589A2 (ko)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106164344B (zh) 2014-02-07 2018-12-18 韩国生命工学研究院 使用流式细胞仪测定蛋白纳米颗粒分子间相互作用的方法
US10865394B2 (en) 2015-07-27 2020-12-15 Washington University Toolkit for the production of post-translationally modified proteins

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020106632A1 (en) * 2001-01-04 2002-08-08 Myriad Genetics, Inc. Screen assay for selecting protein-protein interaction modulators
EP1645881A1 (en) * 2004-10-05 2006-04-12 DKFZ Deutsches Krebsforschungszentrum Screening process for the detection and characterization of protein-protein-interactions in vivo by fluorescence cross correlation spectroscopy
KR20090018585A (ko) * 2007-08-17 2009-02-20 한국과학기술원 물질의 상호작용 검출 방법
KR100886312B1 (ko) * 2006-01-20 2009-03-04 연세대학교 산학협력단 단백질-단백질의 상호작용을 분석하는 방법

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101190157B1 (ko) * 2010-05-17 2012-10-12 한국과학기술원 Ｒａｂ５를 이용한 세포내 단백질 상호 작용 시각화 방법

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020106632A1 (en) * 2001-01-04 2002-08-08 Myriad Genetics, Inc. Screen assay for selecting protein-protein interaction modulators
EP1645881A1 (en) * 2004-10-05 2006-04-12 DKFZ Deutsches Krebsforschungszentrum Screening process for the detection and characterization of protein-protein-interactions in vivo by fluorescence cross correlation spectroscopy
KR100886312B1 (ko) * 2006-01-20 2009-03-04 연세대학교 산학협력단 단백질-단백질의 상호작용을 분석하는 방법
KR20090018585A (ko) * 2007-08-17 2009-02-20 한국과학기술원 물질의 상호작용 검출 방법

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
KYOUNG HU LEE ET AL.: "Visualizing dynamic interaction between calmodulin and calmodulin-related kinases via a monitoring method in live mammalian cells", PNAS, vol. 107, no. 8, 23 February 2010 (2010-02-23), pages 3412 - 3417, XP055071082 *

Also Published As

Publication number Publication date
KR20130012043A (ko) 2013-01-31
US9784747B2 (en) 2017-10-10
WO2013015589A2 (ko) 2013-01-31
US20150044712A1 (en) 2015-02-12
KR101356787B1 (ko) 2014-02-06

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WO2013015589A3 (ko) 나노클러스터 형성을 이용한 단백질간 상호작용 분석 방법 및 키트

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