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WO2009108693A3 - Composition and methods for rapid detection of hiv by loop- mediated isothermal amplification (lamp) - Google Patents

Composition and methods for rapid detection of hiv by loop- mediated isothermal amplification (lamp) Download PDF

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Publication number
WO2009108693A3
WO2009108693A3 PCT/US2009/035130 US2009035130W WO2009108693A3 WO 2009108693 A3 WO2009108693 A3 WO 2009108693A3 US 2009035130 W US2009035130 W US 2009035130W WO 2009108693 A3 WO2009108693 A3 WO 2009108693A3
Authority
WO
WIPO (PCT)
Prior art keywords
hiv
lamp
nucleic acids
methods
loop
Prior art date
Application number
PCT/US2009/035130
Other languages
French (fr)
Other versions
WO2009108693A2 (en
Inventor
Michele S. Owen
Kelly Curtis
Donna Rudolph
Original Assignee
The Government Of The United States Of America , As Represented By The Secretary, Department Of Health And Human Services,
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by The Government Of The United States Of America , As Represented By The Secretary, Department Of Health And Human Services, filed Critical The Government Of The United States Of America , As Represented By The Secretary, Department Of Health And Human Services,
Priority to US12/918,536 priority Critical patent/US8900807B2/en
Publication of WO2009108693A2 publication Critical patent/WO2009108693A2/en
Publication of WO2009108693A3 publication Critical patent/WO2009108693A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/702—Specific hybridization probes for retroviruses
    • C12Q1/703—Viruses associated with AIDS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/119—Strand displacement amplification [SDA]

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Virology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • AIDS & HIV (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Methods and compositions for detection of HIV nucleic acids in a sample, such as a biological sample obtained from a human subject, are provided according to embodiments of the present invention which include providing a reaction mixture including at least one LAMP, accelerated LAMP, RT-LAMP or RT-accelerated LAMP assay primer set specific for HIV-I or HIV-2 nucleic acids and the biological sample to be tested for presence of HIV-I and/or HIV-2 nucleic acids; incubating the reaction mixture under conditions suitable to produce a LAMP assay reaction product; and detecting the reaction product. Primers and primer sets for use in LAMP assays of HIV-I or HIV-2 nucleic acids are provided.
PCT/US2009/035130 2008-02-25 2009-02-25 Composition and methods for rapid detection of hiv by loop- mediated isothermal amplification (lamp) WO2009108693A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US12/918,536 US8900807B2 (en) 2008-02-25 2009-02-25 Composition and methods for rapid detection of HIV by loop-mediated isothermal amplification (LAMP)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US3112808P 2008-02-25 2008-02-25
US61/031,128 2008-02-25

Publications (2)

Publication Number Publication Date
WO2009108693A2 WO2009108693A2 (en) 2009-09-03
WO2009108693A3 true WO2009108693A3 (en) 2009-12-23

Family

ID=41016690

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2009/035130 WO2009108693A2 (en) 2008-02-25 2009-02-25 Composition and methods for rapid detection of hiv by loop- mediated isothermal amplification (lamp)

Country Status (2)

Country Link
US (1) US8900807B2 (en)
WO (1) WO2009108693A2 (en)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
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US20130171643A1 (en) * 2010-06-22 2013-07-04 University Of Hawaii Sequence Specific Real-Time Monitoring of Loop-Mediated Isothermal Amplification (LAMP)
WO2012108961A1 (en) * 2011-02-09 2012-08-16 The United States Of America As Represented By The Secretary Of The Navy Detection of hepatitis b virus in blood using lamp method
US9074249B2 (en) 2012-06-04 2015-07-07 New England Biolabs, Inc. Detection of amplification products
US9074243B2 (en) 2012-07-27 2015-07-07 New England Biolabs, Inc. Detection of amplification products
TWI600766B (en) 2012-08-09 2017-10-01 財團法人工業技術研究院 Kit for detecting a mutation and/or polymorphism of a specific region in a target nucleotide sequence
GB201304797D0 (en) * 2013-03-15 2013-05-01 Diagnostics For The Real World Ltd Apparatus and method for automated sample preparation and adaptor for use in the apparatus
GB201319180D0 (en) * 2013-10-30 2013-12-11 Mast Group Ltd Nucleic acid probe
WO2015118491A1 (en) * 2014-02-06 2015-08-13 University Of The Witwatersrand, Johannesburg Method for detecting hiv
WO2015127201A1 (en) 2014-02-21 2015-08-27 The United States Of America, As Represented By The Secretary, Department Of Health & Human Services Hiv-2 nucleic acids and methods of detection
WO2015160536A1 (en) * 2014-04-14 2015-10-22 The United States Of America, As Represented By The Secretary Department Of Health And Human Services Methods for rapid detection and identification of viral nucleic acids
EP3215639B1 (en) 2014-11-06 2019-05-15 The United States of America, as represented by The Secretary, Department of Health and Human Services Detection of hiv-1 nucleic acids by reverse-transcription loop-mediated isothermal amplication
US10731227B2 (en) * 2014-12-12 2020-08-04 The Trustees Of The University Of Pennsylvania Compositions, kits, and methods to detect HIV virus
US10724091B1 (en) 2015-02-10 2020-07-28 National Technology & Engineering Solutions Of Sandia, Llc Endpoint detection of amplified nucleic acids
US11091745B2 (en) 2015-05-12 2021-08-17 Dna Polymerase Technology, Inc. Mutant polymerases and uses thereof
US11366116B1 (en) 2017-04-12 2022-06-21 National Technology & Engineering Solutions Of Sandia, Llc Real time autonomous surveillance of pathogens
KR101899371B1 (en) * 2017-07-25 2018-10-29 (주)엔바이오텍 Nucleic Acid Complex Pair, PCR Kit Comprising Nucleic Acid Complex Pair, and Target Detection Method Using Nucleic Acid Complex Pair

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US20070196902A1 (en) 2006-01-31 2007-08-23 University Of Pittsburgh Method of detecting AZT resistance in HIV
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WO2012108961A1 (en) * 2011-02-09 2012-08-16 The United States Of America As Represented By The Secretary Of The Navy Detection of hepatitis b virus in blood using lamp method

Non-Patent Citations (4)

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Title
CURTIS K. ET AL.: "Rapid detection ofHIV-1 by reverse-transcription, loop-mediated isothermal amplification (RT-LAMP)", JOURNAL OF VIROLOGICAL METHODS, vol. 151, no. 2, August 2008 (2008-08-01), pages 264 - 270 *
HOSAKA K. ET AL.: "Rapid detection of human immunodeficiency virus type 1 group M by a reverse transcription-loop-mediated isothermal amplification assay", JOURNAL OF VIROLOGICAL METHODS, vol. 157, no. 2, May 2009 (2009-05-01), pages 195 - 199 *
LEE S. ET AL.: "Efficient, specific, compact hepatitis B diagnostic device: Optical detection of the hepatitis B virus by isothermal amplification", SENSORS AND ACTUATORS B: CHEMICAL, vol. 127, no. 2, 15 November 2007 (2007-11-15), pages 598 - 605 *
THAI H. ET AL.: "Development and Evaluation of a Novel Loop-Mediated Isothermal Amplification Method for Rapid Detection of Severe Acute Respiratory Syndrome Coronavirus", JOURNAL OF CLINICAL MICROBIOLOGY, vol. 42, no. 5, May 2004 (2004-05-01), pages 1956 - 1961 *

Also Published As

Publication number Publication date
US20120088244A1 (en) 2012-04-12
WO2009108693A2 (en) 2009-09-03
US8900807B2 (en) 2014-12-02

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