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WO2011066304A3 - Engineered beta-class carbonic anhydrase polypeptides and uses thereof - Google Patents

Engineered beta-class carbonic anhydrase polypeptides and uses thereof Download PDF

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Publication number
WO2011066304A3
WO2011066304A3 PCT/US2010/057843 US2010057843W WO2011066304A3 WO 2011066304 A3 WO2011066304 A3 WO 2011066304A3 US 2010057843 W US2010057843 W US 2010057843W WO 2011066304 A3 WO2011066304 A3 WO 2011066304A3
Authority
WO
WIPO (PCT)
Prior art keywords
carbonic anhydrase
engineered
class carbonic
class
anhydrase polypeptides
Prior art date
Application number
PCT/US2010/057843
Other languages
French (fr)
Other versions
WO2011066304A2 (en
Inventor
Louis Clark
Sabrina Zimmerman
Original Assignee
Codexis, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Codexis, Inc. filed Critical Codexis, Inc.
Publication of WO2011066304A2 publication Critical patent/WO2011066304A2/en
Publication of WO2011066304A3 publication Critical patent/WO2011066304A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P3/00—Preparation of elements or inorganic compounds except carbon dioxide

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

The present disclosure relates to engineered β-class carbonic anhydrase enzymes having improved properties as compared to a naturally occurring wild type β-class carbonic anhydrase and uses thereof for the sequestration of carbon dioxide as well as for the release of carbon dioxide from a composition comprising bicarbonate. Also provided are polynucleotides encoding the engineered carbonic anhydrase enzymes and host cells capable of expressing the engineered carbonic anhydrase enzymes.
PCT/US2010/057843 2009-11-25 2010-11-23 Engineered beta-class carbonic anhydrase polypeptides and uses thereof WO2011066304A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US26460009P 2009-11-25 2009-11-25
US61/264,600 2009-11-25

Publications (2)

Publication Number Publication Date
WO2011066304A2 WO2011066304A2 (en) 2011-06-03
WO2011066304A3 true WO2011066304A3 (en) 2012-01-12

Family

ID=44067199

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2010/057843 WO2011066304A2 (en) 2009-11-25 2010-11-23 Engineered beta-class carbonic anhydrase polypeptides and uses thereof

Country Status (1)

Country Link
WO (1) WO2011066304A2 (en)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MX2012002532A (en) 2009-09-01 2012-04-11 Novozymes Inc Methods for improving malic acid production in filamentous fungi.
US8617859B2 (en) 2010-06-04 2013-12-31 Novozymes, Inc. C4 dicarboxylic acid production in filamentous fungi
CN102947458B (en) 2010-06-21 2016-05-04 诺维信股份有限公司 Be used for the method for the generation of the improvement of filamentous fungi C4-dicarboxylic acids
JP5912111B2 (en) 2010-06-21 2016-04-27 ノボザイムス，インコーポレイティド Polypeptide having C4-dicarboxylic acid transporter activity derived from Aspergillus acreatus and polynucleotide encoding the same
CA2803952C (en) 2010-06-30 2020-03-24 Codexis, Inc. Highly stable beta-class carbonic anhydrases useful in carbon capture systems
US8354261B2 (en) 2010-06-30 2013-01-15 Codexis, Inc. Highly stable β-class carbonic anhydrases useful in carbon capture systems
WO2012003336A2 (en) 2010-06-30 2012-01-05 Codexis, Inc. Chemically modified carbonic anhydrases useful in carbon capture systems
US8722387B2 (en) 2011-02-28 2014-05-13 Novozymes, Inc. Microorganisms for C4-dicarboxylic acid production
US8663956B2 (en) 2011-08-19 2014-03-04 Novozymes, Inc. Recombinant microorganisms for production C4-dicarboxylic acids
WO2014090327A1 (en) * 2012-12-14 2014-06-19 Statoil Petoleum As Novel enzymes for enhanced gas absorption
US10415028B2 (en) * 2015-09-03 2019-09-17 Co2 Solutions Inc. Variants of thermovibrio ammonificans carbonic anhydrase and CO2 capture methods using thermovibrio ammonificans carbonic anhydrase variants
BR102018014255A2 (en) * 2018-07-12 2020-01-28 Leonardo Garnica reverse breathing process for carbon dioxide absorption

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060257990A1 (en) * 2005-04-21 2006-11-16 Co2 Solution Carbonic anhydrase having increased stability under high temperature conditions
WO2008095057A2 (en) * 2007-01-31 2008-08-07 Novozymes A/S Heat-stable carbonic anhydrases and their use
US20100209997A1 (en) * 2009-01-09 2010-08-19 Codexis, Inc. Carbonic anhydrase polypeptides and uses thereof

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060257990A1 (en) * 2005-04-21 2006-11-16 Co2 Solution Carbonic anhydrase having increased stability under high temperature conditions
WO2008095057A2 (en) * 2007-01-31 2008-08-07 Novozymes A/S Heat-stable carbonic anhydrases and their use
US20100209997A1 (en) * 2009-01-09 2010-08-19 Codexis, Inc. Carbonic anhydrase polypeptides and uses thereof

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
STROP, P. ET AL.: "Crystal structure of the ''cab''-type beta class carbonic anhydrase from the archaeon methanobacterium thermoautotrophicum", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 276, no. 13, 28 November 2000 (2000-11-28), pages 10299 - 10305 *

Also Published As

Publication number Publication date
WO2011066304A2 (en) 2011-06-03

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