WO2009035770A3 - Cholera toxin subunit b conjugated quantum dots for live cell labelling - Google Patents
Cholera toxin subunit b conjugated quantum dots for live cell labelling Download PDFInfo
- Publication number
- WO2009035770A3 WO2009035770A3 PCT/US2008/070872 US2008070872W WO2009035770A3 WO 2009035770 A3 WO2009035770 A3 WO 2009035770A3 US 2008070872 W US2008070872 W US 2008070872W WO 2009035770 A3 WO2009035770 A3 WO 2009035770A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- labelling
- conjugates
- quantum dots
- cholera toxin
- live cell
- Prior art date
Links
- 238000002372 labelling Methods 0.000 title abstract 5
- 239000002096 quantum dot Substances 0.000 title abstract 2
- 102000009016 Cholera Toxin Human genes 0.000 title 1
- 108010049048 Cholera Toxin Proteins 0.000 title 1
- 210000004027 cell Anatomy 0.000 abstract 5
- 150000002270 gangliosides Chemical class 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 2
- 241000607626 Vibrio cholerae Species 0.000 abstract 1
- 239000003153 chemical reaction reagent Substances 0.000 abstract 1
- 210000000805 cytoplasm Anatomy 0.000 abstract 1
- 210000004962 mammalian cell Anatomy 0.000 abstract 1
- 239000003053 toxin Substances 0.000 abstract 1
- 231100000765 toxin Toxicity 0.000 abstract 1
- 229940118696 vibrio cholerae Drugs 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/28—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Vibrionaceae (F)
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y15/00—Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y5/00—Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/588—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with semiconductor nanocrystal label, e.g. quantum dots
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
- G01N2333/28—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Vibrionaceae (F)
Landscapes
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Nanotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Crystallography & Structural Chemistry (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Urology & Nephrology (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Biochemistry (AREA)
- Hematology (AREA)
- Gastroenterology & Hepatology (AREA)
- General Physics & Mathematics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Materials Engineering (AREA)
- Microbiology (AREA)
- Genetics & Genomics (AREA)
- Food Science & Technology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Cell Biology (AREA)
- Pathology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Medical Informatics (AREA)
- Pharmacology & Pharmacy (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
Conjugates were developed for internal labelling and tracking of live cells having cell surface gangliosides. The conjugate is comprised of at least one, and preferably up to three Vibrio cholerae toxin subunits B, preferably in the pentameric form, attached to a quantum dot. The conjugates may be used as luminescent labels in a reagent system for labelling live cells. The conjugates are internalized into small vesicles dispersed throughout the cytoplasm. A method of labelling and method of detecting cells having cell surface gangliosides are described. The conjugates are particularly useful for the general labelling of mammalian cells.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US96163507P | 2007-07-23 | 2007-07-23 | |
US60/961,635 | 2007-07-23 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2009035770A2 WO2009035770A2 (en) | 2009-03-19 |
WO2009035770A3 true WO2009035770A3 (en) | 2009-05-14 |
Family
ID=40382083
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2008/070872 WO2009035770A2 (en) | 2007-07-23 | 2008-07-23 | Cholera toxin subunit b conjugated quantum dots for live cell labelling |
Country Status (1)
Country | Link |
---|---|
WO (1) | WO2009035770A2 (en) |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2010099796A2 (en) | 2009-03-04 | 2010-09-10 | Medotech A/S | Apparatus for electrical stimulation, in particular for bruxism |
KR20140106550A (en) * | 2011-11-30 | 2014-09-03 | 에이전시 포 사이언스, 테크놀로지 앤드 리서치 | GM1 ganglioside to Annexin V microparticle polypeptide ratio for biological monitoring |
CN109293754A (en) * | 2018-09-29 | 2019-02-01 | 郑州大学第附属医院 | Novel red fluorescent nano-neural tracer |
-
2008
- 2008-07-23 WO PCT/US2008/070872 patent/WO2009035770A2/en active Application Filing
Non-Patent Citations (10)
Title |
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CHAKRABORTY SUBHASISH K ET AL: "Cholera toxin B conjugated quantum dots for live cell labeling", NANO LETTERS, ACS, WASHINGTON, DC, US, vol. 7, no. 9, 31 July 2007 (2007-07-31), pages 2618 - 2626, XP009102072, ISSN: 1530-6984 * |
DERFUS A M ET AL: "Intracellular delivery of quantum dots for live cell labeling and organelle tracking", ADVANCED MATERIALS 20040617 WILEY-VCH VERLAG DE, vol. 16, no. 12, 17 June 2004 (2004-06-17), pages 961 - 966, XP002518316 * |
FUJINAGA YUKAKO ET AL: "Gangliosides that associate with lipid rafts mediate transport of cholera and related toxins from the plasma membrane to endoplasmic reticulm.", MOLECULAR BIOLOGY OF THE CELL DEC 2003, vol. 14, no. 12, December 2003 (2003-12-01), pages 4783 - 4793, XP002518312, ISSN: 1059-1524 * |
HOTZ CHARLES Z ED - ROSENTHAL SANDRA J ET AL: "Applications of quantum dots in biology: an overview", NANOBIOTECHNOLOGY PROTOCOLS, HUMANA PRESS, UNITED STATES, vol. 303, 1 January 2005 (2005-01-01), pages 1 - 17, XP008103243, ISBN: 978-1-58829-276-6 * |
JAISWAL J K ET AL: "USE OF QUANTUM DOTS FOR LIVE CELL IMAGING", NATURE METHODS, NATURE PUBLISHING GROUP, GB, vol. 1, no. 1, 1 October 2004 (2004-10-01), pages 73 - 78, XP008062140, ISSN: 1548-7091 * |
JYOTI K JAISWAL AND SANFORD M SIMON: "Optical Monitoring of Single Cells Using Quantum Dots", METHODS IN MOLECULAR BIOLOGY, vol. 374, January 2007 (2007-01-01), pages 93 - 104, XP008103244 * |
LENCER W I ET AL: "The intracellular voyage of cholera toxin: going retro", TRENDS IN BIOCHEMICAL SCIENCES, ELSEVIER, HAYWARDS, GB, vol. 28, no. 12, 1 December 2003 (2003-12-01), pages 639 - 645, XP004476602, ISSN: 0968-0004 * |
MICHALET X ET AL: "QUANTUM DOTS FOR LIVE CELLS IN VIVO IMAGING AND DIAGNOSTICS", SCIENCE, AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE, US, WASHINGTON, DC, vol. 307, no. 5709, 28 January 2005 (2005-01-28), pages 538 - 544, XP001222940, ISSN: 0036-8075 * |
SUGIMOTO Y ET AL: "Accumulation of cholera toxin and GM1 ganglioside in the early endosome of Niemann-Pick C1-deficient cells.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 23 OCT 2001, vol. 98, no. 22, 23 October 2001 (2001-10-23), pages 12391 - 12396, XP002518311, ISSN: 0027-8424 * |
VAN TILBORG GERALDA A F ET AL: "Annexin A5-conjugated quantum dots with a paramagnetic lipidic coating for the multimodal detection of apoptotic cells.", BIOCONJUGATE CHEMISTRY 2006 JUL-AUG, vol. 17, no. 4, July 2006 (2006-07-01), pages 865 - 868, XP002518313, ISSN: 1043-1802 * |
Also Published As
Publication number | Publication date |
---|---|
WO2009035770A2 (en) | 2009-03-19 |
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