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WO2009034927A1 - 細胞培養器具及びこれを用いた細胞培養方法 - Google Patents

細胞培養器具及びこれを用いた細胞培養方法 Download PDF

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Publication number
WO2009034927A1
WO2009034927A1 PCT/JP2008/066084 JP2008066084W WO2009034927A1 WO 2009034927 A1 WO2009034927 A1 WO 2009034927A1 JP 2008066084 W JP2008066084 W JP 2008066084W WO 2009034927 A1 WO2009034927 A1 WO 2009034927A1
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Prior art keywords
cell culture
wells
same
instrument
culture instrument
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PCT/JP2008/066084
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English (en)
French (fr)
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Kohji Nakazawa
Yusuke Sakai
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Kitakyushu Foundation For The Advancement Of Industry, Science And Technology
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Application filed by Kitakyushu Foundation For The Advancement Of Industry, Science And Technology filed Critical Kitakyushu Foundation For The Advancement Of Industry, Science And Technology
Priority to US12/733,563 priority Critical patent/US9121003B2/en
Priority to CN200880106786.8A priority patent/CN101815782B/zh
Priority to JP2009532166A priority patent/JP4576539B2/ja
Priority to EP08830570A priority patent/EP2189519A4/en
Publication of WO2009034927A1 publication Critical patent/WO2009034927A1/ja

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/12—Well or multiwell plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/067—Hepatocytes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0668—Trapping microscopic beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0636—Integrated biosensor, microarrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0829—Multi-well plates; Microtitration plates

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Developmental Biology & Embryology (AREA)
  • Clinical Laboratory Science (AREA)
  • Cell Biology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Reproductive Health (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Sustainable Development (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

　細胞を保持可能な複数のウェルを備えながら操作性に優れた細胞培養器具を提供する。 　本発明に係る細胞培養器具（１）は、細胞を保持可能な複数の第一のウェル（２１）を含むウェル群（２２）が形成された基部（１０）と、前記基部（１０）の前記ウェル群（２０）の周囲に立設されて、溶液を保持可能な第二のウェル（５０）を形成する枠部３と、を備える。  
PCT/JP2008/066084 2007-09-12 2008-09-05 細胞培養器具及びこれを用いた細胞培養方法 WO2009034927A1 (ja)

Priority Applications (4)

Application Number Priority Date Filing Date Title
US12/733,563 US9121003B2 (en) 2007-09-12 2008-09-05 Cell culture instrument and cell culture method using the same
CN200880106786.8A CN101815782B (zh) 2007-09-12 2008-09-05 细胞培养器具和使用该器具的细胞培养方法
JP2009532166A JP4576539B2 (ja) 2007-09-12 2008-09-05 細胞培養器具及びこれを用いた細胞培養方法
EP08830570A EP2189519A4 (en) 2007-09-12 2008-09-05 CELL CULTURE INSTRUMENT AND CELL CUKLTURE PROCESS WITH THIS

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007237159 2007-09-12
JP2007-237159 2007-09-12

Publications (1)

Publication Number Publication Date
WO2009034927A1 true WO2009034927A1 (ja) 2009-03-19

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PCT/JP2008/066084 WO2009034927A1 (ja) 2007-09-12 2008-09-05 細胞培養器具及びこれを用いた細胞培養方法

Country Status (5)

Country Link
US (1) US9121003B2 (ja)
EP (1) EP2189519A4 (ja)
JP (1) JP4576539B2 (ja)
CN (1) CN101815782B (ja)
WO (1) WO2009034927A1 (ja)

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JP2010233456A (ja) * 2009-03-30 2010-10-21 Kitakyushu Foundation For The Advancement Of Industry Science & Technology 細胞集合体形成器具、細胞集合体培養器具、細胞集合体転写キット及び細胞集合体の培養方法
JP2011004612A (ja) * 2009-06-23 2011-01-13 Hitachi Ltd 培養基材、培養シート、及び細胞培養方法
JP2011019413A (ja) * 2009-07-14 2011-02-03 Scivax Kk 細胞剥離抑制器具及びこれを用いた培養容器、細胞剥離抑制方法
JP2011234646A (ja) * 2010-05-07 2011-11-24 Hitachi Ltd 培養器材、及び培養シート
US20120100612A1 (en) * 2009-06-23 2012-04-26 Hitachi, Ltd. Culture substrate, culture sheet, and cell culture method
WO2012081470A1 (ja) * 2010-12-13 2012-06-21 株式会社資生堂 細胞凝集塊の形成方法
JPWO2011052281A1 (ja) * 2009-10-30 2013-03-14 国立大学法人 東京大学 神経スフェロイドネットワークの構築方法
JP2013524787A (ja) * 2010-04-06 2013-06-20 ユニバーシティ・オブ・カンザス 糖尿病治療のためのテンプレート島細胞および小型島細胞クラスター
US20130323839A1 (en) * 2010-12-22 2013-12-05 Hitachi, Ltd. Culture Substrate and Culture Sheet
WO2014042162A1 (ja) * 2012-09-14 2014-03-20 住友ベークライト株式会社 マイクロウェルプレート
WO2014119528A1 (ja) * 2013-01-29 2014-08-07 日本ゼオン株式会社 顕微鏡観察用容器の製造方法
WO2014156455A1 (ja) * 2013-03-28 2014-10-02 富士フイルム株式会社 細胞培養用具
WO2015087370A1 (ja) * 2013-12-12 2015-06-18 ヤマハ発動機株式会社 プレート構造体および該プレート構造体を含む対象物選別装置
JP2015136318A (ja) * 2014-01-22 2015-07-30 国立大学法人 筑波大学 細胞培養用デバイス
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JP2017506896A (ja) * 2014-02-18 2017-03-16 ドラッグアレイ， インコーポレイテッド マルチウェル分離装置および試薬送達デバイス
JP2017153373A (ja) * 2016-02-29 2017-09-07 株式会社ワークス 細胞検査部材
WO2017199737A1 (ja) * 2016-05-16 2017-11-23 富士フイルム株式会社 培養細胞の回収方法および培養細胞分散液
WO2020013207A1 (ja) * 2018-07-10 2020-01-16 株式会社日本触媒 細胞培養用シート
JP2020094044A (ja) * 2018-12-12 2020-06-18 株式会社日本触媒 ヒアルロン酸含有スフェロイド及びその作製方法
WO2020149246A1 (ja) * 2019-01-18 2020-07-23 東洋製罐グループホールディングス株式会社 培養容器、培養方法、及び培養装置
JP2020202778A (ja) * 2019-06-17 2020-12-24 国立大学法人 東京大学 培養方法
JPWO2019151114A1 (ja) * 2018-02-01 2021-01-28 Ａｇｃ株式会社 細胞培養容器
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JP2010233456A (ja) * 2009-03-30 2010-10-21 Kitakyushu Foundation For The Advancement Of Industry Science & Technology 細胞集合体形成器具、細胞集合体培養器具、細胞集合体転写キット及び細胞集合体の培養方法
JP2011004612A (ja) * 2009-06-23 2011-01-13 Hitachi Ltd 培養基材、培養シート、及び細胞培養方法
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JPWO2014156455A1 (ja) * 2013-03-28 2017-02-16 富士フイルム株式会社 細胞培養用具
JPWO2015087370A1 (ja) * 2013-12-12 2017-03-16 ヤマハ発動機株式会社 プレート構造体および該プレート構造体を含む対象物選別装置
WO2015087370A1 (ja) * 2013-12-12 2015-06-18 ヤマハ発動機株式会社 プレート構造体および該プレート構造体を含む対象物選別装置
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JPWO2019151114A1 (ja) * 2018-02-01 2021-01-28 Ａｇｃ株式会社 細胞培養容器
CN112384604A (zh) * 2018-07-10 2021-02-19 株式会社日本触媒 细胞培养用片
JPWO2020013207A1 (ja) * 2018-07-10 2021-06-24 株式会社日本触媒 細胞培養用シート
JP7105887B2 (ja) 2018-07-10 2022-07-25 株式会社日本触媒 細胞培養用シート
WO2020013207A1 (ja) * 2018-07-10 2020-01-16 株式会社日本触媒 細胞培養用シート
JP2020094044A (ja) * 2018-12-12 2020-06-18 株式会社日本触媒 ヒアルロン酸含有スフェロイド及びその作製方法
JP2020114191A (ja) * 2019-01-18 2020-07-30 東洋製罐グループホールディングス株式会社 培養容器、培養方法、及び培養装置
WO2020149246A1 (ja) * 2019-01-18 2020-07-23 東洋製罐グループホールディングス株式会社 培養容器、培養方法、及び培養装置
JP7348586B2 (ja) 2019-01-18 2023-09-21 東洋製罐グループホールディングス株式会社 培養容器、培養方法、及び培養装置
JP2020202778A (ja) * 2019-06-17 2020-12-24 国立大学法人 東京大学 培養方法
JP7344016B2 (ja) 2019-06-17 2023-09-13 国立大学法人 東京大学 培養方法
JP7648748B2 (ja) 2021-05-07 2025-03-18 エル・ピー・ケー・エフ・レーザー・アンド・エレクトロニクス・ソシエタス・ヨーロピア 細胞を培養する装置及び方法

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CN101815782A (zh) 2010-08-25
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EP2189519A1 (en) 2010-05-26
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