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WO2013009818A3 - Cétoacide réductoisomérases de haute performance - Google Patents

Cétoacide réductoisomérases de haute performance Download PDF

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Publication number
WO2013009818A3
WO2013009818A3 PCT/US2012/046185 US2012046185W WO2013009818A3 WO 2013009818 A3 WO2013009818 A3 WO 2013009818A3 US 2012046185 W US2012046185 W US 2012046185W WO 2013009818 A3 WO2013009818 A3 WO 2013009818A3
Authority
WO
WIPO (PCT)
Prior art keywords
seq
ketol
kari
performance
recombinant microorganisms
Prior art date
Application number
PCT/US2012/046185
Other languages
English (en)
Other versions
WO2013009818A2 (fr
Inventor
Peter Meinhold
Doug Lies
Stephanie Porter-Scheinman
Christopher Smith
Christopher Snow
Sabine Bastian
Sebastian Schoof
Original Assignee
Gevo, Inc.
California Institute Of Technology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US13/303,884 external-priority patent/US20120190089A1/en
Application filed by Gevo, Inc., California Institute Of Technology filed Critical Gevo, Inc.
Priority to US14/131,984 priority Critical patent/US20140295513A1/en
Publication of WO2013009818A2 publication Critical patent/WO2013009818A2/fr
Publication of WO2013009818A3 publication Critical patent/WO2013009818A3/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/16—Butanols
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/746—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for lactic acid bacteria (Streptococcus; Lactococcus; Lactobacillus; Pediococcus; Enterococcus; Leuconostoc; Propionibacterium; Bifidobacterium; Sporolactobacillus)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01086—Ketol-acid reductoisomerase (1.1.1.86)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

La présente invention a pour objet des microorganismes de recombinaison comprenant au moins une molécule d'acide nucléique codant une cétoacide réductoisomérase (KARI) ou son variant dépendant de NADH modifié, ladite KARI étant identique à au moins environ 80 % à SEQ ID NO : 2, SEQ ID NO : 4, SEQ ID NO : 6, SEQ ID NO : 8, SEQ ID NO : 10, SEQ ID NO : 28, SEQ ID NO : 40, ou SEQ ID NO : 58. La présente invention concerne également des microorganismes de recombinaison comprenant au moins une molécule d'acide nucléique codant une cétoacide réductoisomérase (KARI) ou son variant dépendant de NADH modifié, ladite KARI étant identique à au moins environ 99 % à SEQ ID NO : 64. Selon divers aspects de l'invention, les microorganismes de recombinaison peuvent comprendre une voie métabolique de production d'isobutanol et peuvent être utilisés dans des procédés de fabrication d'isobutanol.
PCT/US2012/046185 2011-07-11 2012-07-11 Cétoacide réductoisomérases de haute performance WO2013009818A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US14/131,984 US20140295513A1 (en) 2011-07-11 2012-07-11 High-Performance Ketol-Acid Reductoisomerases

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
US201161506564P 2011-07-11 2011-07-11
US201161506562P 2011-07-11 2011-07-11
US61/506,562 2011-07-11
US61/506,564 2011-07-11
US201161510618P 2011-07-22 2011-07-22
US61/510,618 2011-07-22
US13/303,884 US20120190089A1 (en) 2008-10-31 2011-11-23 Engineered microogranisms capable of producing target compounds under anaerobic conditions
US13/303,884 2011-11-23

Publications (2)

Publication Number Publication Date
WO2013009818A2 WO2013009818A2 (fr) 2013-01-17
WO2013009818A3 true WO2013009818A3 (fr) 2013-04-25

Family

ID=47506882

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2012/046185 WO2013009818A2 (fr) 2011-07-11 2012-07-11 Cétoacide réductoisomérases de haute performance

Country Status (1)

Country Link
WO (1) WO2013009818A2 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MA44605A (fr) * 2016-04-06 2019-02-13 Immatics Biotechnologies Gmbh Nouveaux peptides et combinaison de peptides pour une utilisation dans l'immunothérapie contre la leucémie myéloïde aiguë (lma) et d'autres cancers
CN113278655B (zh) * 2020-05-13 2022-05-17 安徽华恒生物科技股份有限公司 生产l-缬氨酸的重组微生物及构建方法、应用

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080148432A1 (en) * 2005-12-21 2008-06-19 Mark Scott Abad Transgenic plants with enhanced agronomic traits
US20100143997A1 (en) * 2008-10-31 2010-06-10 Thomas Buelter Engineered microorganisms capable of producing target compounds under anaerobic conditions
US20100197519A1 (en) * 2007-12-20 2010-08-05 E. I. Du Pont De Nemours And Company Ketol-acid reductoisomerase using nadh

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080148432A1 (en) * 2005-12-21 2008-06-19 Mark Scott Abad Transgenic plants with enhanced agronomic traits
US20100197519A1 (en) * 2007-12-20 2010-08-05 E. I. Du Pont De Nemours And Company Ketol-acid reductoisomerase using nadh
US20100143997A1 (en) * 2008-10-31 2010-06-10 Thomas Buelter Engineered microorganisms capable of producing target compounds under anaerobic conditions

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
DATABASE GENBANK 5 September 2006 (2006-09-05), COPELAND ET AL.: "ketol-acid reductoisomerase [Shewanella sp. MR-4]", accession no. P000446.1. *
WEGMANN ET AL.: "Complete Genome Sequence of the Prototype Lactic Acid Bacterium Lactococcus lactis subsp. Cremoris MG1363.", JOURNAL OF BACTERIOLOGY., vol. 189, no. 8, 16 February 2007 (2007-02-16), pages 3256 - 3270 *

Also Published As

Publication number Publication date
WO2013009818A2 (fr) 2013-01-17

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