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WO2011071353A2 - Tube pour centrifugeuse - Google Patents

Tube pour centrifugeuse Download PDF

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Publication number
WO2011071353A2
WO2011071353A2 PCT/KR2010/008968 KR2010008968W WO2011071353A2 WO 2011071353 A2 WO2011071353 A2 WO 2011071353A2 KR 2010008968 W KR2010008968 W KR 2010008968W WO 2011071353 A2 WO2011071353 A2 WO 2011071353A2
Authority
WO
WIPO (PCT)
Prior art keywords
chamber
centrifuge tube
intermediate chamber
upper chamber
diameter
Prior art date
Application number
PCT/KR2010/008968
Other languages
English (en)
Korean (ko)
Other versions
WO2011071353A3 (fr
Inventor
전민용
Original Assignee
Jeon Min-Yong
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Jeon Min-Yong filed Critical Jeon Min-Yong
Priority to EP10836248.4A priority Critical patent/EP2511011A4/fr
Priority to US13/513,906 priority patent/US20120251411A1/en
Priority to KR1020127012529A priority patent/KR101289535B1/ko
Priority to JP2012543030A priority patent/JP2013514874A/ja
Priority to CN2010800554604A priority patent/CN102844121A/zh
Publication of WO2011071353A2 publication Critical patent/WO2011071353A2/fr
Publication of WO2011071353A3 publication Critical patent/WO2011071353A3/fr

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B1/00—Centrifuges with rotary bowls provided with solid jackets for separating predominantly liquid mixtures with or without solid particles
    • B04B1/10—Centrifuges with rotary bowls provided with solid jackets for separating predominantly liquid mixtures with or without solid particles with discharging outlets in the plane of the maximum diameter of the bowl
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5021—Test tubes specially adapted for centrifugation purposes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5082—Test tubes per se
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/10—Apparatus for enzymology or microbiology rotatably mounted
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0822—Slides
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0832—Geometry, shape and general structure cylindrical, tube shaped
    • B01L2300/0838—Capillaries
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0848—Specific forms of parts of containers
    • B01L2300/0858—Side walls

Definitions

  • the present invention relates to a centrifuge tube, and more particularly to a centrifuge tube that facilitates the separation of certain components.
  • Figure 1 is an exemplary view of a conventional centrifuge tube, in a solution in which various substances are mixed, especially when human or animal blood is collected and centrifuged, red blood cells, white blood cells, platelets, and other plasma components in the blood are unique. Due to one specific gravity difference, the layered layers are placed in order from the bottom of the centrifuge tube 10 to the heavy ones.
  • white blood cells and platelets occupy about 1% of the total blood, including peripheral blood stem cells, including monocytes (monocytes).
  • peripheral blood stem cells including monocytes (monocytes).
  • monocytes monocytes
  • the rest are located in the upper layer, called serum and fibrinogen.
  • the centrifuge tube is removed and the stopper is opened again to separate the desired material from the separated material by the specific gravity difference of each component through a pipette, capillary or needle, and the remaining components are stored separately or discarded or recycled.
  • the buffy coat layer containing the stem cells to be separated is only about 1% of the total solution, and when separated in a centrifuge tube with a substantially wide diameter, the buffy coat layer is thinly laid on the red blood cells as a sheet of paper. It is a very difficult task.
  • FIG 2 is an exemplary diagram of a blood collection bag, commonly referred to as a blood bag, by centrifugation while temporarily storing the blood collected through blood collection to separate the blood into red blood cells leukocyte platelets and plasma components for each component.
  • a blood bag by centrifugation while temporarily storing the blood collected through blood collection to separate the blood into red blood cells leukocyte platelets and plasma components for each component.
  • these components which are about 1% of the whole blood, form a thin white buffy coat layer upon first centrifugation. Separation poses a very difficult problem in practice.
  • FIG 3 is a cross-sectional view of another conventional centrifuge tube, in which the diameter of the intermediate chamber 40 in the diameter of the centrifuge tube 10 is significantly smaller than that of the other two chambers 30 and 50. .
  • This thinly formed intermediate chamber 40 is very easy to separate when the buffy coat layer is formed here compared with the other two chambers 30, 50 formed.
  • a separate plug 60 is formed as a means for moving the buffy coat layer to the intermediate chamber 30.
  • a separate block 70 is formed surrounding the centrifuge tube 10.
  • US Pat. No. 4,861,477 forms a plug in the lower part of the centrifuge tube to compensate for this disadvantage, so that the volume of the space where the red blood cells are separated can be adjusted.
  • another block component is formed outside the centrifuge tube, which increases the manufacturing cost of the centrifuge tube.
  • US Pat. No. 5,422,018 serves to protect the middle chamber portion of the narrow inner centrifuge tube by filling the medium between the concave inner centrifuge tube and the surrounding outer centrifuge tube.
  • the method is also cumbersome to operate and increases the manufacturing cost of the centrifuge tube.
  • Patent Publication No. 10-2006-0059849 discloses a method for preparing platelet rich plasma, which includes adding a water-soluble polymer to peripheral blood, and similar methods for separating monocytes from peripheral blood in a laboratory.
  • Ficoll-Hypaque a polymer of Ficoll (a sucrose polymer) and Hypaque (a polymer of sodium ditrizote)
  • the Ficoll-Hypaque has a specific gravity of 1.0778g / ml, and since monocytes are lighter than this, red blood cells are heavier than this, so they are separated using specific gravity.
  • the present invention is to provide a centrifuge tube that can cause efficient component separation due to the specific gravity difference of each substance in the solution containing biological chemical components including peripheral blood.
  • the present invention is a centrifuge tube having an outer wall for accommodating a flow material in the inner space and separating the components according to the specific gravity of the flow material contained therein, the upper chamber having an inlet at the top and having a constant internal volume; Intermediate by centrifugal force during centrifugation in a centrifuge tube having an intermediate chamber communicating with a lower portion of the upper chamber and having a diameter smaller than the upper chamber, and a lower chamber communicating with a lower portion of the intermediate chamber and having a diameter larger than the intermediate chamber.
  • One or more support pieces are integrally formed on the outer wall of the intermediate chamber to prevent deformation of the chamber.
  • the present invention is a centrifuge tube having an outer wall for accommodating a flow material in the inner space and separating the components according to the specific gravity of the flow material contained therein, the upper chamber having an inlet at the top and having a constant internal volume;
  • the centrifuge which is connected to the lower part of the upper chamber and has a diameter smaller than the upper chamber, and the 15ml and 50ml conical tubes are inserted, the lower chamber and the buoycoat layer are formed in the intermediate layer by one centrifugation.
  • the volume of the intermediate chamber and the upper chamber is formed at a constant ratio, and in order to prevent deformation of the intermediate chamber during centrifugal separation, one or more support pieces are integrally attached to the outer wall. This support piece has a lower end extending to the centrifuge bucket.
  • a feature of the present invention is that the upper chamber has a stopper to close the open inlet, which is made of a flexible material, in particular one of rubber, silicone, PVC, polyethylene, polypropylene or polymer.
  • a feature of the present invention is that a stopper blocking the top of the upper chamber allows the central portion to be convex outward to facilitate disinfection before inserting the syringe needle.
  • the stopper forms a binding jaw at the upper end of the upper chamber, and adds a cap having a binding groove coupled with the binding jaw so that the plug is not easily separated from the upper chamber.
  • a feature of the present invention is that the lower chamber is flexible because its bottom surface is thinner than the thickness of the side, which makes it possible to reduce the volume of the lower chamber if necessary in order to place the minute components into the intermediate chamber.
  • the present invention is a centrifugal pack for separating the components according to the specific gravity of the fluid contained in the interior, the outer material containing the flow material, the upper chamber having an inlet at the top and a constant internal volume, An intermediate chamber communicating with the lower part of the upper chamber and having a diameter smaller than the upper chamber, a lower chamber communicating with the lower part of the intermediate chamber and having a diameter larger than the intermediate chamber, and an upper chamber, an intermediate chamber, and an outer chamber forming the lower chamber A hole is formed and the hook hole is caught in the flat bucket with the loop.
  • the lower inlet formed in the lower part of the centrifugal pack is a centrifugal pack for separating the components according to the specific gravity of the fluid contained in the interior, the outer material containing the flow material, the upper chamber having an inlet at the top and a constant internal volume, An intermediate chamber communicating with the lower part of the upper chamber and having a diameter smaller than the upper chamber, a lower chamber communicating with the lower part of the intermediate chamber and having a diameter larger than the intermediate chamber
  • the present invention has an outer wall for receiving a fluid in the inner space, and covers the outer wall of the intermediate chamber of the centrifuge tube to separate the components according to the specific gravity of the fluid contained therein than the outer wall of the upper chamber and the lower chamber. It is to peel.
  • a feature of the present invention is a fibrin activating material such as thrombin, calcium divalent ion, glass beads or an anticoagulant such as heparin, ACDA, CPDA, EDTA, or a water-soluble high molecular compound or Ficoll-Hypaque that precipitates a specific material.
  • a fibrin activating material such as thrombin, calcium divalent ion or glass bead, or a fluorescent target material separation reagent or magnetic microbead using an antigen antibody reaction in a predetermined amount in a centrifuge tube. It is injected.
  • a feature of the present invention is that a display portion indicating the amount of the object to be centrifuged is formed on the outer surface of the upper chamber.
  • the present invention is a centrifuge tube having an upper chamber, an intermediate chamber, and a lower chamber, wherein the volume of the lower chamber is formed to be about 36% of the total volume of the centrifuge tube, and the volume of the intermediate chamber is formed to be 10 ⁇ 2%, The components to be extracted are collected in the intermediate chamber.
  • the present invention has the following effects.
  • the support piece When centrifugation is performed due to the specific gravity difference of the biological chemical substance to be separated, the support piece is formed on the side of the centrifuge tube so that the narrow diameter centrifuge tube withstands the high centrifugal force and the acceleration of gravity generated during the centrifugation.
  • centrifugation is possible due to the fine specific gravity difference which can only be achieved with a narrow diameter centrifuge tube.
  • a buffy coat layer containing a target stem cell is formed in an intermediate chamber having a narrow diameter so that the buffy coat layer can be efficiently separated. It became.
  • the simplely formed support piece eliminates the inconvenience of forming a separate external block or installing an intermediate medium as compared to the conventional method, and thus, it is possible to manufacture a centrifuge tube at a low price, and to remove it after using an artificial polymer. Fine centrifugation is possible without the hassle of the process, which makes the work in the laboratory easier.
  • the thickness of the bottom surface of the lower chamber of the centrifuge tube is made slightly thin so that the solution formed in the lower chamber can be pushed up to the intermediate chamber without the need for a separate screw or plug. It can be located in a narrow intermediate chamber.
  • a blood withdrawal cap can be formed for easy separation.
  • the centrifugation operation can be performed safely from biological contamination that may be introduced when the stopper is opened. There is an advantage.
  • the indicators indicating the amount of blood injected in the case of males or females, or indicators, symbols, etc., are displayed so that the operator can simply inject the desired stem cells into the indicators. It helps the buffy coat layer to separate into the intermediate chamber.
  • centrifuge which is the most widely used 15ml conical tube, which is currently used in various medical institutions and laboratories, it makes it easy to extract the desired component of buffy coat from blood by one centrifugation.
  • the buffy coat layer to be extracted when the blood is centrifuged using the present invention centrifuge tube that is, the volume of the lower chamber is formed to about 36% of the total volume of the centrifuge tube and the volume of the intermediate chamber is 10 ⁇ 2%. As it is collected in this intermediate chamber, it is very easy to extract.
  • Figure 2 is an illustration of a conventional blood collection bag
  • Figure 3 is a cross-sectional view of an example of a conventional centrifuge tube
  • Figure 4 is a perspective view of one embodiment of a centrifuge tube of the present invention
  • Figure 5 is a perspective view of one embodiment of a centrifuge tube of the present invention
  • FIG. 6 is a cross-sectional view taken along the line A-A of FIG.
  • FIG. 7 is a cross-sectional view taken along the line C-C of FIG.
  • FIG. 8 is a cross-sectional view taken along the line B-B of FIG.
  • Figure 10 is a cross-sectional view of one embodiment of a centrifuge tube of the present invention.
  • Figure 11 is a cross-sectional view of one embodiment of a centrifuge tube of the present invention
  • FIG. 12 is a view for explaining an embodiment of the blood collection bag of the present invention.
  • Figure 13 is a view for explaining one embodiment of the blood collection bag of the present invention
  • FIG. 14 is a view for explaining an embodiment of the blood collection bag of the present invention.
  • 15 is a view for explaining an embodiment of the blood collection bag of the present invention.
  • Figure 16 is a perspective view of a flat bucket of the present invention
  • FIG 17 is a perspective view of the centrifuge tube of the present invention
  • Figure 18 is a perspective view of one embodiment of a centrifuge tube of the present invention.
  • 19 is a cross-sectional view taken along the line A-A of FIG.
  • 21 is a cross-sectional view taken along the line B-B of FIG.
  • FIG. 22 is a cross-sectional view taken along the line D-D in FIG. 17
  • Figure 23 is a perspective view of one embodiment of a centrifuge tube of the present invention.
  • Figure 24 is an exploded perspective view of one embodiment of a centrifuge tube of the present invention
  • Figure 25 is a front view of one embodiment of a centrifuge tube of the present invention.
  • FIG. 26 is a cross-sectional view taken along the line AA ′ of FIG. 25;
  • Figure 4 is an embodiment of the centrifuge tube of the present invention
  • the lower chamber 50 is a centrifugal separation of the material with the highest specific gravity
  • the intermediate chamber 40 is a specific gravity of the material is collected in the middle
  • the specific gravity is the first It has an upper chamber where light material gathers
  • a support piece 80 which supports it next to the intermediate chamber and is connected to the upper chamber and the lower chamber.
  • the support piece 80 may be marked with a scale to confirm the volume capacity of the solution.
  • At least one support piece 80 is formed, and preferably three pieces are formed, and the support piece 80 is formed in the upper chamber 30 or the lower chamber as well as the intermediate chamber 40 side of the centrifuge tube. 50 may also be formed on the side.
  • the support piece 80 may be marked with a scale to confirm the volume capacity of the solution.
  • the top of the centrifuge tube is formed with a stopper 90 through which the needle can penetrate, such as rubber, silicone, polyethylene, polypropylene or PVC, and the stopper 90 is a fitting or centrifuge tube 10. It can be selected from the method of forming a screw thread in the form of a screw thread to be symmetrical to it.
  • centrifuge tube of the present invention has the following values.
  • the diameter and length of the upper chamber 30 of the centrifuge tube 30 and the lower chamber 50 of the intermediate chamber 40 at a constant ratio, even if the conventional 15ml and 50ml centrifuge tubes are applied to all centrifuges are inserted, It is possible to easily form a buffy coat of the desired component in the blood in the intermediate chamber 40, without the need of a device, and the like.
  • the outer diameter of the centrifuge tube maintains the upper chamber 30 of 16mm ⁇ 2mm in the case of the centrifuge tube applied to the centrifuge adapter for 15ml
  • the outer diameter of the lower chamber 50 also maintains the 16mm ⁇ 2mm
  • intermediate chamber The outer diameter of the 40 is maintained at 6mm ⁇ 2mm
  • the volume of the lower chamber 50 is formed not more than 36% of the volume of the entire centrifuge tube, but the volume of the intermediate chamber 40 is 10 of the volume of the whole centrifuge tube Form% ⁇ 2%.
  • the outer diameter of the upper chamber 30 is maintained at 28 mm ⁇ 2 mm
  • the outer diameter of the lower chamber 50 is also maintained at 28 mm ⁇ 5 mm
  • the outer diameter of the intermediate chamber 40 is Is maintained at 6mm ⁇ 2mm
  • the volume of the lower chamber 50 is formed not more than 36% of the volume of the entire centrifuge tube, but the volume of the intermediate chamber 40 is 10% ⁇ 2% of the volume of the entire centrifuge tube To form. This always forms a buffy coat in the intermediate chamber 40 at the time of centrifuging blood regardless of the formation of the support piece 80 in the centrifuge tube.
  • the heavy red blood cell layer is collected in the lower chamber 50, and the buffy coat layer such as white blood cells and platelets is separated into the intermediate chamber 40.
  • the 80 prevents the intermediate chamber 40 from being deformed even at the high gravity acceleration g generated during centrifugation.
  • biological liquids such as blood
  • the stopper 90 By allowing biological liquids such as blood to be injected into the centrifuge tube without opening the stopper 90, the incorporation of viruses and bacteria can be prevented. This can be a closed operation in medical devices or biological devices that require strict sterilization to prevent contamination of a sample, culture, or centrifuge.
  • Figure 5 is another embodiment of the centrifuge tube of the present invention, to remove the lower chamber of Figure 4, the intermediate chamber 40 is extended to the bottom of the centrifuge tube so that it can be selected and used according to the characteristics of the biological solution It is. In other words, a small amount of solution with similar specific gravity is suitable for centrifugation. This can be used when more detailed centrifugation is required. For example, when the ratio of erythrocytes and platelet leukocytes is similar in the primary separated blood, it can be separated more finely and easily.
  • This type of centrifuge tube has an intermediate chamber in spite of the high gravity acceleration (g) pressure applied to the intermediate chamber 40 during centrifugation due to the support piece 80 formed on the side of the intermediate chamber 40 extending to the bottom. (40) prevents twisting or shaking, so that the contained blood is arranged neatly according to the specific gravity difference of each component, thereby helping to allow finer centrifugation.
  • the support piece 80 allows the capacity of the solution to be marked with a margin even if it is difficult to display the volume of the solution.
  • the end of the support piece 80 can form a wing portion extending outward to be in close contact with the bucket of the centrifuge, can be taken out of the centrifuge to stand on a flat floor to facilitate the separation operation.
  • the cross-sectional shape of the upper chamber 30 and the lower chamber 50 has a pentagonal shape, but it is not necessarily required. In some cases, the cross-sectional shape may be an inverted triangle, a triangle, or a shape close to a rectangle.
  • the thickness of the bottom surface of the lower chamber 50 is formed to be slightly flexible than the thickness of the wall surface, so that if the buffy coat layer is hung on the lower chamber 50, the bottom surface of the lower chamber 50 is flexible. By pushing the portion, the buffy coat layer can be moved toward the intermediate chamber 40 to facilitate separation. 5 and 7, the lower part of the support piece 80 formed on the side of the intermediate chamber 40 has a long end thereof and is formed to be in close contact with the bucket of the centrifuge. It serves to prevent shaking of the centrifuge tube.
  • At least one support piece 80 is formed on the surface of the intermediate chamber 40 having a small diameter.
  • FIG. 10 shows an intermediate chamber 40 with a narrow diameter upward for easy separation when the components to be separated in more detail are distributed in the middle or more
  • FIG. 13 shows that the components to be separated in a small amount of the solution as a whole. It can be used conveniently when finding a substance.
  • the support piece 80 is not only deformed in the centrifuge tube but also extends the wing portion of the support piece 80 in close contact with the centrifugal bucket so that the boundary layer of each of the centrifuged components is disturbed due to shaking. Will be prevented.
  • FIGS. 12, 13, and 14 are diagrams showing one example of the blood collection bag of the present invention, and a material of the blood collection bag 20 used for collecting and storing blood is made of PVC.
  • the blood collection bag 20 is divided into an upper chamber 31, an intermediate chamber 41, and a lower chamber 51, respectively.
  • the support piece 81 formed in the middle portion of the blood collection bag 20 prevents tearing or deformation of the intermediate chamber 41 having a small diameter and elongated shape due to the high gravity acceleration (g) generated during centrifugation. It also serves to fix the shape of the intermediate chamber 41 during the separation and sampling operation of each component to prevent the buffy coat layer rocking or drift.
  • FIG 16 is a perspective view of a flat plate bucket 120 that is caught on the rotor of the centrifuge, the blood collection bag 20 is caught by the ring 140 formed in the flat plate bucket 120. At this time, the hook hole 100 formed in the blood collection bag 20 corresponds to the ring 110 of the flat bucket 120.
  • the buffy coat layer in which platelets or leukocyte layers are collected in the centrifuged blood is gently pressed down the lower chamber 51 when the lower chamber 51 is formed, the buffy coat layer is slightly pushed upwards and is slightly Since it is formed on the chamber 41 side, it can be easily separated by using a needle or a capillary tube, and furthermore, since the plasma and erythrocyte platelet layers are separated in the intermediate chamber 41, which is narrow in diameter, the diameter is elongated.
  • the centrifuged blood components can be stored even if they are not transferred to separate containers or the like, and as shown in FIG. Through the inlet 92, red blood cells may be pulled out separately.
  • a water-soluble polymer compound that precipitates a specific substance or a separation substance such as Ficoll-Hypaque is added, or an anticoagulant such as heparin, ACDA, CPDA, EDTA, or thrombin, calcium divalent ion, Fibrin activating substances such as glass beads or fluorescent target material separation reagents using magnetic antibody or magnetic microbeads may be added in advance.
  • an anticoagulant such as heparin, ACDA, CPDA, EDTA, or thrombin, calcium divalent ion, Fibrin activating substances such as glass beads or fluorescent target material separation reagents using magnetic antibody or magnetic microbeads may be added in advance.
  • Reference numerals in the above description means those described in FIGS. 1 to 16.
  • Figure 17 is another embodiment of the centrifuge tube of the present invention, when the blood is collected and centrifuged, heavy red blood cell layer is collected in the lower chamber 50, buffy coat layer such as leukocytes and platelets is the intermediate chamber ( 40), the thickness of the intermediate chamber 40 is the diameter of the thinner intermediate chamber 40 in order to prevent the intermediate chamber 40 from being deformed despite the high gravity acceleration (g) generated during centrifugation. It is formed thicker than the other wide upper chamber 30 or lower chamber 50, so that it can withstand high gravity acceleration. In addition, the inlet portion of the upper chamber 30 may be formed to be thick so that the stopper 20 can be bound.
  • the top of the centrifuge tube is a rubber or silicone, polyethylene, polypropylene or PVC is formed of a stopper 20 through which the needle can be penetrated, and the stopper 20 forms a screw thread in the fitting or centrifuge tube. It can be selected from the manner of screwing to form a screw symmetrically to this. That is, by centrifugation of biological liquids such as blood, the liquid such as blood can be injected into the centrifuge tube without opening and closing the stopper 20, so that centrifugal separation can be free from the incorporation of viruses and bacteria. In medical devices or biological devices that require strict sterilization, a closed operation can be performed to prevent contamination of samples, cultures or centrifuges.
  • Figure 18 is another embodiment of the centrifuge tube of the present invention to remove the lower chamber and the intermediate chamber 40 is made to extend to the bottom of the centrifuge tube. It can be selected and used according to the characteristics of the biological solution, and is suitable for centrifugation of a small amount of solution having similar specific gravity. This can be used for more detailed separation, for example, when the composition ratio of red blood cells and platelet leukocytes in the primary separated blood is similar, it can be separated more finely and easily.
  • the support piece 80 formed in the lower portion is formed to extend to the outside to be in close contact with the bucket of the centrifuge to be in close contact with the bucket to prevent vibration during centrifugal separation, by removing from the centrifuge to stand on a flat floor, Help separate each component.
  • the cross-sectional shape of the upper chamber 30 and the lower chamber 50 need not necessarily be a pentagon, and the cross-sectional shape may be an inverted triangle or a triangle as necessary. It can also be shaped like a square.
  • the thickness of the lower chamber 50 by forming a thin and slightly flexible, if the buffy coat layer is caught in the lower chamber 50 by pressing the lower chamber 50, the intermediate chamber 40 by pressing the buffy coat layer Can be easily removed by moving 18 and 20, the support piece 80 formed below the intermediate chamber 40 is formed to be in close contact with the inside of the bucket of the centrifuge to prevent shaking of the centrifuge tube during centrifugation. Playing a role.
  • 21 and 22 are cross-sectional views taken along line B-B and line D-D, respectively.
  • the thickness of the intermediate chamber 40 is thicker than that of the upper chamber 30 so that deformation does not occur even when centrifugation with high gravity acceleration (g) is applied. If the gathered buffy coat layer is formed on the lower chamber 50 side, gently press the lower chamber 50 gently, the buffy coat layer is pushed upward slightly and formed on the intermediate chamber 40 side, so that the needle or capillary tube, etc. It can be separated using. Reference numerals used in the above description means those described in FIGS. 17 to 22.
  • Figure 23 is a perspective view showing one embodiment of the centrifuge tube of the present invention, when the blood and the like is centrifuged, a heavy red blood cell layer is collected in the lower chamber 50, the stem cells such as leukocytes and platelets Buffy coat layer containing is separated into the intermediate chamber 40, plasma components such as serum is to be formed in the upper chamber (30).
  • the surface of a certain height of the upper chamber 30 has a indicator line 100.101 indicating the amount of blood injected into the centrifuge tube or a display portion such as an indicator or an indicator.
  • the leader line 100 indicates the amount of peripheral blood input in the case of a female
  • the leader line 101 indicates the amount of peripheral blood input in the case of a male.
  • the above indicator lines 100 and 101 may not be displayed one by one but may form a quantity indicator line (display scale) as shown in the intermediate chamber in FIGS. 4 and 5 as metering indicator lines. At this time, the volume of each component can be measured and viewed, so that it can be used more conveniently.
  • This display line is shown as being displayed in the intermediate chamber, but may be displayed in the upper chamber and / or the lower chamber. A special male and female mark may be placed on the display line to serve as a support line.
  • a separate plug 60 is formed in the lower chamber 50 to adjust the volume of the lower chamber 50 in a manner of tightening and loosening, resulting in an increase in manufacturing cost.
  • the plug 80 is removed for cleaning and sterilization after reuse for reasons of cost reduction, but the risk of unsanitary use such as reuse of something that is not completely removed from the blood of others stuck in a narrow screw This problem can be solved in the same manner as in this embodiment.
  • the leader lines 100 and 101 indicating the amount of peripheral blood to be introduced are formed on the surface of the upper chamber 30.
  • the leader line (100, 101) is also represented by a thick line to be added differently depending on the sex and the amount of anticoagulant added, such a leader (100, 101) is expressed in a sense of thickness, even if the same leader (100, 101) A positive dose of anticoagulant may be indicated, and the bottom of the line may indicate that a high dose of anticoagulant is applied.
  • leader lines 100 and 101 may be marked separately from the scale indicating the total volume, or may be marked like the scale indicating the total volume.
  • the indicators 100 and 101 may be displayed differently depending on other separation materials other than the peripheral blood of a human being injected into the centrifuge tube.
  • the top of the centrifuge tube is formed with a stopper 20 through which the needle can be penetrated as a chemical polymer material such as rubber, silicone, polyethylene, polypropylene or PVC, the stopper 20 is fitted It can be selected from the way of forming a screw thread on the centrifuge tube and symmetrically screwed and screwed in, and the stopper 20 has a slightly raised shape at the top so that it can be wiped with an alcohol swab before inserting the needle.
  • the cap 90 was formed to be well wiped without opening, and the inside thereof was formed concave conversely. This allows the collection of certain materials inside the centrifuge tubes in concave areas during collection.
  • a cap 90 having an upper portion formed separately from the cap 20 is formed so that the cap 20 can be prevented from opening when the needle inserted into the cap 20 is pulled out again. ) May be formed.
  • the cap 90 forms a binding groove 81 on its inner surface and is engaged with the binding jaw 80 in the centrifuge tube corresponding thereto. Since it is formed in this way, even without opening and closing the stopper 20 or the cap 90, the peripheral blood is injected into the centrifuge tube, and the separation and extraction of the desired components can be prevented, thereby preventing the incorporation of viruses or bacteria. It is effective and can be closed in medical devices or biological devices that require strict sterilization to prevent contamination of samples, cultures or centrifuges.
  • the binding jaw 80 and the binding groove 81 may be formed of a screw line or the like, of course.
  • the thickness of the lower chamber 50 slightly more flexible than the thickness of the other two chambers, if the buffy coat layer is hung on the lower chamber 50, the flexible portion of the bottom surface of the lower chamber 50 Press to move the Buffy coat layer toward the intermediate chamber 40 can be easily separated.
  • a support piece may be formed on the surface of the intermediate chamber 40 having a small diameter.
  • the needle end may be inserted into the buffy coat layer and sucked out after injection.
  • the last part of the buffy coat layer is bounded by a thin layer of red blood cells at the bottom.
  • the needle tip is rotated as a circle into the middle chamber wall, the relatively light buffy coat layer is attached to the middle chamber wall by centrifugal force.
  • the silver is collected at the center.
  • the remaining minute amount of the buffy coat layer can be collected.
  • the surface 110 of the centrifuge tube is formed with a display surface 110 on which the name of the subject, the test date, and the like can be recorded. Due to the display surface 110, when many tests are made at the same time, the inspector can prevent misunderstandings due to the chaos of the sample.
  • FIG. 26 is a cross-sectional view taken along line AA ′ of FIG. 25, wherein a binding jaw 80 is formed on the surface of the centrifuge tube and a binding groove 81 is formed on the inner surface of the cap 90 at a position corresponding thereto. It shows a binding.
  • Reference numerals used in the above description means those described in FIGS. 23 to 26.
  • the present invention also adds to the centrifuge tube a water-soluble high molecular compound or sedimentation material such as Ficoll-Hypaque to precipitate a specific substance, or anticoagulant or thrombin such as heparin, ACDA, CPDA, EDTA, Fibrin activating materials such as calcium divalent ions, glass beads, or fluorescent target separation agent or magnetic microbeads using antigen-antibody reactions are pre-injected so that they can be conveniently used for necessary applications.
  • a water-soluble high molecular compound or sedimentation material such as Ficoll-Hypaque to precipitate a specific substance, or anticoagulant or thrombin such as heparin, ACDA, CPDA, EDTA, Fibrin activating materials such as calcium divalent ions, glass beads, or fluorescent target separation agent or magnetic microbeads using antigen-antibody reactions are pre-injected so that they can be conveniently used for necessary applications.
  • the present invention is used in medical biotechnology by centrifuging blood to separate specific components and using the separated components. Apparatuses of the present invention assist in the isolation, cultivation and regeneration of cells in an economical, safe and efficient manner in the separation of biologically contaminated biological materials and the like, which occur during their separation.

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Abstract

La présente invention concerne un tube pour centrifugeuse destiné à être utilisé dans la centrifugation de matériaux biologiques, le diamètre du tube pour centrifugeuse étant amélioré pour permettre une centrifugation minute, et dans lequel une partie fine, faible, et étroite du tube pour centrifugeuse, sur laquelle doit être appliquée une interaction forte, comprend une pièce de support permettant la réalisation de la centrifugation sans provoquer de déformation dans le tube pour centrifugeuse. Le tube pour centrifugeuse de la présente invention permet de réaliser facilement la centrifugation de matériaux en utilisant des différences dans les densités des matériaux sans utiliser de réactif ou équivalents. Le tube pour centrifugeuse de la présente invention comprend un dispositif de fermeture fabriqué à partir d'un matériau tel qu'un caoutchouc, du polyéthylène, du polypropylène, du silicium ou équivalents, de sorte qu'une aiguille puisse pénétrer à travers le dispositif de fermeture et le tube pour centrifugeuse peut être protégé d'une contamination provoquée par des bactéries, et la centrifugation de matériaux biologiques peut être réalisée dans un espace fermé.
PCT/KR2010/008968 2009-12-07 2010-12-15 Tube pour centrifugeuse WO2011071353A2 (fr)

Priority Applications (5)

Application Number Priority Date Filing Date Title
EP10836248.4A EP2511011A4 (fr) 2009-12-07 2010-12-15 Tube pour centrifugeuse
US13/513,906 US20120251411A1 (en) 2009-12-07 2010-12-15 Centrifuge tube
KR1020127012529A KR101289535B1 (ko) 2009-12-07 2010-12-15 원심분리관
JP2012543030A JP2013514874A (ja) 2009-12-19 2010-12-15 遠心分離管
CN2010800554604A CN102844121A (zh) 2009-12-19 2010-12-15 离心分离管

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KR20090120282 2009-12-07
KR10-2009-0120282 2009-12-07
KR20090127565 2009-12-19
KR10-2009-0127565 2009-12-19
KR10-2010-0004358 2010-01-18
KR20100004358 2010-01-18

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WO2011071353A3 WO2011071353A3 (fr) 2011-11-10

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CN103566990A (zh) * 2012-07-20 2014-02-12 群祐生技股份有限公司 离心管结构
WO2014036945A1 (fr) * 2012-09-10 2014-03-13 Yang Chaocheng Structure de tube de centrifugeuse
CN103732269A (zh) * 2011-06-19 2014-04-16 瓦尔特·波比奇卡 用于分离血液的方法、用于血液离心分离机的分离容器和用于填充冷冻容器的系统
US20140206521A1 (en) * 2013-01-18 2014-07-24 Ziad Hamandi Centrifugal separating assembly
CN103958069A (zh) * 2011-12-06 2014-07-30 李範夏 离心分离器用容器
CN104010672A (zh) * 2011-12-20 2014-08-27 瑞崴制造股份有限公司 血液分离装置
WO2021224614A1 (fr) * 2020-05-05 2021-11-11 Micromass Uk Ltd Capillaire

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CN103732269A (zh) * 2011-06-19 2014-04-16 瓦尔特·波比奇卡 用于分离血液的方法、用于血液离心分离机的分离容器和用于填充冷冻容器的系统
CN103111097A (zh) * 2011-11-16 2013-05-22 玛旺干细胞医学生物科技股份有限公司 富含血小板血浆采集装置
CN103958069A (zh) * 2011-12-06 2014-07-30 李範夏 离心分离器用容器
JP2015502851A (ja) * 2011-12-06 2015-01-29 バム ハ リー， 遠心分離機用容器
CN104010672A (zh) * 2011-12-20 2014-08-27 瑞崴制造股份有限公司 血液分离装置
CN103566990A (zh) * 2012-07-20 2014-02-12 群祐生技股份有限公司 离心管结构
WO2014036945A1 (fr) * 2012-09-10 2014-03-13 Yang Chaocheng Structure de tube de centrifugeuse
US20140206521A1 (en) * 2013-01-18 2014-07-24 Ziad Hamandi Centrifugal separating assembly
US9610590B2 (en) * 2013-01-18 2017-04-04 Ziad Hamandi Centrifugal separating assembly with a container body having a common inlet-outlet port
WO2021224614A1 (fr) * 2020-05-05 2021-11-11 Micromass Uk Ltd Capillaire

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KR101289535B1 (ko) 2013-07-24
EP2511011A4 (fr) 2014-07-09
EP2511011A2 (fr) 2012-10-17
US20120251411A1 (en) 2012-10-04
KR20120089723A (ko) 2012-08-13
WO2011071353A3 (fr) 2011-11-10

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