+

WO2009035554A3 - Superior structure stability and selectivity of hairpin nucleic acid probes with an l-dna stem - Google Patents

Superior structure stability and selectivity of hairpin nucleic acid probes with an l-dna stem Download PDF

Info

Publication number
WO2009035554A3
WO2009035554A3 PCT/US2008/010466 US2008010466W WO2009035554A3 WO 2009035554 A3 WO2009035554 A3 WO 2009035554A3 US 2008010466 W US2008010466 W US 2008010466W WO 2009035554 A3 WO2009035554 A3 WO 2009035554A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
dna
selectivity
acid probes
stem
Prior art date
Application number
PCT/US2008/010466
Other languages
French (fr)
Other versions
WO2009035554A2 (en
Inventor
Weihong Tan
Youngmi Kim
Chaoyong James Yang
Original Assignee
Univ Florida
Weihong Tan
Youngmi Kim
Chaoyong James Yang
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Florida, Weihong Tan, Youngmi Kim, Chaoyong James Yang filed Critical Univ Florida
Publication of WO2009035554A2 publication Critical patent/WO2009035554A2/en
Publication of WO2009035554A3 publication Critical patent/WO2009035554A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6832—Enhancement of hybridisation reaction

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Hairpin-shaped nucleic acid probes capable of forming stable hybrids with target nucleic acid sequences are provided. In particular, an oligonucleotide probe for hybridization analysis is provided, wherein the probe comprises a non-natural enantiomeric DNA (L-DNA) that forms the stem and natural DNA (D-DNA) that forms the loop in the hairpin. The invention thereby provides stable probes that experience fewer stem invasions and demonstrate sensitive and selective hybridization to target nucleic acid sequences. The subject probes are useful for a variety of biological and biotechnological applications.
PCT/US2008/010466 2007-09-07 2008-09-08 Superior structure stability and selectivity of hairpin nucleic acid probes with an l-dna stem WO2009035554A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US96789407P 2007-09-07 2007-09-07
US60/967,894 2007-09-07

Publications (2)

Publication Number Publication Date
WO2009035554A2 WO2009035554A2 (en) 2009-03-19
WO2009035554A3 true WO2009035554A3 (en) 2009-05-14

Family

ID=40452748

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2008/010466 WO2009035554A2 (en) 2007-09-07 2008-09-08 Superior structure stability and selectivity of hairpin nucleic acid probes with an l-dna stem

Country Status (1)

Country Link
WO (1) WO2009035554A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201021873D0 (en) * 2010-12-23 2011-02-02 Mologen Ag DNA expression construct
GB201021867D0 (en) * 2010-12-23 2011-02-02 Mologen Ag Non-coding immunomodulatory DNA construct
LU92821B1 (en) 2015-09-09 2017-03-20 Mologen Ag Combination comprising immunostimulatory oligonucleotides
GB2542425A (en) 2015-09-21 2017-03-22 Mologen Ag Means for the treatment of HIV
CN116377136B (en) * 2023-03-14 2023-09-15 广东海洋大学 A fluorescent biosensor based on AIE material to rapidly detect the new coronavirus Omicron strain and its application

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020142309A1 (en) * 2000-07-14 2002-10-03 Nanibhushan Dattagupta Nucleic acid hairpin probes and uses thereof

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020142309A1 (en) * 2000-07-14 2002-10-03 Nanibhushan Dattagupta Nucleic acid hairpin probes and uses thereof

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
BRENT C. SATTERFIELD ET AL.: "Tentacle probes: eliminating false positives without sacrificing sensitivity.", NUCLEIC ACIDS RESEARCH, vol. 35, no. 10, 21 May 2007 (2007-05-21), pages E77 *
CAROLINE CREY-DESBIOLLES ET AL.: "Molecular beacons with a homo-DNA stem: improving target selectivity", NUCLEIC ACIDS RESEARCH, vol. 33, no. 8, 5 May 2005 (2005-05-05), pages E77 *
YOUNGMI KIM ET AL.: "Superior structure stability and selectivity of hairpin nucleic acid probes with an L-DNA stem.", NUCLEIC ACIDS RESEARCH, vol. 35, no. 21, 24 October 2007 (2007-10-24), pages 7279 - 7287 *

Also Published As

Publication number Publication date
WO2009035554A2 (en) 2009-03-19

Similar Documents

Publication Publication Date Title
WO2009152928A3 (en) Method for the quantitative analysis of nucleic acids, markers therefor and their use
WO2010117420A3 (en) Fret-labeled compounds and uses therefor
WO2002006531A3 (en) Nucleic acid hairpin probes and uses thereof
WO2009148560A8 (en) Methods and compositions for nucleic acid sequencing
GEP20125384B (en) Representations of nucleotide sequences
MX2018013367A (en) Methods for tagging dna-encoded libraries.
WO2007035684A3 (en) Method for quantitative detection of short rna molecules
WO2008054834A3 (en) Binary deoxyribozyme probes for nucleic acid analysis
WO2009012246A3 (en) Nicking and extension amplification reaction for the exponential amplification of nucleic acids
WO2010086622A8 (en) Adaptors for nucleic acid constructs in transmembrane sequencing
WO2009060124A3 (en) Use of oligonucleotides with modified bases in hybridization of nucleic acids
WO2004039953A3 (en) Array oligomer synthesis and use.
WO2013184754A3 (en) Spatial sequencing of nucleic acids using dna origami probes
WO2009073629A3 (en) Efficient shotgun sequencing methods
EP2089517A4 (en) Polymerase enzymes and reagents for enhanced nucleic acid sequencing
WO2010026488A3 (en) Methods and kits for nucleic acid sequencing
WO2009152353A3 (en) Reversible nucleosides and nucleotides terminators and their use in dna sequencing
WO2009035554A3 (en) Superior structure stability and selectivity of hairpin nucleic acid probes with an l-dna stem
NZ595626A (en) Wood and cell wall gene microarray
WO2014030066A3 (en) Rolling circle based methods identifying nucleic acids
WO2012092569A3 (en) Compositions comprising immunostimulatory nucleic acids and related methods
JP2013501508A5 (en)
WO2011140187A3 (en) Detecting chromosomal rearrangement
WO2009126678A3 (en) Detection of target nucleic acid sequences using fluorescence resonance energy transfer
McKenney et al. The evolution of substrate specificity by tRNA modification enzymes

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 08830340

Country of ref document: EP

Kind code of ref document: A2

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 08830340

Country of ref document: EP

Kind code of ref document: A2

点击 这是indexloc提供的php浏览器服务，不要输入任何密码和下载