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WO2012013366A2 - Vernis bactéricide préventif pour le traitement des ongles - Google Patents

Vernis bactéricide préventif pour le traitement des ongles Download PDF

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Publication number
WO2012013366A2
WO2012013366A2 PCT/EP2011/055666 EP2011055666W WO2012013366A2 WO 2012013366 A2 WO2012013366 A2 WO 2012013366A2 EP 2011055666 W EP2011055666 W EP 2011055666W WO 2012013366 A2 WO2012013366 A2 WO 2012013366A2
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WIPO (PCT)
Prior art keywords
bactericidal
polish
treatment
bentonite
preventive
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PCT/EP2011/055666
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English (en)
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WO2012013366A3 (fr
Inventor
Ara Arshavirovich Abramyan
Viacheslav Ivanovich Beklemyshev
Konstantin Vitalievich Filippov
Umberto Orazio Giuseppe Maugeri
Vladimir Aleksandrovich Solodovnikov
Original Assignee
Closed Stock Company "Institute Of Applied Nanotechnology"
Fondazione Salvatore Maugeri Clinica Del Lavoro E Della Riabilitazione
Sib Laboratories Limited
Makhonin, Igor Ivanovich
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Application filed by Closed Stock Company "Institute Of Applied Nanotechnology", Fondazione Salvatore Maugeri Clinica Del Lavoro E Della Riabilitazione, Sib Laboratories Limited, Makhonin, Igor Ivanovich filed Critical Closed Stock Company "Institute Of Applied Nanotechnology"
Publication of WO2012013366A2 publication Critical patent/WO2012013366A2/fr
Publication of WO2012013366A3 publication Critical patent/WO2012013366A3/fr

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K33/00—Medicinal preparations containing inorganic active ingredients
    • A61K33/24—Heavy metals; Compounds thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/0012—Galenical forms characterised by the site of application
    • A61K9/0014—Skin, i.e. galenical aspects of topical compositions
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
    • A61K31/716—Glucans
    • A61K31/722—Chitin, chitosan
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K33/00—Medicinal preparations containing inorganic active ingredients
    • A61K33/06—Aluminium, calcium or magnesium; Compounds thereof, e.g. clay
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K33/00—Medicinal preparations containing inorganic active ingredients
    • A61K33/24—Heavy metals; Compounds thereof
    • A61K33/30—Zinc; Compounds thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K33/00—Medicinal preparations containing inorganic active ingredients
    • A61K33/24—Heavy metals; Compounds thereof
    • A61K33/34—Copper; Compounds thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/02—Inorganic compounds
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
    • A61K8/0241—Containing particulates characterized by their shape and/or structure
    • A61K8/0254—Platelets; Flakes
    • A61K8/0258—Layered structure
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/19—Cosmetics or similar toiletry preparations characterised by the composition containing inorganic ingredients
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/19—Cosmetics or similar toiletry preparations characterised by the composition containing inorganic ingredients
    • A61K8/26—Aluminium; Compounds thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/19—Cosmetics or similar toiletry preparations characterised by the composition containing inorganic ingredients
    • A61K8/27—Zinc; Compounds thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
    • A61K8/73—Polysaccharides
    • A61K8/736—Chitin; Chitosan; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents
    • A61P31/06—Antibacterial agents for tuberculosis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/10—Antimycotics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q3/00—Manicure or pedicure preparations
    • A61Q3/02—Nail coatings
    • C—CHEMISTRY; METALLURGY
    • C01—INORGANIC CHEMISTRY
    • C01B—NON-METALLIC ELEMENTS; COMPOUNDS THEREOF; METALLOIDS OR COMPOUNDS THEREOF NOT COVERED BY SUBCLASS C01C
    • C01B33/00—Silicon; Compounds thereof
    • C01B33/20—Silicates
    • C01B33/36—Silicates having base-exchange properties but not having molecular sieve properties
    • C01B33/38—Layered base-exchange silicates, e.g. clays, micas or alkali metal silicates of kenyaite or magadiite type
    • C01B33/40—Clays
    • C—CHEMISTRY; METALLURGY
    • C01—INORGANIC CHEMISTRY
    • C01B—NON-METALLIC ELEMENTS; COMPOUNDS THEREOF; METALLOIDS OR COMPOUNDS THEREOF NOT COVERED BY SUBCLASS C01C
    • C01B33/00—Silicon; Compounds thereof
    • C01B33/20—Silicates
    • C01B33/36—Silicates having base-exchange properties but not having molecular sieve properties
    • C01B33/38—Layered base-exchange silicates, e.g. clays, micas or alkali metal silicates of kenyaite or magadiite type
    • C01B33/44—Products obtained from layered base-exchange silicates by ion-exchange with organic compounds such as ammonium, phosphonium or sulfonium compounds or by intercalation of organic compounds, e.g. organoclay material
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
    • A61K2800/41—Particular ingredients further characterized by their size
    • A61K2800/413—Nanosized, i.e. having sizes below 100 nm
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
    • A61K2800/56—Compounds, absorbed onto or entrapped into a solid carrier, e.g. encapsulated perfumes, inclusion compounds, sustained release forms
    • C—CHEMISTRY; METALLURGY
    • C01—INORGANIC CHEMISTRY
    • C01P—INDEXING SCHEME RELATING TO STRUCTURAL AND PHYSICAL ASPECTS OF SOLID INORGANIC COMPOUNDS
    • C01P2004/00—Particle morphology
    • C01P2004/60—Particles characterised by their size
    • C01P2004/62—Submicrometer sized, i.e. from 0.1-1 micrometer

Definitions

  • the invention concerns the area of medicine, hygiene and cosmetology and, specifically, a preventive bactericidal enamel for nail treatment.
  • onichyte mycosis Mycosis of nail and nail cushion (onichyte mycosis) is a widespread illness which cannot be well cured in many cases.
  • the main activators of onichyte mycosis are dermatophytes, funguses capable to invade keratin and attack skin, nails and hair. Funguses of kind Trichophyton, Epidermophyton, Microsporum are among them.
  • Funguses of kind Trichophyton, Epidermophyton, Microsporum are among them.
  • dermatophytes superficial mycosises can be caused by others funguses, for example, Candida spp.
  • Candida spp. are frequently detected after dermatophytes at epidermomycosis and onichyte mycosis; these funguses cause a candidiasis of nails.
  • Loss of transparency, change of color (whitish, yellowish), thickening, hyponychial hyperkeratosis, crumbling or destruction up to the nail platen are characteristic symptoms for various types of fungous affections of nail plates (onichyte mycosis).
  • bactericidal polishes are more favourable in comparison with other ways of treatment and preventive protection, as it does not possess side effects and influences only locally the nails.
  • Various bactericidal polishes for treatment of nails and nail platens are known.
  • a bactericidal polish for treatment of onichyte mycosis of nails which contains effective amounts of tsiklopiroks and its pharmaceutically acceptable salts.
  • the polish contains a film-forming polymer which protects a nail exposed to treatment by means of formation of a solid, transparent and waterproof film.
  • an antifungal nail polish is described containing l-hydroxy-2-pyridone, for example tsiklopiroksolamin, a film-forming substance and physiologically compatible solvent and also usual additives for cosmetics.
  • compositions of polishes as antifungal agents are also used in compositions of polishes as antifungal agents:
  • - imidazole derivatives and their salts for example, clotrimazole, econazole, isoconazole, ketoconazole, miconazole, tioconazole, bifonazole, fenticonazole and oxiconazole;
  • the composition of the polish contains also organically modified bentonite on the basis of bentonite stearylalcony in amount up to 2% b.w. for improvement of its characteristics.
  • a bactericidal polish containing a film-forming substance, a bactericidal agent and a solvent is also known.
  • Bacillus subtilis or Bacillus licheniformis or their mix are used as a bactericidal agent.
  • the given polish contains also a preservative with antifungal properties on the basis of paraform.
  • the technology of obtaining the named bactericidal agent includes suspension cultivation of a strain of bacteria in a nutrient medium, separation of bacterial culture from the nutrient medium and then centrifugation for increase of concentration of bacteria.
  • the complex technology for obtaining the given agent leads to increase in expenses for manufacturing the polish.
  • the difficulties derive from the process of polymerization of formaldehyde for obtaining paraform (paraformaldehyde, polyoximethylene).
  • the preservative used is a derivative of formaldehyde which is the most cancerogenic, neurotoxic and allergenic among all preservatives.
  • composition of the polish described in said invention contains as the bactericidal agent:
  • antifungal substance or a combination of antifungal substances and, preferably, compounds of l-hydroxy-2-pyridones and their salts;
  • antibiotics for example, antibiotics, anti-inflammatory means, antiseptics and/or local anesthetics.
  • a film-forming substance on the basis of water-soluble derivatives of chitosan basically hydroxypropylchitosans and carboxyalkylchitosans
  • chitosan basically hydroxypropylchitosans and carboxyalkylchitosans
  • Use of a film-forming substance on the basis of water-soluble derivatives of chitosan, basically hydroxypropylchitosans and carboxyalkylchitosans in the known invention provides formation of a thin layer of a film permeable to moisture and air on the treated nail. That is suitable for use of the given polish for preventive purposes.
  • the presence of the specified film-forming substance in a composition of polish provides use of polar solvents, basically aqueous-alcoholic solutions, what avoids influence of aggressive organic solvents on nails and skin. As a result dermatological risks are reduced both at drawing and at removal of the polish film from the surface of the nail.
  • bactericidal activity of the given polish used for treatment of nails depends both on the presence of antifungal agents in its composition and on the presence of other active components, including antiseptic and anti-inflammatory ones.
  • the compounding of the known polish becomes complicated due to the insertion of additional active components. That increases costs for its manufacturing, also due to the use of active components on the basis of synthesized organic compounds having also a limited spectrum of action, in respect to various microbes and funguses.
  • Bactericidal agents on the basis of nano structured powders of bentonite, intercalated by ions of metals Ag + or/and Cu 2+ , are described in said patent. They are obtained by modifying a bentonite with solutions of inorganic salts of silver or copper. Bentonite in Na-form is preliminarly enriched with cations of sodium by processing with a water solution of an inorganic salt of sodium.
  • Bentonite obtained at the first stage of processing is cleaned from chlorine anions and bentonite obtained at the second stage, after intercalation by ions of the specified metals, is cleaned from salts of sodium. Nanostructuring of the product is carried out till a powder of bentonite with a size of the particles of no more than 150 nm is obtained.
  • the bactericidal agent is obtained from mineral, ecologically safe components, and provides efficiency of action concerning a wide spectrum of bacteria and funguses; it is safe dermatologically and can be used as additive to various preparations for medical and cosmetic purposes.
  • compositions of a bactericidal polish on the basis of the described bactericidal agent, for treatment of nails, is not foreseen in the given patent.
  • the problem of the present invention consisted in creating a bactericidal polish with preventing activity in treatment of nails, providing the technical result of expanding assortment of polishes corresponding to the technology requirements, decreasing the risks of occurrence of negative dermatological effects and providing effective bactericidal protection of treated nails while decreasing the expenses for manufacturing the polish composition.
  • a preventive bactericidal polish for treatment of nails containing a water-soluble film-forming substance, a bactericidal agent and a polar solvent.
  • powders of bentonite nano structured till the size of particles of no more than 150 nm and intercalated by ions of metals Ag + or/and Cu 2+ and Zn 2+ are used as the bactericidal agent.
  • the bactericide is introduced in the preliminarily prepared emulsion of the film-forming substance, as 4 - 10 % hydrosol.
  • a composition of the polish has the following content of components, expressed as percentage by weight (% b.w.):
  • nanostructured powders of bentonite, intercalated by ions of metals Ag + and Zn 2+ are used as the bactericidal agent at a ratio in the mix of 1: (0,5 ⁇ 1 ) parts by weight.
  • nanostructured powders of bentonite intercalated by ions of metals Cu 2+ and Zn 2+ are used as the bactericidal agent at a ratio in the mix of (1 ⁇ 0,5): (0,5 ⁇ 1) parts by weight.
  • nanostructured powders of bentonite intercalated by ions of metals Ag + , Cu 2+ and Zn 2+ are used as the bactericidal agent at a ratio in the mix of 1: (0,5): (0 ⁇ 1) parts by weight.
  • nanostructured powders of bentonite intercalated by ions of metals Ag + , Cu 2+ and Zn 2+ are used as the bactericidal agent at a ratio in the mix of 1: (0, 5): (0,5) parts by weight.
  • step b) nanostructuring the products obtained at the step b) by grinding them up to a size of the particles of no more than 150 nm with subsequent drying.
  • silver nitrate, copper sulfate, zinc sulfate or zinc chloride are used as 10-20 % water solutions of inorganic salts of silver, copper and zinc.
  • derivatives of chitosan preferably hydroxypropylchitosan or carboxyalkylchitosan, in amount of 3-5% b.w., are used as the water-soluble film-forming substance.
  • the nail composition additionally contains from 1 up to 5% b.w. of industrially suitable additives, preferably surfactants, lanolin derivatives, stabilizers at light, odors.
  • industrially suitable additives preferably surfactants, lanolin derivatives, stabilizers at light, odors.
  • a bactericidal agent on the basis of nano structured powders of bentonite with a size of particles of no more than 150 nm, intercalated by ions of metals Ag + , Cu 2+ ' Zn 2+ in the composite structure of a polish. That increases the effective area of interaction with the bacterial environment providing activity on pathogenic microflora concerning a wide spectrum of gram-positive, gram-negative bacteria and fungoid microorganisms;
  • a nanostructured mineral component i.e. bentonite with a size of the particles of no more than 150 nm with flow properties allowing improvement of structuring a composition of a polish creating a new composite structure of a polish
  • a film-forming substance compatible with polar solvents that provides the possibility of removal of a polish film from nails without use of the organic solvents adversely influencing the skin
  • the declared invention can be industrially realized for manufacturing preventive bactericidal polishes for treatment of nails.
  • Table 1- illustrates «Bactericidal properties of polish compositions*
  • Table 2 - illustrates «Fungicidal properties of polish compositions*.
  • Hydroxypropylchitosan and carboxyalkylchotosan are preferably used according to the present invention.
  • Hydroxypropylchitosans are derivatives of chitosans with hydroxy- groups.
  • Carboxyalkylchotosans are derivatives of chitosan with carboxy- groups.
  • the named water-soluble film-forming substances are used in amount from 1,0 up to 10,0% b.w. on the total mass of a composition of a polish; they provide effective formation of a film on the surface of a nail.
  • Decrease in the content of the specified film-forming substances in the composition of the polish reduces hiding power of the work surface of a nail and decreases technical time of protection of nails with preventive treatment.
  • the increase in the quantitative content of film-forming substance in the composition of the polish increases the charge of the given component and worsens quality of the formed film.
  • the bactericidal agent possessing antimicrobic and antifungal properties described in the patent RU No. 2330673 was used for realization of the present invention.
  • Bentonite (montmorillonite) in Na-form was saturated with 5% water solution of NaCl. There was at time interval of retention of bentonite in the given solution what caused enrichment of bentonite with ions of sodium. Then repeated washing for removal of anions of chlorine, with subsequent filtration and drying, was carried out.
  • Nanostructured bentonite powders (nanoparticles of bentonite), intercalated by ions of the specified metals and not containing salts of sodium, are obtained from semifinished products of bentonite made at the 1 st step, according to the following examples:
  • a semifinished product cleaned from acid anions was dried up and modified by 10- 20 % water solution of silver nitrate (at red illumination). 15 % water solution of silver nitrate (AgN03) was preferably used.
  • the obtained modified semifinished product was repeatedly washed out for removal of salts of sodium, filtered, grinded and dried.
  • the amount of water solution for processing 5 g of semifinished product (1 st step) was the following: bentonite : water solution as 1:20 parts by weight.
  • Example 2 The same materials and technical methods as in the Example 1 were used, but 15% water solution of copper sulfate (CuS04) was used to modify the bentonite enriched with ions of sodium Na + .
  • CuS04 copper sulfate
  • Example 2 The same materials and technical methods as in the Example 1 were used, but 15% water solution of an inorganic salt of zinc, preferably zinc chloride (ZnCl 2 ), was used to modify bentonite enriched with ions of sodium Na + . It is possible to use zinc sulfate (ZnS0 4 ).
  • ZnCl 2 zinc chloride
  • ZnS0 4 zinc sulfate
  • Deionized water is used for realization of technical steps 1 and 2 mentioned above.
  • Nanostructuring (superdispersion) up to the size of particles of no more than 150 nm was carried out with the help of an ultrasonic dispersant, accelerating nanostructuring of the bentonite powders.
  • the colloidal systems obtained were powered on a sublayer and, after drying, the sizes of the particles of the nanostructured powder of bentonite were measured by methods of microscopy. The tests performed have shown that nanostructured powders of bentonite have sizes of the particles of no more than 150 nm.
  • Nanostructured powders of bentonite (Examples 1-3) were analyzed to define the content of silver, copper and zinc (in % b.w.) with the method of titrimetric analysis.
  • the titrimetric analysis was carried out by using indicators, fixing a point of equivalence of titration.
  • powders of bentonite intercalated by ions of the named metals, can contain from 2,0 up to 8,0% b.w. of silver or copper or zinc, depending on the amount of activating and modifying (intercalating) reagents.
  • the specified contents of metals in an intercalated (modified) bentonite powder are the optimum. Increase of the percentage of the contents of the metals leads to increase in costs for the reagents used for obtaining bentonite powders. Decrease of the percentage of the contents of the named metals in bentonite powders leads to a decrease of their antimicrobic and fungicidal activity. According to the results of the titrimetric analyses the quantitative contents of metals (Ag, Cu, Zn) in nano structured powders of bentonite (products of Examples 1-3) was about 2,0-8,0% b.w..
  • Bactericidal agents used according to the present invention have been obtained by mixing products according to the Examples 1-3, in a ratio in parts b.w. as hereinafter specified.
  • a bactericidal agent containing nanostructured powders of bentonite, intercalated by ions of metals Ag + and Zn 2+ (Example 1 and Example 3), at a ratio in the mix as 1: 0,7
  • a bactericidal agent containing nanostructured powders of bentonite, intercalated by ions of metals Ag + and Cu 2+ (Example 1 and Example 2), at a ratio in the mix as 1: (0,5).
  • the amount of the bactericidal agents specified in the present invention, when included in a composition of a polish, is the optimum for maintaining antimicrobic and antifungal protection of nails and integuments adjoining to them, if used as a preventive treatment.
  • the increase in amount of the bactericidal agent in the composition of a polish leads to increase in costs and to deterioration of the structure of the polish composition, resulting in formation of unaesthetic polish films on the surface of nails.
  • the ratio of mixes of nano structured powders of bentonite intercalated by ions of metals Ag + , Cu 2+ and Zn 2+ specified in the present invention and used in the bactericidal agent is the optimum for preparing a polish composition intended for preventive protection of nails from a wide spectrum of gram-positive and gram-negative bacteria and fungoid microorganisms.
  • a polar solvent preferably 30-50 % water-alcohol solution, is used in compounding a polish composition according to the present invention.
  • Ethanol or isopropyl alcohol are used as the alcohol.
  • the presence of an alcohol in the polish provides a time of drying of the formed film of no more than 10 minutes, when a polish composition according to the invention is used. What corresponds to the consumer requirements for the given type of products (GOST R 52701-2006).
  • Additives which are traditional for the given kind of products, can be used to improve the properties required by the consumers for a polish composition according to the present invention.
  • various scents for example derivatives of lanolin, surfactants, stabilizers to light, etc.
  • the bactericidal agents obtained in Examples 4-7 as 4-10% hydrosols, preferably 5% hydrosols, have been added in preliminarily prepared water-alcohol emulsions of the film-forming substance. Hydroxypropylchitosan is used as the film-forming substance.
  • Use of hydrosols, for adding in the emulsion of the film-forming substance, provides uniformity of distribution of the bactericidal agent in said film-forming substance.
  • the amount of the bactericidal agent in a polar solvent, specified in the present invention, is the optimum for formation of a stable dispersed system of hydrosols. Decrease or increase in % b.w. of the contents of a bentonite powder in the polar solvent worsens stability, self-organizing of dispersed systems and uniformity of distribution of hydrosols in the emulsion of the film-forming substance.
  • the sizes of the particles of a bentonite powder specified in the invention are optimum both for costs for their obtaining and for stability of the obtained hydrosol.
  • Polishing compositions according to the following examples have been prepared: Example 1.4.
  • Hydroxypropylchitosan - 5% b.w.; bactericidal agent (Example 4) - 0,2% b.w.; 40% water-alcohol solution - the rest. In total -100 g.
  • Hydroxypropylchitosan - 5% b.w.; bactericidal agent (Example 6) - 1,0% b.w.; 40% water-alcohol solution - the rest. In total -100 g.
  • Carboxyalkylchitosan - 3% b.w.; bactericidal agent (Example 7) - 1,5% b.w.;
  • the disco-diffusion method (DDM) of definition of sensitivity of microorganisms is based on ability of the bactericidal agents, included in polish compositions, to diffuse in nutrient mediums from the cardboard (paper) disks impregnated with them thus stopping growth of the microorganisms sown on a surface of nutrient mediums.
  • a dense nutrient medium i.e. Trypcase-soy agar, was used for the estimation of the bactericidal activity of polish compositions.
  • a dense nutrient medium i.e. Sabouraud aga and Czapek-Dox agar, was used for estimation of the antifungal activity of the polish compositions.
  • Staphylococcus aureus Bacteria of the kind Staphylococcus aureus are the most resistant representatives of gram-positive microflora of a human being. They are responsible for pustular infections of skin, including furuncles and abscesses.
  • Bacteria of kind Pseudomonas aeruginosa are one of the most resistant representatives of gram-negative microflora. They possess high resistance to physical and chemical factors. They often display resistance to many drugs and disinfectants. Bacteria of this kind are known as activators of infectious complications of wounds from burning, bacteremias, septicemias with a fatal outcome and other complications of infectious etiology.
  • Funguses of kind Trichophyton are dermatophytes and are also one of the basic activators of superficial mycosis (epidermomycosis) on nails, skin and hair.
  • Funguses of the kind Candida spp. are the second, after dermatophytes, for frequency of release at epidermomycosis and onychomycosis.
  • Candida albicans cause a candidiasis of nails.
  • Suspensions (inoculums) of each test-culture of bacteria and test-culture of funguses in sterile physiological solutions, were prepared with a density (turbidity) of 0,5- 0,6, according to the standard of Mc Farland. Suspensions contained approximately 1,5- 2,0x10 CFU/ml, i.e. colony-forming units /ml.
  • Inoculums (within 15 minutes after preparation) were used for inoculation of the dense nutrient mediums in Petri dishes mentioned above.
  • the inoculum was placed by a pipette on the surface of a nutrient medium in a volume of 1-3 ml and was distributed on the surface at regular intervals. Slightly opened Petri dishes were dried at room temperature during 15-20 minutes.
  • the disks processed with tested polish compositions (Examples 1.4-1.7) were placed on the surface of a nutrient medium (with the help of a sterile tweezers) after inoculation. Uniform and close contact of disks to a surface of environment was provided.
  • Petri dishes were placed in the thermostat and were incubated at temperatures of 25-35°C over 18-72 hours (depending on the kind of tested microorganism) directly after application of disks.
  • Results of the tests testify the presence of zones of full suppression of visible growth of test-microorganisms (bacteria and funguses) around of disks with the tested polish compositions containing bactericidal agents.
  • Presence and size of the zones of growth inhibition of test-microorganisms testifies the stable and essential bactericidal and fungicidal effect in the tested polish compositions, intended for treatment of nails and nail platens for preventive avoidance and treatment of superficial mycosis.

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Abstract

L'invention concerne le domaine de la médecine, de l'hygiène, de la cosmétologie et concerne des produits qui peuvent être utilisés en tant qu'émail bactéricide préventif pour le traitement des ongles. Les vernis bactéricides préventifs pour le traitement des ongles de l'invention contiennent une substance filmogène hydrosoluble, un agent bactéricide et un solvant polaire. Des poudres de bentonite sont utilisées en tant qu'agent bactéricide. La bentonite est nanostructurée jusqu'à une taille de particules de pas plus de 150 nm et est intercalée par des ions de métaux Ag+ ou/et Cu2+ et Zn2+. La bentonite contenant les ions de métal est introduite dans une émulsion préparée de façon préliminaire de la substance filmogène, sous la forme d'un hydrosol à 4 à 10 %. Le vernis pour ongles de l'invention a le rapport suivant de composants, en % en poids : substance filmogène 1,0 à 10,0 ; agent bactéricide 0,2 à 1,5 ; solvant polaire : le reste. Grâce à la réalisation de l'invention, une protection efficace d'ongles traités contre un large spectre de bactéries et de champignons est obtenue, avec des vernis ayant un faible coût de fabrication. Le vernis peut être utilisé pour traiter des ongles et des corps d'ongles, pour la prévention et le traitement de mycoses superficielles et de processus inflammatoires.
PCT/EP2011/055666 2010-07-28 2011-04-12 Vernis bactéricide préventif pour le traitement des ongles WO2012013366A2 (fr)

Applications Claiming Priority (2)

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RU2010131406 2010-07-28
RU2010131406/15A RU2432158C1 (ru) 2010-07-28 2010-07-28 Профилактический бактерицидный лак для обработки ногтей

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WO2012013366A3 WO2012013366A3 (fr) 2012-03-22

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10172811B2 (en) 2013-02-07 2019-01-08 Polichem Sa Topical antifungal composition for treating onychomycosis
WO2024077670A1 (fr) * 2022-10-10 2024-04-18 浙江丰虹新材料股份有限公司 Agent bactéricide et antifongique efficace à base d'acide silicique nano-stratifié non toxique et son procédé de préparation et son utilisation

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0226984A1 (fr) 1985-12-19 1987-07-01 Hoechst Aktiengesellschaft Vernis à ongles antimycotique
US5120530A (en) 1989-02-24 1992-06-09 Hoffmann-La Roche Inc. Antimicotic nail varnish containing amorolfine in quaternary ammonium acrylic copolymer
US5487776A (en) 1994-03-17 1996-01-30 Nimni; Marcel Anti-fungal nail lacquer and method therefor
WO1999039680A1 (fr) 1998-02-09 1999-08-12 Macrochem Corporation Vernis a ongles antifongique et son procede d'utilisation
RU2245715C2 (ru) 2000-07-24 2005-02-10 Полишем С.А. Композиция лака для ногтей
RU2311186C1 (ru) 2006-02-06 2007-11-27 Леснова Наталья Витальевна Биологически активный компонент, обладающий противогрибковым действием, и противогрибковый лечебно-косметический лак демиктен на его основе
RU2330673C1 (ru) 2006-11-22 2008-08-10 Закрытое акционерное общество "Институт прикладной нанотехнологии" Способ получения антимикробного препарата

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FR2805745B1 (fr) * 2000-03-01 2002-12-13 Bergerac Nc Compositions pour vernis a ongles antifongiques et antiseptiques
DE10322444A1 (de) * 2002-05-23 2003-10-16 Schott Glas Formulierung kosmetischer Produkte mit Glaspulver
RU2358718C2 (ru) * 2003-03-21 2009-06-20 Нексмед Холдингс, Инк. Противогрибковое покрытие для ногтей и способ его применения
DK2343304T3 (en) * 2005-02-16 2015-06-29 Anacor Pharmaceuticals Inc BIOCIDE BORONOPHTHALIDE COMPOUNDS

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0226984A1 (fr) 1985-12-19 1987-07-01 Hoechst Aktiengesellschaft Vernis à ongles antimycotique
US5120530A (en) 1989-02-24 1992-06-09 Hoffmann-La Roche Inc. Antimicotic nail varnish containing amorolfine in quaternary ammonium acrylic copolymer
US5487776A (en) 1994-03-17 1996-01-30 Nimni; Marcel Anti-fungal nail lacquer and method therefor
WO1999039680A1 (fr) 1998-02-09 1999-08-12 Macrochem Corporation Vernis a ongles antifongique et son procede d'utilisation
RU2245715C2 (ru) 2000-07-24 2005-02-10 Полишем С.А. Композиция лака для ногтей
RU2311186C1 (ru) 2006-02-06 2007-11-27 Леснова Наталья Витальевна Биологически активный компонент, обладающий противогрибковым действием, и противогрибковый лечебно-косметический лак демиктен на его основе
RU2330673C1 (ru) 2006-11-22 2008-08-10 Закрытое акционерное общество "Институт прикладной нанотехнологии" Способ получения антимикробного препарата

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10172811B2 (en) 2013-02-07 2019-01-08 Polichem Sa Topical antifungal composition for treating onychomycosis
WO2024077670A1 (fr) * 2022-10-10 2024-04-18 浙江丰虹新材料股份有限公司 Agent bactéricide et antifongique efficace à base d'acide silicique nano-stratifié non toxique et son procédé de préparation et son utilisation

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RU2432158C1 (ru) 2011-10-27

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