WO2010093150A3 - Recombinant microorganism with enhanced ability for glycerol metabolism and succinic acid production, and method for producing succinic acid using same - Google Patents
Recombinant microorganism with enhanced ability for glycerol metabolism and succinic acid production, and method for producing succinic acid using same Download PDFInfo
- Publication number
- WO2010093150A3 WO2010093150A3 PCT/KR2010/000755 KR2010000755W WO2010093150A3 WO 2010093150 A3 WO2010093150 A3 WO 2010093150A3 KR 2010000755 W KR2010000755 W KR 2010000755W WO 2010093150 A3 WO2010093150 A3 WO 2010093150A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- succinic acid
- recombinant microorganism
- acid
- present
- producing
- Prior art date
Links
- KDYFGRWQOYBRFD-UHFFFAOYSA-N succinic acid Chemical compound OC(=O)CCC(O)=O KDYFGRWQOYBRFD-UHFFFAOYSA-N 0.000 title abstract 13
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 title abstract 9
- 239000001384 succinic acid Substances 0.000 title abstract 6
- 244000005700 microbiome Species 0.000 title abstract 5
- 238000004519 manufacturing process Methods 0.000 title abstract 4
- 230000004060 metabolic process Effects 0.000 title abstract 2
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 abstract 3
- LCTONWCANYUPML-UHFFFAOYSA-N Pyruvic acid Chemical compound CC(=O)C(O)=O LCTONWCANYUPML-UHFFFAOYSA-N 0.000 abstract 2
- 239000006227 byproduct Substances 0.000 abstract 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 abstract 2
- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 abstract 2
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 abstract 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 abstract 1
- 235000011054 acetic acid Nutrition 0.000 abstract 1
- 230000031018 biological processes and functions Effects 0.000 abstract 1
- KDYFGRWQOYBRFD-NUQCWPJISA-N butanedioic acid Chemical compound O[14C](=O)CC[14C](O)=O KDYFGRWQOYBRFD-NUQCWPJISA-N 0.000 abstract 1
- 229910052799 carbon Inorganic materials 0.000 abstract 1
- 238000001311 chemical methods and process Methods 0.000 abstract 1
- 238000007796 conventional method Methods 0.000 abstract 1
- 235000019253 formic acid Nutrition 0.000 abstract 1
- 239000004310 lactic acid Substances 0.000 abstract 1
- 235000014655 lactic acid Nutrition 0.000 abstract 1
- 239000002207 metabolite Substances 0.000 abstract 1
- 229940107700 pyruvic acid Drugs 0.000 abstract 1
- 239000000126 substance Substances 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
- C12P7/44—Polycarboxylic acids
- C12P7/46—Dicarboxylic acids having four or less carbon atoms, e.g. fumaric acid, maleic acid
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Landscapes
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Physics & Mathematics (AREA)
- General Chemical & Material Sciences (AREA)
- Plant Pathology (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The present invention relates to a recombinant microorganism with enhanced ability for glycerol metabolism and succinic acid production, and to a method for producing succinic acid using same. More particularly, the present invention relates to a recombinant microorganism which is enhanced to produce a high concentration succinic acid by effectively using inexpensive glycerol as a carbon source for cells. The recombinant microorganism according to the present invention enables succinic acid, which is a valuable substance produced by conventional chemical processes, to be produced through production-efficient and environmentally-friendly biological processes, thus reducing the costs of producing the target metabolite. Further, the recombinant microorganism according to the present invention is advantageous in that 100% pure succinic acid can be produced without forming byproducts, as opposed to conventional techniques in which only a slight reduction of byproducts such as acetic acid, lactic acid, formic acid, pyruvic acid, etc., is achieved.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020090011453A KR101093199B1 (en) | 2009-02-12 | 2009-02-12 | Recombinant Microorganism Having Enhanced Glycerol Metabolism and Succinic Acid-Productivity and Method for Preparing Succinic Acid Using the Same |
| KR10-2009-0011453 | 2009-02-12 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| WO2010093150A2 WO2010093150A2 (en) | 2010-08-19 |
| WO2010093150A3 true WO2010093150A3 (en) | 2010-12-09 |
Family
ID=42562166
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/KR2010/000755 WO2010093150A2 (en) | 2009-02-12 | 2010-02-08 | Recombinant microorganism with enhanced ability for glycerol metabolism and succinic acid production, and method for producing succinic acid using same |
Country Status (2)
| Country | Link |
|---|---|
| KR (1) | KR101093199B1 (en) |
| WO (1) | WO2010093150A2 (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101221557B1 (en) * | 2010-08-30 | 2013-01-14 | 한국과학기술원 | Novel Engineered Microorganism Producing Succinic Acid with High Yield by Utilizing Both Sucrose and Glycerol at the Same Time and Method for Preparing Succinic Acid Using the Same |
| CN103842495B (en) * | 2011-07-22 | 2018-04-13 | 麦兰特公司 | Glycerol fermentation is organic acid |
| KR102129379B1 (en) * | 2018-10-10 | 2020-07-02 | 한국과학기술원 | A recombinant microorganism into which a high activity malate dehydrogenase for producing succinic acid and a method for producing succinic acid using the same |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2005052135A1 (en) * | 2003-11-27 | 2005-06-09 | Korea Advanced Institute Of Science And Technology | Novel rumen bacteria variants and process for preparing succinic acid employing the same |
| WO2007115228A2 (en) * | 2006-03-31 | 2007-10-11 | Rice University | Anaerobic fermentation of glycerol |
-
2009
- 2009-02-12 KR KR1020090011453A patent/KR101093199B1/en active Active
-
2010
- 2010-02-08 WO PCT/KR2010/000755 patent/WO2010093150A2/en active Application Filing
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2005052135A1 (en) * | 2003-11-27 | 2005-06-09 | Korea Advanced Institute Of Science And Technology | Novel rumen bacteria variants and process for preparing succinic acid employing the same |
| WO2007115228A2 (en) * | 2006-03-31 | 2007-10-11 | Rice University | Anaerobic fermentation of glycerol |
Non-Patent Citations (2)
| Title |
|---|
| RITTMANN, D. ET AL.: "Engineering of a Glycerol Utilization Pathway for Amino Acid Production by Corynebacterium Glutamicum", APPL. ENVIRON. MICROBIOL., vol. 74, no. 20, October 2008 (2008-10-01), pages 6216 - 6222 * |
| ZHAO, L. ET AL.: "Effects of Over-Expression of Glycerol Dehydrogenase and 1,3- Propanediol Oxidoreductase on Bioconversion of Glycerol into 1,3-Propandediol by Klebsiella pneumoniae under Micro-Aerobic Conditions", BIOPROCESS BIOSYST. ENG., vol. 32, no. 3, August 2008 (2008-08-01), pages 313 - 320 * |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2010093150A2 (en) | 2010-08-19 |
| KR101093199B1 (en) | 2011-12-12 |
| KR20100092190A (en) | 2010-08-20 |
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