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WO2009126927A3 - Procédés de génération de cellules produisant de l'insuline - Google Patents

Procédés de génération de cellules produisant de l'insuline Download PDF

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Publication number
WO2009126927A3
WO2009126927A3 PCT/US2009/040266 US2009040266W WO2009126927A3 WO 2009126927 A3 WO2009126927 A3 WO 2009126927A3 US 2009040266 W US2009040266 W US 2009040266W WO 2009126927 A3 WO2009126927 A3 WO 2009126927A3
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WO
WIPO (PCT)
Prior art keywords
cells
population
insulin
methods
producing cells
Prior art date
Application number
PCT/US2009/040266
Other languages
English (en)
Other versions
WO2009126927A2 (fr
Inventor
Gordon Weir
Masaki Nagaya
Original Assignee
Joslin Diabetes Center, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Joslin Diabetes Center, Inc. filed Critical Joslin Diabetes Center, Inc.
Publication of WO2009126927A2 publication Critical patent/WO2009126927A2/fr
Publication of WO2009126927A3 publication Critical patent/WO2009126927A3/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0676—Pancreatic cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K2035/126—Immunoprotecting barriers, e.g. jackets, diffusion chambers
    • A61K2035/128—Immunoprotecting barriers, e.g. jackets, diffusion chambers capsules, e.g. microcapsules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/01—Modulators of cAMP or cGMP, e.g. non-hydrolysable analogs, phosphodiesterase inhibitors, cholera toxin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/105—Insulin-like growth factors [IGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/11—Epidermal growth factor [EGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/12—Hepatocyte growth factor [HGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/335—Glucagon; Glucagon-like peptide [GLP]; Exendin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/42—Notch; Delta; Jagged; Serrate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/14—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from hepatocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/70—Polysaccharides
    • C12N2533/74—Alginate

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  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

L'invention concerne un procédé de génération d'une population de cellules ayant des cellules produisant de l'insuline, comprenant l'obtention d'une population de cellules épithéliales biliaires intrahépatiques; l'agrandissement de la population de cellules; la mise en culture de la population de cellules agrandie dans un milieu manquant d'insuline et du sérum; et la mise en contact de la population de cellules avec un état de différenciation pendant un temps suffisant pour qu'au moins une partie des cellules sécrètent de l'insuline.
PCT/US2009/040266 2008-04-11 2009-04-10 Procédés de génération de cellules produisant de l'insuline WO2009126927A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US12396808P 2008-04-11 2008-04-11
US61/123,968 2008-04-11

Publications (2)

Publication Number Publication Date
WO2009126927A2 WO2009126927A2 (fr) 2009-10-15
WO2009126927A3 true WO2009126927A3 (fr) 2010-02-25

Family

ID=41162664

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2009/040266 WO2009126927A2 (fr) 2008-04-11 2009-04-10 Procédés de génération de cellules produisant de l'insuline

Country Status (1)

Country Link
WO (1) WO2009126927A2 (fr)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8835400B2 (en) 2010-10-08 2014-09-16 Mina Therapeutics Limited RNA molecules that upregulate insulin production
WO2012168434A1 (fr) * 2011-06-08 2012-12-13 INSERM (Institut National de la Santé et de la Recherche Médicale) Reprogrammation partielle de cellules somatiques en cellules souches tissulaires induites (its)
JP6650393B2 (ja) 2013-06-13 2020-02-19 オージェネシス リミテッド 細胞の集団、分化転換の方法及びその使用方法
CA3236835A1 (fr) 2013-11-22 2015-05-28 Mina Therapeutics Limited Compositions d'arn a activation courte de c/ebp alpha et methodes d'utilisation
EP2975116B1 (fr) 2014-07-16 2019-08-21 Max-Delbrück-Centrum für Molekulare Medizin Reprogrammation induite par Tgif2 des cellules hépatiques en cellules progénitrices pancréatiques et leurs utilisations médicales
MA41296A (fr) * 2014-12-30 2017-11-07 Orgenesis Ltd Procédés de transdifférenciation et procédés d'utilisation de ceux-ci
DK3286300T3 (da) 2015-04-24 2021-01-18 Univ Copenhagen Isolering af bona fide pankreatiske stamceller
SG11201911256UA (en) 2017-05-08 2020-01-30 Orgenesis Ltd Transdifferentiated cell populations and methods of use thereof
CA2983845C (fr) 2017-10-26 2024-01-30 University Of Copenhagen Generation de cellules beta reagissant au glucose

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
KANETO, H. ET AL.: "PDX-1/VP16 fusion protein, together with NeuroD or Ngn3, markedly induces insulin gene transcription and ameliorates glucose tolerance.", DIABETES., vol. 54, no. 4, April 2005 (2005-04-01), pages 1009 - 1022 *
SUMAZAKI, R. ET AL.: "Conversion of biliary system to pancreatic tissue in Hes1-deficient mice.", NATURE GENETICS., vol. 36, no. 1, 14 December 2003 (2003-12-14), pages 83 - 87 *
TANG, D. Q.: "Reprogramming liver-stem WB cells into functional insulin-producing cells by persistent expression of Pdxl- and Pdxl-VP16 mediated by lentiviral vectors.", LABORATORY INVESTIGATION., vol. 86, no. 1, January 2006 (2006-01-01), pages 83 - 93 *
WILDING, L. ET AL.: "The role of pdx and HNF6 in proliferation and differentiation of endocrine precursors.", DIABETES/METABOLISM RESEARCH AND REVIEWS., vol. 20, no. 2, March 2004 (2004-03-01), pages 114 - 123 *
YAMADA, S.: "In vitro transdifferentiation of mature hepatocytes into insulin-producing cells.", ENDOCRINE JOURNAL., vol. 53, no. 6, 19 September 2006 (2006-09-19), pages 789 - 795 *

Also Published As

Publication number Publication date
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