WO2013053381A1 - Procédé et dispositif servant à déterminer une concentration de matière par spectroscopie de fluorescence - Google Patents
Procédé et dispositif servant à déterminer une concentration de matière par spectroscopie de fluorescence Download PDFInfo
- Publication number
- WO2013053381A1 WO2013053381A1 PCT/EP2011/067718 EP2011067718W WO2013053381A1 WO 2013053381 A1 WO2013053381 A1 WO 2013053381A1 EP 2011067718 W EP2011067718 W EP 2011067718W WO 2013053381 A1 WO2013053381 A1 WO 2013053381A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- radiation
- sample
- detector
- modulation frequency
- phase position
- Prior art date
Links
- 239000000126 substance Substances 0.000 title claims abstract description 54
- 238000000034 method Methods 0.000 title claims abstract description 19
- 238000001506 fluorescence spectroscopy Methods 0.000 title claims abstract description 9
- 230000005855 radiation Effects 0.000 claims abstract description 198
- 230000005670 electromagnetic radiation Effects 0.000 claims abstract description 21
- 238000011156 evaluation Methods 0.000 claims description 40
- 238000001228 spectrum Methods 0.000 claims description 16
- 230000003287 optical effect Effects 0.000 claims description 13
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 9
- 229910052760 oxygen Inorganic materials 0.000 claims description 9
- 239000001301 oxygen Substances 0.000 claims description 9
- 230000008859 change Effects 0.000 claims description 7
- 230000001419 dependent effect Effects 0.000 claims description 7
- 239000011521 glass Substances 0.000 claims description 4
- 239000010979 ruby Substances 0.000 claims description 4
- 229910001750 ruby Inorganic materials 0.000 claims description 4
- 230000003993 interaction Effects 0.000 claims description 3
- 230000002452 interceptive effect Effects 0.000 claims 1
- 230000010363 phase shift Effects 0.000 description 16
- 238000011161 development Methods 0.000 description 9
- 230000018109 developmental process Effects 0.000 description 9
- 239000000463 material Substances 0.000 description 5
- 238000005259 measurement Methods 0.000 description 5
- 230000000875 corresponding effect Effects 0.000 description 4
- 238000013459 approach Methods 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 230000005540 biological transmission Effects 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 230000005284 excitation Effects 0.000 description 3
- 230000004044 response Effects 0.000 description 3
- 230000001360 synchronised effect Effects 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 2
- 230000004888 barrier function Effects 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 230000008030 elimination Effects 0.000 description 2
- 238000003379 elimination reaction Methods 0.000 description 2
- 230000001795 light effect Effects 0.000 description 2
- 238000004020 luminiscence type Methods 0.000 description 2
- 239000013307 optical fiber Substances 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 239000000853 adhesive Substances 0.000 description 1
- 230000001070 adhesive effect Effects 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 239000000945 filler Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 238000005558 fluorometry Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000000704 physical effect Effects 0.000 description 1
- 238000010791 quenching Methods 0.000 description 1
- 230000000171 quenching effect Effects 0.000 description 1
- 239000012925 reference material Substances 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- 238000004611 spectroscopical analysis Methods 0.000 description 1
- 230000002277 temperature effect Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6408—Fluorescence; Phosphorescence with measurement of decay time, time resolved fluorescence
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/27—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands using photo-electric detection ; circuits for computing concentration
- G01N21/274—Calibration, base line adjustment, drift correction
Definitions
- the first reference phase position results at a known substance concentration in the sample, preferably in the absence of the substance in the sample, and can be obtained, in particular, by dividing said first radiation into at least two portions before striking the sample by means of a beam splitter one of which is directed to the sample and the other essentially directly to the detector. If one now uses the phase shift of the signal with the first modulation frequency and the signal with the second modulation frequency, the evaluation according to the method described by Jameson et al. proposes proposed solution, it is possible to eliminate the effects of stray light and autofluorescence of the sample within certain limits evaluation technology.
- a “beam splitter” is understood to mean any optical element which is suitable for dividing the first radiation into at least two components, namely a (preferably comparatively small) component, in particular scattered component, which passes directly to the detector as reference radiation, and one
- the beam splitter serves at least partially to redirect the fluorescence radiation emitted by the specimen in the direction of the detector, which advantageously can be linked to a filter function so that only certain, preferably relatively, ones are associated
- a dichroic mirror may be used in this context (see below), but the invention would not be restricted to such beam headers
- First radiation essentially in that due to scattered light effects of the overall arrangement, in particular so the mirror environment, a small proportion of radiation passes directly to the detector.
- At least one first radiation source which is designed to emit at least one first electromagnetic radiation, preferably in the visible region of the spectrum;
- At least one first modulation means adapted to modulate the intensity of the first radiation having at least a first and a second modulation frequency which are different;
- At least one beam splitter adapted and arranged to direct a first portion of the first radiation to the sample and a second portion of the first radiation to the detector;
- the reference object is generally chosen such that it shows substantially no change in its fluorescence behavior as a function of the substance concentration to be determined, but a change in its fluorescence behavior as a function of the temperature occurs.
- the invention is limited in this context to the use of ruby as a reference object.
- the evaluation electronics are designed to evaluate the first or third radiation detected by the detector with the fourth modulation frequency with respect to its phase position relative to a third reference phase position in order additionally to optically determine a temperature of the sample.
- At least the second radiation source, the sample, the detector and optionally the filter element are arranged on a common side of the beam dispenser, preferably within the housing, whereby a particularly achieve compact design and a correspondingly flexible usability of the device.
- FIG. 2 shows schematically another embodiment of the device according to the invention, which is suitable for carrying out an alternative embodiment of the method according to the invention.
- FIG. 1 schematically shows a device for determining a substance concentration, preferably an oxygen concentration by means of fluorescence spectroscopy, which device is denoted by the reference numeral 1 in its entirety.
- the device 1 comprises at reference 2 a first radiation source in the form of a green luminescence diode (LED) with a wavelength of
Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Pathology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Engineering & Computer Science (AREA)
- Mathematical Physics (AREA)
- Theoretical Computer Science (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
L'invention concerne un procédé et un dispositif servant à déterminer une concentration de matière par spectroscopie de fluorescence, au moins un premier faisceau électromagnétique (S1) venant frapper un échantillon (5), l'intensité dudit faisceau étant modulée avec au moins deux fréquences de modulation (f1, f2) différentes et un faisceau fluorescent (S1') émis par l'échantillon (5) étant déterminé au moyen d'un détecteur (6) et évalué pour chaque fréquence de modulation en ce qui concerne sa position de phase par rapport à une première position de phase de référence respective, qui découle d'une concentration de matière connue dans l'échantillon (5), de préférence lorsque la matière est absente dans l'échantillon. Au moins un deuxième faisceau électromagnétique vient frapper le détecteur (6), faisceau dont l'intensité est modulée par au moins une troisième fréquence de modulation (f3) différente des première et deuxième fréquences de modulation. Le deuxième faisceau (S2) n'interagit pas avec l'échantillon (5) et est évalué, en atteignant le détecteur (6), en ce qui concerne sa position de phase par rapport à une deuxième position de phase de référence afin de déterminer la concentration de matière dans l'échantillon (5) sans tenir compte des changements d'état.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/EP2011/067718 WO2013053381A1 (fr) | 2011-10-11 | 2011-10-11 | Procédé et dispositif servant à déterminer une concentration de matière par spectroscopie de fluorescence |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/EP2011/067718 WO2013053381A1 (fr) | 2011-10-11 | 2011-10-11 | Procédé et dispositif servant à déterminer une concentration de matière par spectroscopie de fluorescence |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| WO2013053381A1 true WO2013053381A1 (fr) | 2013-04-18 |
Family
ID=44773087
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/EP2011/067718 WO2013053381A1 (fr) | 2011-10-11 | 2011-10-11 | Procédé et dispositif servant à déterminer une concentration de matière par spectroscopie de fluorescence |
Country Status (1)
| Country | Link |
|---|---|
| WO (1) | WO2013053381A1 (fr) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2887054A1 (fr) * | 2013-12-19 | 2015-06-24 | Mettler-Toledo AG | Procédé de mesure pour un capteur optochimique |
| EP4056990A1 (fr) * | 2021-03-08 | 2022-09-14 | Biomimetics Technologies Inc. | Procédé et dispositif de détermination respirométrique de l'efficacité antibacterienne des substances antibiotiques au moyen de l'oxymétrie à base luminescente |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5498549A (en) * | 1991-08-08 | 1996-03-12 | Minnesota Mining And Manufacturing Company | Sensors and methods for sensing |
| WO2005033746A2 (fr) * | 2003-09-29 | 2005-04-14 | Photosense L.L.C. | Instruments mesurant la luminescence dans le domaine des frequences |
| US20110095204A1 (en) * | 2004-07-27 | 2011-04-28 | Precisense A/S | Method and apparatus for measuring the phase shift iduced in a light signal by a sample |
-
2011
- 2011-10-11 WO PCT/EP2011/067718 patent/WO2013053381A1/fr active Application Filing
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5498549A (en) * | 1991-08-08 | 1996-03-12 | Minnesota Mining And Manufacturing Company | Sensors and methods for sensing |
| WO2005033746A2 (fr) * | 2003-09-29 | 2005-04-14 | Photosense L.L.C. | Instruments mesurant la luminescence dans le domaine des frequences |
| US20110095204A1 (en) * | 2004-07-27 | 2011-04-28 | Precisense A/S | Method and apparatus for measuring the phase shift iduced in a light signal by a sample |
Non-Patent Citations (2)
| Title |
|---|
| "Principles of Fluorescence Spectroscopy", 2006, SPRINGER |
| VON JAMESON ET AL.: "The Measurement and Analysis of Heterogeneous Emissions by Multifrequency Phase and Modulation Fluorometry", APPLIED SPECTROSCOPY REVIEWS, vol. 20, no. 1, 1984, pages 55 - 106, XP009076450 |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2887054A1 (fr) * | 2013-12-19 | 2015-06-24 | Mettler-Toledo AG | Procédé de mesure pour un capteur optochimique |
| EP4056990A1 (fr) * | 2021-03-08 | 2022-09-14 | Biomimetics Technologies Inc. | Procédé et dispositif de détermination respirométrique de l'efficacité antibacterienne des substances antibiotiques au moyen de l'oxymétrie à base luminescente |
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