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WO2011095427A1 - Procédé pour cultiver des micro-organismes - Google Patents

Procédé pour cultiver des micro-organismes Download PDF

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Publication number
WO2011095427A1
WO2011095427A1 PCT/EP2011/051126 EP2011051126W WO2011095427A1 WO 2011095427 A1 WO2011095427 A1 WO 2011095427A1 EP 2011051126 W EP2011051126 W EP 2011051126W WO 2011095427 A1 WO2011095427 A1 WO 2011095427A1
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WO
WIPO (PCT)
Prior art keywords
nutrient solution
microorganisms
separation plant
plant according
electrodes
Prior art date
Application number
PCT/EP2011/051126
Other languages
German (de)
English (en)
Inventor
Werner Hartmann
Original Assignee
Siemens Aktiengesellschaft
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Siemens Aktiengesellschaft filed Critical Siemens Aktiengesellschaft
Publication of WO2011095427A1 publication Critical patent/WO2011095427A1/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/12—Unicellular algae; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/02—Photobioreactors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/02—Separating microorganisms from the culture medium; Concentration of biomass
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/02—Separating microorganisms from their culture media
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves

Definitions

  • the invention relates to a method for growing microorganisms and to a separation system for separating microorganisms from a nutrient solution according to claim 7 and a plant for growing microorganisms according to claim 17. Using solar energy, a larger
  • Fer ⁇ ner also fuels, such as biodiesel can be generated from biomass.
  • the production of biomass using solar energy also consumes carbon dioxide to a greater extent.
  • This carbon dioxide can be recovered from insbeson ⁇ particular already incurred combustion processes, wherein the environmental pollution caused by CO 2, which is held such combustion processes, can be reduced.
  • accumulating carbon dioxide may already be bound by the producti on ⁇ biomass or are converted to innocuous products climate.
  • the biomass thus obtained can be used for other purposes.
  • both mechanical separators usually disc separators, are used to separate cultured algae, bacteria or yeast cultures from the nutrient medium.
  • the object of the invention is to provide a method and a separation plant, can be separated in the Mikroorganis ⁇ men from a liquid nutrient solution and thereby the energy consumption over the prior art is significantly reduced.
  • the solution of the problem consists in a method for growing microorganisms having the features of claim 1 and in a separation plant for the separation of microorganisms having the features of claim 7 and a plant for growing microorganisms according to claim 17.
  • the method for growing microorganisms according to Claim 1 comprises the following steps:
  • the first step involves the preparation of a nutrient solution and the inoculation of the nutrient solution with the cells of the microorganism to be grown.
  • a Ver ⁇ increase of microorganisms takes place. This is followed by a separation of the increased microorganisms from the nutrient solution, wherein the separation of the microorganisms they are exposed to a pulsed electric field.
  • the pulse duration of the electrical tric field is between 100 ns and 10 ys.
  • ⁇ sondere is the pulse duration between 500 ns and 5 ys.
  • This separation plant comprises a sedimentation tank and high voltage electrodes that dip into a nutrient solution and a high voltage generator.
  • this separation plant comprises a supporting beam, on which the high-voltage electrodes are arranged ⁇ and movably mounted to the settling tank.
  • electrically conductive grounded shielding elements are provided with respect to the high voltage electrodes. This ensures that forms a defined electric field strength in the vicinity of the high-voltage ⁇ electrodes, without the water volume of the thickener is exposed at other locations to dangerous for humans, animals or equipment high voltage potential.
  • the one or more shielding elements are preferably configured in the form of a wire, which is arranged in an electrically insulated manner around the high-voltage electrode .
  • the wire can be arranged at a distance of between 15 cm and 45 cm from the high-voltage electrode.
  • the wire can also be designed in the form of a wire mesh.
  • the shielding member may include a wire basket, the bezüg ⁇ Lich the high voltage electrode is stationary arranged. In this case, the high voltage electrodes move within the wire basket.
  • the shield at the high voltage electrode is arranged directly and be ⁇ moved together therewith through the nutrient solution.
  • Another component of the invention is a plant for the cultivation of microorganisms, which comprises an already described separation plant according to one of claims 7 to 16.
  • Figure 1 is a schematic representation of a system for
  • Figure 2 is a schematic representation of a vaccination with
  • Electrodes for generating a pulsed electric field before seeding are Electrodes for generating a pulsed electric field before seeding
  • FIG. 3 shows the schematic representation from FIG. 2 during the vaccination process
  • Figure 4 is a schematic representation of a Inoculation, wherein the electrodes in the form of Rohrelekt ⁇ roden are also arranged in a channel,
  • Figure 5 is a schematic representation of a vaccination after
  • FIG. 4 in which the electrode is designed in the form of a coaxial electrode
  • FIG. 6 shows a bioreactor in which electrodes are provided
  • FIG. 7 shows part of a bioreactor, namely a bubble reactor for mixing the nutrient solution and introducing carbon dioxide,
  • FIG. 8 shows a bioreactor for cultivating microorganisms in the form of a hose reactor
  • FIG. 9 shows a bioreactor for cultivating microorganisms in the form of a plate reactor
  • FIG. 10 shows a bioreactor for cultivating microorganisms in the form of a tubular reactor
  • FIG. 11 shows the arrangement of electrodes on a housing wall of a bioreactor in the form of conductor tracks
  • FIG. 12 shows the arrangement of electrodes on a housing wall of a bioreactor in the form of wires which are fastened to the surface of the housing wall or are embedded in the housing wall,
  • FIG. 13 shows the arrangement of electrodes on a housing wall in the form of conductor tracks, arranged inside and outside,
  • Figure 14 is a view in the direction of the arrow XIII of FIG
  • FIG. 15 shows the same view of a housing wall according to FIG. 13 along the arrow XIII, wherein the electrodes are arranged inside and outside but crossed over.
  • FIG. 16 shows a separation plant with sedimentation tanks and high-voltage electrodes
  • FIG. 17 is a cross-sectional view of the separation plants according to FIG. 1.
  • FIG. 18 shows a detailed representation of the process from FIG. 1 with a dehydrating plant for microorganisms.
  • a nutrient solution is first treated in a Impfan ⁇ would be 2, to be ⁇ write in more detail, different forms of electrical a Field is treated, wherein unwanted microorganisms are removed before vaccination from the vaccine or hindered in their reproduction.
  • the nutrient solution 3 is brought into a bioreactor 25 after vaccination with vaccine cells 4 (see FIG.
  • the bioreactor 25 is schematically shown as a dome shown here (bubble reactor 42) in which the ge ⁇ inoculated nutrient solution is prepared 3, ver ⁇ interspersed with carbon dioxide and is mixed.
  • the actual multiplication of the microorganisms takes place, for example, in a hose reactor 44, which is exposed in particular to solar radiation and in which the actual propagation of the microorganisms takes place.
  • the walls of the reactor 25 may be provided with electrodes 28 which generate a pulsed electric field.
  • the nutrient solution 3 with the microorganisms in a separation plant 50 given.
  • an electric field can act on the microorganisms, so that they sink to the bottom and settle there as a concentrated slurry.
  • the so deposited microorganisms usually algae
  • these algae are preferably exposed to a strong electric field, so that their cell wall is irreversibly destroyed. This process is called electroporation.
  • the so-damaged algae are placed in a Abpressbecken 78, wherein the cell water is forced out of the cells by high pressure. In this way, the algae lose a high proportion of their water and can be further processed as comparatively dry biomass for further purposes. They can be used in this pressed-off form as a supplier product for pharmaceutical see products or for chemical products. However, they can also be burned in a thermal plant. Although carbon dioxide is produced during thermal combustion of the algae mass, it has previously been added to the algae growth of the nutrient solution. A combustion process in this case would be almost carbon dioxide neutral.
  • FIGS. 2 and 3 schematically show a seedling 2, in FIG. 2 before a seeding process, in FIG. 3 during a seeding process.
  • the Impfanläge 2 comprises a Impf ⁇ basin 5, in the electrodes 6, here in the form of Parallelplat- tenelektroden 7, are arranged.
  • a nutrient solution 3 is contained in the Impfbecken 5.
  • the nutrient solution 3 contains not only desired nutrients but also other unwanted foreign biological cells, such as unwanted algae, bacteria or yeast cells.
  • a pulsed electric field 8 such cells are sustainably damaged in the nutrient solution 3, so that their growth during the ripening process of the desired microorganisms, in this case the algae, is prevented.
  • the applied electric field 8 is a pulsed electrical ⁇ sches field having a field strength that is greater than 1 kilo electron volts. Depending on the type of unwanted cells to be eliminated, however, the electric field can become significantly stronger and up to 100 kV / cm. At a relatively low field strength, a comparatively long pulse ⁇ as the pulsed electric field is selected.
  • the pulse duration is about 1 ms for a field of 1 kV / cm and can be shortened up to 10 ns if the electric field is greater than 10 kV / cm.
  • the electric field strength is dependent on a flow ⁇ rate of the nutrient solution 3 through the sedimentation tank 5, and by egg NEN channel 12, it is also dependent on the cross-section ⁇ area and the volume of the electrode system and, as already mentioned, on the type of to be eliminated unwanted cells.
  • the pulse repetition rates of the pulsed electrical of 8 are between two pulses / s up to fifty pulses / s. Suitable high-voltage amplitudes are in the range of 10 kV to over 100 kV, at currents of several 100 A up to 10 kA.
  • FIGS. 2 and 3 there is further shown a seed nozzle 9 which is shown schematically to illustrate the addition of seed cells 4 (see FIG.
  • the nutrient solution 3 can be pumped through a channel 12, in which electrodes 6 in the form of tubular electrodes 10 are arranged. These tube electrodes 10 are interrupted so that they are arranged in pairs and generate an electric field 8. As the nutrient solution flows through the tube electrodes 10 unwanted cells in the nutrient solution 3 are rendered harmless. After passing through the tubular electrode 10, the seed cells 4 of the desired microorganism can be so genzellen Al ⁇ inserted through the injection nozzle. 9 Subsequently, the nutrient solution 3 is pumped into the bioreactor 25, where the further processing and the multiplication of the algae takes place.
  • a vaccination process is likewise shown in FIG. 5, wherein coaxial electrodes 11 are used as an alternative to the tube electrodes 10 in FIG. 4, which run as concentric tubes, wherein the nutrient solution 3 passes through both tubes simultaneously and the electrical Field 8 is created between the tubes. Also after passing through the electrode system 11, the vaccination is carried out by the Impfdüse 9 and a forwarding of the nutrient solution 3 in the bioreactor 25th
  • FIG. 6 Another alternative is described in FIG. 6, wherein the nutrient solution 3 can be inoculated in the bioreactor 25, wherein the bioreactor 25 is designed in the form of a bubble reactor 42, which is part of the bioreactor 25.
  • this nutrient solution is mixed, processed and shipping ⁇ hen with CO 2.
  • this bubble reactor 24 are here exemplified Elek- electrodes 6 arranged in the form of parallel plate electrodes 7, which frees the nutrient solution by the electric field 8 of unwanted cells before the vaccination process with the Impfdüse 9.
  • FIG. 7 shows a part of a bioreactor 25 is shown, namely, a bubble reactor 42.
  • the bubble possibly already with the cell of a Musses microorganisms, in particular algae cells inoculated nutrient solution is 3 ge ⁇ mixed and processed.
  • a circulating system 38 is seen before ⁇ and an inlet 46 for carbon dioxide (C0 2 ) and an outlet 47 for the elimination of nitrogen (N 2 ).
  • the Bla ⁇ senreaktor 42 has a housing wall 27, in the optional, if required, electrodes can be provided 28th These electrodes 28 are used to generate an electric field, can be killed by the unwanted microorganisms such as yeast cells, bacteria or unwanted algae, or can be inhibited in their propagation such that they do not cover a surface 31 of the Reak ⁇ torwand in the form that the incoming sunlight could be permanently obscured. The so-called biofouling is prevented by this measure.
  • FIG. 8 shows by way of example a further component of the bioreactor 25, this being a hose reactor 44, which is designed in the form of flexible plastic films.
  • the nutrient solution 3 with the microorganisms 37 multiplying therein, ie in the form of the algae 37 to be cultivated in this embodiment, is pumped by the bubble reactor 42 into the tube reactor 44, where the nutrient solution 3 with the algae 37 remains until the algae 37 have multiplied so much that they shield the nutrient solution 3 in which they are located from the sunlight in such a way that further growth is inhibited. This situation is usually present with an algae concentration of 5%.
  • the part of the bioreactor 25 described in FIG. 8, namely the hose reactor 44, is merely an exemplary embodiment of a bioreactor 25 in which the algae 37 can grow.
  • Alternative embodiments are again shown purely by way of example and schematically in FIGS. 9 and 10.
  • 9 is a Plattenre- illustrated actuator 43, wherein the nutrient solution 3 is disposed with the Mikroor ⁇ organisms 37 between two parallel, transparent plates such as glass plates or acrylic plates. Through the plates of the plate reactor 43 Suns ⁇ light can be incident, which is used for multiplication of algae under 37 exemplary of photosynthesis.
  • FIG. 10 is in the form of a tubular reactor 45, wherein transparent tubes serve to store the nutrient solution 3 with the multiplying algae 37.
  • the applied parameters of the pulsed electric field are of course dependent on the geometry of the bioreactor 25 and the process parameters selected therein. For example, it is important which Wandab ⁇ stand the walls 27 of the bioreactor 25 have, or whether the nutrient solution 3 circulates with the algae 37 in the bioreactor or whether it is stationary and how high the sun's rays is at the location of the bioreactor. It has been found to be expedient ⁇ SSIG that the pulsed electric field having a field strength of 0.5 kV / cm and 5 kV / cm.
  • the pulse duration in ⁇ is between 10 ns and 50 ys, wherein likewise dependent on the geometry of the bioreactor 25 and the geometry or the thickness of the housing wall 27 and to zer ⁇ interfering undesirable microorganisms, the total energy by the electrical field is introduced, should be varied. Therefore, it is also expedient to choose a low pulse duration with a high field strength and vice versa.
  • the applied pulse duration is between 5 pulses / s and 50 pulses / s. It should also be to ⁇ into account whether the nutrient solution 3 circulates in the reactor or whether it remains stationary.
  • 11 shows a section of a housing wall 27 is provided, where ⁇ are madestal- tet with the electrodes 28 in the form of conductor tracks 30th
  • the conductor tracks 30 can be vapor-deposited on the surface 31 of the housing wall 27, for example, by a coating method.
  • the conductor tracks 30 are arranged parallel to one another. It is expedient that the surface 31 of the housing wall 27 concealed by the electrodes 28 is kept as small as possible.
  • the electrodes shown in Figure 11 are thus shown relatively thick and therefore purely exem ⁇ plarisch, not to scale, to see.
  • the thickness of the electrodes 28 th ⁇ less than 5 mm be preferably less than 1 mm, the distance between the electrodes should be 28 to each other is less than 30 mm.
  • FIG. 12 shows a further exemplary illustration of a housing wall 27 of a bioreactor 25.
  • This may be, for example, a section of a tubular reactor 45.
  • the electrodes 28 are shown here in the form of wires 32, which may be attached on the one hand to the inside 34 of the Ge ⁇ housing wall 27.
  • the wires 32 at the nenwand 34 of the housing wall 27 are clamped in the vicinity of the surface on the surface.
  • wires 32 are placed on an outer side 33 of the housing wall 27 in the material of the housing wall 27. You can, for example, if it is han ⁇ punched in the housing wall 27 by a mold material are cast into the material.
  • FIG. 13 again shows a similar housing wall 27 as shown in FIG.
  • the electrodes 28 are again configured in the form of printed conductors 30 here.
  • an electrode 28 on the outer side 33 of the housing wall 27 is arranged on ⁇ and another, corresponding thereto electrode 28 on the inner side 34 of the housing wall 27 is arranged.
  • FIG 14 is an illustration of a detail of egg ⁇ ner housing wall 27 along the arrow XIII in Figure 13 gege ⁇ ben.
  • the electrodes 28, which are shown here in the form of conductor tracks 30, are arranged on the inner side 34
  • FIG. 15 the same view of the arrow XIII is shown in FIG. 13 as in FIG. 14. Only the electrodes 28, or again in the form of printed conductors 30, are likewise shown on the inner side 34 or the outer side 33 (again dashed) ).
  • the tracks 30 of Figure 15 are crossed at an angle to each other, resulting in many intersections of the electrodes to each other. A special alignment of the electrodes relative to one another is therefore unnecessary, which reduces the manufacturing requirements for the electrodes and thus the manufacturing costs of the housing walls.
  • FIG. 16 shows a separation plant 50 which comprises a sedimentation tank 52 into which the nutrient solution 3 saturated with algae is pumped in the bioreactor 25 after algae cultivation.
  • a separation plant 50 which comprises a sedimentation tank 52 into which the nutrient solution 3 saturated with algae is pumped in the bioreactor 25 after algae cultivation.
  • the algae or with other micro Orga ⁇ mechanisms is very small, airborne particles, which do not settle readily. Therefore, it is difficult to mechanically separate the algae from the nutrient solution 3.
  • the application of short pulsed high electric fields in the nutrient solution with the algae temporarily stops their metabolism.
  • the thus anesthetized algae or microorganisms sink to the bottom of the settling pond 52 (in the separation plant 50 treated algae are provided from the figure 16 with the reference numeral 51).
  • a high voltage electrode 53 is introduced into the nutrient solution 3 and high voltage pulses are applied to the electrodes 53 via a semiconductor switching technique. Since the cell walls of the algae 51 do not necessarily have to be permanently destroyed in these method steps, a rela ⁇ tively weak electric field, which is based on the size of the settling tank 52 or its height and based on the
  • Concentration of algae 51 and depending on the species of algae is usually between 100 V / cm and 10 kV / cm.
  • the case facing to ⁇ pulse duration is between 100 ns and about 10 ys.
  • a rather shorter pulse duration is used in said interval, and a longer pulse duration is used for lower electric fields.
  • relatively inexpensive semiconductor switching techniques as high voltage generators. This makes this process particularly economical.
  • a relatively small amount of energy is needed (compared to the prior art), on the other hand is at the side facing to ⁇ technique to provide the high voltage electrode and the high voltage generators to relatively inexpensive investment.
  • FIG. 16 shows a three-dimensional representation of a round settling basin 52, which comprises a rotatably mounted supporting beam 54, on which in turn the high-voltage electrodes 53 are arranged.
  • the support beam 54 rotates through the round sedimentation tank 52, pulling the high voltage electrodes through the nutrient solution so that the algae 51 floating therein are gradually exposed to the electric field.
  • the algae 51 are anesthetized and cease their metabolism and sink as Algenbrei 51 on the bottom of the settling tank 52 (see Figure 3).
  • the height of the water level in settling tank 52 is preferably about 50 cm to 1 m.
  • the high voltage electrodes 53 have shielding elements 55. These shielding elements 55 are configured in the form of wires 56.
  • the electrically conductive shielding elements 55 are electrically insulated from the high-voltage electrodes and grounded. They cause, that forms a defined electric field strength in the vicinity of the high voltage electrodes 53, without the water volume of the Eindi ⁇ thickener (settling tank 52) is exposed tential hazardous to humans, animals or plant parts Hochtentspo- elsewhere.
  • the shielding elements 55 are left and right shown schematically.
  • the shielding elements 55 are configured in the form of wires 56, which are arranged at a distance of approximately 15 cm to 45 cm around the high-voltage electrode 53. These wires 56, which are madestal ⁇ tet as wire mesh, move with the high voltage electrode 53 and the support bar 54 continuously through the nutrient solution. 3
  • FIG. 17 On the right side of Figure 17, an alternative training is illustrated design, wherein the screening elements 55 'configured in the form of a wire basket 57 that surrounds the radius described by the Hal ⁇ tebalken 54 and the high-voltage electrodes 53 within the settling tank 52nd
  • This wire basket 57 has the same effect as the shielding elements 56, it is also grounded and insulated with respect to the high voltage ⁇ electrodes 53.
  • a typical mesh size of the wire ⁇ basket 57 is 0.5 cm.
  • In the shield itself is preferred to resort to a nutrient solution over the inert mate ⁇ rial, such as stainless steel.
  • a combination of wires 56 and one or more wire baskets 57 as shielding elements 55 in a separation basin are likewise expedient.
  • the pulse repetition rate is a function of the geometry of the settling tank, the rotation speed or Be ⁇ motion speed of the stop bar 54 and the Konzentra ⁇ tion of algae 51 in the nutrient solution 3 chosen so that entrapped in the volume of algal cells in each case at least one ⁇ times, Preferably, three to five times, possibly even up to 20 times, are subjected to the high voltage pulse before they sink to the bottom of the settling tank 52.
  • the settled algal cells 51 (see FIG Pumping channel 76 is sucked out of the settling tank 52.
  • the electrodes 77 are tube electrodes which are interrupted and connected in series, the electrodes 77 'are intended to represent parallel plate electrodes. Coaxial tube electrodes would also be expedient.
  • the pumping channel 76 is designed in a preferred manner with an almost rectangular cross section may have a width of 10 cm to about 1 m aufwei ⁇ sen.
  • the pumping channel 76 is designed rather rectangular with egg ⁇ ner higher width and a shallower height.
  • the electric field is applied by the electrodes 77.
  • the electric field is chosen so that it has a field strength of 1 kV / cm up to 10 kV / cm depending on the biological nature of the microorganisms to be treated.
  • Typical current amplitudes are 10 kA up to 100 kA
  • the pulse durations are between 10 ns and 50 ys.
  • the combination of the mentioned field strengths and the current amplitudes and the pulse duration leads to effective applied amounts of energy that are so low that high-power semiconductor switches, such.
  • thyristors can be used as high voltage generators. This leads to a significant reduction in the investment costs for high-voltage generators, which reduces the total operating costs of the plant for algae cultivation. If higher current amplitudes are required, for example, for larger channel widths, this can be achieved by the parallel connection of several semiconductor switches.
  • This treatment with electric fields results in electroporation of the aigen cell walls, resulting in micropores in the cell walls.
  • This so treated concentrated algae mass 79 is pumped into a Abpressbecken 78, in which the actual pressing out of the cell water or dehydration is carried out by a press die 80 shown schematically. Due to the already existing micropores in Algae cells can be removed with a significantly lower pressure than would be the case without the use of electroporation and without the application of electric fields.
  • a highly dewatered biomass ⁇ can be thermally utilized directly in principle be already or can be supplied as a raw material in the chemical industry or the pharmaceutical industry in this form, depending on the type of microorganism ses.
  • a further thermal drying step may be necessary, but the energy expenditure is significantly lower than a conventional drying process in a conventional Algenzüchtungs ⁇ method according to the prior art.
  • the thus dried algae mass can be transported with less effort, for example as a pumpable medium in tanker trucks. Thus, this can be transported to the energy sources for further drying - if necessary - or be transported in power plants for further thermal utilization.

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Abstract

La présente invention concerne un procédé pour cultiver des micro-organismes. Le procédé comprend les étapes suivantes. Une solution nutritive (3) est tout d'abord produite, puis cette solution nutritive (3) est inoculée avec des cellules du micro-organisme à cultiver. Au cours d'une étape ultérieure ont lieu la multiplication du micro-organisme puis la séparation des micro-organismes issus de la multiplication pour les isoler de la solution nutritive (3). Lors de la séparation des micro-organismes de la solution nutritive (3), on leur applique un champ électrique pulsé.
PCT/EP2011/051126 2010-02-08 2011-01-27 Procédé pour cultiver des micro-organismes WO2011095427A1 (fr)

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DE201010007167 DE102010007167A1 (de) 2010-02-08 2010-02-08 Verfahren zum Züchten von Mikroorganismen
DE102010007167.6 2010-02-08

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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999011771A1 (fr) * 1997-09-04 1999-03-11 Science Research Laboratory, Inc. Separation de cellules par utilisation de champs electriques
WO2008098298A1 (fr) * 2007-02-16 2008-08-21 Iogenyx Pty Ltd Procédés d'amélioration de culture d'organismes aquatiques
WO2010104922A1 (fr) * 2009-03-10 2010-09-16 Srs Energy Fractionnement d'une biomasse d'algues
WO2010123903A1 (fr) * 2009-04-20 2010-10-28 Originoil, Inc. Systèmes, appareil et procédés pour obtenir des produits intracellulaires et une masse cellulaire et des débris à partir d'algues et produits dérivés, et leur procédé de mise en oeuvre

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999011771A1 (fr) * 1997-09-04 1999-03-11 Science Research Laboratory, Inc. Separation de cellules par utilisation de champs electriques
WO2008098298A1 (fr) * 2007-02-16 2008-08-21 Iogenyx Pty Ltd Procédés d'amélioration de culture d'organismes aquatiques
WO2010104922A1 (fr) * 2009-03-10 2010-09-16 Srs Energy Fractionnement d'une biomasse d'algues
WO2010123903A1 (fr) * 2009-04-20 2010-10-28 Originoil, Inc. Systèmes, appareil et procédés pour obtenir des produits intracellulaires et une masse cellulaire et des débris à partir d'algues et produits dérivés, et leur procédé de mise en oeuvre

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