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WO2009002719A1 - Acide nucléique inhibiteur des liposomes contre les protéines stat - Google Patents

Acide nucléique inhibiteur des liposomes contre les protéines stat Download PDF

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Publication number
WO2009002719A1
WO2009002719A1 PCT/US2008/066753 US2008066753W WO2009002719A1 WO 2009002719 A1 WO2009002719 A1 WO 2009002719A1 US 2008066753 W US2008066753 W US 2008066753W WO 2009002719 A1 WO2009002719 A1 WO 2009002719A1
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Prior art keywords
cancer
composition
nucleic acid
sirna
stat3
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PCT/US2008/066753
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English (en)
Inventor
Charles N. Landen
Aparna A. Kamat
Anil K. Sood
Gabriel Lopez-Berestein
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The Board Of Regents Of The University Of Texas System
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Publication of WO2009002719A1 publication Critical patent/WO2009002719A1/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering nucleic acids [NA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2320/00—Applications; Uses
    • C12N2320/30—Special therapeutic applications
    • C12N2320/32—Special delivery means, e.g. tissue-specific

Definitions

  • the present invention relates generally to the fields of molecular biology, RNA interference, and oncology. More particularly, the invention concerns compositions comprising an inhibitory nucleic acid, wherein the inhibitory nucleic acid is targeted to a nucleic acid encoding a STAT protein.
  • the invention also generally pertains to methods of treating a disease associated with overexpression of a STAT protein in a subject, involving administering to the subject a pharmaceutically effective amount of a composition comprising an inhibitory nucleic acid and a lipid component, wherein the inhibitory nucleic acid is targeted to a nucleic acid encoding a STAT.
  • norepinephrine While circulating plasma levels of norepinephrine are only about 10-1000 pM in a normal individual, and may reach as high as 100 nM in conditions of stress (Schmidt and Kraft, 1996), catecholamine levels in the ovary are at least 100 times higher. Studies suggest that within the parenchyma of the ovary, and thus the tumor microenvironment, concentrations may reach as high as 10 ⁇ M (Lara et al, 2001; Lara et al, 2002).
  • STAT Signal Transducers and Activator of Transcription
  • the mammalian STAT family members include STATl, STAT2, STAT3, STAT4, STAT5 a and b, and STAT6.
  • STAT3 is involved in numerous processes that relate to cancer progression (CaIo et al. , 2003). Constitutive STAT3 activation has been noted in numerous cancer types, such as hematologic, head and neck, brain, breast, lung, prostate, pancreas, and ovarian cancers (Bromberg, 2002; Garcia et al, 2001; Song and Grandis, 2000; Lin et al, 2000; Huang et al, 2000; Sartor et al, 1997; Garcia et al, 1997). STAT3 has transforming properties in and of itself, and is a participant in many other processes associated with oncogenesis (Bowman et al, 2000).
  • STAT3 has been shown to be a transcriptional regulator or MMP-2 in human melanoma cells (Xie et al, 2004; Kortylewski et al, 2005; Xie et al, 2006).
  • STAT3 is required for maximal induction of MMP-I by EGFR in bladder cancer cells (Itoh et al, 2006) and by fibroblast growth factor- 1 in prostate cancer cells (Udayakumar et al, 2004).
  • STAT3 activation is strongly correlated with MMP expression in breast cancer (Hsieh et al. , 2005), and the relationship is implicated in carcinogenesis (Dechow et al., 2004).
  • Liposomes have been used previously for drug delivery ⁇ e.g., delivery of a chemotherapeutic). Cationic liposomes are described in PCT publications WO02/100435A1, WO03/015757A1, WO04029213A2, and U.S. Patents 5,962,016, 5,030,453, and 6,680,068, and U.S. Patent Application 2004/0208921, all of which are hereby incorporated by reference in their entirety without disclaimer. A process of making liposomes is also described in WO04/002453A1. Neutral lipids have also been incorporated into cationic liposomes (see, e.g., Farhood et al, 1995).
  • Cationic liposomes have been used to deliver siRNA to various cell types (see, e.g., Sioud and Sorensen, 2003; U.S. Patent Application 2004/0204377; Duxbury et al, 2004; Donze and Picard, 2002).
  • Neutral liposomes have been tested to a limited degree. Neutral liposomes were used to deliver therapeutic antisense oligonucleotides in U.S. Patent Application 2003/0012812 and siRNA in WO 2006/113679.
  • the present invention is based on the finding of an association between the presence of neuroendocrine hormones associated with stress and elevation of STAT protein expression in disease, such as cancer.
  • the inventors have found that neuroendocrine hormones that are associated with stress have the potential to activate STAT3 and induce its nuclear translocation and DNA binding.
  • the invention is also based on the finding that the activation of STAT proteins in cancer can be ameliorated by downregulation using inhibitory nucleic acids.
  • compositions that includes: (1) an inhibitory nucleic acid ⁇ e.g., siNA), wherein the nucleic acid inhibits the expression of a gene encoding a STAT or encodes a nucleic acid that inhibits the expression of a gene encoding a STAT; and (2) a lipid component that includes one or more phospholipids, wherein the lipid component has an essentially neutral charge.
  • an inhibitory nucleic acid e.g., siNA
  • the inhibitory nucleic acid may inhibit the expression of any STAT protein.
  • the STAT protein may be STATl, STAT2, STAT3, STAT4, STAT5a, STAT5b, or STAT6.
  • the STAT is STAT3.
  • Inhibitory nucleic acids or "siNA”, as used herein, is defined as a short interfering nucleic acid.
  • An inhibitory nucleic acid includes a siRNA, a nucleic acid encoding a siRNA, or shRNA (short hairpin RNA), a ribozyme, or an antisense nucleic acid molecule that specifically hybridize to a nucleic acid molecule encoding a target protein or regulating the expression of the target protein.
  • “Specific hybridization” means that the siRNA, shRNA, ribozyme or antisense nucleic acid molecule hybridizes to the targeted nucleic acid molecule and regulates its expression.
  • specific hybridization also means that no other genes or transcripts are affected.
  • siNA examples include but are not limited to RNAi, double-stranded RNA, and siRNA.
  • a siNA can inhibit the transcription or translation of a gene in a cell.
  • a siNA may be from 16 to 1000 or more nucleotides long, and in certain embodiments from 18 to 100 nucleotides long.
  • the siNA may be 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 100, 200, 300, 500, or more nucleotides long.
  • the siNA may comprise a nucleic acid and/or a nucleic acid analog.
  • a siNA will inhibit the translation of a single gene within a cell; however, in certain embodiments, a siNA will inhibit the translation of more than one gene within a cell.
  • the double stranded nucleic acid can comprise 18 to 30, 19 to 25, 20 to 23, or 21 contiguous nucleobases or nucleobase pairs.
  • the siNA component comprises a single species of siRNA or more than one species of siRNA. In other embodiments, the siNA component comprises a 2, 3, 4 or more species of siRNA that target 1, 2, 3, 4, or more genes. In some embodiments, the siNA is encapsulated in the lipid component.
  • the siNA component may inhibit the expression of a STAT protein, or a truncated form on a STAT protein.
  • STAT3-beta is a truncated form of STAT3 that contains the dimerization and DNA binding domain but lacks the transactivation domain.
  • siRNA can be obtained from commercial sources, natural sources, or can be synthesized using any of a number of techniques well-known to those of ordinary skill in the art. For example, one commercial source of predesigned siRNA is Ambion®, Austin, TX. Another is Qiagen® (Valencia, CA).
  • An inhibitory nucleic acid that can be applied in the compositions and methods of the present invention may be any nucleic acid sequence that has been found by any source to be a validated downregulator of a STAT protein.
  • the inhibitory nucleic acid is Qiagen® (Valencia, CA) validated siRNA product Catalog Number SIO2662338.
  • the lipid component may be in the form of a liposome.
  • the siNA e.g., a siRNA
  • Encapsulate refers to the lipid or liposome forming an impediment to free difussion into solution by an association with or around an agent of interest, e.g., a liposome may encapsulate an agent within a lipid layer or within an aqeous compartement inside or between lipid layers.
  • the composition is comprised in a pharmaceutically acceptable carrier.
  • the pharmaceutically acceptable carrier may be formulated for administration to a human subject or patient.
  • the lipid component has an essentially neutral charge because it comprises a neutral phospholipid or a net neutral charge.
  • a neutral phospholipid may be a phosphatidylcholine, such as DOPC, egg phosphatidylcholine (“EPC”), dilauryloylphosphatidylcholine (“DLPC”), dimyristoylphosphatidylcholine (“DMPC”), dipalmitoylphosphatidylcholine (“DPPC”), distearoylphosphatidylcholine (“DSPC”), l-myristoyl-2-palmitoyl phosphatidylcholine (“MPPC”), l-palmitoyl-2-myristoyl phosphatidylcholine (“PMPC”), l-palmitoyl-2-stearoyl phosphatidylcholine (“PSPC”), 1- stearoyl-2-palmitoyl phosphatidylcholine (“SPPC”),
  • SPPC 1- stearoy
  • the neutral phospholipid can be a phosphatidylethanolamine, such as dioleoylphosphatidylethanolamine (“DOPE”), distearoylphophatidylethanolamine (“DSPE”), dimyristoyl phosphatidylethanolamine (“DMPE”), dipalmitoyl phosphatidylethanolamine (“DPPE”), palmitoyloeoyl phosphatidylethanolamine (“POPE”), or lysophosphatidylethanolamine.
  • DOPE dioleoylphosphatidylethanolamine
  • DSPE distearoylphophatidylethanolamine
  • DMPE dimyristoyl phosphatidylethanolamine
  • DPPE dipalmitoyl phosphatidylethanolamine
  • POPE palmitoyloeoyl phosphatidylethanolamine
  • lysophosphatidylethanolamine lysophosphatidylethanolamine.
  • a lipid component can have an essentially neutral charge because it comprises a positively charged lipid and a negatively charged lipid.
  • the lipid component may further comprise a neutrally charged lipid(s) or phospholipid(s).
  • the positively charged lipid may be a positively charged phospholipid.
  • the negatively charged lipid may be a negatively charged phospholipid.
  • the negatively charged phospholipid may be a phosphatidylserine, such as dimyristoyl phosphatidylserine ("DMPS"), dipalmitoyl phosphatidylserine (“DPPS”), or brain phosphatidylserine ("BPS").
  • DMPS dimyristoyl phosphatidylserine
  • DPPS dipalmitoyl phosphatidylserine
  • BPS brain phosphatidylserine
  • the negatively charged phospholipid may be a phosphatidylglycerol, such as dilauryloylphosphatidylglycerol (“DLPG”), dimyristoylphosphatidylglycerol (“DMPG”), dipalmitoylphosphatidylglycerol (“DPPG”), distearoylphosphatidylglycerol (“DSPG”), or dioleoylphosphatidylglycerol (“DOPG”).
  • the composition further comprises cholesterol or polyethyleneglycol (PEG).
  • a phospholipid is a naturally-occurring phospholipid.
  • a phospholipid is a synthetic phospholipid.
  • composition may further comprise a chemotherapeutic or other anti-cancer agent, which may or may not be encasulated in a lipid component or liposome of the invention.
  • chemotherapeutic agent is a taxane or taxane derivative.
  • Such agents include docetaxel, paclitaxel, abraxane, 7-epipaclitaxel, t-acetyl paclitaxel, 10-desacetyl -paclitaxel, 10-desacetyl-7-epipaclitaxel, 7-xylosylpaclitaxel, lO-desacetyl-7-glutarylpaclitaxel, 7-N,N-dimethylglycylpaclitaxel, 7-L-alanylpaclitaxel, or a mixture thereof.
  • the chemotherapeutic agent is cisplatin (CDDP), carboplatin, procarbazine, mechlorethamine, cyclophosphamide, camptothecin, ifosfamide, melphalan, chlorambucil, busulfan, nitrosurea, dactinomycin, daunorubicin, doxorubicin, bleomycin, plicomycin, mitomycin, etoposide (VP 16), tamoxifen, raloxifene, estrogen receptor binding agents, taxol, gemcitabien, navelbine, farnesyl-protein tansferase inhibitors, transplatinum, 5-fluorouracil, vincristine, vinblastin, methotrexate, or combinations thereof.
  • CDDP cisplatin
  • carboplatin carboplatin
  • procarbazine mechlorethamine
  • cyclophosphamide camptothecin
  • the chemotherapeutic agent is docetaxel.
  • the present invention also generally pertains to methods of treating a subject with a hyperproliferative disease involving administering to the subject a pharmaceutically effective amount of a composition comprising an inhibitory nucleic acid, wherein the nucleic acid inhibits the expression of a gene encoding a STAT or encodes a nucleic acid that inhibits the expression of a gene encoding a STAT; and (2) a lipid component.
  • the composition for example, can be any of the compositions discussed above.
  • the inhibitory nucleic acid may inhibit the expression of any gene encoding a STAT protein.
  • the STAT may be STATl, STAT2, STAT3, STAT4, STAT5a, STAT5b, or STAT6. In particular embodiments, the STAT is STAT3.
  • the cancer can be any type of cancer, such as breast cancer, lung cancer, prostate cancer, ovarian cancer, brain cancer, liver cancer, cervical cancer, colon cancer, renal cancer, skin cancer, head and neck cancer, bone cancer, esophageal cancer, bladder cancer, uterine cancer, lymphatic cancer, stomach cancer, pancreatic cancer, testicular cancer, lymphoma, or leukemia.
  • the cancer is ovarian cancer and the STAT is STAT3.
  • the method further includes identifying a subject in need of treatment. Such identification can be by any method known to those of ordinary skill in the art, such as based on clinical examination, based on identification of a particular stage or grade of tumor, and so forth.
  • the composition is administered in the form of a vaccine.
  • the methods of the invention further comprise administering an additional anticancer therapy to the subject.
  • the additional therapy may comprise administering a chemotherapeutic, a surgery, a radiation therapy, an immunotherapy, and/or a gene therapy.
  • the chemotherapy is docetaxel, paclitaxel, cisplatin (CDDP), carboplatin, procarbazine, mechlorethamine, cyclophosphamide, camptothecin, ifosfamide, melphalan, chlorambucil, busulfan, nitrosurea, dactinomycin, daunorubicin, doxorubicin, bleomycin, plicomycin, mitomycin, etoposide (VP 16), tamoxifen, raloxifene, estrogen receptor binding agents, taxol, gemcitabien, navelbine, farnesyl-protein tansferase inhibitors, transplatinum, 5-fluorouracil, vincristin, vinblastin, methotrexate, or combinations thereof.
  • CDDP docetaxel
  • carboplatin carboplatin
  • procarbazine mechlorethamine
  • cyclophosphamide camptothecin
  • the chemotherapy is a taxane such as docetaxal or paclitaxel.
  • the chemotherapy can be delivered before, during, after, or combinations thereof relative to a neutral lipid composition of the invention.
  • a chemotherapy can be delivered within 0, 1, 5, 10, 12, 20, 24, 30, 48, or 72 hours or more of the neutral lipid composition.
  • the neutral lipid composition, the second anti-cancer therapy, or both the neutral lipid composition and the anti-cancer therapy can be administered intratumorally, intravenously, intraperitoneally, orally or by various combinations thereof.
  • the therapeutic agents and compositions set forth herein can be administered to the patient using any technique known to those of ordinary skill in the art. For example, administration may be intravenously, intraperitoneally, intratracheally, intratumorally, intramuscularly, endoscopically, intralesionally, percutaneously, subcutaneously, regionally, or by direct injection or perfusion.
  • the invention concerns a method of treating a subject with ovarian cancer comprising administering to the subject a pharmaceutically effective amount of an siRNA, wherein the siRNA is targeted to a gene that encodes STAT3.
  • the method further involves administering a chemotherapeutic agent to the subject.
  • the chemotherapeutic agent may be administered prior to the siRNA, concurrently with the siRNA, or following administration of the siRNA.
  • the chemotherapeutic agent can be any of those agents discussed above and elsewhere in this specification.
  • the subject has a tumor and the method of treating cancer is further defined as a method to reduce the invasiveness of a tumor in the subject.
  • the tumor is ovarian cancer.
  • the present invention also generally pertains to methods of reducing stress hormone-inducted activation of STAT3 in a subject, involving administering to the subject a pharmaceutically effective amount of any of the compositions set forth above.
  • the stress hormone-induced activation is norepinephrine- or epinephrine- induced activation of STAT3.
  • the subject has a hyperproliferative disease, such as cancer.
  • the cancer is ovarian cancer.
  • the words “comprising” (and any form of comprising, such as “comprise” and “comprises”), “having” (and any form of having, such as “have” and “has”), "including” (and any form of including, such as “includes” and “include”), or “containing” (and any form of containing, such as “contains” and “contain”) are inclusive or open-ended and do not exclude additional, unrecited elements or method steps.
  • FIG. IA, IB, 1C, and ID Effect of norepinephrine and epinephrine on phospho-STAT3.
  • the ovarian cancer cell lines SKO V3 and EG were serum starved for 15 hours before addition of increasing concentrations of NE or Epi (FIG. IA). Cell lysate was collected 3 hours later, and subjected to Western blot using anti- phosphorylated STAT3 antibody.
  • pSTAT3 increases significantly with as low as 0.1 ⁇ M of both hormones.
  • FIG. 2A shows constitutive presence of cytoplasmic STAT3 without NE stimulation compared to a negative control (no anti-STAT3 antibody, FIG. 2B). With 1 ⁇ M and 10 ⁇ M NE exposure (FIG. 2C), the nuclear signal increases dramatically, an effect inhibited by pretreatment with propanolol (FIG. 2D).
  • FIG. 3 Norepinephrine induces STAT3 DNA binding.
  • FIG. 4A and 4B Blockade of protein kinase A, but not IL-6, prevents NE- induced STAT3 activation.
  • Pretreatment of SKOV3 cells with 1 ⁇ M of the protein kinase A-specif ⁇ c inhibitor KT5720 reduces the increase in pSTAT3 seen with NE, as measured by Western blot (FIG. 4A).
  • pretreatment with 50 ⁇ g/ml anti-IL-6 antibody a dose previously shown to inhibit the activating effect of IL-6 on STAT3, was not effective in reducing the NE-induced effects (FIG. 4B).
  • FIG. 5A, 5B, 5C, 5D STAT3-mediated increases in cellular invasion and MMP expression with norepinephrine.
  • SKOV3 or EG cells were exposed to 10 ⁇ M NE alone and with STAT3 -specific siRNA or control siRNA, and introduced into the MICS system to determine effects on invasion (FIG. 5B).
  • the percent of cells invading through a human-defined matrix increased by 2.6-3.1 fold with NE, and was not affected by control siRNA, but was significantly reduced to baseline levels with STAT3-siRNA therapy in the SKOV3 and EG cell lines. Similar groups were examined for MMP production.
  • NE exposure resulted in a 4.8-fold increase in MMP-9 in SKOV3, and a 3.2-fold increase in EG (FIG. 5C).
  • NE lead to a 2.6 and 1.9-fold increase in MMP-2 in SKOV3 and EG, respectively (FIG. 5D).
  • Treatment with STAT3-siRNA prevented NE-induced MMP upregulation in both lines, whereas control siRNA had no effect.
  • FIG. 6 siRNA-directed STAT3 downregulation prevents stress-induced tumor growth.
  • Mice were injected subcutaneously with SKOV3 cells, and 24 hours later treated via intraperitoneal injection with PBS or the f3AR agonist isoproterenol (10 mg/kg). With or without isoproterenol, mice were treated with vehicle, a control nonsilencing siRNA in the delivery liposome DOPC, or STAT3-targeting siRNA in DOPC. After 7 days of treatment, tumor volumes were measures and volume calculated as described in methods. Tumor volume was reduced by 46% with administration of STAT3- specific siRNA in the liposomal delivery vector DOPC, where control siRNA in DOPC did not reduce tumor volume. Isoproterenol administration led to an 8.5-fold increase in tumor volume, which was not affected by treatment with a control siRNA in DOPC, but was almost completely abrogated by S TAT3- specific siRNA in DOPC.
  • STAT proteins are a family of proteins that regulate many aspects of cell growth, survival, and differentiation. They play a dual role in signal transduction and activation of transcription. There are six distinct family members and several isoforms. Cytokine binding induces activation of the intracellular Janus kinase that phosphorylates a specific tyrosine residue in the STAT protein which promotes the dimerization of STAT monomers via their SH 2 domain. The phosphorylated dimer is then actively transported in the nucleus via importin a/b and RanGDP complex.
  • the active STAT dimer binds to cytokine inducible promoter regions of genes containing gamma activated site (GAS) motif and activate transcription of these genes.
  • GAS gamma activated site
  • the STAT protein can be dephosphorylated by nuclear phosphatasess which leads to inactivation of STAT.
  • STAT3 also known as acute phase response factor (APRF)
  • APRF acute phase response factor
  • EGF epidermal growth factor
  • STAT3 has been found to have an important role in signal transduction by interferons.
  • ERK2 induces serine phosphorylation and also associates with STAT3 (Jain et al, 1998).
  • Activation and/or overexpression of STAT3 appears to be involved in cancer.
  • Non-limiting examples of such cancer include myeloma, breast carcinomas, prostate cancer, brain tumors, head and neck carcinomas, melanoma, leukemias, and lymphomas.
  • STAT3 may also play a role in inflammatory diseases, such as rheumatoid arthritis.
  • Activated STAT3 has been found in the synovial fluid of rheumatoid arthritis patients and cells from inflamed joints (Sengupta et al, 1995; Wang et al, 1995).
  • Table 1 lists STATS, and includes GenBank Accession numbers of mRNA sequences from homo sapiens. STATl, STAT2, STAT3, STAT4, STAT5 a and b, and STAT6.
  • NM 003150 transcript SEQ ID NO:5 variant 2
  • RNAi Long double stranded RNA
  • Dicer which is an RNAaseIII family ribonuclease. This process yields siRNAs of -21 nucleotides in length.
  • siRNAs are incorporated into a multiprotein RNA-induced silencing complex (RISC) that is guided to target mRNA. RISC cleaves the target mRNA in the middle of the complementary region.
  • RISC RNA-induced silencing complex
  • miRNAs the related microRNAs (miRNAs) are found that are short RNA fragments (-22 nucleotides).
  • MiRNAs are generated after Dicer-mediated cleavage of longer (-70 nucleotide) precursors with imperfect hairpin RNA structures.
  • the miRNA is incorporated into a miRNA-protein complex (miRNP), which leads to translational repression of target mRNA.
  • miRNP miRNA-protein complex
  • Liposomes are a form of nanoparticles that are attractive carriers for delivering a variety of drugs into the diseased tissue.
  • Optimal liposome size depends on the tumor target. In tumor tissue, the vasculature is discontinuous, and pore sizes vary from 100 to 780 run (Siwak et al. , 2002). By comparison, pore size in normal vascular endothelium is ⁇ 2 nm in most tissues, and 6 nm in post-capillary venules. Most liposomes are 65-125 nm in diameter.
  • Negatively charged liposomes were believed to be more rapidly removed from circulation than neutral or positively charged liposomes; however, recent studies have indicated that the type of negatively charged lipid affects the rate of liposome uptake by the reticuloendothelial system (RES). For example, liposomes containing negatively charged lipids that are not sterically shielded (phosphatidylserine, phosphatidic acid, and phosphatidylglycerol) are cleared more rapidly than neutral liposomes.
  • RES reticuloendothelial system
  • cationic liposomes (1,2-dioleoyl- 3-trimethylammonium-propane [DOTAP]) and cationic-liposome-DNA complexes are more avidly bound and internalized by endothelial cells of angiogenic blood vessels via endocytosis than anionic, neutral, or sterically stabilized neutral liposomes (Thurston et al, 1998; Krasnici et al, 2003).
  • Cationic liposomes may not be ideal delivery vehicles for tumor cells because surface interactions with the tumor cells create an electrostatically derived binding-site barrier effect, inhibiting further association of the delivery systems with tumor spheroids (Kostarelos et al, 2004).
  • siRNA appears to be more stable than antisense molecules, serum nucleases can degrade siRNAs (Leung and Whittaker, 2005).
  • modifications such as chemically stabilized siRNAs with partial phosphorothioate backbone and 2'-0-methyl sugar modifications or boranophosphate siRNAs (Leung and Whittaker, 2005).
  • Elmen and colleagues modified siRNAs with the synthetic RNA-like high affinity nucleotide analogue, Locked Nucleic Acid (LNA), which significantly enhanced the serum half-life of siRNA and stabilized the structure without affecting the gene-silencing capability (Elmen et al, 2005).
  • LNA Locked Nucleic Acid
  • the present invention provides methods and compositions for associating an inhibitory nucleic acid that inhibits the expression of a STAT protein, such as a siNA (e.g., a siRNA) with a lipid and/or liposome.
  • a STAT protein such as a siNA (e.g., a siRNA)
  • siNA e.g., a siRNA
  • the siNA may be encapsulated in the aqueous interior of a liposome, interspersed within the lipid bilayer of a liposome, attached to a liposome via a linking molecule that is associated with both the liposome and the polynucleotide, entrapped in a liposome, complexed with a liposome, dispersed in a solution containing a lipid, mixed with a lipid, combined with a lipid, contained as a suspension in a lipid, contained or complexed with a micelle, or otherwise associated with a lipid.
  • the liposome or liposome/siNA associated compositions of the present invention are not limited to any particular structure in solution. For example, they may be present in a bilayer structure, as micelles, or with a "collapsed" structure. They may also simply be interspersed in a solution, possibly forming aggregates which are not uniform in either size or shape.
  • Lipids are fatty substances which may be naturally occurring or synthetic lipids.
  • lipids include the fatty droplets that naturally occur in the cytoplasm as well as the class of compounds which are well known to those of skill in the art which contain long- chain aliphatic hydrocarbons and their derivatives, such as fatty acids, alcohols, amines, amino alcohols, and aldehydes.
  • DOPC lipid dioleoylphosphatidylcholine
  • Liposome is a generic term encompassing a variety of unilamellar, multilamellar, and multivesicular lipid vehicles formed by the generation of enclosed lipid bilayers or aggregates.
  • Liposomes may be characterized as having vesicular structures with a phospholipid bilayer membrane and an inner aqueous medium.
  • Multilamellar liposomes have multiple lipid layers separated by aqueous medium. They form spontaneously when phospholipids are suspended in an excess of aqueous solution. The lipid components undergo self-rearrangement before the formation of closed structures and entrap water and dissolved solutes between the lipid bilayers (Ghosh and Bachhawat, 1991).
  • the present invention also encompasses compositions that have different structures in solution than the normal vesicular structure.
  • the lipids may assume a micellar structure or merely exist as non-uniform aggregates of lipid molecules.
  • lipofectamine-nucleic acid complexes are also contemplated.
  • Liposome-mediated polynucleotide delivery and expression of foreign DNA in vitro has been very successful.
  • Wong et al (1980) demonstrated the feasibility of liposome- mediated delivery and expression of foreign DNA in cultured chick embryo, HeLa and hepatoma cells.
  • Nicolau et al (1987) accomplished successful liposome-mediated gene transfer in rats after intravenous injection.
  • the lipid may be associated with a hemaglutinating virus (HVJ). This has been shown to facilitate fusion with the cell membrane and promote cell entry of liposome-encapsulated DNA (Kaneda et al, 1989).
  • HVJ hemaglutinating virus
  • the lipid may be complexed or employed in conjunction with nuclear non-histone chromosomal proteins (HMG-I) (Kato et al, 1991).
  • HMG-I nuclear non-histone chromosomal proteins
  • the lipid may be complexed or employed in conjunction with both HVJ and HMG-I. In that such expression vectors have been successfully employed in transfer of a polynucleotide in vitro and in vivo, then they are applicable for the present invention.
  • Neutral liposomes or lipid composition or “non-charged liposomes or lipid composition,” as used herein, are defined as liposomes or lipid compositions having one or more lipids that yield an essentially-neutral, net charge (substantially non-charged).
  • essentially neutral or “essentially non-charged” it is meant that few, if any, lipids within a given population ⁇ e.g., a population of liposomes) include a charge that is not canceled by an opposite charge of another component ⁇ e.g., fewer than 10% of components include a non- canceled charge, more preferably fewer than 5%, and most preferably fewer than 1%).
  • a composition may be prepared wherein the lipid component of the composition is essentially neutral but is not in the form of liposomes.
  • neutral liposomes or lipid compositions may include mostly lipids and/or phospholipids that are themselves neutral.
  • amphipathic lipids may be incorporated into or used to generate neutral liposomes or lipid compositions.
  • a neutral liposome may be generated by combining positively and negatively charged lipids so that those charges substantially cancel one another.
  • few, if any, charged lipids are present whose charge is not canceled by an oppositely-charged lipid ⁇ e.g., fewer than 10% of charged lipids have a charge that is not canceled, more preferably fewer than 5%, and most preferably fewer than 1%).
  • the above approach may be used to generate a neutral lipid composition wherein the lipid component of the composition is not in the form of liposomes.
  • a neutral liposome may be used to deliver a siRNA.
  • the neutral liposome may contain a siRNA directed to the suppression of translation of a single gene, or the neutral liposome may contain multiple siRNA that are directed to the suppression of translation of multiple genes.
  • the neutral liposome may also contain a chemotherapeutic in addition to the siRNA; thus, in certain embodiments, chemotherapeutic and a siRNA may be delivered to a cell (e.g., a cancerous cell in a human subject) in the same or separate compositions.
  • Lipid compositions of the present invention may comprise phospholipids.
  • a single kind or type of phospholipid may be used in the creation of lipid compositions such as liposomes (e.g., DOPC used to generate neutral liposomes).
  • more than one kind or type of phospholipid may be used.
  • Phospholipids include glycerophospholipids and certain sphingolipids.
  • Phospholipids include, but are not limited to, dioleoylphosphatidylycholine ("DOPC"), egg phosphatidylcholine (“EPC”), dilauryloylphosphatidylcholine (“DLPC”), dimyristoylphosphatidylcholine (“DMPC”), dipalmitoylphosphatidylcholine (“DPPC”), distearoylphosphatidylcholine (“DSPC”), l-myristoyl-2-palmitoyl phosphatidylcholine (“MPPC”), l-palmitoyl-2-myristoyl phosphatidylcholine (“PMPC”), l-palmitoyl-2-stearoyl phosphatidylcholine (“PSPC”), 1 -stearoyl-2-palmitoyl phosphatidylcholine (“SPPC”), dil
  • Phospholipids include, for example, phosphatidylcholines, phosphatidylglycerols, and phosphatidylethanolamines; because phosphatidylethanolamines and phosphatidyl cholines are non-charged under physiological conditions (i.e., at about pH 7), these compounds may be particularly useful for generating neutral liposomes.
  • the phospholipid DOPC is used to produce non-charged liposomes or lipid compositions.
  • a lipid that is not a phospholipid e.g., a cholesterol
  • Phospholipids may be from natural or synthetic sources. However, phospholipids from natural sources, such as egg or soybean phosphatidylcholine, brain phosphatidic acid, brain or plant phosphatidylinositol, heart cardiolipin and plant or bacterial phosphatidylethanolamine are not used in certain embodiments as the primary phosphatide (i.e., constituting 50% or more of the total phosphatide composition) because this may result in instability and leakiness of the resulting liposomes.
  • natural sources such as egg or soybean phosphatidylcholine, brain phosphatidic acid, brain or plant phosphatidylinositol, heart cardiolipin and plant or bacterial phosphatidylethanolamine are not used in certain embodiments as the primary phosphatide (i.e., constituting 50% or more of the total phosphatide composition) because this may result in instability and leakiness of the resulting liposomes.
  • Liposomes and lipid compositions of the present invention can be made by different methods.
  • a nucleotide e.g., siRNA
  • a nucleotide may be encapsulated in a neutral liposome using a method involving ethanol and calcium (Bailey and Sullivan, 2000).
  • the size of the liposomes varies depending on the method of synthesis.
  • a liposome suspended in an aqueous solution is generally in the shape of a spherical vesicle, and may have one or more concentric layers of lipid bilayer molecules. Each layer consists of a parallel array of molecules represented by the formula XY, wherein X is a hydrophilic moiety and Y is a hydrophobic moiety.
  • the concentric layers are arranged such that the hydrophilic moieties tend to remain in contact with an aqueous phase and the hydrophobic regions tend to self-associate.
  • the lipid molecules may form a bilayer, known as a lamella, of the arrangement XY-YX. Aggregates of lipids may form when the hydrophilic and hydrophobic parts of more than one lipid molecule become associated with each other. The size and shape of these aggregates will depend upon many different variables, such as the nature of the solvent and the presence of other compounds in the solution.
  • Lipids suitable for use according to the present invention can be obtained from commercial sources.
  • DMPC dimyristyl phosphatidylcholine
  • DCP dicetyl phosphate
  • DMPG dimyristyl phosphatidylglycerol
  • liposomes within the scope of the present invention can be prepared in accordance with known laboratory techniques.
  • liposomes are prepared by mixing liposomal lipids, in a solvent in a container (e.g., a glass, pear-shaped flask).
  • a container e.g., a glass, pear-shaped flask.
  • the container will typically have a volume ten-times greater than the volume of the expected suspension of liposomes.
  • the solvent may be removed at approximately 4O 0 C under negative pressure.
  • the solvent may be removed within about 5 minutes to 2 hours, depending on the desired volume of the liposomes.
  • the composition can be dried further in a desiccator under vacuum.
  • Dried lipids can be hydrated at approximately 25-50 mM phospholipid in sterile, pyrogen-free water by shaking until all the lipid film is resuspended.
  • the aqueous liposomes can be then separated into aliquots, each placed in a vial, lyophilized and sealed under vacuum.
  • Liposomes can also be prepared in accordance with other known laboratory procedures: the method of Bangham et al. (1965), the contents of which are incorporated herein by reference; the method of Gregoriadis, as described in DRUG CARRIERS IN BIOLOGY AND MEDICINE (1979), the contents of which are incorporated herein by reference; the method of Deamer and Uster (1983), the contents of which are incorporated by reference; and the reverse-phase evaporation method as described by Szoka and Papahadjopoulos (1978).
  • the aforementioned methods differ in their respective abilities to entrap aqueous material and their respective aqueous space-to-lipid ratios.
  • Dried lipids or lyophilized liposomes may be dehydrated and reconstituted in a solution of inhibitory peptide and diluted to an appropriate concentration with a suitable solvent (e.g., DPBS). The mixture may then be vigorously shaken in a vortex mixer. Unencapsulated nucleic acid may be removed by centrifugation at 29,00Og and the liposomal pellets washed. The washed liposomes may be resuspended at an appropriate total phospholipid concentration (e.g., about 50-200 mM). The amount of nucleic acid encapsulated can be determined in accordance with standard methods. After determination of the amount of nucleic acid encapsulated in the liposome preparation, the liposomes may be diluted to appropriate concentrations and stored at 4°C until use.
  • a suitable solvent e.g., DPBS
  • siNA e.g., siRNA
  • siRNA and double- stranded RNA have been described in U.S. Patents 6,506,559 and 6,573,099, as well as in U.S. Patent Applications 2003/0051263, 2003/0055020, 2004/0265839, 2002/0168707, 2003/0159161, and 2004/0064842, all of which are herein incorporated by reference in their entirety.
  • a siNA may comprise a nucleotide and a nucleic acid or nucleotide analog.
  • siNA form a double-stranded structure; the double-stranded structure may result from two separate nucleic acids that are partially or completely complementary.
  • the siNA may comprise only a single nucleic acid (polynucleotide) or nucleic acid analog and form a double-stranded structure by complementing with itself (e.g., forming a hairpin loop).
  • the double-stranded structure of the siNA may comprise 16, 20, 25, 30, 35, 40, 45, 50, 60, 65, 70, 75, 80, 85, 90 to 100, 150, 200, 250, 300, 350, 400, 450, 500 or more contiguous nucleobases, including all ranges therebetween.
  • the siNA may comprise 17 to 35 contiguous nucleobases, more preferably 18 to 30 contiguous nucleobases, more preferably 19 to 25 nucleobases, more preferably 20 to 23 contiguous nucleobases, or 20 to 22 contiguous nucleobases, or 21 contiguous nucleobases that hybridize with a complementary nucleic acid (which may be another part of the same nucleic acid or a separate complementary nucleic acid) to form a double-stranded structure.
  • a complementary nucleic acid which may be another part of the same nucleic acid or a separate complementary nucleic acid
  • Agents of the present invention useful for practicing the methods of the present invention include, but are not limited to siRNAs.
  • dsRNA double-stranded RNA
  • siRNA small interfering RNA
  • RNAi RNA interference
  • RNA interference has been referred to as “cosuppression,” “post-transcriptional gene silencing,” “sense suppression,” and “quelling.”
  • RNAi is an attractive biotechnological tool because it provides a means for knocking out the activity of specific genes.
  • RNAi there are several factors that need to be considered such as the nature of the siRNA, the durability of the silencing effect, and the choice of delivery system.
  • the siRNA that is introduced into the organism will typically contain exonic sequences.
  • the RNAi process is homology dependent, so the sequences must be carefully selected so as to maximize gene specificity, while minimizing the possibility of cross-interference between homologous, but not gene-specific sequences.
  • the siRNA exhibits greater than 80, 85, 90, 95, 98,% or even 100% identity between the sequence of the siRNA and the gene to be inhibited. Sequences less than about 80% identical to the target gene are substantially less effective.
  • the greater homology between the siRNA and the STAT gene to be inhibited the less likely expression of unrelated genes will be affected.
  • the size of the siRNA is an important consideration.
  • the present invention relates to siRNA molecules that include at least about 19-25 nucleotides, and are able to modulate the STAT gene expression.
  • the siRNA is preferably less than 500, 200, 100, 50 or 25 nucleotides in length. More preferably, the siRNA is from about 19 nucleotides to about 25 nucleotides in length.
  • siRNA can be obtained from commercial sources, natural sources, or can be synthesized using any of a number of techniques well-known to those of ordinary skill in the art.
  • one commercial source of predesigned siRNA is Ambion®, Austin, TX.
  • An inhibitory nucleic acid that can be applied in the compositions and methods of the present invention may be any nucleic acid sequence that has been found by any source to be a validated downregulator of a STAT protein.
  • the inhibitory nucleic acid is Qiagen® (Valencia, CA) validated siRNA product Catalog Number SIO2662338.
  • the invention generally features an isolated siRNA molecule of at least 19 nucleotides, having at least one strand that is substantially complementary to at least ten but no more than thirty consecutive nucleotides of a nucleic acid that encodes a STAT protein, and that reduces the expression of the STAT protein.
  • the siRNA molecule has at least one strand that is substantially complementary to at least ten but no more than thirty consecutive nucleotides of the mRNA that encodes STAT3.
  • the siRNA molecule is at least 75, 80, 85, or 90% homologous, preferably 95%, 99%, or 100% homologous, to at least 10 contiguous nucleotides of any of the nucleic acid sequences encoding a full-length STAT protein, such as those in Table 1.
  • the siRNA may also comprise an alteration of one or more nucleotides.
  • Such alterations can include the addition of non-nucleotide material, such as to the end(s) of the 19 to 25 nucleotide RNA or internally (at one or more nucleotides of the RNA).
  • the RNA molecule contains a 3'-hydroxyl group.
  • Nucleotides in the RNA molecules of the present invention can also comprise non-standard nucleotides, including non-naturally occurring nucleotides or deoxyribonucleotides.
  • the double-stranded oligonucleotide may contain a modified backbone, for example, phosphorothioate, phosphorodithioate, or other modified backbones known in the art, or may contain non-natural internucleoside linkages.
  • Additional modifications of siRNAs e.g., 2'-O-methyl ribonucleotides, 2'-deoxy-2'-fluoro ribonucleotides, "universal base" nucleotides, 5-C-methyl nucleotides, one or more phosphorothioate internucleotide linkages, and inverted deoxyabasic residue incorporation
  • U.S. Application Publication 20040019001 and U.S. Patent 6,673,611 each of which is incorporated by referencein its entirety.
  • RNAi is capable of decreasing the expression of a STAT protein, such as STAT3, by at least 10%, 20%, 30%, or 40%, more preferably by at least 50%, 60%, or 70%, and most preferably by at least 75%, 80%, 90%, 95% or more.
  • Certain embodiments of the present invention pertain to methods of inhibiting expression of a gene encoding a STAT protein in a cell.
  • the STAT protein is STAT3.
  • Introduction of siRNA into cells can be achieved by methods known in the art, including for example, microinjection, electroporation, or transfection of a vector comprising a nucleic acid from which the siRNA can be transcribed.
  • a siRNA can be directly introduced into a cell in a form that is capable of binding to target mRNA transcripts.
  • the siRNA may be combined or modified with liposomes, poly-L-lysine, lipids, cholesterol, lipofectine or derivatives thereof.
  • cholesterol-conjugated siRNA can be used (see, Song et al, 2003).
  • the present invention provides methods and compositions for the delivery of siNA via neutral liposomes. Because a siNA is composed of a nucleic acid, methods relating to nucleic acids (e.g., production of a nucleic acid, modification of a nucleic acid, etc.) may also be used with regard to a siNA.
  • nucleic acid is well known in the art.
  • a “nucleic acid” as used herein will generally refer to a molecule (i.e., a strand) of DNA, RNA or a derivative or analog thereof, comprising a nucleobase.
  • a nucleobase includes, for example, a naturally occurring purine or pyrimidine base found in DNA (e.g., an adenine "A,” a guanine "G,” a thymine “T” or a cytosine "C”) or RNA (e.g., an A, a G, an uracil "U” or a C).
  • nucleic acid encompass the terms “oligonucleotide” and “polynucleotide,” each as a subgenus of the term “nucleic acid.”
  • oligonucleotide refers to a molecule of between 3 and about 100 nucleobases in length.
  • polynucleotide refers to at least one molecule of greater than about 100 nucleobases in length.
  • a nucleic acid may encompass a double-stranded molecule that comprises one or more complementary strand(s) or "complement(s)" of a particular sequence, typically comprising a molecule.
  • a single stranded nucleic acid may be denoted by the prefix "ss” and a double stranded nucleic acid by the prefix "ds”.
  • antisense oligonucleotides targeted to nucleic acids encoding STAT3 can be found in U.S. Patent Application Pub. No. 20010029250, herein specifically incorporated by reference. Particular examples include SEQ ID NOs: 11 -90. 1. Nucleobases
  • nucleobase refers to a heterocyclic base, such as for example a naturally occurring nucleobase (i.e., an A, T, G, C or U) found in at least one naturally occurring nucleic acid (i.e., DNA and RNA), and naturally or non-naturally occurring derivative(s) and analogs of such a nucleobase.
  • a nucleobase generally can form one or more hydrogen bonds (“anneal” or “hybridize”) with at least one naturally occurring nucleobase in manner that may substitute for naturally occurring nucleobase pairing (e.g., the hydrogen bonding between A and T, G and C, and A and U).
  • Purine and/or "pyrimidine” nucleobase(s) encompass naturally occurring purine and/or pyrimidine nucleobases and also derivative(s) and analog(s) thereof, including but not limited to, those a purine or pyrimidine substituted by one or more of an alkyl, caboxyalkyl, amino, hydroxyl, halogen (i.e., fluoro, chloro, bromo, or iodo), thiol or alkylthiol moeity.
  • halogen i.e., fluoro, chloro, bromo, or iodo
  • Preferred alkyl (e.g., alkyl, caboxyalkyl, etc.) moeities comprise of from about 1, about 2, about 3, about 4, about 5, to about 6 carbon atoms.
  • a nucleobase may be comprised in a nucleside or nucleotide, using any chemical or natural synthesis method described herein or known to one of ordinary skill in the art.
  • nucleoside refers to an individual chemical unit comprising a nucleobase covalently attached to a nucleobase linker moiety.
  • nucleobase linker moiety is a sugar comprising 5-carbon atoms (i.e., a "5-carbon sugar"), including but not limited to a deoxyribose, a ribose, an arabinose, or a derivative or an analog of a 5-carbon sugar.
  • Non-limiting examples of a derivative or an analog of a 5-carbon sugar include a 2'-fluoro-2'-deoxyribose or a carbocyclic sugar where a carbon is substituted for an oxygen atom in the sugar ring.
  • a nucleoside comprising a purine (i.e., A or G) or a 7-deazapurine nucleobase typically covalently attaches the 9 position of a purine or a 7-deazapurine to the 1 '-position of a 5-carbon sugar.
  • a nucleoside comprising a pyrimidine nucleobase typically covalently attaches a 1 position of a pyrimidine to a l'-position of a 5-carbon sugar (Kornberg and Baker, 1992).
  • nucleotide refers to a nucleoside further comprising a "backbone moiety".
  • a backbone moiety generally covalently attaches a nucleotide to another molecule comprising a nucleotide, or to another nucleotide to form a nucleic acid.
  • the "backbone moiety” in naturally occurring nucleotides typically comprises a phosphorus moiety, which is covalently attached to a 5 -carbon sugar. The attachment of the backbone moiety typically occurs at either the 3'- or 5'-position of the 5-carbon sugar.
  • other types of attachments are known in the art, particularly when a nucleotide comprises derivatives or analogs of a naturally occurring 5-carbon sugar or phosphorus moiety. 4. Nucleic Acid Analogs
  • a nucleic acid may comprise, or be composed entirely of, a derivative or analog of a nucleobase, a nucleobase linker moiety and/or backbone moiety that may be present in a naturally occurring nucleic acid.
  • a "derivative” refers to a chemically modified or altered form of a naturally occurring molecule
  • the terms “mimic” or “analog” refer to a molecule that may or may not structurally resemble a naturally occurring molecule or moiety, but possesses similar functions.
  • a “moiety” generally refers to a smaller chemical or molecular component of a larger chemical or molecular structure. Nucleobase, nucleoside and nucleotide analogs or derivatives are well known in the art, and have been described (see for example, Scheit, 1980, incorporated herein by reference).
  • nucleosides, nucleotides, or nucleic acids comprising 5-carbon sugar and/or backbone moiety derivatives or analogs include those in U.S. Patent 5,681,947 which describes oligonucleotides comprising purine derivatives that form triple helixes with and/or prevent expression of dsDNA; U.S. Patents 5,652,099 and 5,763,167 which describe nucleic acids incorporating fluorescent analogs of nucleosides found in DNA or RNA, particularly for use as flourescent nucleic acids probes; U.S. Patent 5,614,617 which describes oligonucleotide analogs with substitutions on pyrimidine rings that possess enhanced nuclease stability; U.S.
  • Patents 5,670,663, 5,872,232 and 5,859,221 which describe oligonucleotide analogs with modified 5-carbon sugars (i.e., modified T- deoxyfuranosyl moieties) used in nucleic acid detection;
  • U.S. Patent 5,446,137 which describes oligonucleotides comprising at least one 5-carbon sugar moiety substituted at the 4' position with a substituent other than hydrogen that can be used in hybridization assays;
  • U.S. Patent 5,886,165 which describes oligonucleotides with both deoxyribonucleotides with 3'-5' intemucleotide linkages and ribonucleotides with 2'-5' internucleotide linkages;
  • Patent 5,714,606 which describes a modified internucleotide linkage wherein a 3 '-position oxygen of the internucleotide linkage is replaced by a carbon to enhance the nuclease resistance of nucleic acids;
  • U.S. Patent 5,672,697 which describes oligonucleotides containing one or more 5' methylene phosphonate internucleotide linkages that enhance nuclease resistance;
  • U.S. Patents 5,466,786 and 5,792,847 which describe the linkage of a substituent moeity which may comprise a drug or label to the 2' carbon of an oligonucleotide to provide enhanced nuclease stability and ability to deliver drugs or detection moieties;
  • Patent 5,223,618 which describes oligonucleotide analogs with a 2 or 3 carbon backbone linkage attaching the 4' position and 3' position of adjacent 5-carbon sugar moiety to enhanced cellular uptake, resistance to nucleases and hybridization to target RNA;
  • Patent 5,470,967 which describes oligonucleotides comprising at least one sulfamate or sulfamide internucleotide linkage that are useful as nucleic acid hybridization probe;
  • Patents 5,378,825, 5,777,092, 5,623,070, 5,610,289 and 5,602,240 which describe oligonucleotides with three or four atom linker moeity replacing phosphodiester backbone moeity used for improved nuclease resistance, cellular uptake and regulating RNA expression
  • U.S. Patent 5,858,988 which describes hydrophobic carrier agent attached to the 2'-0 position of oligonuceotides to enhanced their membrane permeability and stability
  • U.S. Patent 5,214,136 which describes olignucleotides conjugated to anthraquinone at the 5' terminus that possess enhanced hybridization to DNA or RNA; enhanced stability to nucleases;
  • Patent 5,700,922 which describes PNA-DNA-PNA chimeras wherein the DNA comprises 2'-deoxy-erythro- pentofuranosyl nucleotides for enhanced nuclease resistance, binding affinity, and ability to activate RNase H; and U.S. Patent 5,708,154 which describes RNA linked to a DNA to form a DNA-RNA hybrid.
  • a nucleic acid comprising a derivative or analog of a nucleoside or nucleotide may be used in the methods and compositions of the invention.
  • a non-limiting example is a "polyether nucleic acid", described in U.S. Patent 5,908,845, incorporated herein by reference.
  • polyether nucleic acid one or more nucleobases are linked to chiral carbon atoms in a polyether backbone.
  • peptide nucleic acid also known as a "PNA”, “peptide-based nucleic acid analog” or "PENAM”, described in U.S. Patent 5,786,461, 5891,625, 5,773,571, 5,766,855, 5,736,336, 5,719,262, 5,714,331, 5,539,082, and WO 92/20702, each of which is incorporated herein by reference.
  • Peptide nucleic acids generally have enhanced sequence specificity, binding properties, and resistance to enzymatic degradation in comparison to molecules such as DNA and RNA (Egholm et al, 1993; PCT/EP/01219).
  • a peptide nucleic acid generally comprises one or more nucleotides or nucleosides that comprise a nucleobase moiety, a nucleobase linker moeity that is not a 5- carbon sugar, and/or a backbone moiety that is not a phosphate backbone moiety.
  • nucleobase linker moieties described for PNAs include aza nitrogen atoms, amido and/or ureido tethers (see for example, U.S. Patent 5,539,082).
  • backbone moieties described for PNAs include an aminoethylglycine, polyamide, polyethyl, polythioamide, polysulf ⁇ namide or polysulfonamide backbone moiety.
  • a nucleic acid analogue such as a peptide nucleic acid may be used to inhibit nucleic acid amplification, such as in PCRTM, to reduce false positives and discriminate between single base mutants, as described in U.S. Patent 5,891,625.
  • nucleic acid amplification such as in PCRTM
  • Other modifications and uses of nucleic acid analogs are known in the art, and it is anticipated that these techniques and types of nucleic acid analogs may be used with the present invention.
  • U.S. Patent 5,786,461 describes PNAs with amino acid side chains attached to the PNA backbone to enhance solubility of the molecule.
  • the cellular uptake property of PNAs is increased by attachment of a lipophilic group.
  • a nucleic acid may be made by any technique known to one of ordinary skill in the art, such as chemical synthesis, enzymatic production or biological production.
  • a synthetic nucleic acid ⁇ e.g., a synthetic oligonucleotide
  • Non-limiting examples of a synthetic nucleic acid include a nucleic acid made by in vitro chemically synthesis using phosphotriester, phosphite or phosphoramidite chemistry and solid phase techniques such as described in EP 266,032, incorporated herein by reference, or via deoxynucleoside H-phosphonate intermediates as described by Froehler et al, 1986 and U.S. Patent 5,705,629, each incorporated herein by reference.
  • one or more oligonucleotide may be used.
  • Various different mechanisms of oligonucleotide synthesis have been disclosed in for example, U.S. Patents 4,659,774, 4,816,571, 5,141,813, 5,264,566, 4,959,463, 5,428,148, 5,554,744, 5,574,146, 5,602,244, each of which is incorporated herein by reference.
  • a non-limiting example of an enzymatically produced nucleic acid include one produced by enzymes in amplification reactions such as PCRTM (see for example, U.S. Patent
  • a non-limiting example of a biologically produced nucleic acid includes a recombinant nucleic acid produced (i.e., replicated) in a living cell, such as a recombinant DNA vector replicated in bacteria (see for example, Sambrook et al. 2001, incorporated herein by reference).
  • a nucleic acid may be purified on polyacrylamide gels, cesium chloride centrifugation gradients, or by any other means known to one of ordinary skill in the art (see for example, Sambrook et al, 2001, incorporated herein by reference).
  • the present invention concerns a nucleic acid that is an isolated nucleic acid.
  • isolated nucleic acid refers to a nucleic acid molecule (e.g., an RNA or DNA molecule) that has been isolated free of, or is otherwise free of, the bulk of the total genomic and transcribed nucleic acids of one or more cells.
  • isolated nucleic acid refers to a nucleic acid that has been isolated free of, or is otherwise free of, bulk of cellular components or in vitro reaction components such as for example, macromolecules such as lipids or proteins, small biological molecules, and the like.
  • hybridization As used herein, “hybridization”, “hybridizes” or “capable of hybridizing” is understood to mean the forming of a double or triple stranded molecule or a molecule with partial double or triple stranded nature.
  • hybridize as used herein is synonymous with “hybridize.”
  • hybridization encompasses the terms “stringent condition(s)” or “high stringency” and the terms “low stringency” or “low stringency condition(s).”
  • stringent condition(s) or “high stringency” are those conditions that allow hybridization between or within one or more nucleic acid strand(s) containing complementary sequence(s), but precludes hybridization of random sequences. Stringent conditions tolerate little, if any, mismatch between a nucleic acid and a target strand.
  • Non-limiting applications include isolating a nucleic acid, such as a gene or a nucleic acid segment thereof, or detecting at least one specific mRNA transcript or a nucleic acid segment thereof, and the like.
  • Stringent conditions may comprise low salt and/or high temperature conditions, such as provided by about 0.02 M to about 0.15 M NaCl at temperatures of about 50 0 C to about 70°C. It is understood that the temperature and ionic strength of a desired stringency are determined in part by the length of the particular nucleic acid(s), the length and nucleobase content of the target sequence(s), the charge composition of the nucleic acid(s), and to the presence or concentration of formamide, tetramethylammonium chloride or other solvent(s) in a hybridization mixture.
  • low stringency or “low stringency conditions”
  • non-limiting examples of low stringency include hybridization performed at about 0.15 M to about 0.9 M NaCl at a temperature range of about 20 0 C to about 50 0 C.
  • hybridization performed at about 0.15 M to about 0.9 M NaCl at a temperature range of about 20 0 C to about 50 0 C.
  • Treatment refers to administration or application of a therapeutic agent to a subject or performance of a procedure or modality on a subject for the purpose of obtaining a therapeutic benefit of a disease or health-related condition.
  • a treatment may include administration of a pharmaceutically effective amount of a nucleic acid that inhibits the expression of a gene that encodes a STAT and a neutral lipid for the purposes of minimizing the growth or invasion of a tumor.
  • a "subject” refers to either a human or non-human, such as primates, mammals, and vertebrates. In particular embodiments, the subject is a human.
  • the term "therapeutic benefit” or “therapeutically effective” as used throughout this application refers to anything that promotes or enhances the well-being of the subject with respect to the medical treatment of this condition. This includes, but is not limited to, a reduction in the frequency or severity of the signs or symptoms of a disease.
  • treatment of cancer may involve, for example, a reduction in the size of a tumor, a reduction in the invasiveness of a tumor, reduction in the growth rate of the cancer, or prevention of metastasis.
  • a "disease” or "health-related condition” can be any pathological condition of a body part, an organ, or a system resulting from any cause, such as infection, genetic defect, and/or environmental stress. The cause may or may not be known.
  • the methods include identifying a patient in need of treatment.
  • a patient may be identified, for example, based on taking a patient history, based on findings on clinical examination, based on health screenings, or by self- referral.
  • the present invention may be used to treat any disease associated with increased expression of a STAT protein.
  • the disease may be a hyperproliferative disease, such as cancer.
  • a siRNA that binds to a nucleic acid that encodes a STAT3 protein may be administered to treat a cancer.
  • the cancer may be a solid tumor, metastatic cancer, or non-metastatic cancer.
  • the cancer may originate in the bladder, blood, bone, bone marrow, brain, breast, colon, esophagus, gastrointestine, gum, head, kidney, liver, lung, nasopharynx, neck, ovary, prostate, skin, stomach, testis, tongue, or uterus.
  • the cancer is human ovarian cancer.
  • the cancer may specifically be of the following histological type, though it is not limited to these: neoplasm, malignant; carcinoma; carcinoma, undifferentiated; giant and spindle cell carcinoma; small cell carcinoma; papillary carcinoma; squamous cell carcinoma; lymphoepithelial carcinoma; basal cell carcinoma; pilomatrix carcinoma; transitional cell carcinoma; papillary transitional cell carcinoma; adenocarcinoma; gastrinoma, malignant; cholangiocarcinoma; hepatocellular carcinoma; combined hepatocellular carcinoma and cholangiocarcinoma; trabecular adenocarcinoma; adenoid cystic carcinoma; adenocarcinoma in adenomatous polyp; adenocarcinoma, familial polyposis coli; solid carcinoma; carcinoid tumor, malignant; branchiolo-alveolar adenocarcinoma; papillary adenocarcinoma; chromophobe carcinoma; acid
  • the cancer is ovarian cancer.
  • the ovarian cancer may be an epithelial tumor (e.g., serous, endometrioid, mucinous, and clear cell tumors), a germ cell tumor, a sex cord-stormal cell tumor, a Brenner tumor, an undifferentiated tumor, or a transitional cell tumor.
  • the term "ovarian cancer” also includes tumors that are adjacent to ovarian tissue, such as extraovarian peritoneal carcinoma (intraperitoneal carcinomatosis).
  • the ovarian tumor may be benign, malignant, or pre- malignant.
  • the disease may also be an inflammatory disease. Non-limiting examples of inflammatory diseases include infectious diseases, arthritis, and collagen-vascular diseases. In a specific embodiment, the disease is rheumatoid arthritis.
  • lipid complex As a pharmaceutical composition appropriate for the intended application, it will generally be beneficial to prepare the lipid complex as a pharmaceutical composition appropriate for the intended application. This will typically entail preparing a pharmaceutical composition that is essentially free of pyrogens, as well as any other impurities that could be harmful to humans or animals. One may also employ appropriate buffers to render the complex stable and allow for uptake by target cells.
  • pharmaceutical or pharmacologically acceptable refers to molecular entities and compositions that do not produce an adverse, allergic or other untoward reaction when administered to an animal, such as a human, as appropriate.
  • compositions that contains at least one non-charged lipid component comprising a siNA or additional active ingredient will be known to those of skill in the art in light of the present disclosure, as exemplified by Remington: The Science and Practice of Pharmacy, 21st, 2005, incorporated herein by reference. Moreover, for animal (e.g., human) administration, it will be understood that preparations should meet sterility, pyrogenicity, general safety and purity standards as required by FDA Office of Biological Standards.
  • pharmaceutically acceptable carrier includes any and all solvents, dispersion media, coatings, surfactants, antioxidants, preservatives (e.g., antibacterial agents, antifungal agents), isotonic agents, absorption delaying agents, salts, preservatives, drugs, drug stabilizers, gels, binders, excipients, disintegration agents, lubricants, sweetening agents, flavoring agents, dyes, such like materials and combinations thereof, as would be known to one of ordinary skill in the art.
  • preservatives e.g., antibacterial agents, antifungal agents
  • isotonic agents e.g., absorption delaying agents, salts, preservatives, drugs, drug stabilizers, gels, binders, excipients, disintegration agents, lubricants, sweetening agents, flavoring agents, dyes, such like materials and combinations thereof, as would be known to one of ordinary skill in the art.
  • a pharmaceutically acceptable carrier is preferably formulated for administration to a human, although in certain embodiments it may be desirable to use a pharmaceutically acceptable carrier that is formulated for administration to a non-human animal but which would not be acceptable (e.g., due to governmental regulations) for administration to a human. Except insofar as any conventional carrier is incompatible with the active ingredient, its use in the therapeutic or pharmaceutical compositions is contemplated.
  • compositions of the present invention administered to a patient or subject can be determined by physical and physiological factors such as body weight, severity of condition, the type of disease being treated, previous or concurrent therapeutic interventions, idiopathy of the patient and on the route of administration.
  • the practitioner responsible for administration will, in any event, determine the concentration of active ingredient(s) in a composition and appropriate dose(s) for the individual subject.
  • pharmaceutical compositions may comprise, for example, at least about 0.1% of an active compound.
  • the an active compound may comprise between about 2% to about 75% of the weight of the unit, or between about 25% to about 60%, for example, and any range derivable therein.
  • a dose may also comprise from about 1 microgram/kg/body weight, about 5 microgram/kg/body weight, about 10 microgram/kg/body weight, about 50 microgram/kg/body weight, about 100 microgram/kg/body weight, about 200 microgram/kg/body weight, about 350 microgram/kg/body weight, about 500 microgram/kg/body weight, about 1 milligram/kg/body weight, about 5 milligram/kg/body weight, about 10 milligram/kg/body weight, about 50 milligram/kg/body weight, about 100 milligram/kg/body weight, about 200 milligram/kg/body weight, about 350 milligram/kg/body weight, about 500 milligram/kg/body weight, to about 1000 mg/kg/body weight or more per administration, and any range derivable therein.
  • a range of about 5 ⁇ g/kg/body weight to about 100 mg/kg/body weight, about 5 microgram/kg/body weight to about 500 milligram/kg/body weight, etc., can be administered.
  • a gene expression inhibitor may be administered in a dose of 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 15, 20, 25, 30, 40, 50, 60, 70, 80, 90, 100 or more ⁇ g of nucleic acid per dose.
  • Each dose may be in a volume of 1, 10, 50, 100, 200, 500, 1000 or more ⁇ l or ml.
  • Solutions of therapeutic compositions can be prepared in water suitably mixed with a surfactant, such as hydroxypropylcellulose.
  • Dispersions also can be prepared in glycerol, liquid polyethylene glycols, mixtures thereof and in oils. Under ordinary conditions of storage and use, these preparations contain a preservative to prevent the growth of microorganisms.
  • compositions of the present invention are advantageously administered in the form of injectable compositions either as liquid solutions or suspensions; solid forms suitable for solution in, or suspension in, liquid prior to injection may also be prepared. These preparations also may be emulsified.
  • a typical composition for such purpose comprises a pharmaceutically acceptable carrier.
  • the composition may contain 10 mg, 25 mg, 50 mg or up to about 100 mg of human serum albumin per milliliter of phosphate buffered saline.
  • Other pharmaceutically acceptable carriers include aqueous solutions, non-toxic excipients, including salts, preservatives, buffers and the like.
  • non-aqueous solvents examples include propylene glycol, polyethylene glycol, vegetable oil and injectable organic esters such as ethyloleate.
  • Aqueous carriers include water, alcoholic/aqueous solutions, saline solutions, parenteral vehicles such as sodium chloride, Ringer's dextrose, etc.
  • Intravenous vehicles include fluid and nutrient replenishers.
  • Preservatives include antimicrobial agents, anti-oxidants, chelating agents and inert gases.
  • the pH and exact concentration of the various components the pharmaceutical composition are adjusted according to well known parameters.
  • Oral formulations include such typical excipients as, for example, pharmaceutical grades of mannitol, lactose, starch, magnesium stearate, sodium saccharine, cellulose, magnesium carbonate and the like.
  • the compositions take the form of solutions, suspensions, tablets, pills, capsules, sustained release formulations or powders.
  • the therapeutic compositions of the present invention may include classic pharmaceutical preparations. Administration of therapeutic compositions according to the present invention will be via any common route so long as the target tissue is available via that route. This includes oral, nasal, buccal, rectal, vaginal or topical. Topical administration may be particularly advantageous for the treatment of skin cancers, to prevent chemotherapy- induced alopecia or other dermal hyperproliferative disorder. Alternatively, administration may be by orthotopic, intradermal, subcutaneous, intramuscular, intraperitoneal or intravenous injection. Such compositions would normally be administered as pharmaceutically acceptable compositions that include physiologically acceptable carriers, buffers or other excipients. For treatment of conditions of the lungs, or respiratory tract, aerosol delivery can be used.
  • volume of the aerosol is between about 0.01 ml and 0.5 ml.
  • An effective amount of the therapeutic composition is determined based on the intended goal.
  • the term "unit dose” or “dosage” refers to physically discrete units suitable for use in a subject, each unit containing a predetermined-quantity of the therapeutic composition calculated to produce the desired responses discussed above in association with its administration, i.e., the appropriate route and treatment regimen.
  • Precise amounts of the therapeutic composition also depend on the judgment of the practitioner and are peculiar to each individual. Factors affecting the dose include the physical and clinical state of the patient, the route of administration, the intended goal of treatment (e.g., alleviation of symptoms versus cure) and the potency, stability and toxicity of the particular therapeutic substance.
  • compositions and methods of the present invention involve an inhibitor of expression of a STAT protein, or construct capable of expressing an inhibitor of STAT expression, in combination with a second or additional therapy.
  • Such therapy can be applied in the treatment of any disease that is associated with increased expression or activity of a STAT protein.
  • the disease may be an inflammatory disease or a hyperproliferative disease, such as cancer.
  • Non-limiting examples of inflammatory diseases contemplated by the present invention include rheumatoid arthritis and infectious disease.
  • compositions including combination therapies enhance the therapeutic or protective effect, and/or increase the therapeutic effect of another anti-cancer or anti-hyperproliferative therapy.
  • Therapeutic and prophylactic methods and compositions can be provided in a combined amount effective to achieve the desired effect, such as the killing of a cancer cell and/or the inhibition of cellular hyperproliferation. This process may involve contacting the cells with both an inhibitor of gene expression and a second therapy.
  • a tissue, tumor, or cell can be contacted with one or more compositions or pharmacological formulation(s) including one or more of the agents (i.e., inhibitor of gene expression or an anti-cancer agent), or by contacting the tissue, tumor, and/or cell with two or more distinct compositions or formulations, wherein one composition provides 1) an inhibitor of gene expression; 2) an anti-cancer agent, or 3) both an inhibitor of gene expression and an anti- cancer agent.
  • a combination therapy can be used in conjunction with a chemotherapy, radiotherapy, surgical therapy, or immunotherapy.
  • An inhibitor of gene expression may be administered before, during, after or in various combinations relative to an anti-cancer treatment.
  • the administrations may be in intervals ranging from concurrently to minutes to days to weeks.
  • the inhibitor of gene expression is provided to a patient separately from an anti-cancer agent, one would generally ensure that a significant period of time did not expire between the time of each delivery, such that the two compounds would still be able to exert an advantageously combined effect on the patient.
  • a course of treatment will last 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90 days or more.
  • one agent may be given on day 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, and/or 90, any combination thereof, and another agent is given on day 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45,
  • the patient may be given one or multiple administrations of the agent(s). Moreover, after a course of treatment, it is contemplated that there is a period of time at which no anti-cancer treatment is administered. This time period may last 1, 2, 3, 4, 5, 6, 7 days, and/or 1, 2, 3, 4, 5 weeks, and/or 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12 months or more, depending on the condition of the patient, such as their prognosis, strength, health, etc. [000133] Various combinations may be employed. For the example below an inhibitor of gene expression therapy is "A" and an anti-cancer therapy is "B":
  • Administration of any compound or therapy of the present invention to a patient will follow general protocols for the administration of such compounds, taking into account the toxicity, if any, of the agents. Therefore, in some embodiments there is a step of monitoring toxicity that is attributable to combination therapy. It is expected that the treatment cycles would be repeated as necessary. It also is contemplated that various standard therapies, as well as surgical intervention, may be applied in combination with the described therapy.
  • a standard therapy will include chemotherapy, radiotherapy, immunotherapy, surgical therapy or gene therapy and may be employed in combination with the inhibitor of gene expression therapy, anticancer therapy, or both the inhibitor of gene expression therapy and the anti-cancer therapy, as described herein. 1.
  • chemotherapeutic agents may be used in accordance with the present invention.
  • the term "chemotherapy” refers to the use of drugs to treat cancer.
  • chemotherapeutic agent is used to connote a compound or composition that is administered in the treatment of cancer. These agents or drugs are categorized by their mode of activity within a cell, for example, whether and at what stage they affect the cell cycle. Alternatively, an agent may be characterized based on its ability to directly cross-link DNA, to intercalate into DNA, or to induce chromosomal and mitotic aberrations by affecting nucleic acid synthesis. Most chemotherapeutic agents fall into the following categories: alkylating agents, antimetabolites, antitumor antibiotics, mitotic inhibitors, and nitrosoureas.
  • the chemotherapeutic agent is a taxane, a taxane derivative, a taxane metabolite, or a taxane prodrug.
  • taxanes include docetaxel, paclitaxel, abraxane, 7-epipaclitaxel, t-acetyl paclitaxel, 10-desacetyl-paclitaxel, 10- desacetyl-7-epipaclitaxel, 7-xylosylpaclitaxel, lO-desacetyl-7-glutarylpaclitaxel, 7-N 5 N- dimethylglycylpaclitaxel, 7-L-alanylpaclitaxel, or a mixture thereof.
  • chemotherapeutic agents include alkylating agents such as thiotepa and cyclosphosphamide; alkyl sulfonates such as busulfan, improsulfan and piposulfan; aziridines such as benzodopa, carboquone, meturedopa, and uredopa; ethylenimines and methylamelamines including altretamine, triethylenemelamine, trietylenephosphoramide, triethiylenethiophosphoramide and trimethylolomelamine; acetogenins (especially bullatacin and bullatacinone); a camptothecin (including the synthetic analogue topotecan); bryostatin; callystatin; CC- 1065 (including its adozelesin, carzelesin and bizelesin synthetic analogues); cryptophycins (particularly cryptophycin 1 and cryptophycin 8); dolastatin; du
  • anti-hormonal agents that act to regulate or inhibit hormone action on tumors
  • SERMs selective estrogen receptor modulators
  • aromatase inhibitors that inhibit the enzyme aromatase, which regulates estrogen production in the adrenal glands, such as, for example, 4(5)-imidazoles, aminoglutethimide, megestrol acetate, exemestane, formestanie, fadrozole, vorozole, letrozole, and anastrozole
  • anti-androgens such as flutamide, nilutamide, bicalutamide, leuprolide, and goserelin; as well as troxacitabine
  • DNA damaging factors include what are commonly known as ⁇ -rays, X-rays, and/or the directed delivery of radioisotopes to tumor cells.
  • Other forms of DNA damaging factors are also contemplated such as microwaves, proton beam irradiation (U.S. Patents 5,760,395 and 4,870,287) and UV- irradiation. It is most likely that all of these factors affect a broad range of damage on DNA, on the precursors of DNA, on the replication and repair of DNA, and on the assembly and maintenance of chromosomes.
  • Dosage ranges for X-rays range from daily doses of 50 to 200 roentgens for prolonged periods of time (3 to 4 wk), to single doses of 2000 to 6000 roentgens.
  • Dosage ranges for radioisotopes vary widely, and depend on the half-life of the isotope, the strength and type of radiation emitted, and the uptake by the neoplastic cells.
  • the terms "contacted" and "exposed,” when applied to a cell, are used herein to describe the process by which a therapeutic construct and a chemotherapeutic or radiotherapeutic agent are delivered to a target cell or are placed in direct juxtaposition with the target cell. To achieve cell killing, for example, both agents are delivered to a cell in a combined amount effective to kill the cell or prevent it from dividing.
  • immunotherapeutics In the context of cancer treatment, immunotherapeutics, generally, rely on the use of immune effector cells and molecules to target and destroy cancer cells.
  • Trastuzumab (HerceptinTM) is such an example.
  • the immune effector may be, for example, an antibody specific for some marker on the surface of a tumor cell.
  • the antibody alone may serve as an effector of therapy or it may recruit other cells to actually affect cell killing.
  • the antibody also may be conjugated to a drug or toxin (chemotherapeutic, radionuclide, ricin A chain, cholera toxin, pertussis toxin, etc.) and serve merely as a targeting agent.
  • the effector may be a lymphocyte carrying a surface molecule that interacts, either directly or indirectly, with a tumor cell target.
  • Various effector cells include cytotoxic T cells and NK cells. The combination of therapeutic modalities, i.e., direct cytotoxic activity and inhibition or reduction of ErbB2 would provide therapeutic benefit in the treatment of ErbB2 overexpressing cancers.
  • Another immunotherapy could also be used as part of a combined therapy with gen silencing therapy discussed above.
  • the tumor cell must bear some marker that is amenable to targeting, i.e., is not present on the majority of other cells.
  • tumor markers exist and any of these may be suitable for targeting in the context of the present invention.
  • Common tumor markers include carcinoembryonic antigen, prostate specific antigen, urinary tumor associated antigen, fetal antigen, tyrosinase ( ⁇ 97), gp68, TAG-72, HMFG, Sialyl Lewis Antigen, MucA, MucB, PLAP, estrogen receptor, laminin receptor, erb B and pi 55.
  • An alternative aspect of immunotherapy is to combine anticancer effects with immune stimulatory effects.
  • Immune stimulating molecules also exist including: cytokines such as IL-2, IL-4, IL- 12, GM-CSF, gamma-IFN, chemokines such as MIP-I,
  • immune stimulating molecules either as proteins or using gene delivery in combination with a tumor suppressor has been shown to enhance anti-tumor effects (Ju et al. , 2000). Moreover, antibodies against any of these compounds can be used to target the anti-cancer agents discussed herein.
  • immunotherapies currently under investigation or in use are immune adjuvants e.g., Mycobacterium bovis, Plasmodium falciparum, dinitrochlorobenzene and aromatic compounds (U.S.
  • Patents 5,830,880 and 5,846,945) and monoclonal antibodies e.g., anti-ganglioside GM2, anti-HER- 2, anti-pl85 (Pietras et al, 1998; Hanibuchi et al, 1998; U.S. Patent 5,824,311). It is contemplated that one or more anti-cancer therapies may be employed with the gene silencing therapies described herein.
  • an antigenic peptide, polypeptide or protein, or an autologous or allogenic tumor cell composition or "vaccine” is administered, generally with a distinct bacterial adjuvant (Ravindranath and Morton, 1991; Morton et al, 1992; Mitchell et al, 1990; Mitchell et al, 1993).
  • adoptive immunotherapy the patient's circulating lymphocytes, or tumor infiltrated lymphocytes, are isolated in vitro, activated by lymphokines such as IL-2 or transduced with genes for tumor necrosis, and readministered (Rosenberg et al, 1988; 1989).
  • Curative surgery is a cancer treatment that may be used in conjunction with other therapies, such as the treatment of the present invention, chemotherapy, radiotherapy, hormonal therapy, gene therapy, immunotherapy and/or alternative therapies.
  • Curative surgery includes resection in which all or part of cancerous tissue is physically removed, excised, and/or destroyed.
  • Tumor resection refers to physical removal of at least part of a tumor.
  • treatment by surgery includes laser surgery, cryosurgery, electrosurgery, and microscopically controlled surgery (Mohs' surgery). It is further contemplated that the present invention may be used in conjunction with removal of superficial cancers, precancers, or incidental amounts of normal tissue.
  • a cavity may be formed in the body. Treatment may be accomplished by perfusion, direct injection or local application of the area with an additional anti-cancer therapy. Such treatment may be repeated, for example, every 1, 2, 3, 4, 5, 6, or 7 days, or every 1, 2, 3, 4, and 5 weeks or every 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, or 12 months. These treatments may be of varying dosages as well.
  • agents may be used in combination with the present invention to improve the therapeutic efficacy of treatment.
  • additional agents include immunomodulatory agents, agents that affect the upregulation of cell surface receptors and GAP junctions, cytostatic and differentiation agents, inhibitors of cell adhesion, agents that increase the sensitivity of the hyperproliferative cells to apoptotic inducers, or other biological agents.
  • Immunomodulatory agents include tumor necrosis factor; interferon alpha, beta, and gamma; IL-2 and other cytokines; F42K and other cytokine analogs; or MIP-I, MIP-I beta, MCP-I, RANTES, and other chemokines.
  • cytostatic or differentiation agents can be used in combination with the present invention to improve the anti-hyerproliferative efficacy of the treatments.
  • Inhibitors of cell adhesion are contemplated to improve the efficacy of the present invention.
  • Examples of cell adhesion inhibitors are focal adhesion kinase (FAKs) inhibitors and Lovastatin. It is further contemplated that other agents that increase the sensitivity of a hyperproliferative cell to apoptosis, such as the antibody c225, could be used in combination with the present invention to improve the treatment efficacy.
  • FAKs focal adhesion kinase
  • Lovastatin agents that increase the sensitivity of a hyperproliferative cell to apoptosis
  • the antibody c225 could be used in combination with the present invention to improve the treatment efficacy.
  • hyperthermia is a procedure in which a patient's tissue is exposed to high temperatures (up to 106°F).
  • External or internal heating devices may be involved in the application of local, regional, or whole-body hyperthermia.
  • Local hyperthermia involves the application of heat to a small area, such as a tumor. Heat may be generated externally with high-frequency waves targeting a tumor from a device outside the body. Internal heat may involve a sterile probe , including thin, heated wires or hollow tubes filled with warm water, implanted microwave antennae, or radiofrequency electrodes.
  • a patient's organ or a limb is heated for regional therapy, which is accomplished using devices that produce high energy, such as magnets.
  • some of the patient's blood may be removed and heated before being perfused into an area that will be internally heated.
  • Whole-body heating may also be implemented in cases where cancer has spread throughout the body. Warm-water blankets, hot wax, inductive coils, and thermal chambers may be used for this purpose.
  • Hormonal therapy may also be used in conjunction with the present invention or in combination with any other cancer therapy previously described.
  • the use of hormones may be employed in the treatment of certain cancers such as breast, prostate, ovarian, or cervical cancer to lower the level or block the effects of certain hormones such as testosterone or estrogen. This treatment is often used in combination with at least one other cancer therapy as a treatment option or to reduce the risk of metastases.
  • H. Kits and Diagnostics
  • kits are envisioned containing therapeutic agents and/or other therapeutic and delivery agents.
  • the present invention contemplates a kit for preparing and/or administering a therapy of the invention.
  • the kit may comprise one or more sealed vials containing any of the pharmaceutical compositions of the present invention.
  • the lipid is in one vial, and the nucleic acid component is in a separate vial.
  • the kit may include may include at least one inhibitor of STAT expression, one or more lipid component, as well as reagents to prepare, formulate, and/or administer the components of the invention or perform one or more steps of the inventive methods.
  • the kit may also comprise a suitable container means, which is a container that will not react with components of the kit, such as an eppendorf tube, an assay plate, a syringe, a bottle, or a tube.
  • the container may be made from sterilizable materials such as plastic or glass.
  • the kit may further include an instruction sheet that outlines the procedural steps of the methods, and will follow substantially the same procedures as described herein or are known to those of ordinary skill.
  • the instruction information may be in a computer readable media containing machine-readable instructions that, when executed using a computer, cause the display of a real or virtual procedure of delivering a pharmaceutically effective amount of a composition of the present invention to a subject in need.
  • the SKOV3 cell line (Buick et al, 1985) was obtained from American Type Tissue Culture and maintained in Minimal Essential Media
  • EG cells (Stalling et al, 1996) were maintained in RPMI-1640 with 15% FBS and 0.1% MITO Plus reagent (BD Biosciences,
  • Norepinephrine, epinephrine, and propanolol were purchased from Sigma (St. Louis, MO) and reconstituted and stored in 5N
  • KT5720 (Calbiochem, San Diego, CA) were stored at -20 0 C.
  • Anti-IL-6 antibody was from (Biosource, Camarillo, CA). When used, these inhibitory substances were exposed to cells for 1 hour prior to exposure to norepinephrine or epinephrine.
  • Western Blot Cell lysate was prepared by washing cells with PBS and incubated for 10 min at 4 0 C in modified RIPA lysis buffer (5OmM Tris, 15OmM NaCI, 1% triton, 0.5% deoxycholate plus the protease inhibitors leupeptin, aprotinin, EDTA, and sodium vanadate (Sigma).
  • DNA-binding activity of STAT3 was assessed by electrophoretic mobility shift assay (EMSA) of nuclear extracts from 10 7 cultured cells, serum starved for 15 hours and either untreated or treated with NE for 5, 15, 30, and 60 minutes.
  • ESA electrophoretic mobility shift assay
  • Nuclear extracts were obtained by differential lysis at 4°C (Read, 1996), and 1/25 (2 ⁇ l) of the resulting extract was incubated at room temperature for 15 min with 1.75 pmol of 32 P-labeled STAT3 consensus oligonucleotide (Promega, Madison, WI) in a 10 aqueous binding reaction containing 2 ⁇ l of 5x gel shift binding buffer (20% glycerol, 5mM MgC12, 2.5mM EDTA, 2.5mM DTT, 25OmM NaCI, 5OmM Tris-HCI, pH 7.5, and. 25 mg/ml poly(dl-dC); Promega).
  • 5x gel shift binding buffer 20% glycerol, 5mM MgC12, 2.5mM EDTA, 2.5mM DTT, 25OmM NaCI, 5OmM Tris-HCI, pH 7.5, and. 25 mg/ml poly(dl-dC); Promega).
  • Bound oligonucleotides were resolved on a 6% polyacrylamide gel (run for 90 min at 250V following a 15 min pre-run) and quantified on a Storm 860 phosphoimager using ImageQuant software (Molecular Dynamics, Sunnyvale, CA). All binding reactions were oligonucleotide-specific as demonstrated by competitive inhibition when protein extracts were preincubated with 100-fold excess of unlabeled target oligonucleotide, but not when extracts were preincubated with similar concentrations of an unlabeled control oligonucleotide. Equivalent loading of nuclear protein into each binding reaction was verified by Bradford assay (Bio-Rad).
  • siRNA Small interfering RNA.
  • STAT3 -specific siRNA was purchased from Qiagen (Valencia, CA),
  • RNAiFect transfection agent (1 ⁇ g siRNA to 6 ⁇ g RNAiFect) and exposed to cells at 70-80% confluence for 24 hours prior to use in the MICS invasion system or collection of supernatant for assessment of MMP production.
  • siRNA was encapsulated into the neutral liposome, 1 ,2-Dioleoyl-sn-Glycero-3 -Phosphatidylcholine (DOPC) as previously described (Landen et al, 2005). For each treatment, 3.5 ⁇ g of siRNA was reconstituted in the neutral liposome, 1 ,2-Dioleoyl-sn-Glycero-3 -Phosphatidylcholine (DOPC) as previously described (Landen et al, 2005). For each treatment, 3.5 ⁇ g of siRNA was reconstituted in
  • the Membrane Invasion Culture System (MICS) chamber was used to measure the in vitro invasiveness of all cell lines used in this study (Chambers, 2000; Sood et al, 2001; Sood et al, 2004). SiRNA was exposed to cells 24 hours prior to cell harvest, and 10 ⁇ M of NE was exposed for 3 hours prior to harvest and testing for invasion.
  • MICS assay a polycarbonate membrane with 10 ⁇ iM pores (Osmonics; Livermore, CA) was uniformly coated with a defined basement membrane matrix consisting of human laminin/type IV collagen/gelatin and used as the intervening barrier to invasion.
  • the defined matrix was prepared (stored at 4°C) in a 10 mL stock solution as follows: laminin (50 ⁇ g/mL) ImL + type IV collagen (50 ⁇ g/mL) 0.2 mL + gelatin (2 mg/mL) 4mL + 4.8 mL PBS. Using a disposable pipet, 1 mL of the matrix solution was dispensed across a long side of the membrane. An 8 mm glass rod was used to spread the matrix across the membrane, and allowed to dry for 30 minutes. The matrix coated filter was placed coated side up on the lower plate followed by carefully attaching the upper plate. Both upper and lower wells of the chamber were filled with serum-free RPMI containing IX MITO+(Collaborative Biomedical; Bedford, MA).
  • Single cell tumor suspensions were seeded into the upper wells at a concentration of 1 X 10 cells per well. Following a 24 hour incubation in a humidified incubator at 37°C with 5% CO 2 , cells that had invaded through the basement membrane were collected through the sideport by replacing the media in the lower chamber with 2mM EDTA/PBS, pH 7.4, for 20 minutes at 37°C. The cells recovered from the bottom of the filter were then loaded onto a dot blot manifold containing 3 ⁇ m pore polycarbonate filters, fixed, stained, and counted by light microscopy (Sood et al, 2001; Sood et al, 2004). Invasiveness was calculated as the percentage of cells that had successfully invaded through the matrix-coated membrane to the lower wells relative to the total number of cells seeded into the upper wells. The invasion assays were performed in triplicate and repeated once.
  • MMP matrix metalloproteinase
  • the protein concentration of total MMP-2 (pro-and active MMP-2), and total MMP-9 (92kDa pro- and 82kDa active forms) were determined using Quantikine immunoassays (R&D Systems; Minneapolis, MN) as per the manufacturer's protocols.
  • the concentrations of active MMP-2 and MMP-9 were determined using the Biotrak Activity Assay System (Amersham Biosciences, Piscataway, NJ) as per the manufacturer's protocols.
  • the MMP experiments were performed in triplicate and repeated once. [000165] In vivo tumor model. Female nude mice were purchased from the National Cancer Institute - Frederick Cancer Research Facility (Frederick, MD).
  • mice were housed and maintained under specific pathogen-free conditions in facilities approved by the American Association for Accreditation of Laboratory Animal Care in accordance with current regulations and standards of the United States Department of Agriculture, United States Department of Health and Human Services, and the National Institutes of Health. The mice were used according to institutional guidelines when they were 8-12 weeks of age.
  • SKOV3ipl tumor cells were harvested from subconfluent cultures by a brief exposure to 0.25% trypsin and 0.02% EDTA. Trypsinization was stopped with medium containing 10% FBS. The cells were then washed once in serum-free medium and resuspended in Hank's Balanced salt solution (serum free).
  • mice were treated with daily injections of PBS (200 ⁇ l, intraperitoneal (IP)), isoproterenol (10 mg/kg daily IP), or isoproterenol (10 mg/kg) in combination with siRNA (control or STAT3 -specific, 3.5 ⁇ g in DOPC every 3 days, IP) for 7 days (Landen et ah, 2006).
  • PBS 200 ⁇ l, intraperitoneal (IP)
  • isoproterenol 10 mg/kg daily IP
  • siRNA control or STAT3 -specific, 3.5 ⁇ g in DOPC every 3 days, IP
  • mice All treatments were administered in a total volume of 200 ⁇ l.
  • Norepinephrine induces translocation of STAT3 to the nucleus. Studies were then conducted to confirm that once STAT3 was activated, it was directed to the nucleus. Staining of SKOV3 cells in culture chambers after norepinephrine exposure showed that compared to untreated cells (FIG. 2A), increased levels of activated STAT3 were seen in as little as 15 minutes (FIG. 2C). This expression induction was predominantly in the nucleus. Pretreatment with propanolol again prevented the norepinephrine-induced effects on STAT3 (FIG. 2D), suggesting that STAT3 activation is mediated via ⁇ -adrenergic receptors.
  • Neuroendocrine hormones bind adrenergic receptors and activate the G-protein adenylyl cyclase, which activates the second messenger molecule cyclic AMP.
  • Multiple pathways are activated by cyclic AMP, including that mediated by protein kinase A.
  • cells were preincubated with the protein kinase A inhibitor KT5720 for one hour prior to norepinephrine exposure.
  • the MICS chamber system was used to quantify cell invasion with NE with or without STAT3 downregulation (FIG. 5B).
  • the STAT3-siRNA completely blocked the norepinephrine-mediated increase in invasion, whereas the control siRNA had no effect.
  • studies were conducted to investigate whether the invasion rates could be affected by changes in proliferation.
  • the effects of norepinephrine and epinephrine on ovarian cancer cell proliferation was studied using the MTT assay. Neither of these two catecholamines affected cell proliferation despite testing multiple doses and time periods.
  • STAT3 siRNA-DOPC reduced tumor growth by 47.1 (p ⁇ 0.01) under basal conditions.
  • STAT3 siRNA-DOPC completely blocked isoproterenolstimulated tumor growth, reducing tumor volume by 85% and infiltration.

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  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

La présente invention concerne les domaines de la biologie moléculaire et de la délivrance de médicaments. Sont décrites des compositions qui comprennent un acide nucléique ciblé sur un gène STAT et un lipide neutre. Sont également décrits des procédés de traitement d'un sujet humain ayant une maladie associée à l'expression accrue d'un STAT impliquant l'administration au sujet d'une quantité pharmaceutiquement efficace d'une composition qui comprend un acide nucléique ciblé sur un gène STAT et un lipide neutre. Des modes de réalisation spécifiques se rapportent à un procédé de traitement d'un cancer comprenant l'administration à un sujet d'une quantité pharmaceutiquement efficace d'une composition qui comprend un ARNsi ciblé sur STAT3 et un lipide neutre.
PCT/US2008/066753 2007-06-22 2008-06-12 Acide nucléique inhibiteur des liposomes contre les protéines stat WO2009002719A1 (fr)

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US8900627B2 (en) 2008-06-06 2014-12-02 Mirna Therapeutics, Inc. Compositions for the in vivo delivery of RNAi agents
GB2515658A (en) * 2013-06-27 2014-12-31 Pgs Geophysical As Survey techniques using streamers at different depths
FR3038606A1 (fr) * 2015-07-06 2017-01-13 Inst Nat Sciences Appliquees Lyon Glycerophosphocholines, pour leur utilisation dans le traitement d'une inflammation intestinale ou d'un cancer intestinal
WO2019027055A1 (fr) * 2017-08-04 2019-02-07 協和発酵キリン株式会社 Nanoparticules lipidiques contenant un acide nucléique
US11092710B2 (en) 2013-06-27 2021-08-17 Pgs Geophysical As Inversion techniques using streamers at different depths
CN115944590A (zh) * 2023-02-02 2023-04-11 上海交通大学 一种基因/药物共载脂质体及其制备方法与用途

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Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8900627B2 (en) 2008-06-06 2014-12-02 Mirna Therapeutics, Inc. Compositions for the in vivo delivery of RNAi agents
GB2515658A (en) * 2013-06-27 2014-12-31 Pgs Geophysical As Survey techniques using streamers at different depths
GB2515658B (en) * 2013-06-27 2020-07-29 Pgs Geophysical As Survey techniques using streamers at different depths
US11092710B2 (en) 2013-06-27 2021-08-17 Pgs Geophysical As Inversion techniques using streamers at different depths
FR3038606A1 (fr) * 2015-07-06 2017-01-13 Inst Nat Sciences Appliquees Lyon Glycerophosphocholines, pour leur utilisation dans le traitement d'une inflammation intestinale ou d'un cancer intestinal
WO2019027055A1 (fr) * 2017-08-04 2019-02-07 協和発酵キリン株式会社 Nanoparticules lipidiques contenant un acide nucléique
JPWO2019027055A1 (ja) * 2017-08-04 2020-08-20 協和キリン株式会社 核酸含有脂質ナノ粒子
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CN115944590A (zh) * 2023-02-02 2023-04-11 上海交通大学 一种基因/药物共载脂质体及其制备方法与用途

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