+

WO2009034842A1 - 核酸検出方法、および核酸検出キット - Google Patents

核酸検出方法、および核酸検出キット Download PDF

Info

Publication number
WO2009034842A1
WO2009034842A1 PCT/JP2008/065307 JP2008065307W WO2009034842A1 WO 2009034842 A1 WO2009034842 A1 WO 2009034842A1 JP 2008065307 W JP2008065307 W JP 2008065307W WO 2009034842 A1 WO2009034842 A1 WO 2009034842A1
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
acid detection
substance
dna
dna amplification
Prior art date
Application number
PCT/JP2008/065307
Other languages
English (en)
French (fr)
Inventor
Jun Tomono
Original Assignee
Kaneka Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kaneka Corporation filed Critical Kaneka Corporation
Priority to US12/677,189 priority Critical patent/US9063130B2/en
Priority to JP2009532131A priority patent/JPWO2009034842A1/ja
Priority to EP08831240.0A priority patent/EP2208795B1/en
Publication of WO2009034842A1 publication Critical patent/WO2009034842A1/ja

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/566—Immunoassay; Biospecific binding assay; Materials therefor using specific carrier or receptor proteins as ligand binding reagents where possible specific carrier or receptor proteins are classified with their target compounds
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Zoology (AREA)
  • Cell Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

　第１物質が特異的に結合することが可能な第１物質結合部位を含む、核酸増幅法によるＤＮＡ増幅断片を用意する。該ＤＮＡ増幅断片を上記第１物質に結合させることによって、該ＤＮＡ増幅断片を濃縮する。ＤＮＡは、濃縮により高感度に検出することができる。したがって、上記構成によれば、核酸増幅法によるＤＮＡ増幅断片を、特殊な装置を必要とすることなく、簡便かつ高精度に検出することができる。
PCT/JP2008/065307 2007-09-11 2008-08-27 核酸検出方法、および核酸検出キット WO2009034842A1 (ja)

Priority Applications (3)

Application Number Priority Date Filing Date Title
US12/677,189 US9063130B2 (en) 2007-09-11 2008-08-27 Nucleic acid detection method and nucleic acid detection kit
JP2009532131A JPWO2009034842A1 (ja) 2007-09-11 2008-08-27 核酸検出方法、および核酸検出キット
EP08831240.0A EP2208795B1 (en) 2007-09-11 2008-08-27 Nucleic acid detection method, and nucleic acid detection kit

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007235935 2007-09-11
JP2007-235935 2007-09-11

Publications (1)

Publication Number Publication Date
WO2009034842A1 true WO2009034842A1 (ja) 2009-03-19

Family

ID=40451852

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/065307 WO2009034842A1 (ja) 2007-09-11 2008-08-27 核酸検出方法、および核酸検出キット

Country Status (4)

Country Link
US (1) US9063130B2 (ja)
EP (1) EP2208795B1 (ja)
JP (2) JPWO2009034842A1 (ja)
WO (1) WO2009034842A1 (ja)

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012070618A1 (ja) * 2010-11-24 2012-05-31 株式会社カネカ 増幅核酸検出方法及び検出デバイス
CN102934322A (zh) * 2010-06-09 2013-02-13 日产自动车株式会社 电池充电系统
WO2013039228A1 (ja) 2011-09-14 2013-03-21 日本碍子株式会社 標的核酸の検出方法
WO2014007289A1 (ja) 2012-07-05 2014-01-09 日本碍子株式会社 核酸クロマトグラフィー法、核酸クロマトグラフィー用組成物及びこれを含むキット
US9783844B2 (en) 2012-04-27 2017-10-10 Kaneka Corporation Method for amplifying nucleic acid and method for detecting amplified nucleic acid
US10392652B2 (en) 2013-11-22 2019-08-27 Kaneka Corporation Micro RNA detection method using two primers to produce an amplified double stranded DNA fragment having a single stranded region at one end
WO2024232313A1 (ja) * 2023-05-08 2024-11-14 国立研究開発法人産業技術総合研究所 検出装置と検出方法と加温装置

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103119178B (zh) * 2010-09-22 2017-05-17 株式会社钟化 核酸的检测方法以及装置、试剂盒
KR101904787B1 (ko) 2011-03-04 2018-10-08 가부시키가이샤 가네카 핵산의 검출 방법 및 그 방법에 사용하는 디바이스, 키트
DE102015115836A1 (de) 2015-09-18 2017-03-23 Biotype Diagnostic Gmbh Bestätigungstest für primäre Nukleinsäure-Amplifikate in einem kontinuierlichen Reaktionsansatz und unmittelbare Auswertung mittels immunchromatographischer Verfahren

Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6166964A (ja) 1984-09-10 1986-04-05 Konishiroku Photo Ind Co Ltd タンパク質又は核酸の高感度検出方法
JPS61274697A (ja) 1985-03-28 1986-12-04 エフ．ホフマン―ラ　ロシュ　アクチェンゲゼルシャフト 核酸配列の増幅及び検出方法
JPH029400A (ja) 1988-03-25 1990-01-12 Bioprobe Syst 非放射性標識によって検出される核酸を発色させる方法及び該方法を実施するための反応物
JPH09187275A (ja) 1995-11-15 1997-07-22 Becton Dickinson & Co 核酸増幅の蛍光偏光検出法
WO2001030993A1 (fr) * 1999-10-25 2001-05-03 Wakunaga Pharmaceutical Co., Ltd. Procede de detection d'un acide nucleique cible
WO2001083817A1 (fr) 2000-05-01 2001-11-08 Eiken Kagaku Kabushiki Kaisha Procede de detection de produit de reaction de synthese d'acide nucleique
JP2002186481A (ja) 2000-12-18 2002-07-02 Eiken Chem Co Ltd 光学的特性の測定による核酸増幅産物の検出方法
JP2003000299A (ja) 2001-06-18 2003-01-07 Fuji Photo Film Co Ltd ターゲット核酸断片の検出方法及びターゲット核酸断片の検出キット
WO2004042057A1 (ja) * 2002-11-07 2004-05-21 Daiichi Pure Chemicals Co., Ltd. 遺伝子変異検出法

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU629845B2 (en) * 1988-08-30 1992-10-15 Abbott Laboratories Detection and amplification of target nucleic acid sequences
AU633036B2 (en) * 1988-12-09 1993-01-21 Chemicon International, Inc. Amplified dna assay
US5482836A (en) * 1993-01-14 1996-01-09 The Regents Of The University Of California DNA purification by triplex-affinity capture and affinity capture electrophoresis
US6037127A (en) * 1994-03-31 2000-03-14 E. I. Du Pont De Nemours And Company Method for detection of non-denatured nucleic acid fragments
US5547861A (en) * 1994-04-18 1996-08-20 Becton, Dickinson And Company Detection of nucleic acid amplification
US6218105B1 (en) * 1996-07-19 2001-04-17 Kathleen S. Hall High throughput papilloma virus in vitro infectivity assay
WO2001066688A1 (en) * 2000-03-08 2001-09-13 Imagene Technology, Inc. Lateral flow pcr with amplicon concentration and detection
EP1290215A2 (en) * 2000-06-07 2003-03-12 Cyclacel Limited Methods for monitoring enzyme activity
GB0016833D0 (en) * 2000-07-07 2000-08-30 Lee Helen Improved dipstick assays (2)
US20030082559A1 (en) * 2001-01-19 2003-05-01 David Beach Methods and reagents for amplification and manipulation of vector and target nucleic acid sequences
GR1004393B (el) * 2002-11-07 2003-11-28 ΑΥΤΟΜΑΤΟΙ ΑΝΑΛΥΤΕΣ ΚΑΙ ΔΙΑΓΝΩΣΤΙΚΑ ΑΝΤΙΔΡΑΣΤΗΡΙΑ MEDICON HELLAS ΑΝΩΝΥΜΗ ΕΤΑΙΡΕΙΑ δ.τ. MEDICON HELLAS S.A. Μεθοδος ανιχνευσης ειδικων αλληλουχιων νουκλεικων οξεων σε ταινια ξηρων αντιδραστηριων
ES2363501T3 (es) * 2003-05-07 2011-08-05 Coris Bioconcept Sprl Dispositivo oligocromático de un paso y procedimiento de uso.
ES2384571T3 (es) 2004-03-05 2012-07-09 Sirs-Lab Gmbh Procedimiento para el enriquecimiento y/o la separación de ADN procariota mediante una proteína que se une específicamente a ADN que contiene motivos CpG no metilados
US20050227275A1 (en) * 2004-04-07 2005-10-13 Access Bio, Inc. Nucleic acid detection system
WO2007116298A2 (en) * 2006-04-10 2007-10-18 3M Innovative Properties Company Nucleic acid detection using lateral flow methods
US20080127907A1 (en) * 2006-12-04 2008-06-05 James Purtell Animal cast protection system
JP5211790B2 (ja) * 2007-03-26 2013-06-12 住友化学株式会社 Ｄｎａメチル化測定方法
US20090142757A1 (en) * 2007-11-30 2009-06-04 Asiagen Corporation Strip and method for detecting nucleotide amplification products of mycobacterium tuberculosis and non-tuberculous mycobacterium

Patent Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6166964A (ja) 1984-09-10 1986-04-05 Konishiroku Photo Ind Co Ltd タンパク質又は核酸の高感度検出方法
JPS61274697A (ja) 1985-03-28 1986-12-04 エフ．ホフマン―ラ　ロシュ　アクチェンゲゼルシャフト 核酸配列の増幅及び検出方法
JPS62281A (ja) 1985-03-28 1987-01-06 エフ．ホフマン―ラ　ロシュ　アクチェンゲゼルシャフト 核酸配列の増幅方法
JPH029400A (ja) 1988-03-25 1990-01-12 Bioprobe Syst 非放射性標識によって検出される核酸を発色させる方法及び該方法を実施するための反応物
JPH09187275A (ja) 1995-11-15 1997-07-22 Becton Dickinson & Co 核酸増幅の蛍光偏光検出法
WO2001030993A1 (fr) * 1999-10-25 2001-05-03 Wakunaga Pharmaceutical Co., Ltd. Procede de detection d'un acide nucleique cible
WO2001083817A1 (fr) 2000-05-01 2001-11-08 Eiken Kagaku Kabushiki Kaisha Procede de detection de produit de reaction de synthese d'acide nucleique
JP2002186481A (ja) 2000-12-18 2002-07-02 Eiken Chem Co Ltd 光学的特性の測定による核酸増幅産物の検出方法
JP2003000299A (ja) 2001-06-18 2003-01-07 Fuji Photo Film Co Ltd ターゲット核酸断片の検出方法及びターゲット核酸断片の検出キット
WO2004042057A1 (ja) * 2002-11-07 2004-05-21 Daiichi Pure Chemicals Co., Ltd. 遺伝子変異検出法

Non-Patent Citations (9)

* Cited by examiner, † Cited by third party
Title
BALANT ET AL., EUR. J. CLIN. PHARMACOL., vol. 36, 1989, pages 551 - 554
GADGIL,HIMANSHU,ET AL.: "Affinity purification of DNA-binding proteins", JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS, vol. 49, no. 1-3, 2001, pages 607 - 624, XP002450171 *
GADGIL,HIMANSHU,ET AL.: "DNA affinity chromatography of transcription factors.", ANALYTICAL BIOCHEMISTRY, vol. 290, no. 2, 2001, pages 147 - 178, XP008133187 *
GRAM; BRSEN, EUROPEAN CONSENSUS CONFERENCE ON PHARMACOGENETICS. COMMISSION OF THE EUROPEAN COMMUNITIES, 1990, pages 87 - 96
HIRATSUKA MASAHIRO,ET AL.: "Genotyping of single nucleotide polymorphisms (SNPs) influencing drug response by competitive allele-specific short oligonucleotide hybridization (CASSOH) with immunochromatographic strip.", DRUG METABOLISM AND PHARMACOKINETICS, vol. 19, no. 4, 2004, pages 303 - 307, XP008133729 *
MASATOSHI MATSUBARA ET AL: ""Men`eki chromatography ni yoru Ebi no White Spot Virus no Kan`i Kenshutsu Hoho no Kaihatsu"", XP008133227, Retrieved from the Internet <URL:http://www.jst.go.jp/tt/dokusou/pdf/res_pdf/res_14/res_14_40.pdf#search=`men`eki%20kuromato%20shindan> [retrieved on 20081001] *
POTUZAK,HANS AND DEAN,PETER D.G.: "Affinity chromatography on columns containing nucleic acids.", FEBS LETTERS, vol. 88, no. 2, 1978, pages 161 - 166, XP025600777 *
See also references of EP2208795A4
SHIRAISHI MASAHIKO,ET AL.: "Methyl-CpG binding domain column chromatography as a tool for the analysis of genomic DNA methylation.", ANALYTICAL BIOCHEMISTRY, vol. 329, no. 1, 2004, pages 1 - 10, XP004506459 *

Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102934322A (zh) * 2010-06-09 2013-02-13 日产自动车株式会社 电池充电系统
CN102934322B (zh) * 2010-06-09 2015-04-08 日产自动车株式会社 电池充电系统
WO2012070618A1 (ja) * 2010-11-24 2012-05-31 株式会社カネカ 増幅核酸検出方法及び検出デバイス
US9920356B2 (en) 2010-11-24 2018-03-20 Kaneka Corporation Amplified nucleic acid detection method and detection device
US10829805B2 (en) 2010-11-24 2020-11-10 Kaneka Corporation Amplified nucleic acid detection method and detection device
WO2013039228A1 (ja) 2011-09-14 2013-03-21 日本碍子株式会社 標的核酸の検出方法
US9783844B2 (en) 2012-04-27 2017-10-10 Kaneka Corporation Method for amplifying nucleic acid and method for detecting amplified nucleic acid
WO2014007289A1 (ja) 2012-07-05 2014-01-09 日本碍子株式会社 核酸クロマトグラフィー法、核酸クロマトグラフィー用組成物及びこれを含むキット
US10392652B2 (en) 2013-11-22 2019-08-27 Kaneka Corporation Micro RNA detection method using two primers to produce an amplified double stranded DNA fragment having a single stranded region at one end
WO2024232313A1 (ja) * 2023-05-08 2024-11-14 国立研究開発法人産業技術総合研究所 検出装置と検出方法と加温装置

Also Published As

Publication number Publication date
JPWO2009034842A1 (ja) 2010-12-24
EP2208795B1 (en) 2016-03-30
EP2208795A1 (en) 2010-07-21
JP5727570B2 (ja) 2015-06-03
JP2014000098A (ja) 2014-01-09
US9063130B2 (en) 2015-06-23
US20100330564A1 (en) 2010-12-30
EP2208795A4 (en) 2011-02-02

Similar Documents

Publication Publication Date Title
WO2009034842A1 (ja) 核酸検出方法、および核酸検出キット
WO2013050881A3 (en) Direct nucleic acid analysis
WO2005021731A3 (en) Soluble analyte detection and amplification
ATE479780T1 (de) Qpcr unter verwendung eines ionen-sensitiven feldeffekt-transistors zur ph-messung
WO2013130512A3 (en) Methods and uses for molecular tags
WO2012097081A3 (en) Protein detection method
ATE509274T1 (de) Biosensorvorrichtung und verfahren zu ihrer verwendung
CL2011002228A1 (es) Aparato para el analisis integrado de acidos nucleicos en tiempo real, y metodo de detecicion de dichas moleculas.
ATE544063T1 (de) Nanoporenvorrichtung und verfahren zur nukleinsäureanalyse
WO2009060847A1 (ja) 核酸増幅用サンプルの調製方法及び調製キット
WO2009129505A3 (en) Compositions, methods, and kits using synthetic probes for determining the presence of a target nucleic acid
WO2010019414A3 (en) Detecting nucleic acid
EA201170933A1 (ru) Анализ нуклеиновых кислот, полученных из единичных клеток
WO2013157917A3 (en) A biosensor and preparation method thereof
IN2014KN02647A (ja)
WO2010065781A3 (en) Lateral flow strip assay with immobilized conjugate
EP2314680A4 (en) METHOD FOR THE AMPLIFICATION OF A TARGET NUCLEIC ACID SEQUENCE, METHOD FOR METHODIC DETECTION USING THE METHOD AND REAGENT FOR USE IN THE PROCEDURES
WO2010141075A8 (en) Specific analysis of ketone and aldehyde analytes using reagent compounds, labeling strategies, and mass spectrometry workflow
EP2254897A4 (en) METHOD, REAGENTS AND KITS FOR DETECTING NUCLEIC ACID MOLECULES
EP2772761A3 (en) Nucleic acid element for use in analysis, and analytical method, analytical reagent and analytical instrument using same
WO2009074983A3 (en) Assay for detecting circulating free nucleic acids
WO2009057829A3 (en) Nucleic acid primer set for detection of drug-resistant strain of hepatitis b virus, assay kit, and method of detecting drug-resistant strain of hepatitis b virus
WO2011127001A3 (en) Quantum dot-based optical sensors for rapid detection and quantitative analysis of biomolecules and biological materials
EP2504348A4 (en) METHODS, REAGENTS AND KITS FOR DETECTING NUCLEIC ACID MOLECULES
WO2012064978A3 (en) Compositions, methods, and kits for detecting and identifying mycobacteria

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 08831240

Country of ref document: EP

Kind code of ref document: A1

ENP Entry into the national phase

Ref document number: 2009532131

Country of ref document: JP

Kind code of ref document: A

WWE Wipo information: entry into national phase

Ref document number: 12677189

Country of ref document: US

NENP Non-entry into the national phase

Ref country code: DE

WWE Wipo information: entry into national phase

Ref document number: 2008831240

Country of ref document: EP

点击 这是indexloc提供的php浏览器服务，不要输入任何密码和下载