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WO2013067069A3 - Dual digestion method for high yield nucleic acid recovery - Google Patents

Dual digestion method for high yield nucleic acid recovery Download PDF

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Publication number
WO2013067069A3
WO2013067069A3 PCT/US2012/062884 US2012062884W WO2013067069A3 WO 2013067069 A3 WO2013067069 A3 WO 2013067069A3 US 2012062884 W US2012062884 W US 2012062884W WO 2013067069 A3 WO2013067069 A3 WO 2013067069A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
high yield
acid recovery
digestion method
yield nucleic
Prior art date
Application number
PCT/US2012/062884
Other languages
French (fr)
Other versions
WO2013067069A2 (en
Inventor
Barbara LEINWEBER
Paul DIGGINS
Original Assignee
Leinweber Barbara
Diggins Paul
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Leinweber Barbara, Diggins Paul filed Critical Leinweber Barbara
Publication of WO2013067069A2 publication Critical patent/WO2013067069A2/en
Publication of WO2013067069A3 publication Critical patent/WO2013067069A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Methods and kits for isolating nucleic acids from a sample are provided, comprising performing digestion of a biological sample under acidic and reduced temperature conditions, and optionally comprising performing prior digestion under alkaline conditions.
PCT/US2012/062884 2011-10-31 2012-10-31 Dual digestion method for high yield nucleic acid recovery WO2013067069A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161553842P 2011-10-31 2011-10-31
US61/553,842 2011-10-31

Publications (2)

Publication Number Publication Date
WO2013067069A2 WO2013067069A2 (en) 2013-05-10
WO2013067069A3 true WO2013067069A3 (en) 2015-06-11

Family

ID=48193024

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2012/062884 WO2013067069A2 (en) 2011-10-31 2012-10-31 Dual digestion method for high yield nucleic acid recovery

Country Status (1)

Country Link
WO (1) WO2013067069A2 (en)

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5386024A (en) * 1993-02-10 1995-01-31 Gen-Probe Incorporated Method to prepare nucleic acids from a biological sample using low pH and acid protease
US5981235A (en) * 1996-07-29 1999-11-09 Promega Corporation Methods for isolating nucleic acids using alkaline protease
US20040157244A1 (en) * 2002-12-23 2004-08-12 Vical Incorporated Process for purification of plasmid DNA
US20070160999A1 (en) * 2004-02-04 2007-07-12 Fiorella Calabrese Method for simultaneous extraction of nucleic acids from a biological sample
US20090182120A1 (en) * 2005-01-21 2009-07-16 Argylla Technologies, Llp Surface mediated self-assembly of nanoparticles

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5386024A (en) * 1993-02-10 1995-01-31 Gen-Probe Incorporated Method to prepare nucleic acids from a biological sample using low pH and acid protease
US5981235A (en) * 1996-07-29 1999-11-09 Promega Corporation Methods for isolating nucleic acids using alkaline protease
US20040157244A1 (en) * 2002-12-23 2004-08-12 Vical Incorporated Process for purification of plasmid DNA
US20070160999A1 (en) * 2004-02-04 2007-07-12 Fiorella Calabrese Method for simultaneous extraction of nucleic acids from a biological sample
US20090182120A1 (en) * 2005-01-21 2009-07-16 Argylla Technologies, Llp Surface mediated self-assembly of nanoparticles

Also Published As

Publication number Publication date
WO2013067069A2 (en) 2013-05-10

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