WO2009093367A1

WO2009093367A1 - Ethanol production process

Info

Publication number: WO2009093367A1
Application number: PCT/JP2008/069523
Authority: WO
Inventors: Mikio Kuzuu
Original assignee: Mikio Kuzuu
Priority date: 2008-01-21
Filing date: 2008-10-28
Publication date: 2009-07-30

Abstract

[PROBLEMS] To provide an ethanol production process which is easy to operate and has excellent production efficiency. [MEANS FOR SOLVING PROBLEMS] Disclosed is an ethanol production process comprising: a production step of irradiating a mixture of a sugar-containing solid-liquid mixture, an yeast cell and a plant chloroplast with a light emitted from a light-emitting diode in an enclosed system to produce ethanol; and a collection step of collecting ethanol thus produced from the system to the outside of the system, wherein the following reactions a) to c) proceed concurrently in the production step: a) a reaction for fermenting the sugar-containing solid-liquid mixture with the yeast cell to produce ethanol; b) a reaction for photosynthesizing carbon dioxide that is produced during the fermentation reaction a) with the plant chloroplast by irradiating with a light emitted from the light-emitting diode to produce a sugar; and c) a reaction for fermenting the sugar produced in the reaction b) with the yeast cell to produce ethanol.

Description

Ethanol production method

The present invention is a reaction in which a solid-liquid mixture containing sugar is fermented with yeast to produce ethanol, and carbon dioxide generated during the fermentation is photo-synthesized by light irradiation of a plant chloroplast and a light-emitting diode to produce sugar. The reaction and the reaction of fermenting the sugar obtained in the reaction with yeast to produce ethanol proceed in parallel in the same container. The present invention relates to a method for producing ethanol.

Japanese Patent Application Laid-Open No. 07-087986 discloses “microalgae culture means 1” for culturing microalgae that accumulate starch in cells by photosynthesis, and “microalgae concentration” for concentrating a culture solution containing algal bodies cultured by this means. A method for producing ethanol comprising means 2 ”and“ holding means 3 ”for producing ethanol by maintaining the slurry obtained by the means in a dark and anaerobic atmosphere while maintaining the pH in the range of 6.0 to 9.0 is described. Has been.
In the above production method, “microalgae culture means 1” and “holding means 3” are completely independent separate processes, and a separate process of “microalgae concentration means 2” is required between the two processes. Therefore, the operation is complicated, and it cannot be said that the ethanol production method is always highly efficient.
In particular, since the “holding means 3” for generating ethanol needs to be kept in a dark and anaerobic atmosphere, it can be performed in parallel with the “microalgae culture means 1” that requires light irradiation in the same container. It was impossible.

Japanese Patent Laid-Open No. 2007-325564 discloses a method for inoculating koji mold and saccharifying a raw material subjected to a specific treatment to produce ethanol using rice as a cereal as a starting material and adding yeast to perform ethanol fermentation. In addition, a method of synthesizing and utilizing industrial methanol or the like by simply reusing carbon dioxide generated as a by-product during ethanol fermentation without letting it escape to the atmosphere is described.
Although the above method describes the reuse of carbon dioxide generated during ethanol fermentation, the carbon dioxide is converted into a sugar that is a raw material for ethanol fermentation by photosynthesis, and the sugar is converted into the above ethanol fermentation. There was no suggestion or description about performing in parallel in the same container.

In addition, a method for producing various organic compounds by using a light-emitting diode as a light source for photosynthesis to produce useful organic substances has been reported (for example, Japanese Patent Application Laid-Open Nos. 10-113164 and 2004-147461). , JP 2002-315569, Registered Utility Model No. 3073908, Tomonao Katsuta, Biochemical Engineering Study for Production of Useful Substances by Photobioreactor, 59th Annual Meeting of the Japanese Society for Biotechnology August 2, 2007, p. 107 (Lecture number: 3D09-1)).
However, none of the above methods describe a specific method for producing sugar from carbon dioxide by photosynthesis.
Japanese Patent Laid-Open No. 07-087986 JP 2007-325564 A JP-A-10-113164 JP 2004-147461 A JP 2002-315569 A Registered Utility Model No. 3073908 Katsuta Tomonao, Biochemical engineering study for production of useful substances by photobioreactor, Abstracts of the 59th Annual Meeting of the Japanese Society for Biotechnology, August 2, 2007, p. 107 (lecture number: 3D09-1)

The present invention is a reaction in which a solid-liquid mixture containing sugar is fermented with yeast to produce ethanol, and carbon dioxide generated during the fermentation is photo-synthesized by light irradiation of a plant chloroplast and a light-emitting diode to produce sugar. The reaction and the reaction of fermenting the sugar obtained in the reaction with yeast to produce ethanol proceed in parallel in the same container. An object is to provide a method for producing ethanol.

As a result of intensive studies to solve the above problems, the present inventors have performed a simple operation of irradiating light from a light-emitting diode in a closed system containing a solid-liquid mixture containing sugar, yeast and plant chloroplasts. By
a) a reaction in which a solid-liquid mixture containing sugar is fermented with yeast to produce ethanol;
b) Reaction of photosynthesis of carbon dioxide generated during fermentation in a) by light irradiation of plant chloroplasts and light emitting diodes to sugar, and c) fermentation of sugar obtained in b) with yeast. The present inventors have found that the reaction for producing ethanol proceeds in parallel.
Moreover, it discovered that ethanol was efficiently manufactured by irradiating the light of a specific wavelength range in this case.

That is, the present invention
(1) A production process for producing ethanol by irradiating light from a light-emitting diode in a sealed system containing a solid-liquid mixture containing sugar, yeast and plant chloroplasts, and ethanol produced from the system. A method for producing ethanol comprising a recovery step of recovering outside,
In the generating step,
a) a reaction in which a solid-liquid mixture containing sugar is fermented with yeast to produce ethanol;
b) Reaction of photosynthesis of carbon dioxide generated during fermentation in a) by light irradiation of plant chloroplasts and light emitting diodes to sugar, and c) fermentation of sugar obtained in b) with yeast. The reaction for producing ethanol proceeds in parallel, and the solid-liquid mixture containing sugar is saccharified by pyrolysis or hydrolysis of cellulose or hemicellulose, or rice, wheat, straw or corn containing starch Saccharify with koji, malt or enzyme, crush vegetables and / or fruits, or crush vegetables and / or fruits, then heat concentration method, freeze concentration method, reverse osmosis The plant chloroplast is prepared by concentrating by one or more concentration methods selected from a concentration method and a vacuum concentration method, and the plant chloroplast is a seed plant, a fern plant, an algae, a moss plant, a bacterium Or a mixture of these The method of producing ethanol is a plant chloroplast things,
(2) The light of the light emitting diode is light whose total light emission amount in the wavelength regions of 380 to 520 nm and 620 to 780 nm is 70% or more of the light emission amount over the entire wavelength region. Production method,
(3) The method for producing ethanol according to (1) or (2), wherein further carbon dioxide is added to the sealed system,
(4) The method for producing ethanol according to any one of (1) to (3), wherein the recovery step is by distillation,
About.

“The total light emission amount in the wavelength range of 380 to 520 nm and 620 to 780 nm is 70% or more of the light emission amount over the entire wavelength region” means that the light emission spectrum of the light emitting diode used is measured and recorded. It means that a value indicating the sum of the spectrum areas in the wavelength regions of 380 to 520 nm and 620 to 780 nm with respect to the total spectrum area as a percentage is 70% or more.

The ethanol production method of the present invention produces sugar by a photosynthesis reaction using carbon dioxide generated in a sugar fermentation reaction as a raw material, and therefore does not discharge carbon dioxide. It can be said that this is a manufacturing method.
In addition, since ethanol is produced using sugar produced by the photosynthesis reaction as a raw material, the ethanol recovery rate is high compared to normal ethanol fermentation, and three reactions are simultaneously performed in one container. Since it can be performed, it is a very excellent ethanol production method that is simple and efficient in operation.
In a preferred embodiment of the present invention, additional carbon dioxide is added into the reaction system, whereby ethanol can be produced more efficiently.

The method for producing ethanol of the present invention comprises a production step of producing ethanol by irradiating light from a light-emitting diode in a sealed system containing a solid-liquid mixture containing sugar, yeast and plant chloroplasts, and from the system. A recovery step of recovering the produced ethanol out of the system.

The solid-liquid mixture containing sugar used in the present invention is saccharified by pyrolyzing or hydrolyzing cellulose or hemicellulose, or rice, wheat, straw or corn containing starch is saccharified using straw, malt or an enzyme agent. Or after pulverizing vegetables and / or fruits, or after pulverizing vegetables and / or fruits, one kind selected from a heat concentration method, a freeze concentration method, a reverse osmosis concentration method and a vacuum concentration method or It is prepared by concentrating by two or more kinds of concentration methods.
Examples of cellulose or hemicellulose include cellulose or hemicellulose obtained from a plant body such as wood.
Saccharification of cellulose by thermal decomposition can be performed by a known method, for example, a method described in Japanese Patent No. 1400009.
The saccharification of cellulose or hemicellulose by hydrolysis can be carried out by a known method, for example, a method of hydrolysis using dilute sulfuric acid described in JP-B 61-0444479.
In addition, the solid-liquid mixture containing sugar is made of rice, wheat, straw or corn containing starch, and if necessary, subjected to a step of removing the hull, pulverization or pulverization, and the like. It can be prepared by adding.
Examples of the enzyme agent include amylase.
In addition, a solid-liquid mixture containing sugar is obtained by crushing vegetables and / or fruits, or crushing vegetables and / or fruits, followed by a heat concentration method, a freeze concentration method, a reverse osmosis concentration method, and a vacuum. It can prepare by concentrating by the 1 type (s) or 2 or more types of concentration method chosen from the concentration method.
The vegetables are not limited as long as they contain sugar, but specific examples include radish, sugar beet, cabbage, lettuce, Chinese cabbage, sugar cane, tomato, carrot, onion, leek and the like.
Fruits are not limited as long as they contain sugar, but specific examples include apples, tangerines, grapes, white peaches, yellow peaches, pears, pineapples, mangoes, bananas, melons, cherries, loquats, and blueberries. , Strawberry, kiwi, raspberry, blackberry, apricot, oyster, watermelon and the like.
The vegetables and fruits are preferably washed before pulverization.
As a pulverization method, a method usually used for pulverizing vegetables and / or fruits can be used, and examples thereof include pulverization using a mixer (for home use and business use).
The solid-liquid mixture containing sugar prepared by pulverizing vegetables and / or fruits can be further concentrated.
Concentration is particularly effective when the sugar content in the solid-liquid mixture is low.
Examples of the concentration method include a heat concentration method, a freezing concentration method, a reverse osmosis concentration method, a vacuum concentration method, and a concentration method in which two or more of these are performed simultaneously or continuously.
The above-mentioned concentration methods (heat concentration method, freeze concentration method, reverse osmosis concentration method, vacuum concentration method) are usually methods known in the technical field (heat concentration method, freeze concentration method, reverse osmosis concentration method, vacuum concentration method). Can be done.
In addition, the solid-liquid mixture containing the saccharide prepared above can be sterilized as necessary.

The yeast used in the present invention is not particularly limited as long as it can produce ethanol, and specific examples include wine yeast, sake yeast, whiskey yeast, beer yeast and the like.

Plant chloroplasts used in the present invention include plant chloroplasts of seed plants, ferns, algae, moss plants, bacteria, or mixtures thereof.
Although it does not specifically limit as a seed plant, For example, a ginkgo, rice, etc. are preferable.
Although it does not specifically limit as a fern plant, For example, a cycad etc. are preferable.
Although it does not specifically limit as algae, For example, kawamozuku, gray algae, crypt algae, wakame, euglena algae, chloracunion algae, cyanobacteria, uremo etc. are preferable.
Although it does not specifically limit as a moss plant, For example, a sphagnum moss, a black moss, a scallop, a scale moss, a sphagnum, a hornbill, etc. are preferable.
Although it does not specifically limit as bacteria, For example, cyanobacteria (extracted from a sea lion) etc. are preferable.
Preferred plant chloroplasts include bacterial plant chloroplasts and cyanobacterial plant chloroplasts.

In general, the plant chloroplast used in the present invention preferably destroys the plant cell membrane so that the sugar produced by photosynthesis is efficiently fermented by the yeast in the solution. The destruction of the cell membrane of the plant can be performed, for example, by crushing the plant.
However, if the produced sugar is efficiently fermented without destroying the cell membrane, it is not necessary to destroy the cell membrane.

The sealed system is not particularly limited as long as it is a sealed container that can be irradiated with light and can cope with a volume change caused by the generated gas (carbon dioxide, oxygen). A container etc. are mentioned.
In the case of a glass container, light irradiation can be performed from the outside and / or the inside of the glass container, and in the case of a metal container, light irradiation can be performed from the inside of the container.
Moreover, it is preferable that the said container is equipped with the expansion-contraction site | part which can accommodate the produced | generated gas temporarily so that it can respond to the produced | generated gas.
The system is preferably stirrable.

In addition, the glass container is, for example, a glass tube installed around a light emitting diode, and a solid-liquid mixture containing sugar, a solution containing yeast and plant chloroplasts are allowed to flow at a rate capable of an ethanol production reaction. It can also be a container capable of continuous reaction.
The glass container is composed of, for example, two glass plates capable of irradiating light from a light-emitting diode from both the front and back surfaces, a solid-liquid mixture containing sugar between the glass plates, yeast and plant chloroplasts. It may also be a container capable of continuous reaction by flowing a solution containing

In the production method of ethanol of the present invention, the production step for producing ethanol is performed by irradiating the light of the light-emitting diode into the above-described sealed system. In the production step,
a) a reaction in which a solid-liquid mixture containing sugar is fermented with yeast to produce ethanol;
b) Reaction of photosynthesis of carbon dioxide generated during fermentation in a) by light irradiation of plant chloroplasts and light emitting diodes to sugar, and c) fermentation of sugar obtained in b) with yeast. Three reactions of producing ethanol proceed in parallel in the closed system.

Ethanol and carbon dioxide are produced by the fermentation of yeast in a) above.
The produced carbon dioxide is converted to sugar by photosynthesis b) of the light of the plant chloroplast and the light emitting diode, and oxygen is generated at this time.
The sugar is not particularly limited as long as it can be used for ethanol fermentation, and examples thereof include sucrose and glucose.
The sugar produced in b) is fermented by yeast (reaction c)) to produce ethanol and carbon dioxide. The produced carbon dioxide is again ethanol through the photosynthesis of b) and alcohol fermentation of c). By repeating this cycle, almost all of the carbon dioxide is finally converted into ethanol and oxygen.
Therefore, the progress of the reaction can be grasped by measuring the oxygen concentration in the gas present in the system.
The reaction is preferably carried out until the oxygen concentration in the gas present in the system reaches 90% or more, and more preferably the reaction is carried out until the concentration reaches 95% or more.

The light emitted from the light emitting diode used in the photosynthetic reaction is preferably light in which the total light emission amount in the wavelength regions of 380 to 520 nm and 620 to 780 nm is 70% or more of the light emission amount over the entire wavelength region, and more preferably , 80% or more of the light, and more preferably 90% or more of the light.
When the above% is less than 70, ethanol production efficiency tends to decrease.
Further, light having a wavelength shorter than 380 nm is not substantially contained, that is, the light emission amount is 5% or less, preferably 3% or less, and preferably 0%.
“The total light emission amount in the wavelength range of 380 to 520 nm and 620 to 780 nm is 70% or more of the light emission amount over the entire wavelength region” means that the light emission spectrum of the light emitting diode used is measured and recorded. It means that a value indicating the sum of the spectrum areas in the wavelength regions of 380 to 520 nm and 620 to 780 nm with respect to the total spectrum area as a percentage is 70% or more.

In addition to the production | generation process which produces | generates ethanol, the manufacturing method of ethanol of this invention includes the collection | recovery process which collect | recovers the produced | generated ethanol out of the system.
The recovery step is not particularly limited as long as it is a method capable of separating ethanol from the reaction system. For example, a known method such as a distillation method or a membrane separation method can be used.
Moreover, when recovering ethanol by a distillation method, the container used for the distillation operation can be used as it is, and the reaction liquid can be transferred to another container for use.
A preferred embodiment of the present invention is a method for producing ethanol in which additional carbon dioxide is added into the sealed system.
By further adding carbon dioxide, the production efficiency of ethanol can be further improved.
The additional addition of carbon dioxide is achieved, for example, by connecting a carbon dioxide introduction tube to the reaction vessel or placing it in an atmosphere of carbon dioxide.
When adding carbon dioxide, the entire system is sealed so that the carbon dioxide does not flow out.

In a preferred embodiment of the present invention, the recovery step is achieved by distillation.
As the distillation method, any of simple distillation, azeotropic distillation, azeotropic distillation with addition of benzene, and the like can be selected according to the required purity of ethanol.

Production Example 1: Production of a solid-liquid mixture containing sugar (rice) 6 g of amylase and 315 g of water were added to 330 g of rice flour purchased from a food store, stirred with a mixer, boiled and kept at 70 ° C. to 80 ° C. for 12 hours. By saccharifying the rice flour, 651 g of a solid-liquid mixture (rice) containing sugar was prepared.

Production Example 2: Production of sugar-containing solid-liquid mixture (wheat) Add 6 g of amylase and 315 g of water to 330 g of flour purchased from a food store, stir with a mixer, boil in water and keep at 70 ° C. to 80 ° C. for 12 hours. By saccharifying the wheat flour, 651 g of a solid-liquid mixture (wheat) containing sugar was prepared.

Production Example 3 Production of Solid-Liquid Mixture Containing Sugar (Corn) 6 g of amylase and 315 g of water are added to 330 g of corn flour purchased from a food store, stirred with a mixer, boiled and kept at 70 ° C. to 80 ° C. for 12 hours. By saccharifying the corn flour, 651 g of a solid-liquid mixture (corn) containing sugar was prepared.

Examples 1 to 10 and Comparative Example 1
217 g of the solid-liquid mixture (rice) containing sugar produced in Production Example 1 and 3 g of yeast were stirred with a mixer to prepare 220 g of a solid-liquid mixture (rice) containing sugar containing yeast.
The prepared solid-liquid mixture (rice) containing sugar containing yeast is divided into 11 100 mL beakers of 20 g each, and 10 types of different plant pulverized products are added to each beaker by 1 g and stirred. A 100 mL syringe, which is a manufactured container, was injected (Examples 1 to 10).
For comparison, 20 g of a solid-liquid mixture (rice) containing sugar containing yeast without adding plant pulverized material was injected into a 100 mL syringe (Comparative Example 1).
The eleven syringes prepared above are combined with 200 white light emitting diodes, 200 red light emitting diodes, 100 blue light emitting diodes and 100 green light emitting diodes. ) For 24 hours. The oxygen concentration of the gas collected from each syringe was measured with an oxygen concentration meter. Further, the emission spectrum of the light emitting diode was measured, and the ratio of the spectral area of the wavelength region of 380 to 520 nm and 620 to 780 nm with respect to the total recorded spectrum area was found to be 80%.
The solution in each syringe was distilled and the amount of alcohol recovered was measured.
The above operation was performed three times in consideration of measurement errors.
The results are summarized in Table 1.
In the table, symbols a to j represent the following plants, and “area ratio” is the ratio (%) of the spectral area of the wavelength region of 380 to 520 nm and 620 to 780 nm with respect to the total spectrum area recorded in the emission spectrum. Represents.
a: ginkgo leaf, b: cycad leaf, c: rice leaf, d: brookberry, e: gray algae, f: crypt algae, g: fresh seaweed, h: euglena algae, i: chloracunion algae, j : Blue-green algae

result:
In a closed system containing a solid-liquid mixture prepared by saccharification of rice containing starch, yeast and plant chloroplasts of various plants (a to j), in the wavelength range of 380 to 520 nm and 620 to 780 nm. Irradiation of light from a light emitting diode with a total light emission amount of 80% produced about 3.8 to 3.9 mL of ethanol using any plant chloroplast (Examples 1 to 10). However, this was about a 1.3 times increase in yield compared to the case where no plant chloroplast was used (Comparative Example 1).

Examples 11 to 20 and Comparative Example 2
217 g of the solid-liquid mixture (wheat) containing sugar produced in Production Example 2 and 3 g of yeast were stirred with a mixer to prepare 220 g of a solid-liquid mixture (wheat) containing sugar containing yeast.
The prepared solid-liquid mixture (wheat) containing sugar containing yeast is divided into 11 100 mL beakers of 20 g each, and 10 types of different plant pulverized products are added to each beaker by 1 g and stirred, and then each solution is transparent glass. A 100 mL syringe, which is a manufactured container, was injected (Examples 11 to 20).
As a comparison, 20 g of a solid-liquid mixture (wheat) containing sugar containing yeast without plant pulverized product was injected into a 100 mL syringe (Comparative Example 2).
The eleven syringes prepared above contain light from a total of 600 light emitting diodes composed of 200 white light emitting diodes, 200 red light emitting diodes, 100 yellow light emitting diodes and 100 indigo light emitting diodes (36 watts in total). / Hour) for 24 hours. The oxygen concentration of the gas collected from each syringe was measured with an oxygen concentration meter. Further, the emission spectrum of the light emitting diode was measured, and the ratio of the spectral areas of the wavelength regions of 380 to 520 nm and 620 to 780 nm with respect to the total recorded spectrum area was determined to be 77%.
The solution in each syringe was distilled and the amount of alcohol recovered was measured.
The above operation was performed three times in consideration of measurement errors.
The results are summarized in Table 2.
In the table, symbols a to j represent the same plants as shown above, and “area ratio” is the spectral area of the wavelength region of 380 to 520 nm and 620 to 780 nm with respect to the total spectral area recorded in the emission spectrum. Represents the ratio (%).

result:
In a sealed system containing a solid-liquid mixture prepared by saccharification of wheat containing starch, yeast and plant chloroplasts of various plants (a to j), in the wavelength range of 380 to 520 nm and 620 to 780 nm. Irradiation with light from a light emitting diode with a total light emission amount of 77% produced about 3.8 to 3.9 mL of ethanol in any plant chloroplast (Examples 11 to 20). However, this was about a 1.3-fold increase in yield compared to the case where no plant chloroplast was used (Comparative Example 2).

Examples 21 to 30 and Comparative Example 3
217 g of the solid-liquid mixture (corn) containing saccharide produced in Production Example 3 and 3 g of yeast were stirred with a mixer to prepare 220 g of a solid-liquid mixture (corn) containing sugar containing yeast.
The prepared solid-liquid mixture (corn) containing sugar containing yeast is divided into 11 100 mL beakers of 20 g each, 10 g of different plant pulverized products are added to each beaker by 1 g and stirred, and then each solution is transparent glass. It was injected into a 100 mL syringe, which is a manufactured container (Examples 21 to 30).
As a comparison, 20 g of a solid-liquid mixture (corn) containing yeast-containing sugar without plant pulverized product was injected into a 100 mL syringe (Comparative Example 3).
The eleven syringes prepared above contain 200 white light emitting diodes, 200 red light emitting diodes, 100 orange light emitting diodes, and 100 purple light emitting diodes, for a total of 600 light emitting diodes (36 watts / total). ) For 24 hours. The oxygen concentration of the gas collected from each syringe was measured with an oxygen concentration meter. Further, the emission spectrum of the light emitting diode was measured, and the ratio of the spectral areas of the wavelength regions of 380 to 520 nm and 620 to 780 nm with respect to the total recorded spectrum area was found to be 85%.
The solution in each syringe was distilled and the amount of alcohol recovered was measured.
The above operation was performed three times in consideration of measurement errors.
The results are summarized in Table 3.
In the table, symbols a to j represent the same plants as shown above, and “area ratio” is the spectral area of the wavelength region of 380 to 520 nm and 620 to 780 nm with respect to the total spectral area recorded in the emission spectrum. Represents the ratio (%).

result:
In a closed system containing a solid-liquid mixture prepared by saccharification of corn containing starch, yeast and plant chloroplasts of various plants (a to j), in the wavelength range of 380 to 520 nm and 620 to 780 nm. Irradiation of light from a light emitting diode with a total light emission amount of 85% produced 4.3 to 4.5 mL of ethanol in any plant chloroplast (Examples 21 to 30). However, this was about a 1.3 times increase in yield compared to the case where no plant chloroplast was used (Comparative Example 3).

Examples 31 to 40 and Comparative Example 4
217 g of the solid-liquid mixture (corn) containing saccharide produced in Production Example 3 and 3 g of yeast were stirred with a mixer to prepare 220 g of a solid-liquid mixture (corn) containing sugar containing yeast.
The prepared solid-liquid mixture (corn) containing sugar containing yeast was divided into 11 100 mL beakers of 20 g each, 10 g of different plant pulverized products were added to each beaker by 1 g and stirred, and then each beaker was sealed. I put it in the box.
A fermentation experiment was conducted separately from the above experiment, and carbon dioxide released from the fermentation experiment was introduced into each beaker through a tube so as to be mixed at the liquid level of each beaker in the sealed box (Examples 31 to 40). ).
For comparison, a beaker containing 20 g of a solid-liquid mixture (corn) containing sugar containing yeast and not containing plant pulverized material was placed in the sealed box (Comparative Example 4).
In each of the above beakers, light of a total of 600 light emitting diodes composed of 200 white light emitting diodes, 200 red light emitting diodes, 100 orange light emitting diodes, and 100 purple light emitting diodes (36 watts / hour in total) 24 Irradiated for hours. The emission spectrum of the light emitting diode was measured, and the ratio of the spectral areas in the wavelength regions of 380 to 520 nm and 620 to 780 nm with respect to the total recorded spectrum area was found to be 85%.
The solution in each syringe was distilled and the amount of alcohol recovered was measured.
The above operation was performed three times in consideration of measurement errors.
The results are summarized in Table 4.
In the table, symbols a to j represent the same plants as shown above, and “area ratio” is the spectral area of the wavelength region of 380 to 520 nm and 620 to 780 nm with respect to the total spectral area recorded in the emission spectrum. Represents the ratio (%).

result:
In a closed system containing a solid-liquid mixture prepared by saccharification of corn containing starch, yeast and plant chloroplasts of various plants (a to j), additional carbon dioxide is added and 380 to Irradiation of light from a light emitting diode with a total light emission amount of 85% in the wavelength range of 520 nm and 620 to 780 nm produces about 5.3 to 5.6 mL of ethanol in any plant chloroplast. (Examples 31 to 40), which is about a 1.6-fold increase in yield compared to the case where no plant chloroplast was used (Comparative Example 4), and further carbon dioxide. Compared to Examples 21 to 30 in which no was added, the yield was increased about 1.24 times.

Production Example 4: Production of a solid-liquid mixture containing sugar (rice) 10 g of amylase (mixture of alpha amylase and glucoamylase) and 525 g of water were added to 530 g of rice flour purchased from a food store, stirred with a mixer, hot water roasted, and 70 ° C. Furthermore, 1065 g of a solid-liquid mixture (rice) containing sugar was prepared by saccharifying the rice flour over 12 hours while maintaining at 80 ° C.

Examples 41 to 57 and Comparative Example 5
355 g of the solid-liquid mixture (rice) containing saccharide produced in Production Example 4 and 5 g of yeast were stirred with a mixer to prepare 360 g of a solid-liquid mixture (rice) containing sugar containing yeast.
The prepared solid-liquid mixture (rice) containing sugar containing yeast was divided into 18 100 mL beakers of 20 g each, and 17 types of pulverized products of different plants were added to each beaker by 1 g and stirred. A 100 mL syringe, which is a made container, was injected (Examples 41 to 57).
For comparison, 20 g of a solid-liquid mixture (rice) containing sugar containing yeast without adding plant pulverized material was injected into a 100 mL syringe (Comparative Example 5).
The 18 syringes prepared above were irradiated with light from a light emitting diode composed of 600 white light emitting diodes (36 watts / hour in total) for 24 hours. The oxygen concentration of the gas collected from each syringe was measured with an oxygen concentration meter. Further, the emission spectrum of the light emitting diode was measured, and the ratio of the spectral area of the wavelength region of 380 to 520 nm and 620 to 780 nm with respect to the total recorded spectrum area was found to be 80%.
The solution in each syringe was distilled and the amount of alcohol recovered was measured.
The above operation was performed three times in consideration of measurement errors.
The results are summarized in Table 5.
In the table, the symbols a to q represent the following plants, and the “area ratio” is the ratio (%) of the spectral area of the wavelength region of 380 to 520 nm and 620 to 780 nm with respect to the total spectral area recorded in the emission spectrum. Represents.
a: ginkgo leaf, b: cycad leaf, c: rice leaf, d: brookberry, e: gray algae, f: crypt algae, g: fresh seaweed, h: euglena algae, i: chloracunion algae, j : Blue-green algae, k: sphagnum, l: black moss, m: sphagnum, n: scale moss, o: mushroom, p: hornwort, q: cyanobacteria

result:
In a closed system containing a solid-liquid mixture prepared by saccharification of rice containing starch, yeast and plant chloroplasts of various plants (a to q), in the wavelength range of 380 to 520 nm and 620 to 780 nm. Irradiation of light from a light emitting diode with a total light emission amount of 80% produced about 3.8 to 3.9 mL of ethanol in any plant chloroplast (Examples 41 to 57). However, this was about a 1.3 times increase in yield compared to the case where no plant chloroplast was used (Comparative Example 5).

Examples 58 to 74 and Comparative Example 6
355 g of the solid-liquid mixture (rice) containing saccharide produced in Production Example 4 and 5 g of yeast were stirred with a mixer to prepare 360 g of a solid-liquid mixture (rice) containing sugar containing yeast.
The prepared solid-liquid mixture (rice) containing sugar containing yeast was divided into 18 100 mL beakers of 20 g each, and 17 types of pulverized products of different plants were added to each beaker by 1 g and stirred. A 100 mL syringe, which is a made container, was injected (Examples 58 to 74).
As a comparison, 20 g of a solid-liquid mixture (rice) containing sugar containing yeast without plant pulverized product was injected into a 100 mL syringe (Comparative Example 6).
The 18 syringes prepared above were irradiated with light (total 36 watts / hour) from a total of 600 light emitting diodes composed of 200 red light emitting diodes, 200 blue light emitting diodes and 200 green light emitting diodes for 24 hours. did. The oxygen concentration of the gas collected from each syringe was measured with an oxygen concentration meter. Further, the emission spectrum of the light emitting diode was measured, and the ratio of the spectral area of the wavelength region of 380 to 520 nm and 620 to 780 nm with respect to the total recorded spectrum area was found to be 74%.
The solution in each syringe was distilled and the amount of alcohol recovered was measured.
The above operation was performed three times in consideration of measurement errors.
The results are summarized in Table 6.
In the table, the symbols a to q represent the same plants as shown above, and the “area ratio” is the spectral area of the wavelength region of 380 to 520 nm and 620 to 780 nm with respect to the total spectral area recorded in the emission spectrum. Represents the ratio (%).

result:
In a closed system containing a solid-liquid mixture prepared by saccharification of rice containing starch, yeast and plant chloroplasts of various plants (a to q), in the wavelength range of 380 to 520 nm and 620 to 780 nm. Irradiation of light from a light emitting diode with a total light emission amount of 74% produced about 3.8 to 3.9 mL of ethanol in any plant chloroplast (Examples 58 to 74). However, this was an increase in yield of about 1.3 times compared to the case where no plant chloroplast was used (Comparative Example 6).

Examples 75 to 91 and Comparative Example 7
355 g of the solid-liquid mixture (rice) containing saccharide produced in Production Example 4 and 5 g of yeast were stirred with a mixer to prepare 360 g of a solid-liquid mixture (rice) containing sugar containing yeast.
The prepared solid-liquid mixture (rice) containing sugar containing yeast was divided into 18 100 mL beakers of 20 g each, and 17 types of pulverized products of different plants were added to each beaker by 1 g and stirred. A 100 mL syringe, which is a made container, was injected (Examples 75 to 91).
For comparison, 20 g of a solid-liquid mixture (rice) containing sugar containing yeast without adding plant pulverized material was injected into a 100 mL syringe (Comparative Example 7).
The 18 syringes prepared above were irradiated with light (total 36 watts / hour) of a total of 600 light emitting diodes composed of 300 red light emitting diodes and 300 blue light emitting diodes for 24 hours. The oxygen concentration of the gas collected from each syringe was measured with an oxygen concentration meter. Further, the emission spectrum of the light emitting diode was measured, and the ratio of the spectral areas of the wavelength regions of 380 to 520 nm and 620 to 780 nm with respect to the total recorded spectrum area was found to be 100%.
The solution in each syringe was distilled and the amount of alcohol recovered was measured.
The above operation was performed three times in consideration of measurement errors.
The results are summarized in Table 7.
In the table, the symbols a to q represent the same plants as shown above, and the “area ratio” is the spectral area of the wavelength region of 380 to 520 nm and 620 to 780 nm with respect to the total spectral area recorded in the emission spectrum. Represents the ratio (%).

result:
In a closed system containing a solid-liquid mixture prepared by saccharification of rice containing starch, yeast and plant chloroplasts of various plants (a to q), in the wavelength range of 380 to 520 nm and 620 to 780 nm. Irradiation of light from a light emitting diode with a total light emission amount of 100% produced about 3.8 to 3.9 mL of ethanol in any plant chloroplast (Examples 75 to 91). However, this was an increase in yield of about 1.3 times compared to the case where no plant chloroplast was used (Comparative Example 7).

Examples 92 to 108 and Comparative Example 8
355 g of the solid-liquid mixture (rice) containing saccharide produced in Production Example 4 and 5 g of yeast were stirred with a mixer to prepare 360 g of a solid-liquid mixture (rice) containing sugar containing yeast.
The prepared solid-liquid mixture (rice) containing sugar containing yeast was divided into 18 100 mL beakers of 20 g each, and 17 types of pulverized products of different plants were added to each beaker by 1 g and stirred. A 100 mL syringe, which is a manufactured container, was injected (Examples 92 to 108).
As a comparison, 20 g of a solid-liquid mixture (rice) containing sugar containing yeast without plant pulverized product was injected into a 100 mL syringe (Comparative Example 8).
The 18 syringes prepared above were irradiated with light (total 36 watts / hour) of a total of 600 light emitting diodes composed of 300 blue light emitting diodes and 300 green light emitting diodes for 24 hours. The oxygen concentration of the gas collected from each syringe was measured with an oxygen concentration meter. Further, the emission spectrum of the light emitting diode was measured, and the ratio of the spectral areas of the wavelength regions of 380 to 520 nm and 620 to 780 nm with respect to the total recorded spectrum area was found to be 60%.
The solution in each syringe was distilled and the amount of alcohol recovered was measured.
The above operation was performed three times in consideration of measurement errors.
The results are summarized in Table 8.
In the table, the symbols a to q represent the same plants as shown above, and the “area ratio” is the spectral area of the wavelength region of 380 to 520 nm and 620 to 780 nm with respect to the total spectral area recorded in the emission spectrum. Represents the ratio (%).

result:
In a closed system containing a solid-liquid mixture prepared by saccharification of rice containing starch, yeast and plant chloroplasts of various plants (a to q), in the wavelength range of 380 to 520 nm and 620 to 780 nm. When irradiated with light from a light emitting diode whose total light emission amount is 60%, except for the case of q cyanobacteria (Example 108), 3.3 to 3.4 mL of any plant chloroplast is used. About ethanol was produced (Examples 92 to 107), which was about a 1.1-fold increase in yield compared to the case where no plant chloroplast was used (Comparative Example 8). In the case of q cyanobacteria (Example 108), the yield increased about 1.23 times compared to Comparative Example 8.

Examples 109 to 125 and Comparative Example 9
355 g of the solid-liquid mixture (rice) containing saccharide produced in Production Example 4 and 5 g of yeast were stirred with a mixer to prepare 360 g of a solid-liquid mixture (rice) containing sugar containing yeast.
The prepared solid-liquid mixture (rice) containing sugar containing yeast was divided into 18 100 mL beakers of 20 g each, and 17 types of pulverized products of different plants were added to each beaker by 1 g and stirred. A 100 mL syringe, which is a manufactured container, was injected (Examples 109 to 125).
For comparison, 20 g of a solid-liquid mixture (rice) containing sugar containing yeast without adding plant pulverized material was injected into a 100 mL syringe (Comparative Example 9).
The 18 syringes prepared above were irradiated with light (total 36 watts / hour) of 600 light-emitting diodes composed of 300 red light-emitting diodes and 300 green light-emitting diodes for 24 hours. The oxygen concentration of the gas collected from each syringe was measured with an oxygen concentration meter. Further, the emission spectrum of the light emitting diode was measured, and the ratio of the spectral areas of the wavelength regions of 380 to 520 nm and 620 to 780 nm with respect to the total recorded spectrum area was found to be 60%.
The solution in each syringe was distilled and the amount of alcohol recovered was measured.
The above operation was performed three times in consideration of measurement errors.
The results are summarized in Table 9.
In the table, the symbols a to q represent the same plants as shown above, and the “area ratio” is the spectral area of the wavelength region of 380 to 520 nm and 620 to 780 nm with respect to the total spectral area recorded in the emission spectrum. Represents the ratio (%).

result:
In a closed system containing a solid-liquid mixture prepared by saccharification of rice containing starch, yeast and plant chloroplasts of various plants (a to q), in the wavelength range of 380 to 520 nm and 620 to 780 nm. When irradiated with light from a light emitting diode with a total light emission amount of 60%, except for the case of q cyanobacteria (Example 125), 3.2 to 3.3 mL of any plant chloroplast is used. About 109% of ethanol was produced (Examples 109 to 124), which was about 1.1 times higher yield than when no plant chloroplast was used (Comparative Example 9). . In the case of q cyanobacteria (Example 125), the yield increased about 1.22 times compared to Comparative Example 9.

Examples 126 to 142 and Comparative Example 10
355 g of the solid-liquid mixture (rice) containing saccharide produced in Production Example 4 and 5 g of yeast were stirred with a mixer to prepare 360 g of a solid-liquid mixture (rice) containing sugar containing yeast.
The prepared solid-liquid mixture (rice) containing sugar containing yeast was divided into 18 100 mL beakers of 20 g each, and 17 types of pulverized products of different plants were added to each beaker by 1 g and stirred. A 100 mL syringe, which is a made container, was injected (Examples 126 to 142).
For comparison, 20 g of a solid-liquid mixture (rice) containing sugar containing yeast without pulverized plant was injected into a 100 mL syringe (Comparative Example 10).
The 18 syringes prepared above were irradiated with light from a light emitting diode composed of 600 blue light emitting diodes (36 watts / hour in total) for 24 hours. The oxygen concentration of the gas collected from each syringe was measured with an oxygen concentration meter. Further, the emission spectrum of the light emitting diode was measured, and the ratio of the spectral areas of the wavelength regions of 380 to 520 nm and 620 to 780 nm with respect to the total recorded spectrum area was found to be 100%.
The solution in each syringe was distilled and the amount of alcohol recovered was measured.
The above operation was performed three times in consideration of measurement errors.
The results are summarized in Table 10.
In the table, the symbols a to q represent the same plants as shown above, and the “area ratio” is the spectral area of the wavelength region of 380 to 520 nm and 620 to 780 nm with respect to the total spectral area recorded in the emission spectrum. Represents the ratio (%).

result:
In a closed system containing a solid-liquid mixture prepared by saccharification of rice containing starch, yeast and plant chloroplasts of various plants (a to q), in the wavelength range of 380 to 520 nm and 620 to 780 nm. Irradiation with light from a light emitting diode with a total light emission amount of 100% produced about 3.8 to 3.9 mL of ethanol in any plant chloroplast (Examples 126 to 142). However, this was about a 1.3-fold increase in yield compared to the case where no plant chloroplast was used (Comparative Example 10).

Examples 143 to 159 and Comparative Example 11
355 g of the solid-liquid mixture (rice) containing saccharide produced in Production Example 4 and 5 g of yeast were stirred with a mixer to prepare 360 g of a solid-liquid mixture (rice) containing sugar containing yeast.
The prepared solid-liquid mixture (rice) containing sugar containing yeast was divided into 18 100 mL beakers of 20 g each, and 17 types of pulverized products of different plants were added to each beaker by 1 g and stirred. A 100 mL syringe, which is a manufactured container, was injected (Examples 143 to 159).
For comparison, 20 g of a solid-liquid mixture (rice) containing a sugar containing yeast without adding plant pulverized material was injected into a 100 mL syringe (Comparative Example 11).
The 18 syringes prepared above were irradiated with light from a light emitting diode composed of 600 red light emitting diodes (36 watts / hour in total) for 24 hours. The oxygen concentration of the gas collected from each syringe was measured with an oxygen concentration meter. Further, the emission spectrum of the light emitting diode was measured, and the ratio of the spectral areas of the wavelength regions of 380 to 520 nm and 620 to 780 nm with respect to the total recorded spectrum area was found to be 100%.
The solution in each syringe was distilled and the amount of alcohol recovered was measured.
The above operation was performed three times in consideration of measurement errors.
The results are summarized in Table 11.
In the table, the symbols a to q represent the same plants as shown above, and the “area ratio” is the spectral area of the wavelength region of 380 to 520 nm and 620 to 780 nm with respect to the total spectral area recorded in the emission spectrum. Represents the ratio (%).

result:
In a closed system containing a solid-liquid mixture prepared by saccharification of rice containing starch, yeast and plant chloroplasts of various plants (a to q), in the wavelength range of 380 to 520 nm and 620 to 780 nm. Irradiation of light from a light emitting diode with a total light emission amount of 100% produced about 3.8 to 3.9 mL of ethanol in any plant chloroplast (Examples 143 to 159). However, this was about a 1.3 times increase in yield compared to the case where no plant chloroplast was used (Comparative Example 11).

Examples 160 to 176 and Comparative Example 12
355 g of the solid-liquid mixture (rice) containing saccharide produced in Production Example 4 and 5 g of yeast were stirred with a mixer to prepare 360 g of a solid-liquid mixture (rice) containing sugar containing yeast.
The prepared solid-liquid mixture (rice) containing sugar containing yeast was divided into 18 100 mL beakers of 20 g each, and 17 types of pulverized products of different plants were added to each beaker by 1 g and stirred. It was injected into a 100 mL syringe, which is a manufactured container (Examples 160 to 176).
As a comparison, 20 g of a solid-liquid mixture (rice) containing sugar containing yeast without plant pulverized product was injected into a 100 mL syringe (Comparative Example 12).
The 18 syringes prepared above were irradiated with light from a light emitting diode composed of 600 green light emitting diodes (36 watts / hour in total) for 24 hours. The oxygen concentration of the gas collected from each syringe was measured with an oxygen concentration meter. Further, the emission spectrum of the light emitting diode was measured, and the ratio of the spectral area of the wavelength region of 380 to 520 nm and 620 to 780 nm with respect to the total recorded spectrum area was found to be 20%.
The solution in each syringe was distilled and the amount of alcohol recovered was measured.
The above operation was performed three times in consideration of measurement errors.
The results are summarized in Table 12.
In the table, the symbols a to q represent the same plants as shown above, and the “area ratio” is the spectral area of the wavelength region of 380 to 520 nm and 620 to 780 nm with respect to the total spectral area recorded in the emission spectrum. Represents the ratio (%).

result:
In a closed system containing a solid-liquid mixture prepared by saccharification of rice containing starch, yeast and plant chloroplasts of various plants (a to q), in the wavelength range of 380 to 520 nm and 620 to 780 nm. When irradiated with light from a light-emitting diode with a total light emission amount of 20%, except for the case of q cyanobacteria (Example 176), 3.4 to 3.5 mL in any plant chloroplast. About ethanol was produced (Examples 160 to 175), which was a yield increase of about 1.15 times compared to the case where no plant chloroplast was used (Comparative Example 12). . In the case of q cyanobacteria (Example 176), the yield increased about 1.27 times compared to Comparative Example 12.

Examples 177 to 193 and Comparative Example 13
355 g of the solid-liquid mixture (rice) containing saccharide produced in Production Example 4 and 5 g of yeast were stirred with a mixer to prepare 360 g of a solid-liquid mixture (rice) containing sugar containing yeast.
The prepared solid-liquid mixture (rice) containing sugar containing yeast was divided into 18 100 mL beakers of 20 g each, and 17 types of pulverized products of different plants were added to each beaker by 1 g and stirred. A 100 mL syringe, which is a made container, was injected (Examples 177 to 193).
For comparison, 20 g of a solid-liquid mixture (rice) containing sugar containing yeast without pulverized plants was injected into a 100 mL syringe (Comparative Example 13).
The 18 syringes prepared above were irradiated with light from a light emitting diode composed of 600 yellow light emitting diodes (36 watts / hour in total) for 24 hours. The oxygen concentration of the gas collected from each syringe was measured with an oxygen concentration meter. Further, the emission spectrum of the light emitting diode was measured, and the ratio of the spectral area of the wavelength region of 380 to 520 nm and 620 to 780 nm with respect to the total recorded spectrum area was found to be 0%.
The solution in each syringe was distilled and the amount of alcohol recovered was measured.
The above operation was performed three times in consideration of measurement errors.
The results are summarized in Table 13.
In the table, the symbols a to q represent the same plants as shown above, and the “area ratio” is the spectral area of the wavelength region of 380 to 520 nm and 620 to 780 nm with respect to the total spectral area recorded in the emission spectrum. Represents the ratio (%).

result:
In a closed system containing a solid-liquid mixture prepared by saccharification of rice containing starch, yeast and plant chloroplasts of various plants (a to q), in the wavelength range of 380 to 520 nm and 620 to 780 nm. When irradiated with light from a light emitting diode with a total light emission amount of 0%, except for the case of q cyanobacteria (Example 193), 3.2 to 3.4 mL of any plant chloroplast was used. About ethanol was produced (Examples 177 to 192), which was an increase in yield of about 1.1 times compared to the case where no plant chloroplast was used (Comparative Example 13). . In the case of q cyanobacteria (Example 193), the yield increased about 1.22 times compared to Comparative Example 13.

Examples 194 to 210 and Comparative Example 14
355 g of the solid-liquid mixture (rice) containing saccharide produced in Production Example 4 and 5 g of yeast were stirred with a mixer to prepare 360 g of a solid-liquid mixture (rice) containing sugar containing yeast.
The prepared solid-liquid mixture (rice) containing sugar containing yeast was divided into 18 100 mL beakers of 20 g each, and 17 types of pulverized products of different plants were added to each beaker by 1 g and stirred. A 100 mL syringe, which is a made container, was injected (Examples 194 to 210).
For comparison, 20 g of a solid-liquid mixture (rice) containing sugar containing yeast without adding plant pulverized material was injected into a 100 mL syringe (Comparative Example 14).
The 18 syringes prepared above were irradiated with light from a light emitting diode composed of 600 purple light emitting diodes (36 watts / hour in total) for 24 hours. The oxygen concentration of the gas collected from each syringe was measured with an oxygen concentration meter. Further, the emission spectrum of the light emitting diode was measured, and the ratio of the spectral areas of the wavelength regions of 380 to 520 nm and 620 to 780 nm with respect to the total recorded spectrum area was found to be 100%.
The solution in each syringe was distilled and the amount of alcohol recovered was measured.
The above operation was performed three times in consideration of measurement errors.
The results are summarized in Table 14.
In the table, the symbols a to q represent the same plants as shown above, and the “area ratio” is the spectral area of the wavelength region of 380 to 520 nm and 620 to 780 nm with respect to the total spectral area recorded in the emission spectrum. Represents the ratio (%).

result:
In a closed system containing a solid-liquid mixture prepared by saccharification of rice containing starch, yeast and plant chloroplasts of various plants (a to q), in the wavelength range of 380 to 520 nm and 620 to 780 nm. When irradiated with light from a light emitting diode with a total light emission amount of 100%, except for the case of q cyanobacteria (Example 210), 3.8 to 4.0 mL when using any plant chloroplast. About ethanol was produced (Examples 194 to 209), which was about a 1.3-fold increase in yield compared to the case where no plant chloroplast was used (Comparative Example 14). In the case of q cyanobacteria (Example 210), the yield was increased by about 1.47 times compared to Comparative Example 14.

Examples 211 to 227 and Comparative Example 15
355 g of the solid-liquid mixture (rice) containing saccharide produced in Production Example 4 and 5 g of yeast were stirred with a mixer to prepare 360 g of a solid-liquid mixture (rice) containing sugar containing yeast.
The prepared solid-liquid mixture (rice) containing sugar containing yeast was divided into 18 100 mL beakers of 20 g each, and 17 types of pulverized products of different plants were added to each beaker by 1 g and stirred. A 100 mL syringe, which is a manufactured container, was injected (Examples 211 to 227).
As a comparison, 20 g of a solid-liquid mixture (rice) containing sugar containing yeast without plant pulverized product was injected into a 100 mL syringe (Comparative Example 15).
The 18 syringes prepared above were irradiated with light from a light emitting diode composed of 600 orange light emitting diodes (36 watts / hour in total) for 24 hours. The oxygen concentration of the gas collected from each syringe was measured with an oxygen concentration meter. Further, the emission spectrum of the light emitting diode was measured, and the ratio of the spectral areas of the wavelength regions of 380 to 520 nm and 620 to 780 nm with respect to the total recorded spectrum area was found to be 50%.
The solution in each syringe was distilled and the amount of alcohol recovered was measured.
The above operation was performed three times in consideration of measurement errors.
The results are summarized in Table 15.
In the table, the symbols a to q represent the same plants as shown above, and the “area ratio” is the spectral area of the wavelength region of 380 to 520 nm and 620 to 780 nm with respect to the total spectral area recorded in the emission spectrum. Represents the ratio (%).

result:
In a closed system containing a solid-liquid mixture prepared by saccharification of rice containing starch, yeast and plant chloroplasts of various plants (a to q), in the wavelength range of 380 to 520 nm and 620 to 780 nm. When irradiated with light from a light emitting diode with a total light emission amount of 50%, except for the case of q cyanobacteria (Example 227), 3.4 to 3.5 mL when using any plant chloroplast. About ethanol was produced (Examples 211 to 226), which was a yield increase of about 1.15 times compared to the case where no plant chloroplast was used (Comparative Example 15). . In the case of q cyanobacteria (Example 227), the yield increased about 1.26 times compared to Comparative Example 15.

Examples 228 to 244 and Comparative Example 16
355 g of the solid-liquid mixture (rice) containing saccharide produced in Production Example 4 and 5 g of yeast were stirred with a mixer to prepare 360 g of a solid-liquid mixture (rice) containing sugar containing yeast.
The prepared solid-liquid mixture (rice) containing sugar containing yeast was divided into 18 100 mL beakers of 20 g each, and 17 types of pulverized products of different plants were added to each beaker by 1 g and stirred, and then each beaker was sealed. I put it in the box.
A fermentation experiment was conducted separately from the above experiment, and carbon dioxide released from the fermentation experiment was introduced into each beaker through a tube so as to be mixed at the liquid level of each beaker in the sealed box (Examples 228 to 244). ).
For comparison, a beaker containing 20 g of a solid-liquid mixture (rice) containing sugar containing yeast without adding plant ground was placed in the sealed box (Comparative Example 16).
Each beaker was irradiated with light from a light emitting diode composed of 600 white light emitting diodes (36 watts / hour in total) for 24 hours. The emission spectrum of the light-emitting diode was measured, and the ratio of the spectral area in the wavelength region of 380 to 520 nm and 620 to 780 nm to the total recorded spectral area was determined to be 80%.
The solution in each syringe was distilled and the amount of alcohol recovered was measured.
The above operation was performed three times in consideration of measurement errors.
The results are summarized in Table 16.
In the table, symbols a to j represent the same plants as shown above, and “area ratio” is the spectral area of the wavelength region of 380 to 520 nm and 620 to 780 nm with respect to the total spectral area recorded in the emission spectrum. Represents the ratio (%).

result:
In a closed system containing a solid-liquid mixture prepared by saccharification of rice containing starch, yeast and plant chloroplasts of various plants (a to q), additional carbon dioxide is added and 380 to When irradiating light from a light emitting diode with a total light emission amount of 80% in the wavelength region of 520 nm and 620 to 780 nm, except for the case of q cyanobacteria (Example 244), when using any plant chloroplast However, about 5.5 to 5.6 mL of ethanol was produced (Examples 228 to 243), which was about 1.times. Compared to the case where no plant chloroplast was used (Comparative Example 16). The yield increased by 7 times, and the yield increased by about 1.4 times compared to Examples 41 to 57 in which no additional carbon dioxide was added. Further, in the case of q cyanobacteria (Example 244), the yield was increased about twice as compared with Example 57 in which no additional carbon dioxide was added.

Production Example 5: Production of a solid-liquid mixture containing sugar (sugar cane) Sugar cane was purchased from a food store and washed. The liquid obtained by removing the sugar from the sugar cane juice was stirred to prepare 2000 g of molasses (a solid-liquid mixture containing sugar (sugar cane)).

Production Example 6: Production of solid-liquid mixture containing sugar (yellow peach) Yellow peach was purchased from a food store and washed. Yellow peach was pulverized to prepare a solid-liquid mixture (yellow peach) containing sugar without adding 2000 g of water.

Examples 245 to 254 and Comparative Example 17
660 g of the molasses produced in Production Example 5 (solid-liquid mixture containing sugar (sugar cane)) and 10 g of yeast and 870 g of water were stirred with a mixer, and the molasses containing sugar (solid-liquid mixture containing sugar (sugar cane)) 1540 Was prepared.
The prepared molasses containing yeast (solid-liquid mixture (sugar cane) containing sugar) was divided into 11 300 mL beakers of 140 g each, and 10 g of different plant pulverized products were added to each beaker by 5 g and stirred. The solution was poured into a 300 mL syringe, a clear glass container (Examples 245 to 254).
For comparison, 140 g of yeast molasses (solid-liquid mixture containing sugar (sugar cane)) without pulverized plant was injected into a 300 mL syringe (Comparative Example 17).
The 11 syringes prepared above were irradiated with light from a total of 600 light-emitting diodes composed of 200 white light-emitting diodes, 200 red light-emitting diodes and 200 blue light-emitting diodes (36 watts / hour in total) for 24 hours. did. The oxygen concentration of the gas collected from each syringe was measured with an oxygen concentration meter. Further, the emission spectrum of the light emitting diode was measured, and the ratio of the spectral areas of the wavelength regions of 380 to 520 nm and 620 to 780 nm with respect to the total recorded spectrum area was found to be 93%.
The solution in each syringe was distilled and the amount of alcohol recovered was measured.
The above operation was performed three times in consideration of measurement errors.
The results are summarized in Table 17.
In the table, symbols a to j represent the following plants, and “area ratio” is the ratio (%) of the spectral area of the wavelength region of 380 to 520 nm and 620 to 780 nm with respect to the total spectrum area recorded in the emission spectrum. Represents.
a: ginkgo leaf, b: cycad leaf, c: rice leaf, d: brookberry, e: gray algae, f: crypt algae, g: fresh seaweed, h: euglena algae, i: chloracunion algae, j : Blue-green algae

result:
380-520 nm and 620-780 nm in a closed system containing a solid-liquid mixture containing sugar prepared by grinding sugar cane, a vegetable, yeast and plant chloroplasts of various plants (a to j) Irradiation of light from a light emitting diode with a total light emission amount of 93% in the wavelength region produced 3.0 to 3.6 mL of ethanol in any plant chloroplast (Example) 245 to 254), which was about 1.65 times higher yield than when no plant chloroplast was used (Comparative Example 17).

Examples 255 to 264 and Comparative Example 18
A solid-liquid mixture (yellow peach) 1540 containing sugar containing yeast was prepared by stirring 660 g of the solid-liquid mixture (yellow peach) containing sugar produced in Production Example 6 and 10 g of yeast and 870 g of water with a mixer.
The prepared solid-liquid mixture (yellow peach) containing sugar containing yeast is divided into 11 300 mL beakers of 140 g each, 10 g of different plant pulverized products are added to each beaker by 5 g and stirred, and then each solution is transparent. A 300 mL syringe, a glass container, was injected (Examples 255 to 264).
As a comparison, 140 g of a solid-liquid mixture (yellow peach) containing sugar containing yeast without plant pulverized product was injected into a 300 mL syringe (Comparative Example 18).
A total of 600 light emitting diodes (36 watts in total) composed of 200 red light emitting diodes, 200 green light emitting diodes, 100 purple light emitting diodes, and 100 indigo blue light emitting diodes in the 11 syringes prepared above. / Hour) for 24 hours. The oxygen concentration of the gas collected from each syringe was measured with an oxygen concentration meter. Further, the emission spectrum of the light emitting diode was measured, and the ratio of the spectral areas of the wavelength regions of 380 to 520 nm and 620 to 780 nm with respect to the total recorded spectrum area was found to be 73%.
The solution in each syringe was distilled and the amount of alcohol recovered was measured.
The above operation was performed three times in consideration of measurement errors.
The results are summarized in Table 18.
In the table, symbols a to j represent the following plants, and “area ratio” is the ratio (%) of the spectral area of the wavelength region of 380 to 520 nm and 620 to 780 nm with respect to the total spectrum area recorded in the emission spectrum. Represents.
a: ginkgo leaf, b: cycad leaf, c: rice leaf, d: brookberry, e: gray algae, f: crypt algae, g: fresh seaweed, h: euglena algae, i: chloracunion algae, j : Blue-green algae

result:
A solid-liquid mixture containing sugar prepared by pulverizing yellow peach, which is a fruit, in a closed system containing yeast and plant chloroplasts of various plants (a to j), 380 to 520 nm and 620 to Irradiation with light from a light emitting diode with a total emission amount of 73% in the wavelength region of 780 nm produced 3.3 to 3.9 mL of ethanol in any plant chloroplast (implementation) Examples 255 to 264) increased the yield by about 1.64 times compared to the case where no plant chloroplast was used (Comparative Example 18).

The ethanol production method of the present invention does not emit carbon dioxide, and thus is said to be a useful method in terms of CO ₂ reduction. Moreover, since the manufacturing method of ethanol of this invention can obtain oxygen as a by-product, this can also be utilized as an effective resource.
In addition, since the ethanol production method of the present invention can take in carbon dioxide from the outside and convert it into ethanol, it can also be a useful ethanol production method from the viewpoint of fixing carbon dioxide. .

Claims

A production process for producing ethanol by irradiating light from a light-emitting diode in a sealed system containing a solid-liquid mixture containing sugar, yeast and plant chloroplasts, and recovering the ethanol produced from the system outside the system A method for producing ethanol comprising a recovery step,
In the generating step,
a) a reaction in which a solid-liquid mixture containing sugar is fermented with yeast to produce ethanol;
b) Reaction of photosynthesis of carbon dioxide generated during fermentation in a) by light irradiation of plant chloroplasts and light emitting diodes to sugar, and c) fermentation of sugar obtained in b) with yeast. The reaction for producing ethanol proceeds in parallel, and the solid-liquid mixture containing sugar is saccharified by pyrolysis or hydrolysis of cellulose or hemicellulose, or rice, wheat, straw or corn containing starch Saccharify with koji, malt or enzyme, crush vegetables and / or fruits, or crush vegetables and / or fruits, then heat concentration method, freeze concentration method, reverse osmosis The plant chloroplast is prepared by concentrating by one or more concentration methods selected from a concentration method and a vacuum concentration method, and the plant chloroplast is a seed plant, a fern plant, an algae, a moss plant, a bacterium Or a mixture of these Ethanol production process is a plant chloroplast things.
2. The method for producing ethanol according to claim 1, wherein the light from the light emitting diode is light in which the total light emission amount in the wavelength regions of 380 to 520 nm and 620 to 780 nm is 70% or more of the light emission amount over the entire wavelength region.
The method for producing ethanol according to claim 1 or 2, wherein further carbon dioxide is added into the sealed system.
The method for producing ethanol according to any one of claims 1 to 3, wherein the recovery step is by distillation.